In 18th century European arts were greatly influenced by eastern Asia while Asian soci-ety was also immersed in "Europeanixation" and affected by economic life of industrialworld.Therefore it came the worldw...In 18th century European arts were greatly influenced by eastern Asia while Asian soci-ety was also immersed in "Europeanixation" and affected by economic life of industrialworld.Therefore it came the worldwide exchange.The oriental arts were widely abserbedby the art of Rococo.The reasons lay in some similaritse between these two kinds of arts.The devclopment of Rococo was advanced by means of static observation and technique ofexpressions of mobile change of oriental arts. why has the style of Rococo been in vogueonce again these years? It is the reflection of deep social ideological trend.展开更多
Maximizing the power capture is an important issue to the turbines that are installed in low wind speed area. In this paper, we focused on the modeling and control of variable speed wind turbine that is composed of tw...Maximizing the power capture is an important issue to the turbines that are installed in low wind speed area. In this paper, we focused on the modeling and control of variable speed wind turbine that is composed of two-mass drive train, a Squirrel Cage Induction Generator (SCIG), and voltage source converter control by Space Vector Pulse Width Modulation (SPVWM). To achieve Maximum Power Point Tracking (MPPT), the reference speed to the generator is searched via Extremum Seeking Control (ESC). ESC was designed for wind turbine region II operation based on dither-modulation scheme. ESC is a model-free method that has the ability to increase the captured power in real time under turbulent wind without any requirement for wind measurements. The controller is designed in two loops. In the outer loop, ESC is used to set a desired reference speed to PI controller to regulate the speed of the generator and extract the maximum electrical power. The inner control loop is based on Indirect Field Orientation Control (IFOC) to decouple the currents. Finally, Particle Swarm Optimization (PSO) is used to obtain the optimal PI parameters. Simulation and control of the system have been accomplished using MATLAB/Simulink 2014.展开更多
Oriented immobilization of enzymes helps to maintain their native structure and proper orientation for high-performance engineering to meet extensive biocatalysis demands.However,the supporting materials used for orie...Oriented immobilization of enzymes helps to maintain their native structure and proper orientation for high-performance engineering to meet extensive biocatalysis demands.However,the supporting materials used for orientated immobilization are usually costly or complicated in preparation,affecting their practical applications.In this work,a facile purification and immobilization method was proposed for enzyme immobilization based on organic-inorganic hybrid calcium phosphate nanocrystal(Ca Ps)induced by Cu^(2+) modified bovine serum albumin(BSA-Cu).Then,the as-prepared hybrid calcium phosphate nanosheet,BSA-Cu@Ca Ps,was utilized for one-pot purification and immobilization of His-tagged organophosphorus hydrolase(OPH)by metal-affinity binding to the incorporated BSA.BSA-Cu@Ca PsOPH exhibited enhanced p H stability and thermal stability compared to the free enzyme.Moreover,BSA-Cu@Ca Ps-OPH could retain more than 75%and 56%of initial activity after reuse 5 and 10 times,respectively.The results demonstrated that this facile strategy was promising for the effective biodegradation of organophosphorus pesticides with the immobilized enzyme.展开更多
文摘In 18th century European arts were greatly influenced by eastern Asia while Asian soci-ety was also immersed in "Europeanixation" and affected by economic life of industrialworld.Therefore it came the worldwide exchange.The oriental arts were widely abserbedby the art of Rococo.The reasons lay in some similaritse between these two kinds of arts.The devclopment of Rococo was advanced by means of static observation and technique ofexpressions of mobile change of oriental arts. why has the style of Rococo been in vogueonce again these years? It is the reflection of deep social ideological trend.
文摘Maximizing the power capture is an important issue to the turbines that are installed in low wind speed area. In this paper, we focused on the modeling and control of variable speed wind turbine that is composed of two-mass drive train, a Squirrel Cage Induction Generator (SCIG), and voltage source converter control by Space Vector Pulse Width Modulation (SPVWM). To achieve Maximum Power Point Tracking (MPPT), the reference speed to the generator is searched via Extremum Seeking Control (ESC). ESC was designed for wind turbine region II operation based on dither-modulation scheme. ESC is a model-free method that has the ability to increase the captured power in real time under turbulent wind without any requirement for wind measurements. The controller is designed in two loops. In the outer loop, ESC is used to set a desired reference speed to PI controller to regulate the speed of the generator and extract the maximum electrical power. The inner control loop is based on Indirect Field Orientation Control (IFOC) to decouple the currents. Finally, Particle Swarm Optimization (PSO) is used to obtain the optimal PI parameters. Simulation and control of the system have been accomplished using MATLAB/Simulink 2014.
基金supported by the National Key Research and Development Program of China(2021YFC2102801)the National Natural Science Foundation of China(21621004)。
文摘Oriented immobilization of enzymes helps to maintain their native structure and proper orientation for high-performance engineering to meet extensive biocatalysis demands.However,the supporting materials used for orientated immobilization are usually costly or complicated in preparation,affecting their practical applications.In this work,a facile purification and immobilization method was proposed for enzyme immobilization based on organic-inorganic hybrid calcium phosphate nanocrystal(Ca Ps)induced by Cu^(2+) modified bovine serum albumin(BSA-Cu).Then,the as-prepared hybrid calcium phosphate nanosheet,BSA-Cu@Ca Ps,was utilized for one-pot purification and immobilization of His-tagged organophosphorus hydrolase(OPH)by metal-affinity binding to the incorporated BSA.BSA-Cu@Ca PsOPH exhibited enhanced p H stability and thermal stability compared to the free enzyme.Moreover,BSA-Cu@Ca Ps-OPH could retain more than 75%and 56%of initial activity after reuse 5 and 10 times,respectively.The results demonstrated that this facile strategy was promising for the effective biodegradation of organophosphorus pesticides with the immobilized enzyme.
