This research aims at developing a plant regeneration system from leaf and petiole explants of Anthurium andraeanum Hort., thereby establish a foundation for mass production and transformation. Using tissue culture te...This research aims at developing a plant regeneration system from leaf and petiole explants of Anthurium andraeanum Hort., thereby establish a foundation for mass production and transformation. Using tissue culture technique, the conditions for callus induction, protocorm-like body (PLB) formation and plant regeneration from leaf explants and petiole of A. andraeanum, such as basal medium and plant growth regulator, were investigated. Totipotent callus was induced on a 1/2-strength MS medium containing 0.90 μmol L^-1 2,4-dichlorophenoxyacetic acid (2,4-D) and 8.88μmol L^-1 N6-benzyladenine (BA). The callus exhibited complete hormone autonomy for growth and differentiation of PLBs. This callus proliferated well and was maintained by subculturing on 1/2 MS medium containing 0.90 μmol L^-1 2,4-D and 4.44 μmol L^-1 BA. On average, 8 protocorm-like bodies could be obtained from a piece of 4 mm callus after being transferred to the 1/2 MS medium with 4.44 μmol L^-1 BA after 8 wk of culture. The regenerated PLBs formed shoots and roots on 1/2 MS medium. After 24 wk of culture on these medium, well-developed plantlets for potting were produced. An efficient micropropagation method was established for indirect PLB formation and plant regeneration from leaf and petiole ofA. andraeanum.展开更多
The Binchuan region of Yunnan is a structurally complex region with mountains,basins,and active faults.In this situation,seismic wave propagation exhibits complex characteristics due to strong heterogeneity of undergr...The Binchuan region of Yunnan is a structurally complex region with mountains,basins,and active faults.In this situation,seismic wave propagation exhibits complex characteristics due to strong heterogeneity of underground media instead of following the great-circle path.In order to obtain a high-resolution shallow crustal structure,a dense seismic array was deployed during March 21 to May 30,2017 in this area.To better understand the complexities of seismic wave propagation in this region,we perform array-based frequency-domain beamforming analysis and single-station based polarization analysis to investigate the characteristics of seismic wave propagation,using airgun-generated P-wave signals recorded by dense array stations in this experiment.The results from these two methods both reveal similar but complex characteristics of seismic wave propagation in the Binchuan basin.The azimuth anomalies off the great-circle path are quite large with values up to 30°,which is caused by strong structural heterogeneity in the very shallow crust.Our research provide a better understanding of the complex geologic structures in this area and provide guidance for detecting concealed faults and distribution of velocity anomalies.展开更多
Human body communication(HBC) is a promising near-field communication(NFC) method emerging in recent years. But existing theoretical models of HBC are too simple to simulate the wave propagation on human body. In this...Human body communication(HBC) is a promising near-field communication(NFC) method emerging in recent years. But existing theoretical models of HBC are too simple to simulate the wave propagation on human body. In this work, in order to clarify the propagation mechanism of electromagnetic wave on human body, a surface waveguide HBC theoretical model based on stratified media cylinder is presented. A numerical model analyzed by finite element method(FEM) is used for comparing and validating the theoretical model. Finally, results of theoretical and numerical models from 80 MHz to 200 MHz agree fairly well, which means that theoretical model can characterize accurate propagation mechanism of HBC signal. Meanwhile, attenuation constants derived from two kinds of models are within the range from 1.64 to 3.37, so that HBC signal can propagate effectively on human body. The propagation mechanism derived from the theoretical model is useful to provide design information for the transmitter and the modeling of the propagation channel in HBC.展开更多
利用裂纹扩展分析方法和质量分类法研究铝合金车体含缺欠焊接结构的疲劳寿命问题。首先,研究IIW:2015的基于Paris定律的裂纹扩展分析的理论基础和BS 7910:2019的基于考虑焊接缺欠质量的S-N曲线的质量分类法的算法原理;其次,归纳总结了这...利用裂纹扩展分析方法和质量分类法研究铝合金车体含缺欠焊接结构的疲劳寿命问题。首先,研究IIW:2015的基于Paris定律的裂纹扩展分析的理论基础和BS 7910:2019的基于考虑焊接缺欠质量的S-N曲线的质量分类法的算法原理;其次,归纳总结了这2种方法进行考虑焊接质量的焊接结构疲劳寿命评估流程;再次,在疲劳载荷作用下,基于BS EN 1999-1-3:2007的名义应力法对某动车组铝合金车体进行疲劳分析,确定车体疲劳关切焊缝部位;最后,分别利用裂纹扩展分析方法和质量分类法对车体关切焊缝侧门下角进行考虑初始裂纹的疲劳寿命评估。结果表明:基于质量分类法的剩余寿命为1.4×106次,基于裂纹扩展分析法的剩余寿命为2.64×106次。展开更多
α-Synuclein is a protein that mainly exists in the presynaptic terminals.Abnormal folding and accumulation of α-synuclein are found in several neurodegenerative diseases,including Parkinson’s disease.Aggregated and...α-Synuclein is a protein that mainly exists in the presynaptic terminals.Abnormal folding and accumulation of α-synuclein are found in several neurodegenerative diseases,including Parkinson’s disease.Aggregated and highly phospho rylated a-synuclein constitutes the main component of Lewy bodies in the brain,the pathological hallmark of Parkinson s disease.For decades,much attention has been focused on the accumulation of α-synuclein in the brain parenchyma rather than considering Parkinson s disease as a systemic disease.Recent evidence demonstrates that,at least in some patients,the initial α-synuclein pathology originates in the peripheral organs and spreads to the brain.Injection of α-synuclein preformed fibrils into the gastrointestinal tra ct trigge rs the gutto-brain propagation of α-synuclein pathology.However,whether α-synuclein pathology can occur spontaneously in peripheral organs independent of exogenous α-synuclein preformed fibrils or pathological α-synuclein leakage from the central nervous system remains under investigation.In this review,we aimed to summarize the role of peripheral α-synuclein pathology in the pathogenesis of Parkinson’s disease.We also discuss the pathways by which α-synuclein pathology spreads from the body to the brain.展开更多
基金supported by the Natural Science Foundation of Guangdong Province (05300848)Fok Ying Tung Education Foundation (104031)the National Natural Science Foundation of China(30800758)
文摘This research aims at developing a plant regeneration system from leaf and petiole explants of Anthurium andraeanum Hort., thereby establish a foundation for mass production and transformation. Using tissue culture technique, the conditions for callus induction, protocorm-like body (PLB) formation and plant regeneration from leaf explants and petiole of A. andraeanum, such as basal medium and plant growth regulator, were investigated. Totipotent callus was induced on a 1/2-strength MS medium containing 0.90 μmol L^-1 2,4-dichlorophenoxyacetic acid (2,4-D) and 8.88μmol L^-1 N6-benzyladenine (BA). The callus exhibited complete hormone autonomy for growth and differentiation of PLBs. This callus proliferated well and was maintained by subculturing on 1/2 MS medium containing 0.90 μmol L^-1 2,4-D and 4.44 μmol L^-1 BA. On average, 8 protocorm-like bodies could be obtained from a piece of 4 mm callus after being transferred to the 1/2 MS medium with 4.44 μmol L^-1 BA after 8 wk of culture. The regenerated PLBs formed shoots and roots on 1/2 MS medium. After 24 wk of culture on these medium, well-developed plantlets for potting were produced. An efficient micropropagation method was established for indirect PLB formation and plant regeneration from leaf and petiole ofA. andraeanum.
基金sponsored by the National Natural Science Foundation of China(GG2080000476)the China Earthquake Science Experiment Project,China Earthquake Administration(2017CESE0101,2018CSES0101)
文摘The Binchuan region of Yunnan is a structurally complex region with mountains,basins,and active faults.In this situation,seismic wave propagation exhibits complex characteristics due to strong heterogeneity of underground media instead of following the great-circle path.In order to obtain a high-resolution shallow crustal structure,a dense seismic array was deployed during March 21 to May 30,2017 in this area.To better understand the complexities of seismic wave propagation in this region,we perform array-based frequency-domain beamforming analysis and single-station based polarization analysis to investigate the characteristics of seismic wave propagation,using airgun-generated P-wave signals recorded by dense array stations in this experiment.The results from these two methods both reveal similar but complex characteristics of seismic wave propagation in the Binchuan basin.The azimuth anomalies off the great-circle path are quite large with values up to 30°,which is caused by strong structural heterogeneity in the very shallow crust.Our research provide a better understanding of the complex geologic structures in this area and provide guidance for detecting concealed faults and distribution of velocity anomalies.
基金Project(2009ZX01031-001-007-2)supported by the National Science and Technology Major Project,China
文摘Human body communication(HBC) is a promising near-field communication(NFC) method emerging in recent years. But existing theoretical models of HBC are too simple to simulate the wave propagation on human body. In this work, in order to clarify the propagation mechanism of electromagnetic wave on human body, a surface waveguide HBC theoretical model based on stratified media cylinder is presented. A numerical model analyzed by finite element method(FEM) is used for comparing and validating the theoretical model. Finally, results of theoretical and numerical models from 80 MHz to 200 MHz agree fairly well, which means that theoretical model can characterize accurate propagation mechanism of HBC signal. Meanwhile, attenuation constants derived from two kinds of models are within the range from 1.64 to 3.37, so that HBC signal can propagate effectively on human body. The propagation mechanism derived from the theoretical model is useful to provide design information for the transmitter and the modeling of the propagation channel in HBC.
文摘利用裂纹扩展分析方法和质量分类法研究铝合金车体含缺欠焊接结构的疲劳寿命问题。首先,研究IIW:2015的基于Paris定律的裂纹扩展分析的理论基础和BS 7910:2019的基于考虑焊接缺欠质量的S-N曲线的质量分类法的算法原理;其次,归纳总结了这2种方法进行考虑焊接质量的焊接结构疲劳寿命评估流程;再次,在疲劳载荷作用下,基于BS EN 1999-1-3:2007的名义应力法对某动车组铝合金车体进行疲劳分析,确定车体疲劳关切焊缝部位;最后,分别利用裂纹扩展分析方法和质量分类法对车体关切焊缝侧门下角进行考虑初始裂纹的疲劳寿命评估。结果表明:基于质量分类法的剩余寿命为1.4×106次,基于裂纹扩展分析法的剩余寿命为2.64×106次。
基金supported by the National Natural Science Foundation of China,Nos.82271447,81771382the National Key Research and Development Program of China,No.2019 YFE0115900the"New 20 Terms of Universities in Jinan,No.202228022 (all to ZZ)。
文摘α-Synuclein is a protein that mainly exists in the presynaptic terminals.Abnormal folding and accumulation of α-synuclein are found in several neurodegenerative diseases,including Parkinson’s disease.Aggregated and highly phospho rylated a-synuclein constitutes the main component of Lewy bodies in the brain,the pathological hallmark of Parkinson s disease.For decades,much attention has been focused on the accumulation of α-synuclein in the brain parenchyma rather than considering Parkinson s disease as a systemic disease.Recent evidence demonstrates that,at least in some patients,the initial α-synuclein pathology originates in the peripheral organs and spreads to the brain.Injection of α-synuclein preformed fibrils into the gastrointestinal tra ct trigge rs the gutto-brain propagation of α-synuclein pathology.However,whether α-synuclein pathology can occur spontaneously in peripheral organs independent of exogenous α-synuclein preformed fibrils or pathological α-synuclein leakage from the central nervous system remains under investigation.In this review,we aimed to summarize the role of peripheral α-synuclein pathology in the pathogenesis of Parkinson’s disease.We also discuss the pathways by which α-synuclein pathology spreads from the body to the brain.