Quantitative studies of the regular distribution pattern for Salmonella enteritidis in the internal organs of mice after oral challenge by a specific real-time polymerase chain reaction

AIM： To identify and understand the regular distribution pattern for Salmonella enteritidis （S. enteritidis） in the internal organs of mice after an oral challenge over a 3 wk period. METHODS： Assays based on the serovar-specific DNA sequence of S. enteritidis from GenBank, and a serovar-specific real-time, fluorescence-based quantitative polymerase chain reaction （FQ-PCR） were developed for the detection of S. enteritidis. We used this assay to detect genomic DNA of S. enteritidis in the blood and the internal organs, including heart, liver, spleen, kidney, pancreas, and gallbladder, from mice after oral challenge at different time points respectively.RESULTS： The results showed that the spleen was positive at 12 h post inoculation （PI）, and the blood was at 14 h PI. The organism was detected in the liver and heart at 16 h PI, the pancreas was positive at 20 h PI, and the final organs to show positive results were the kidney and gallbladder at 22 h PI. The copy number of S. enteritidis DNA in each tissue reached a peak at 24-36 h PI, with the liver and spleen containing high concentrations of S. enteritidis, whereas the blood, heart, kidney, pancreas, and gallbladder had low concentrations. S. enteritidis populations began to decrease and were not detectable at 3 d PI, but were still present up to 12 d PI in the gallbladder, 2 wk for the liver, and 3 wk for the spleen without causing apparent symptoms.CONCLUSION： The results provided significant data for understanding the life cycle of S. enteritidis in the internal organs, and showed that the liver and spleen may be the primary sites for setting itself up as a commensa over a long time after oral challenge. Interestingly, it may be the first time reported that the gallbladder is a site of carriage for S. enteritidis over a 12 d period. This study will help to understand the mechanisms of action of S. enteriCdis infection in vivo.

Multifractal characteristics and spatial variability of soil particle-size distribution in different land use patterns in a small catchment of the Three Gorges Reservoir Region,China

Characterizing soil particle-size distribution is a key measure towards soil property.The purpose of this study was to evaluate the multifractal characteristics of soil particle-size distribution among different land-use from a purple soil catchment and to generalize the spatial variation trend of multifractal parameters across the catchment.A total of 84 soil samples were collected from four kinds of land use patterns(dry land,orchard,paddy,and forest)in an agricultural catchment in the Three Gorges Reservoir Region,China.The multifractal analysis method was applied to quantitatively characterize the soil particle size distribution.Six soil particle size distribution(PSD)multifractal parameters(D(0),D(1),D(2),(35)a(q),(35)f[a(q)],α(0))were computed.Additionally,a geostatistical analysis was employed to reveal the spatial differentiation and map the spatial distribution of these parameters.Evident multifractal characteristics were found.The trend of generalized dimension spectrum of four land use patterns was basically consistent with the range of 0.8 to 2.0.However,orchard showed the largest monotonic decline,while the forest demonstrated the smallest decrease.D(0)of the four land use patterns were ranked as:dry land<orchard<forest<paddy,the order of D(1)was:dry land<paddy<orchard<forest,D(2)presented a rand-size relationship as dry land<forest<paddy<orchard.Furthermore,all land-use patterns presented asΔf[α(q)]<0.The rand-size relationship ofα(0)was same as D(0).The best-fitting model for D(0),D(1),D(2)andΔf[α(q)]was spherical model,forΔα(q)was gaussian model,and forα(0)was exponential model with structure variance ratio was 1.03%,49.83%,0.84%,1.48%,22.20%and 10.60%,respectively.The results showed that soil particles of each land use pattern were distributed unevenly.The multifractal parameters under different land use have significant differences,except forΔα(q).Differences in the composition of soil particles lead to differences in the multifractal properties even though they belong to the same soil texture.Farming behavior may refine particles and enhance the heterogeneity of soil particle distribution.Our results provide an effective reference for quantifying the impact of human activities on soil system in the Three Gorges Reservoir region.

On Spatial Distribution Pattern of Broccoli Clubroot Disease and and the Sampling Technique

[Objective] To further improve the prediction and forecast and continuous control ability of broccoli clubroot disease. [Methods] The spatial distribution pattern of diseased or infected plants was analyzed using the least square method, fre- quency distribution, aggregation index, m＊-m regression analysis and Taylor＇s pow- er law model. [Result] The field distribution of broccoli plants with clubroot disease tended to be aggregated distribution, m＇-m regression analysis showed that the el- ementary composition of the spatial distribution of diseased or infected plants was individual colony, the individuals attracted each other; the disease had obvious dis- ease focus in the field, and the individual colony showed uniform distribution pattern in the field. Taylor＇s power law showed that the spatial pattern of individual dis- eased or infected plant with clubroot disease tended to be uniform distribution with the increase of the density. On the basis of this, Iwao optimal theoretical sampling model and sequential sampling model were established, namely N =273.954 1/m- 59.698 5, To （N）=0.368 4N±1.926 8√N, respectively, it meant that when surveying N plants, if the accumulative incidence rate exceeded upper bound, the field can be set as control object; if the accumulative incidence rate didn＇t reach lower bound, it can be set as uncontrol field; if the accumulative incidence rate was between upper bound and lower bound, it should be surveyed continuously until the maximum sample size （mo=0.368 4） appeared, that was, the disease incidence was 15%, so the sampling number should be 684 plants. [Conclusion] The research results had very important instructive meaning for disease control.

Spatial Distribution Pattern and Application of Fourth-Generation Mature Larvae of Cotton Bollworm(Helicoverpa armigera Hübner) in Corn Field

[ Objective] The paper was to study the spatial distribution pattern of fourth-generation mature larvae of cotton bellworm in corn field. [ Method] The plots with different occurrence densities of fourth-generation cotton bollworm were investigated from August to September in 2009. Six groups of sampling data were obtained, and seven indicators including aggregation index method, Iwao method and Taylor method, etc. were used to determine its spatial distribution pattern. [ Result ] Aggregation index test showed that in all plots, Moore I 〈 0, Lloyed m＊/m 〈 1, Kuno Ca 〈 0, diffusion coefficient C 〈 1, diffusion index 16 〈 1, negative binomial distribution K 〈 0, indicating that mature larvae of cotton bollworm showed uniform distribution in summer corn. Iwae regression equation of fourth-genera- tion mature larvae of cotton boUworm in summer corn was m ＊ = 0. 090 6 ＋ 0. 766 9 m, r = 0. 986 3, indicating that the basic components of cotton bollworm distribu- ted was single individual, and mature larvae of cotton bollworm in summer corn showed uniform distribution. The optimal sampling number of fourth-generation ma- ture larvae of cotton bollworm in corn under different population densities could be calculated using formula N1 = （ 1. 090 6/m -0. 233 1 ）/D2. [ Conclusion] The result provided basis for accurate evaluation of population quantities and variation law of cotton boUworm, as well as prediction and control of the pest.

Preliminary Study on Spatial Distribution Pattern of Endoclyta signifer Walker Larva

[ Objective ] The paper was to study the spatial distribution of Endoclyta signifer Walker Larva. [ Method] The larvae distribution in eucalyptus planta- tion in Beiliu and Luchuan counties of Guangxi province were investigated. Ten standard plots were set, and the spatial distribution pattern of E. signifer larva was confirmed using six different aggregation indexes and regression model analysis method. [ Result] The larvae mainly distributed in the base of tnmk lower than 1 m, and its spatial distribution pattern was mainly aggregated distribution, which was caused by the enviromnental factors. The optimum sampling formula of E. signifer larva under different population densities was n = t2 （ 1.1/m ＋ 0. 674 4）/D2, and limited sequential sampling decision model was T＇0（n）, T＇0（n） = n ＋ 1. 332√n（ m0 = 1 ）. [ Conclusion] The result provided scientific basis for sampling and forecasting of E. signifier.

Spatial Distribution Pattern of Overwintering Larvae of Dendrlimus punctatus

Based on the data of mean population density of overwintering larvae of Dendrolimus punctatus in Shatang forest farm in Guangxi Province, the spatial pattern of overwintering larva of D. punctatus were analyzed by the distribution index and regression model method. The results showed that the spatial pattern of overwintering larvae of D. punctatus assumed the aggregation pattern, the basic component of distribution was individual group. The optimal sampling number of forest survey and the sequential sampling analysis were presented, and the upper and low bound index for controlling D. punctatus were put forward to provide certain theoretical basis for integrated pest management.

Spatial patterns of farmland abandonment and its impact factors in the central Three Gorges Reservoir Area

The severe farmland abandonment phenomena in China have tremendously decreased the farmland usage efficiency and caused a series of profound ecological and social outcomes. The complexity of farmland abandonment necessitates a systematic research on its patterns and impact factors to underpin the dynamics of problem as well as potential solutions. This paper took Wanzhou district in the central part of Three Gorges Reservoir Area as an example to study the spatial distribution patterns of abandoned farmland at three scales, i.e., town, village, and plot, and analyzed the influence from labor transfer and farmland circulation. We conducted a survey on the distribution patterns of abandoned farmland via questionnaires at town and village scales, together with the modeling and interpretation of high-resolution aerial images taken by unmanned aerial vehicles at plot scale. Our research discloses a strong relationship between the distribution of abandoned farmland and the distance from population center at different scales. At eithertown or village scale, the abandonment proportion was recognized the highest in mid-distance areas and lowest in close-distance areas, and the per capita area of abandoned farmland increased with the distance to downtown or township. At plot scale, abandonment proportion presented exponential growth with the distance to village center, whereas the abandoned farmland was mainly distributed in the areas with distance above 500 m from the village center. Moreover, we noticed that labor transfer and farmland circulation are two important factors that influence farmland abandonment. Labor's outbound transfer distance has a positive linkage with the possibility of farmland abandonment. Finally, it is recommended that development and adoption of corresponding policies on local urbanization and farmland circulation will facilitate to alleviate farmland abandonment.

Spatial Distribution Pattern and Sampling Technology of Soritia leptalina Larvae on Quercus aquifolioides

Soritia leptalina endangers mainly Quercus aquifolioides, and it became particularly rampant on Q. aquifolioides in Linzhi area, Tibet. By applying field investigation and lab analysis, this study explored the spatial distribution type and sampling technology of S. leptalina larvae on Q. aquifolioides. The results showed that insect density in the upper layer of tree canopy is higher than that in the middle and lower layer,that in the upper, middle and lower layer of different levels varies greatly, and insect density in different directions follows the sequence: east > north > west > south; spatial distribution of S. leptalina on Q.aquifolioides belongs to aggregation type, larvae show mutual repulsion; aggregation of S. leptalina is the result of biological characteristics of its larvae and the habitat environment; taking Iwa regression model as the basis, the optimal sampling quantity is determined. The study disclosed the spatial distribution rules of S.leptalina on Q. aquifolioides, and on the basis of which could provide the theoretical basis for preventing Q.aquifolioides from the damage of S. leptalina, and also scientific support for studying the spatial distribution pattern of this insect and its natural enemies on other hosts.

Spatial Distribution Pattern and Han Subgroup Characteristics of Traditional Villages in Guangdong

A total of 126 traditional villages in Guangdong were selected for research to analyze their spatial distribution pattern, spatial autocorrelation, and Han subgroup characteristics through the spatial analysis with GIS. The results showed that the traditional villages in Guangdong were distributed compactly, which was similar to that in other provinces; on the prefecture-level city scale, they were mainly distributed in Meizhou, Qingyuan, Zhanjiang, and Zhaoqing, showing a central distribution; on the regional scale, they were mainly distributed in northern Guangdong and the Pearl River Delta, indicating an unbalanced distribution; their density of spatial distribution showed a significant difference with a provincial distribution density of 0.59 per thousand km^2 and the highest distribution density of 2.14 per thousand km^2; there were four gathering areas accommodating many traditional villages, namely Meizhou-Chaoshan area, Guangfo-Dongguan area, Leizhou in Zhanjiang, and Lianzhou in Qingyuan; the spatial distribution of traditional villages, which presented a pattern of hot spot—sub-hot spot—sub-cold spot—cold spot, had a significant global spatial autocorrelation, and areas in similar sizes were tending towards gathering; traditional villages in Guangdong belonged to the Cantonese-speaking group and Hakka-speaking group with a density of 0.59 per thousand km^2 and 0.48 per thousand km^2 respectively, and Han subgroup with the highest distribution density was the minority areas of 1.31 per thousand km^2, followed by Shaozhou dialect area with a density of 1.00 per thousand km^2.

Spatial Distribution Patterns of Cultural Facilities in Shenzhen Based on GIS and Big Data

As the city’s soft powef,culture is the basic core of urban competitiveness. The spatial concentration of cultufal facilities can show the development status of the dtfs cultural functions in the spatial kyout. In this paper,POI data of Baidu Maps were used to conduct kernel density analysis and comprehensive evaluation of the spatial distribution of cultural facilities in Shenzhen; mean center point method was used to measure the degree of deviation between Shenzhen population centers and cultural facilities centers; the nearest distance method was used to measure the spatial distribution of cultural facilities space and the accessibility to geometdc centers of streets. The results showed that among the four types of cultural facilities in Shenzhen, librades were the most concentrated, accounting for 63% of the total number of cultural facilities, followed by cultxite and arts centers, museiims, and galleries; tiiere was a great disparity in the number of cultural facilities in various districts and subdistricts in Shenzhen, with a clear agglomeration effect in space; from the district level, cultural faculties spatially distributed from the southwest to the northeast by three gradients; from the street leviel, cultural facilities showed the distribution pattern of ^decreasing from west to eastf and a high d^tee of recognition for convenient transportation; the cultural facilities inside the Shenzhen Special Economic Zone （except Yantdan District） were more reasonable in spatial distribution, and the number of people served was more than that of cultural Realities outside the Shenzhen Special Economic Zone, while residents outside the Shenzhen Special Economic Zone enjoyed significantly more cultutal facilities than that inside the Shenzhen Special Economic Zone; the goal of the e￡10-min cultutal dtdeJ, in Shenzhen had been initially completed, that is, residents could reach 72.41% of the streets with cultural facilities within a 10-min ／raJk, but thefe were still 50% districts and 5.1% streets that wefe equipped with, unreasonably distributed cultufal facilities or few cultufal facilities.

Spatial pattern analysis and associations of different growth stages of populations of Abies georgei var.smithii in Southeast Tibet,China

Abies georgei var.smithii is an important plant species in Southeast Tibet,China.It has high ecological value in terms of biodiversity protection,as well as soil and water conservation.We analyzed the spatial pattern and associations of A.georgei var.smithii populations at different growth stages by using Ripley's L function for point pattern analysis.The diameter structure was a nearly reverse 'J' shape.The amount of saplings and medium-sized trees accounts for a large part of the entire population,suggesting a high regeneration rate and an expanding population.In the transition from saplings to medium trees or to large trees,saplings show a significant aggregation distribution at small scales,while medium trees and large trees show a random distribution.There are significant inverse associations between saplings and medium trees and large trees at small scales,while there are no obvious associations between medium trees and large trees.The natural regeneration was affected by interspecific competition,and it was also affected by intraspecific competition.The joint effects of biological characteristics and environmental factors contribute to the spatial distribution pattern and associations of this A.georgei var.sm ithii population.

Space-time Dynamics of Dendroctonus valens Population in China and Spatial Sampling Technique based on Its Spatial Distribution Pattern

Red turpentine beetle （RTB）, Dendroctongs valens LeConte, is a destructive forest invasive species in China, it mainly attacks Pings tabuliformis and P. bungeana. So far it has spread rapidly to the provinces of Shanxi, Hebei, Henan, Shanxi and Beijing since its first outbreak in Shanxi Province in 1998, and has caused extensive tree mortality. Space-time dynamics of D. valens population and spatial sampling technique based on its spatial distribution pattern were ana- lyzed using geostatistical methods in the pure P. tabuliforis forests and mixedwood stands which were at different damage levels. According to the spatial distribu- tion of D. valeas population, the specific spatial sampling technique was also studied, and then was compared with traditional sampling technique. The spatial sam- piing technique combined with sampling theory and the biological characteristics of D. valens population, which not only could calcnlate the error of the sampling, but also could discuss the optimal sampling number and the optimum size of plot according to different damage levels and different stand types. This helps to explain population expansion and colonization mechanism of D. valens, and to provide a good reference for adopting snitable control measures.

Spatial Distribution of Mature Larvae of Obolodiplosis robiniae and Damage Regularity of Its First Generation Larvae on Leaves of Locust( Robinia pseudoacacia)

[ Objective] The paper was to explore the population dynamics of Obolodiplosis robiniae （Haldemarm）. [ Method ] The spatial distribution of mature larvae of O. robiniae and the damage regularity of its first generation larvae on leaves of locust were studied in Fangshan District of Beijing City. [ Result] The spa- tial distribution of mature larvae of O. robin/ae in the forest was sigrrificanfly higher than those in forest edge and periphery of forest edge. The attack of the first generation larvae was mainly concentrated in the lower part of tree crown. [ Conclusion] The study provides theoretical basis for the effective control against O. robiniae.

Spatial Pressure Distribution of Cultivated Land and the Analysis of Food Safety in Kunming City,China

The social and economical condition,cultivated land resources and general situation of grain production of research area are introduced.According to relevant data of cultivated land of Kunming City in 2006,taking the minimum per capita cultivated land and index model of cultivated land pressure,and combining with two conditions of per capita grain demand which are 300 kg and 400 kg,the comparative research method was used to calculate and analyze the minimum per capita cultivated land and index model of cultivated land pressure.The results show that when per capita grain demand-Gr is 300 kg,the per capita cultivated land in Kunming City has regional differences,reflects different regular patterns of spatial distribution,and presents obvious and typical radial distribution.Urban area of Kunming has the lowest point and be taken as the center,per capita cultivated land decreases progressively from suburban to the periphery;when per capita grain demand-Gr is 400 kg,under the same consumption level,cultivated land pressures of each county in Kunming City are different,and so are the driven factors;when per capita grain demand-Gr is 300 kg or 400 kg,the indexes of cultivated land pressure in Kunming are all greater than 1,cultivated land pressure is enormous,the indexes of cultivated land pressure of each county(district) have two conditions-greater than 1 and less than 2,and the reasons differ from one another.Combining with the spatial distribution characteristics of the cultivated land and food and the situation of industrial development,the countermeasures and suggestions are put forwarded to make full use of back-up cultivated land,to plan the distribution of industrial structure in each county(district) of Kunming City as a whole,to dispose cultivated land resources reasonably and so on.

Space-time Distribution Pattern of Monochamus alternatus in Wanding City

[Objective] The purpose was to study space-time distribution pattern of Monochamus alternatus in Wanding City. [Method] Based on data about insect situation supervision on M. alternatus in Wanding City, using related mathematical statistics methods including linear regression analysis and spatial distribution pattern of insect population, the relationship between emergence period, emergence quantity and temperature of M. alternatus adult, spatial distribution pattern of adults and their life history were studied. [Result] M. alternatus occurred one generation per year in Wanding city, June was the maximum period of adults, which was also the key trapping period. Spatial distribution of M. alternatus was accumulative. [Conclusion] This study provided theoretical basis for further control on M. alternatus for forestry bureau of Wanding City.

Population structure and distribution pattern of Taxus cuspidata in Muling region of Heilongjiang Province, China

An investigation was conducted on distribution pattern, site condition and population structure of yew Taxus cuspidata Sieb. et Zucc. in Muling Forest Bureau of Heilongjiang Province, China in April, 2005. Results showed that yew is mainly distributed under the main storey of natural mixed forest of conifer and broadleaf, the soil moisture content of the yew site is high （40%-60%）, the pH value of soil is relatively lower （4.7-5.5）, and that the population structure of wild yew is not rational, belonging to the degeneration population, which is one of the reasons leading to the population decline. Although the site conditions of Muling area are suitable for the growth of wild yew, the population of wild yew shows a decline tendency, due to the fact that the middle-sized adult yew trees have been cut, young yews are often grazed by wildlife, and that the trunks of adult yew tend to be hollow.

Single-trial EEG-based emotion recognition using temporally regularized common spatial pattern

This study addresses the problem of classifying emotional words based on recorded electroencephalogram （EEG） signals by the single-trial EEG classification technique. Emotional two-character Chinese words are used as experimental materials. Positive words versus neutral words and negative words versus neutral words are classified, respectively, using the induced EEG signals. The method of temporally regularized common spatial patterns （TRCSP） is chosen to extract features from the EEG trials, and then single-trial EEG classification is achieved by linear discriminant analysis. Classification accuracies are between 55% and 65%. The statistical significance of the classification accuracies is confirmed by permutation tests, which shows the successful identification of emotional words and neutral ones, and also the ability to identify emotional words. In addition, 10 out of 15 subjects obtain significant classification accuracy for negative words versus neutral words while only 4 are significant for positive words versus neutral words, which demonstrate that negative emotions are more easily identified.

Spatial distribution features of sequence types of moderate and strong earthquake in Chinese mainland

Based on 294 earthquake sequences with magnitude greater than or equal to 5.0 occurred in Chinese mainland since 1970, the spatial distribution features of sequence types have been studied. In southwestern China, it takes mainshock-aftershock sequence type （MAT） as the major in Chuan-Dian rhombic block and concerned Xianshuihe-Anninghe-Xiaojiang seismic belt, as well as in Jinshajiang-Honghe seismic belt. Multiple mainshock type （MMT） mainly distributes in western Yunnan, and Longlin and Lancang areas in Tengchong-Baoshan block in west of Nujiang-Lancangjiang fault zone. A few isolated earthquake type （IET） mainly occurred in northwestern Sichuan and there is no IET occurred in Yunnan region. In northwestern China, it takes mainshock-aftershock sequence type （MAT） as the major in west segment of South Tianshan in Xinjiang region. Some MMT also occurred in this area in the intersection of Kalpin block and the Puchang fault zone. It takes IET as the major in middle Tianshan in Xinjiang. Along the Qilianshan seismic belt, most of sequences are MAT. In Qinghai region, it takes MAT as the major, but the regional feature of the spatial distribution of sequence types is not very clear. In North China, it takes MAT as the major in Yinshan-Yanshan-Bohai seismic belt, north edge of North China, and in Hebei plain seismic belt, as well as in sub-plate of lower river area of Yangtze River. In intersection of north segment of Shanxi seismic belt and the NW-trending Yinshan-Yanshan-Bohai seismic belt, there are several moderate or strong MMT with magnitude from 5.0 to 6.0 occurred. In south of North China around the latitude line of 35°N, it takes IET as the major. The spatial distribution of sequence types is relevant to the patterns of tectonic movements. MAT is mostly produced by the ruptures of locked units or asperities or the neonatal separating segments inside the fault zones. MMT is generally relevant to the conjugate structures or intersection of many tectonic settings. Further extension of simple fault often produces IET. Spatial distribution of sequence types is also correlative to the regional and deep environment of crustal medium to some extent. MAT mainly distributes in high velocity area in upper crust or in the transition zone between high velocity area and low velocity area, MMT mostly occurred in the low velocity area in upper crust.

Population structure and spatial pattern analysis of Quercus aquifolioides on Sejila Mountain,Tibet,China

Understanding population structure provides basic ecological data related to species and ecosystems.Our objective was to understand the mechanisms involved in the maintenance of Quercus aquifolioides populations.Using a 1 ha permanent sample plot data for Q.aquifolioides on Sejila Mountain,Tibet Autonomous Region(Tibet),China,we analyzed the population structure of Q.aquifolioides by combining data for diameter class,static life table and survival curve.Simultaneously,the spatial distribution of Q.aquifolioides was studied using Ripley’s L Function in point pattern analysis.The results showed:(1) Individuals in Q.aquifolioides populations were mainly aggregated in the youngest age classes,that accounted for94.3% of the individuals; the older age classes had much smaller populations.Although the youngest age classes(ClassesⅠ and Ⅱ) had fewer individuals than Class Ⅲ,the total number of individuals in classes Ⅰ and Ⅱ was also greater than in classes Ⅳ to Ⅸ.In terms of tree height,fewsaplings,more medium-sized saplings and few large-sized trees were found.The diameter class structure of Q.aquifolioides populations formed an atypical ‘pyramid’type; the population was expanding,but growth was limited,tending toward a stable population.(2) Mortality of Q.aquifolioides increased continuously with age; life expectancy decreased over time,and the survivorship curve was close to a Deevey I curve.(3) The spatial distribution pattern of Q.aquifolioides varied widely across different developmental stages.Saplings and medium-sized tree showed aggregated distributions at the scales of 0–33 m and 0–29 m,respectively.The aggregation intensities of saplings and medium-sized trees at small scales were significantly stronger than that of large-sized trees.However,large-sized trees showed a random distribution at most scales.(4) No correlation was observed among saplings,medium-and large-sized trees at small scales,while a significant and negative association was observed as the scale increased.Strong competition was found among saplings,medium-and large-sized trees,while no significant association was observed between medium-and largesized trees at all scales.Biotic interactions and local ecological characteristics influenced the spatial distribution pattern of Q.aquifolioides populations most strongly.

Avalanching patterns of irregular sand particles in continual discrete flow

We investigate the flow patterns of irregular sand particles under avalanching mode in a rotating drum by using the spatial filtering velocimetry technique.By exploring the variations of velocity distribution of granular flow,we find a type of avalanching pattern of irregular sand particles which is similar to that of spherical particles flow.Due to the fact that the initial position of avalanche in this pattern locates at the middle of the drum and the avalanche propagates toward the edge area gradually,we named it as mid-to-edge avalanching pattern.Furthermore,we find another avalanching pattern which slumps from the edge and propagates toward the opposite edge of the flow surface,named as edge-to-edge pattern.By analyzing the temporal and spatial characteristics of these two types of avalanching patterns,we discover that these two types of avalanche patterns are caused by that the avalanching particles constantly perturb the axial adjacent particles.Thus,the particles on the flow surface are involved in avalanching sequentially in order of the axial distance from the initial position.