泰国影片《The Love of Siam》以自身真纯质朴的格调赢得过诸多赞誉。2012年,片中两位主演再次在中国国内掀起关注热潮。阅览他们的过往和现在,可以轻易地联系起诸多类似的人和事。随后进行的看似随意的拼贴,实际上却能折射出颇为深致...泰国影片《The Love of Siam》以自身真纯质朴的格调赢得过诸多赞誉。2012年,片中两位主演再次在中国国内掀起关注热潮。阅览他们的过往和现在,可以轻易地联系起诸多类似的人和事。随后进行的看似随意的拼贴,实际上却能折射出颇为深致的命理:世易时移,有爱足矣!千百年来,它依旧卓然自立,无关性别、无关年龄,甚至无关生死。展开更多
In this essay, I will explore human being's aspiration for love conveyed in the novel A Haunted House by Virginia Woolf. The exploration starts from the analysis of the ghostly couple's horror for death, the l...In this essay, I will explore human being's aspiration for love conveyed in the novel A Haunted House by Virginia Woolf. The exploration starts from the analysis of the ghostly couple's horror for death, the living couples dreaming of the ghostly cou ples' finding of the treasure of love, and finally to the description of the loving scenes of this couple.展开更多
Frederic Henry,the hero in Ernest Hemingway's novel A Farewell to Arms,demonstrates the author's dominant motif of a man who is forced to recognize the inevitability of death and the futility of trying to esca...Frederic Henry,the hero in Ernest Hemingway's novel A Farewell to Arms,demonstrates the author's dominant motif of a man who is forced to recognize the inevitability of death and the futility of trying to escape from it.This essay divides the development of Frederic Henry's death consciousness into three stages and analyzes his psychological features of each stage.展开更多
The essay is on the novel of Jane Eyre written by Charlotte Bronte.Jane was an orphan,finally she developed into an intellectual,confident and independent woman.She married her true love and realized her dream at last...The essay is on the novel of Jane Eyre written by Charlotte Bronte.Jane was an orphan,finally she developed into an intellectual,confident and independent woman.She married her true love and realized her dream at last.During the period of her growth,many deaths appeared in Jane's life.These deaths play an important part in the novel.This essay will discuss the function of death in the novel.The author's view of death was expressed through Jane's life,love and her character.Through the arguments in the novel,we can draw a conclusion:death is not fearful.Love is eternal.We should learn how to love-love everything around us and cherish everything that you own.展开更多
爱与死亡一直是人类不断思索的问题。英国著名作家朱利安·巴恩斯的小说《生命的层级》(Levels of Life)中集中探讨了爱和死亡的话题,折射出当代人生存中的困境,延续了其对爱情、存在和对终极的叩问。巴恩斯旨在告诫人们要学会直面...爱与死亡一直是人类不断思索的问题。英国著名作家朱利安·巴恩斯的小说《生命的层级》(Levels of Life)中集中探讨了爱和死亡的话题,折射出当代人生存中的困境,延续了其对爱情、存在和对终极的叩问。巴恩斯旨在告诫人们要学会直面爱与死亡,通过文字表面的情感刻画,引发人们对于爱和死亡本身所具有的历史和道德意义的思考,理解个体存在的意义与价值。展开更多
Infections of many viruses induce caspase activation to regulate multiple cellular pathways,including programmed cell death,immune signaling and etc.Characterizations of caspase cleavage sites and substrates are impor...Infections of many viruses induce caspase activation to regulate multiple cellular pathways,including programmed cell death,immune signaling and etc.Characterizations of caspase cleavage sites and substrates are important for understanding the regulation mechanisms of caspase activation.Here,we identified and analyzed a novel caspase cleavage motif AEAD,and confirmed its caspase dependent cleavage activity in natural substrate,such as nitric oxide-associated protein 1(NOA1).Fusing the enhanced green fluorescent protein(EGFP)with the mitochondrial marker protein Tom20 through the AEAD motif peptide localized EGFP to the mitochondria.Upon the activation of caspase triggered by Sendai virus(SeV)or herpes simplex virus type 1(HSV-1)infection,EGFP diffusely localized to the cell due to the caspase-mediated cleavage,thus allowing visual detection of the virusinduced caspase activation.An AEAD peptide-derived inhibitor Z-AEAD-FMK were developed,which significantly inhibited the activities of caspases-1,-3,-6,-7,-8 and-9,exhibiting a broad caspase inhibition effect.The inhibitor further prevented caspases-mediated cleavage of downstream substrates,including BID,PARP1,LMNA,pro-IL-1β,pro-IL-18,GSDMD and GSDME,protecting cells from virus-induced apoptotic and pyroptotic cell death.Together,our findings provide a new perspective for the identification of novel caspase cleavage motifs and the development of new caspase inhibitors and anti-inflammatory drugs.展开更多
文摘泰国影片《The Love of Siam》以自身真纯质朴的格调赢得过诸多赞誉。2012年,片中两位主演再次在中国国内掀起关注热潮。阅览他们的过往和现在,可以轻易地联系起诸多类似的人和事。随后进行的看似随意的拼贴,实际上却能折射出颇为深致的命理:世易时移,有爱足矣!千百年来,它依旧卓然自立,无关性别、无关年龄,甚至无关生死。
文摘In this essay, I will explore human being's aspiration for love conveyed in the novel A Haunted House by Virginia Woolf. The exploration starts from the analysis of the ghostly couple's horror for death, the living couples dreaming of the ghostly cou ples' finding of the treasure of love, and finally to the description of the loving scenes of this couple.
文摘Frederic Henry,the hero in Ernest Hemingway's novel A Farewell to Arms,demonstrates the author's dominant motif of a man who is forced to recognize the inevitability of death and the futility of trying to escape from it.This essay divides the development of Frederic Henry's death consciousness into three stages and analyzes his psychological features of each stage.
文摘The essay is on the novel of Jane Eyre written by Charlotte Bronte.Jane was an orphan,finally she developed into an intellectual,confident and independent woman.She married her true love and realized her dream at last.During the period of her growth,many deaths appeared in Jane's life.These deaths play an important part in the novel.This essay will discuss the function of death in the novel.The author's view of death was expressed through Jane's life,love and her character.Through the arguments in the novel,we can draw a conclusion:death is not fearful.Love is eternal.We should learn how to love-love everything around us and cherish everything that you own.
文摘爱与死亡一直是人类不断思索的问题。英国著名作家朱利安·巴恩斯的小说《生命的层级》(Levels of Life)中集中探讨了爱和死亡的话题,折射出当代人生存中的困境,延续了其对爱情、存在和对终极的叩问。巴恩斯旨在告诫人们要学会直面爱与死亡,通过文字表面的情感刻画,引发人们对于爱和死亡本身所具有的历史和道德意义的思考,理解个体存在的意义与价值。
基金supported by the National Key R&D Program of China(2021YFC2300700)National Science and Technology Major Project(No.2018ZX10101004001005)National Natural Science Foundation of China(numbers 32070179).We thank Dr.Qinxue Hu(Wuhan Institute of Virology)and Dr.Yuchen Xia(Wuhan University)for help with materials.We thank Ding Gao from Center for Instrumental Analysis and Metrology at Wuhan Institute of Virology for his help with the Leica confocal microscope and the Operetta.
文摘Infections of many viruses induce caspase activation to regulate multiple cellular pathways,including programmed cell death,immune signaling and etc.Characterizations of caspase cleavage sites and substrates are important for understanding the regulation mechanisms of caspase activation.Here,we identified and analyzed a novel caspase cleavage motif AEAD,and confirmed its caspase dependent cleavage activity in natural substrate,such as nitric oxide-associated protein 1(NOA1).Fusing the enhanced green fluorescent protein(EGFP)with the mitochondrial marker protein Tom20 through the AEAD motif peptide localized EGFP to the mitochondria.Upon the activation of caspase triggered by Sendai virus(SeV)or herpes simplex virus type 1(HSV-1)infection,EGFP diffusely localized to the cell due to the caspase-mediated cleavage,thus allowing visual detection of the virusinduced caspase activation.An AEAD peptide-derived inhibitor Z-AEAD-FMK were developed,which significantly inhibited the activities of caspases-1,-3,-6,-7,-8 and-9,exhibiting a broad caspase inhibition effect.The inhibitor further prevented caspases-mediated cleavage of downstream substrates,including BID,PARP1,LMNA,pro-IL-1β,pro-IL-18,GSDMD and GSDME,protecting cells from virus-induced apoptotic and pyroptotic cell death.Together,our findings provide a new perspective for the identification of novel caspase cleavage motifs and the development of new caspase inhibitors and anti-inflammatory drugs.
