Dissolved organic matter（DOM） represents a significant source of nutrients that supports the microbial-based food web in seagrass ecosystems. However, there is little information on how the various fractions of DOM ...Dissolved organic matter（DOM） represents a significant source of nutrients that supports the microbial-based food web in seagrass ecosystems. However, there is little information on how the various fractions of DOM from seagrass leaves contributed to the coastal biogeochemical cycles. To address this gap, we carried out a 30-day laboratory chamber experiment on tropical seagrasses Thalassia hemprichii and Enhalus acoroides. After 30 days of incubation, on average 22% carbon（C）, 70% nitrogen（N） and 38% phosphorus（P） of these two species of seagrass leaf litter was released. The average leached dissolved organic carbon（DOC）, dissolved organic nitrogen（DON） and dissolved organic phosphorus（DOP） of these two species of seagrass leaf litter accounted for 55%, 95% and 65% of the total C, N and P lost, respectively. In the absence of microbes, about 75% of the total amount of DOC, monosaccharides（MCHO）, DON and DOP were quickly released via leaching from both seagrass species in the first 9 days. Subsequently, little DOM was released during the remainder of the experiment. The leaching rates of DOC, DON and DOP were approximately 110, 40 and 0.70 μmol/（g·d）. Leaching rates of DOM were attributed to the nonstructural carbohydrates and other labile organic matter within the seagrass leaf. Thalassia hemprichii leached more DOC, DOP and MCHO than E. acoroides. In contrast, E. acoroides leached higher concentrations of DON than T. hemprichii, with the overall leachate also having a higher DON： DOP ratio. These results indicate that there is an overall higher amount of DOM leachate from T. hemprichii than that of E. acoroides that is available to the seagrass ecosystem. According to the logarithmic model for DOM release and the in situ leaf litter production（the Xincun Bay, South China Sea）, the seagrass leaf litter of these two seagrass species could release approximately 4×10~3 mol/d DOC, 1.4×10~3 mol/d DON and 25 mol/d DOP into the seawater. In addition to providing readily available nutrients for the microbial food web, the remaining particulate organic matter（POM）from the litter would also enter microbial remineralization processes. What is not remineralized from either DOM or POM fractions has potential to contribute to the permanent carbon stocks.展开更多
Olive productivity should be improved through stimulating nutrition,particularly under poor fertility soils.Consequently,the objective of this study was to assess the efficacy of applying organic and bio-fertilizers o...Olive productivity should be improved through stimulating nutrition,particularly under poor fertility soils.Consequently,the objective of this study was to assess the efficacy of applying organic and bio-fertilizers on the physiological growth,yield and fruit quality of olive trees under newly reclaimed poor-fertility sandy soil in an arid environment.During a field experiment carried out at El-Qantara,North Sinai,Egypt over two consecutive seasons(2019-2020 and 2020-2021),olive Kalamata trees were evaluated under three organic fertilizer treatments alone or in combination with three bio-fertilizers treatments.Organic fertilizer was applied as goat manure(16.8 kg/tree/year),or olive pomace(8.5 kg/tree/year)in mid-December of each season vs.untreated trees.The bio-fertilizers were applied as N-fixing bacteria(150 g/tree)was inculated in early March of each season,or amino acid mixture(1.5%)was applied three times,at 70%of full bloom,21 days after full bloom,and a month later in comparison to a non-fertilized trees(control).The cultivar used was Kalamata,a dual-purpose cultivar for oil and table olives whose value increases when processed as table olives.The results indicated that the goat manure followed by olive pomace significantly enhanced photosynthetic pigments(chlorophyll a,b,and carotenoids),leaf mineral contents(N,P,K,Ca,Mg and Fe),tree canopy volume,number of flowers per inflorescence,number of inflorescences per shoot,initial fruit set,fruit retention.For fruit quality,fruit length and width,fruit weight,and total fruit yield was increased compared to the non-fertilized control.Likewise,The bio-fertilizer N-fixing bacteria followed by the amino acid mixture significantly improved all of the aforementioned parameters.Accordingly,it is recommended,both environmentally and economically to utilize organic and bio-fertizers,particularly goat manure combined with N-fixing bacteria,in low-fertility soil to sustain olive production as well as reducing mineral fertilization.展开更多
The objective of this study was to determine the impact of frequency of broad-leaf crops canola and pea in various crop rotations on pH, total organic C (TOC), total organic N (TON), light fraction organic C (LFOC) an...The objective of this study was to determine the impact of frequency of broad-leaf crops canola and pea in various crop rotations on pH, total organic C (TOC), total organic N (TON), light fraction organic C (LFOC) and light fraction organic N (LFON) in the 0 - 7.5 and 7.5 - 15 cm soil depths in autumn 2009 after 12 years (1998-2009) on a Dark Brown Chernozem (Typic Boroll) loam at Scott, Saskatchewan, Canada. The field ex-periment contained monoculture canola (herbicide tolerant and blackleg resistant hybrid) and monoculture pea compared with rotations that contained these crops every 2-, 3-, and 4-yr with wheat. There was no effect of crop rotation duration and crop phase on soil pH. Mass of TOC and TON in the 0 - 15 cm soil was greater in canola phase than pea phase in the 1-yr (monoculture) and 2-yr crop rotations, while the opposite was true in the 3-yr and 4-yr crop rotations. Mass of TOC and TON (averaged across crop phases,) in soil generally increased with increasing crop rotation duration, with the maximum in the 4-yr rotation while no difference in the 1-yr and 2-yr rotations. Mass of LFOC and LFON in soil was greater in canola phase than pea phase in the 1-yr, 2-yr and 3-yr rotations, but the opposite was true in the 4-yr rotation. There was no consistent effect of crop rotation duration on mass of LFOC and LFON. The N balance sheet over the 1998 to 2009 period indicated large amounts of unaccounted N for monoculture pea, suggesting a great potential for N loss from the soil-plant system in this treatment through nitrate leaching and/or denitrification. In conclusion, the findings suggest that the quantity of organic C and N can be maximized by increasing duration of crop rotation and by including hybrid canola in the rotation.展开更多
植物顶端分生组织可分为中央区,周缘区和肋区。在植物胚后发育中,侧生器官产生于顶端分生组织的周缘区。顶端分生组织和侧生器官之间的边界的建立和维持是一个非常重要的发育过程,许多调节子参与控制这个过程。拟南芥的 LATERALORGANBOU...植物顶端分生组织可分为中央区,周缘区和肋区。在植物胚后发育中,侧生器官产生于顶端分生组织的周缘区。顶端分生组织和侧生器官之间的边界的建立和维持是一个非常重要的发育过程,许多调节子参与控制这个过程。拟南芥的 LATERALORGANBOUNDARIES(LOB)基因具有独特的表达模式,其表达的范围与上述的边界区域重合。LOB基因隶属于一个大的基因家族——L O B 结构域基因家族。该家族编码的蛋白在 N 端具有一个保守的LOB结构域,该家族 LOB 基因以外的成员也参与拟南芥不同的发育过程。为了探讨在与拟南芥亲缘关系较远的豆科中 LOB 同源基因的功能,我们在豆科模式植物百脉根中分离了3 个 LOB 同源基因,命名为 LjLOB 基因,并用 RNA 原位杂交方法研究了这3个基因的表达模式。研究结果显示,L j L O B 1 和LjLOB3都强烈地在小叶原基的基部表达,这种表达模式可能与小叶原基和复叶原基之间的边界相关。而LjLOB4则在发育中的花芽不同轮之间的边界上表达。百脉根中这3 个基因具有不同的表达模式,强烈地提示它们的功能发生了分歧:L j L O B 1 和L j L O B 3 可能在复叶发育中具有重要功能;而LjLOB4 则可能参与了花的发育。展开更多
基金The National Basic Research Program of China under contract Nos 2015CB452905 and 2015CB452902the National Natural Science Foundation of China under contract No.41730529the National Specialized Project of Science and Technology under contract No.2015FY110600
文摘Dissolved organic matter（DOM） represents a significant source of nutrients that supports the microbial-based food web in seagrass ecosystems. However, there is little information on how the various fractions of DOM from seagrass leaves contributed to the coastal biogeochemical cycles. To address this gap, we carried out a 30-day laboratory chamber experiment on tropical seagrasses Thalassia hemprichii and Enhalus acoroides. After 30 days of incubation, on average 22% carbon（C）, 70% nitrogen（N） and 38% phosphorus（P） of these two species of seagrass leaf litter was released. The average leached dissolved organic carbon（DOC）, dissolved organic nitrogen（DON） and dissolved organic phosphorus（DOP） of these two species of seagrass leaf litter accounted for 55%, 95% and 65% of the total C, N and P lost, respectively. In the absence of microbes, about 75% of the total amount of DOC, monosaccharides（MCHO）, DON and DOP were quickly released via leaching from both seagrass species in the first 9 days. Subsequently, little DOM was released during the remainder of the experiment. The leaching rates of DOC, DON and DOP were approximately 110, 40 and 0.70 μmol/（g·d）. Leaching rates of DOM were attributed to the nonstructural carbohydrates and other labile organic matter within the seagrass leaf. Thalassia hemprichii leached more DOC, DOP and MCHO than E. acoroides. In contrast, E. acoroides leached higher concentrations of DON than T. hemprichii, with the overall leachate also having a higher DON： DOP ratio. These results indicate that there is an overall higher amount of DOM leachate from T. hemprichii than that of E. acoroides that is available to the seagrass ecosystem. According to the logarithmic model for DOM release and the in situ leaf litter production（the Xincun Bay, South China Sea）, the seagrass leaf litter of these two seagrass species could release approximately 4×10~3 mol/d DOC, 1.4×10~3 mol/d DON and 25 mol/d DOP into the seawater. In addition to providing readily available nutrients for the microbial food web, the remaining particulate organic matter（POM）from the litter would also enter microbial remineralization processes. What is not remineralized from either DOM or POM fractions has potential to contribute to the permanent carbon stocks.
基金The authors extend their appreciation to the Deanship of Scientific Research at Jouf University for funding this work through Research Grant No.(DSR2020-01-2553).
文摘Olive productivity should be improved through stimulating nutrition,particularly under poor fertility soils.Consequently,the objective of this study was to assess the efficacy of applying organic and bio-fertilizers on the physiological growth,yield and fruit quality of olive trees under newly reclaimed poor-fertility sandy soil in an arid environment.During a field experiment carried out at El-Qantara,North Sinai,Egypt over two consecutive seasons(2019-2020 and 2020-2021),olive Kalamata trees were evaluated under three organic fertilizer treatments alone or in combination with three bio-fertilizers treatments.Organic fertilizer was applied as goat manure(16.8 kg/tree/year),or olive pomace(8.5 kg/tree/year)in mid-December of each season vs.untreated trees.The bio-fertilizers were applied as N-fixing bacteria(150 g/tree)was inculated in early March of each season,or amino acid mixture(1.5%)was applied three times,at 70%of full bloom,21 days after full bloom,and a month later in comparison to a non-fertilized trees(control).The cultivar used was Kalamata,a dual-purpose cultivar for oil and table olives whose value increases when processed as table olives.The results indicated that the goat manure followed by olive pomace significantly enhanced photosynthetic pigments(chlorophyll a,b,and carotenoids),leaf mineral contents(N,P,K,Ca,Mg and Fe),tree canopy volume,number of flowers per inflorescence,number of inflorescences per shoot,initial fruit set,fruit retention.For fruit quality,fruit length and width,fruit weight,and total fruit yield was increased compared to the non-fertilized control.Likewise,The bio-fertilizer N-fixing bacteria followed by the amino acid mixture significantly improved all of the aforementioned parameters.Accordingly,it is recommended,both environmentally and economically to utilize organic and bio-fertizers,particularly goat manure combined with N-fixing bacteria,in low-fertility soil to sustain olive production as well as reducing mineral fertilization.
文摘The objective of this study was to determine the impact of frequency of broad-leaf crops canola and pea in various crop rotations on pH, total organic C (TOC), total organic N (TON), light fraction organic C (LFOC) and light fraction organic N (LFON) in the 0 - 7.5 and 7.5 - 15 cm soil depths in autumn 2009 after 12 years (1998-2009) on a Dark Brown Chernozem (Typic Boroll) loam at Scott, Saskatchewan, Canada. The field ex-periment contained monoculture canola (herbicide tolerant and blackleg resistant hybrid) and monoculture pea compared with rotations that contained these crops every 2-, 3-, and 4-yr with wheat. There was no effect of crop rotation duration and crop phase on soil pH. Mass of TOC and TON in the 0 - 15 cm soil was greater in canola phase than pea phase in the 1-yr (monoculture) and 2-yr crop rotations, while the opposite was true in the 3-yr and 4-yr crop rotations. Mass of TOC and TON (averaged across crop phases,) in soil generally increased with increasing crop rotation duration, with the maximum in the 4-yr rotation while no difference in the 1-yr and 2-yr rotations. Mass of LFOC and LFON in soil was greater in canola phase than pea phase in the 1-yr, 2-yr and 3-yr rotations, but the opposite was true in the 4-yr rotation. There was no consistent effect of crop rotation duration on mass of LFOC and LFON. The N balance sheet over the 1998 to 2009 period indicated large amounts of unaccounted N for monoculture pea, suggesting a great potential for N loss from the soil-plant system in this treatment through nitrate leaching and/or denitrification. In conclusion, the findings suggest that the quantity of organic C and N can be maximized by increasing duration of crop rotation and by including hybrid canola in the rotation.
文摘植物顶端分生组织可分为中央区,周缘区和肋区。在植物胚后发育中,侧生器官产生于顶端分生组织的周缘区。顶端分生组织和侧生器官之间的边界的建立和维持是一个非常重要的发育过程,许多调节子参与控制这个过程。拟南芥的 LATERALORGANBOUNDARIES(LOB)基因具有独特的表达模式,其表达的范围与上述的边界区域重合。LOB基因隶属于一个大的基因家族——L O B 结构域基因家族。该家族编码的蛋白在 N 端具有一个保守的LOB结构域,该家族 LOB 基因以外的成员也参与拟南芥不同的发育过程。为了探讨在与拟南芥亲缘关系较远的豆科中 LOB 同源基因的功能,我们在豆科模式植物百脉根中分离了3 个 LOB 同源基因,命名为 LjLOB 基因,并用 RNA 原位杂交方法研究了这3个基因的表达模式。研究结果显示,L j L O B 1 和LjLOB3都强烈地在小叶原基的基部表达,这种表达模式可能与小叶原基和复叶原基之间的边界相关。而LjLOB4则在发育中的花芽不同轮之间的边界上表达。百脉根中这3 个基因具有不同的表达模式,强烈地提示它们的功能发生了分歧:L j L O B 1 和L j L O B 3 可能在复叶发育中具有重要功能;而LjLOB4 则可能参与了花的发育。
