期刊文献+
共找到9篇文章
< 1 >
每页显示 20 50 100
秋水仙碱诱导栝楼四倍体 被引量:9
1
作者 吴玉香 杨福红 +2 位作者 赵晓明 何永波 李鹏飞 《核农学报》 CAS CSCD 北大核心 2009年第3期418-422,共5页
采用改良L.D.Cua法诱导短把栝楼获得四倍体,研究了0.2%浓度的秋水仙碱诱导短把栝楼染色体倍性变异的最佳处理时间、四倍体栝楼与原栝楼的形态特征及其四倍体与二倍体的细胞学鉴定。结果表明:0.2%秋水仙素的最佳处理时间为72 h,诱变率达8... 采用改良L.D.Cua法诱导短把栝楼获得四倍体,研究了0.2%浓度的秋水仙碱诱导短把栝楼染色体倍性变异的最佳处理时间、四倍体栝楼与原栝楼的形态特征及其四倍体与二倍体的细胞学鉴定。结果表明:0.2%秋水仙素的最佳处理时间为72 h,诱变率达82.1%。与二倍体相比,四倍体表现为:生长变缓;叶片增大、皱缩和变厚、叶形指数减小;叶片保卫细胞增大,气孔密度降低,叶绿体数目增加,叶色加深。根尖压片表明:二倍体的染色体数目为2n=66,大部分四倍体植株的染色体数目为2n=132,M2代部分四倍体植株也存在嵌合现象。 展开更多
关键词 栝楼 四倍体 改良l.d.cua
下载PDF
秋水仙素诱导美洲商陆四倍体的研究 被引量:5
2
作者 常建忠 吴玉香 +1 位作者 赵晓明 闫凤霞 《中国农学通报》 CSCD 北大核心 2009年第4期35-38,共4页
利用改良L.D.Cua法对美洲商陆进行多倍体诱变,在秋水仙碱浓度为0.2%条件下,设置24h、48h和72h三个诱变时间,并对所得到的变异株和对照株进行形态学、细胞学等方面的比较研究,结果表明:诱变48h效果最好;与对照相比,变异株的叶片厚度增加4... 利用改良L.D.Cua法对美洲商陆进行多倍体诱变,在秋水仙碱浓度为0.2%条件下,设置24h、48h和72h三个诱变时间,并对所得到的变异株和对照株进行形态学、细胞学等方面的比较研究,结果表明:诱变48h效果最好;与对照相比,变异株的叶片厚度增加43.75%;叶形指数减少37.95%;气孔纵横径分别增加52.94%和73.01%;保卫细胞内叶绿体数目增加20.91%;花直径增加22.96%;花粉粒直径增加22.47%;果实直径增加23.66%。对变异株进行花粉母细胞减数分裂染色体鉴定,小孢子细胞的染色体数为n=2x=36,而对照小孢子细胞的染色体数为n=x=18,证明变异株为四倍体。 展开更多
关键词 美洲商陆 改良l.d.cua 同源四倍体
下载PDF
秋水仙素处理对连翘生长的影响 被引量:3
3
作者 王艳英 邓传远 +1 位作者 赵晓明 李海平 《蚌埠学院学报》 2014年第1期20-23,共4页
以二倍体连翘(Forsythia suspense(Thund.)Vahl)为材料,采用改良L.D.Cua法在0.2%秋水仙素浓度下对连翘幼苗顶芽进行不同时间的处理,观察处理组与对照组在形态学上的差异,探索秋水仙素诱导连翘的最佳处理时间。结果表明:连翘经秋水仙素... 以二倍体连翘(Forsythia suspense(Thund.)Vahl)为材料,采用改良L.D.Cua法在0.2%秋水仙素浓度下对连翘幼苗顶芽进行不同时间的处理,观察处理组与对照组在形态学上的差异,探索秋水仙素诱导连翘的最佳处理时间。结果表明:连翘经秋水仙素处理后,形态上与对照组存在显著差异,表现为植株变矮、叶片变厚、叶型指数变小,其中48 h差异最显著。并且得到了四倍体的连翘,其表现为叶下表皮气孔器变大、气孔密度下降、保卫细胞内叶绿体的含量增加。 展开更多
关键词 连翘 改良L D Cua法 秋水仙素
下载PDF
合成高纯度丙交酯的方法改进 被引量:3
4
作者 唐拾贵 蒋云晨 +2 位作者 孙雁龙 叶飞 郭凯 《合成化学》 CAS CSCD 北大核心 2012年第6期767-770,共4页
用1,4-去水山梨醇固定羧基,D,L-乳酸(2)经催化聚合制得低聚乳酸(5);5经热裂解合成了丙交酯(3)。最佳反应条件为:2 960 mmol,n(OH):n(2)=1:15,辛酸亚锡1.2%(以2质量计算),在3.0 kPa下由90℃升温至160.0℃(2.0 h);在0.4 kPa下由160℃升温... 用1,4-去水山梨醇固定羧基,D,L-乳酸(2)经催化聚合制得低聚乳酸(5);5经热裂解合成了丙交酯(3)。最佳反应条件为:2 960 mmol,n(OH):n(2)=1:15,辛酸亚锡1.2%(以2质量计算),在3.0 kPa下由90℃升温至160.0℃(2.0 h);在0.4 kPa下由160℃升温至180.0℃(0.5 h),于180.0℃反应5.0 h制得5;由180.0℃逐渐升温至230.0℃进行5的裂解反应,收集180.0℃~230.0℃馏分得1,收率96.1%,纯度97.5%。 展开更多
关键词 D L-乳酸 1 4-去水山梨醇 丙交酯 合成 方法改进
下载PDF
神经生长液对衰老模型小鼠学习记忆能力的影响 被引量:11
5
作者 柯开富 周鸣鸣 +1 位作者 强亮 丁斐 《中国临床康复》 CSCD 2003年第25期3444-3445,F003,共3页
目的:探讨神经生长液对D-半乳糖衰老模型小鼠学习记忆能力及海马神经丝蛋白表达的影响,为应用神经生长液防治神经退行性变疾病提供实验依据。方法:D-半乳糖致小鼠衰老模型,以Y型电迷宫检测其学习记忆能力;以抗低分子量神经丝蛋白(NF-L)... 目的:探讨神经生长液对D-半乳糖衰老模型小鼠学习记忆能力及海马神经丝蛋白表达的影响,为应用神经生长液防治神经退行性变疾病提供实验依据。方法:D-半乳糖致小鼠衰老模型,以Y型电迷宫检测其学习记忆能力;以抗低分子量神经丝蛋白(NF-L)抗体,采用WesternBlot法观察海马NF-L的表达。结果:与正常对照组相比(65.8±5.8),D-半乳糖衰老模型小鼠达到学会标准所用的次数明显增加(97.8±13.9,t=7.355,P<0.01),低、高剂量神经生长液可明显减少衰老模型小鼠的次数(80.1±9.3,67.7±8.2,t=3.6662,6.4609,P均<0.01)。衰老小鼠海马NF-L表达量明显下降;神经生长液防止衰老所致NF-L表达量减少。结论:神经生长液可改善D-半乳糖模型小鼠的学习记忆能力,防止衰老小鼠海马NF-L表达的减少。 展开更多
关键词 衰老 神经生长液 中药制剂 神经丝蛋白 学习记忆能力 动物实验
下载PDF
牛膝多倍体的诱变及鉴定 被引量:1
6
作者 李鹏飞 赵晓明 《山西农业大学学报(自然科学版)》 CAS 2009年第2期115-118,共4页
通过诱变牛膝多倍体,增加新的药用种质资源,为培育牛膝新品系奠定基础。以牛膝(Achyranthes bidentata Blume)为实验材料,研究了秋水仙素的诱变牛膝染色体倍性变异的最佳处理时间,多倍体的特征及其多倍体的细胞学鉴定。结果表明,当秋水... 通过诱变牛膝多倍体,增加新的药用种质资源,为培育牛膝新品系奠定基础。以牛膝(Achyranthes bidentata Blume)为实验材料,研究了秋水仙素的诱变牛膝染色体倍性变异的最佳处理时间,多倍体的特征及其多倍体的细胞学鉴定。结果表明,当秋水仙碱浓度为0.2%时处理最佳处理时间为24 h,诱变率达51.7%。变异株与对照株相比,变异株的生长变缓,叶片增宽变厚,叶色浓绿,叶片皱缩。根尖压片表明,对照株的染色体数为:2n =2x=42,变异株为2n=4x=84。 展开更多
关键词 牛膝 多倍体诱变 改良l.d.cua
下载PDF
金银花多倍体诱变及早期形态鉴定 被引量:10
7
作者 王惠利 赵晓明 《山西农业科学》 2012年第12期1240-1242,1253,共4页
以二倍体金银花(Lonicera japonica Thunb)为材料,采用改良L.D.Cua法对其进行人工诱变,通过对获得的同源四倍体进行植株早期形态鉴定。结果表明,采用改良L.D.Cua法,在药剂浓度0.2%、处理温度20℃、处理时间72 h效果最好,变异率达72.5%;... 以二倍体金银花(Lonicera japonica Thunb)为材料,采用改良L.D.Cua法对其进行人工诱变,通过对获得的同源四倍体进行植株早期形态鉴定。结果表明,采用改良L.D.Cua法,在药剂浓度0.2%、处理温度20℃、处理时间72 h效果最好,变异率达72.5%;金银花染色体加倍后,获得的M1变异植株生长势强,叶片变大变厚,叶色加深,叶形指数变小。 展开更多
关键词 金银花 多倍体诱变育种 改良L D Cua法
下载PDF
杭白芷的多倍体诱变试验
8
作者 王彩霞 《内蒙古林业调查设计》 2017年第5期99-101,共3页
实验通过采用多倍体的化学诱变剂—秋水仙碱来处理杭白芷的幼苗顶芽,对经诱变的材料进行形态学观察和检测分析,并从诱变多倍体的形态学特征(定性)和细胞内部染色体数量的测定(定量)来判断诱变是否成功。结果表明:杭白芷总的诱变成功率为... 实验通过采用多倍体的化学诱变剂—秋水仙碱来处理杭白芷的幼苗顶芽,对经诱变的材料进行形态学观察和检测分析,并从诱变多倍体的形态学特征(定性)和细胞内部染色体数量的测定(定量)来判断诱变是否成功。结果表明:杭白芷总的诱变成功率为78.9%左右。以3d处理为好,诱变率为100%。 展开更多
关键词 杭白芷 多倍体诱变 改良l.d.cua
下载PDF
Appraisal of the Production-Oriented Approach: An Introduction 被引量:4
9
作者 濮实 《Chinese Journal of Applied Linguistics》 SCIE 2017年第4期452-453,共2页
The production-oriented approach (POA) has been developed over a decade. It is driven by the need to improve English classroom instruction for university students in China (Wen, 2016). It is also motivated by the ... The production-oriented approach (POA) has been developed over a decade. It is driven by the need to improve English classroom instruction for university students in China (Wen, 2016). It is also motivated by the aspiration to enhance the quality of foreign language education in other similar pedagogical contexts outside China. A volume of research has been done by Wen Qiufang and her research team, to formulate the theory of POA and to test its effectiveness in classroom pedagogy (e.g. Wen, 2016, 2015; Yang, 2015; Zhang, 2015). At the moment, the POA is still at an early stage of theory building and almost all empirical research is done in the Chinese context. In order to improve the quality of this theory and to make it intelligible to the international academic community, a one-day symposium was held in Beijing Foreign Studies University on May 15, 2017. The symposium was entitled 'The first international forum on innovative foreign language education in China: Appraisal of the POA'. In the forum, leading experts in applied linguistics were invited to discuss the strengths and weaknesses of the POA and the directions for its future development. The symposium was the first attempt for the POA research team to discuss its latest work with international scholars. This Viewpoint section collects the responses of four experts who participated in the symposium, listed in alphabetical order. The collection of articles covers three topics related to the POA: its pedagogical application, its use for teacher training, and its research. Alister Cumming is Professor Emeritus and the former Head of the Centre for Educational Research on Languages and Literacies, University of Toronto, Canada. His article focuses primarily on POA research as an exemplary case of design-based research. Rod Ellis is Research Professor in the School of Education at Curtin University, Australia. He discusses POA in terms of pedagogy, teacher training and research, with both critiques and constructive suggestions. Paul Kei Matsuda is Professor of English and Director of Second Language Writing at Arizona State University, the writed states. He responds to POA from the perspective of an expert researcher and teacher of L2 writing. Charlene Polio is Professor and Associate Chair in the Department of Linguistics & Germanic, Slavic, Asian & African Languages atMichigan State University, the writed states. She conceptualises POA as a useful method to address some issues in pre-service teacher development. Overall, the articles in this section are insightful and reader-friendly. They are not only useful for the development of POA in particular, but may also be valuable to a broad range of researchers as they touch upon pertaining issues, as well as emerging topics, in the field of applied linguistics. We therefore find it necessary to make them accessible to a wide readership. 展开更多
关键词 the production-oriented approach (POA) has been developed over a decade. It is driven by the need to improve English classroom instruction for university students in China (Wen 2016). It is also motivated by the aspiration to enhance the quality of foreign language education in other similar pedagogical contexts outside China. A volume of research has been done by Wen Qiufang and her research team to formulate the theory of POA and to test its effectiveness in classroom pedagogy (e.g. Wen 2016 2015 Yang 2015 Zhang 2015). At the moment the POA is still at an early stage of theory building and almost all empirical research is done in the Chinese context. In order to improve the quality of this theory and to make it intelligible to the international academic community a one-day symposium was held in Beijing Foreign Studies University on May 15 2017. the symposium was entitled the first international forum on innovative foreign language education in China: Appraisal of the POA'. In the forum leading experts in applied linguistics were invited to discuss the strengths and weaknesses of the POA and the directions for its future development. the symposium was the first attempt for the POA research team to discuss its latest work with international scholars. This Viewpoint section collects the responses of four experts who participated in the symposium listed in alphabetical order. the collection of articles covers three topics related to the POA: its pedagogical application its use for teacher training and its research. Alister Cumming is Professor Emeritus and the former Head of the Centre for Educational Research on Languages and Literacies University of Toronto Canada. His article focuses primarily on POA research as an exemplary case of design-based research. Rod Ellis is Research Professor in the School of Education at Curtin University Australia. He discusses POA in terms of pedagogy teacher training and research with both critiques and constructive suggestions. Paul Kei Matsuda is Professor of English and Director of Second Language Writing at Arizona State University the writed states. He responds to POA from the perspective of an expert researcher and teacher of L2 writing. Charlene Polio is Professor and Associate Chair in the Department of Linguistics Germanic Slavic Asian African Languages atMichigan State University the writed states. She conceptualises POA as a useful method to address some issues in pre-service teacher development. Overall the articles in this section are insightful and reader-friendly. they are not only useful for the development of POA in particular but may also be valuable to a broad range of researchers as they touch upon pertaining issues as well as emerging topics in the field of applied linguistics. We therefore find it necessary to make them accessible to a wide readership.
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部