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The use of feed-grade amino acids in lactating sow diets
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作者 Laura Greiner Pairat Srichana +5 位作者 James L.Usry Casey Neill Gary L.Allee Joseph Connor Kevin J.Touchette Christopher D.Knight 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2018年第2期399-406,共8页
Background: The use of feed grade amino acids can reduce the cost of lactation feed. With changing genetics,increasing feed costs, and higher number of pigs weaned with heavier wean weights further evaluation of highe... Background: The use of feed grade amino acids can reduce the cost of lactation feed. With changing genetics,increasing feed costs, and higher number of pigs weaned with heavier wean weights further evaluation of higher inclusion levels of feed-grade amino acid in lactation diets than previously published is warranted. Two experiments(Exp.) were conducted to determine the optimal inclusion level of L-lysine HCl to be included in swine lactation diets while digestible lysine levels remain constant across dietary treatments and allowing feed grade amino acids to be added to the diet to maintain dietary ratios relative to lysine to maximize litter growth rate and sow reproductive performance. Furthermore, the studies were to evaluate minimal amino acid ratios relative to lysine that allows for optimal litter growth rate and sow reproductive performance.Results: Exp. 1: Increasing L-lysine HCl resulted in similar gilt feed intake, litter, and reproductive performance.Average litter gain from birth to weaning was 2.51, 2.49, 2.59, 2.43, and 2.65 kg/d when gilts were fed 0.00, 0.075,0.150, 0.225, and 0.30% L-lysine HCl, respectively. Exp. 2: The average litter gain from birth to weaning was 2.68,2.73, 2.67, 2.70, and 2.64 kg/d(P < 0.70) when sows were fed 0.1, 0.2, 0.3, 0.4, and 0.4% L-lysine HCl plus valine,respectively. No other differences among dietary treatments were observed.Conclusions: Collectively, these studies demonstrate corn-soybean meal based lactation diets formulated with a constant SID lysine content for all parities containing up to 0.40% L-lysine HCl with only supplemental feed grade threonine and a methionine source have no detrimental effect on litter growth rate and subsequent total born. 展开更多
关键词 amino acid ratio LACTATION L-LYSINE SOW
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Branched-chain amino acids to tyrosine ratio value as a potential prognostic factor for hepatocellular carcinoma 被引量:10
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作者 Toru Ishikawa 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第17期2005-2008,共4页
The prognosis of hepatocellular carcinoma(HCC) depends on tumor extension as well as hepatic function.Hepatic functional reserve is recognized as a factor affecting survival in the treatment of HCC;the ChildPugh class... The prognosis of hepatocellular carcinoma(HCC) depends on tumor extension as well as hepatic function.Hepatic functional reserve is recognized as a factor affecting survival in the treatment of HCC;the ChildPugh classif ication system is the most extensively used method for assessing hepatic functional reserve in patients with chronic liver disease,using serum albumin level to achieve accurate assessment of the status of protein metabolism.However,insuff icient attention has been given to the status of amino acid(AA) metabolism in chronic liver disease and HCC.Fischer's ratio is the molar ratio of branched-chain AAs(BCAAs:leucine,valine,isoleucine) to aromatic AAs(phenylalanine,tyrosine) and is important for assessing liver metabolism,hepatic functional reserve and the severity of liver dysfunction.Although this ratio is diff icult to determine in clinical situations,BCAAs/tyrosine molar concentration ratio(BTR) has been proposed as a simpler substitute.BTR correlates with various liver function examinations,including markers of hepatic f ibrosis,hepatic blood flow and hepatocyte function,and can thus be considered as reflecting the degree of hepatic impairment.This manuscript examines the literature to clarify whether BTR can serve as a prognostic factor for treatment of HCC. 展开更多
关键词 Branched-chain amino acids to tyrosine ratio Fischer's ratio Prognostic factor Hepatocellular carcinoma
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Effects of Amino Acid Foliar Fertilizer on the Yield and Quality of Different Vegetables 被引量:2
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作者 SUN Mei SUN Geng +2 位作者 MA Hao-liu LUO Zun-chang LIU Jie 《Agricultural Science & Technology》 CAS 2018年第6期10-15,共6页
In order to explore the effects of amino acid foliar fertilizer on the yield and quality of different vegetables, we tested the spraying of an amino acid foliar fertilizer(Wanfeng 2) onto the leaves of cucumbers, eggp... In order to explore the effects of amino acid foliar fertilizer on the yield and quality of different vegetables, we tested the spraying of an amino acid foliar fertilizer(Wanfeng 2) onto the leaves of cucumbers, eggplants and water spinach through contrast experiment in a greenhouse. The results showed that the yield increments of amino acid foliar fertilizer treatments in cucumbers, eggplants and water spinach were 11.57%, 4.43% and 12.41% respectively, and the output-input ratios were 64.52 ∶ 1, 53.31 ∶ 1 and 55.14 ∶ 1 respectively. Amino acid foliar fertilizer can influence the quality of greenhouse vegetables. The total sugar content of the cucumbers, eggplants, and water spinach significantly increased by 12.00%, 22.30%and 12.10% respectively. The vitamin C content increased by 4.76%, 24.80%, and10.90% respectively compared with the control. In conclusion, the spraying of amino acid foliar fertilizer could improve the yield and the nutritive quality of vegetables such as cucumbers, eggplants and water spinach. 展开更多
关键词 Foliar fertilizer amino acid Vegetable quality YIELD Output-input ratios
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Early administration of branched-chain amino acid granules 被引量:3
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作者 Toru Ishikawa 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第33期4486-4490,共5页
The effect of malnutrition on survival in patients with decompensated liver cirrhosis has not been well defined. Nutritional intervention with branched-chain amino acid (BCAA) can increase serum albumin concentration ... The effect of malnutrition on survival in patients with decompensated liver cirrhosis has not been well defined. Nutritional intervention with branched-chain amino acid (BCAA) can increase serum albumin concentration in patients with decompensated cirrhosis but its effects on survival are unclear. The BCAA to tyrosine ratio (BTR) is a surrogate marker (the normal range of BTR is between 4.41 and 10.05, and a Fischer's ratio of 1.8 corresponds to a BTR of 3.5) in patients with decompensated liver cirrhosis, and BCAA inhibits hepatic carcinogenesis in patients with compensated cirrhosis. This review discusses data regarding the effect of early administration of BCAA granules based on the ratio of BCAA to BTR on prognosis in patients with cirrhosis. 展开更多
关键词 Branched-chain amino acid to tyrosine ratio Branched-chain amino acid granules Liver cirrhosis Nutritional intervention Malnutrition Quality of life Albumin Cancer onset
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Ratio of hydantoinase and N-carbamoylase:key to preparation of D-amino acid from 5-monosubstituted hydantoin 被引量:1
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作者 姚忠 竺凯 +2 位作者 周华 韦萍 欧阳平凯 《化工学报》 EI CAS CSCD 北大核心 2005年第1期148-151,共4页
关键词 取代 海因酶 制备 酸水解 氨基酸 比例
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An Elevated Dietary Cysteine to Methionine Ratio Does Not Impact on Dietary Methionine Efficiency and the Derived Optimal Methionine to Lysine Ratio in Diets for Meat Type Chicken 被引量:1
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作者 Daulat Rehman Khan Christian Wecke Frank Liebert 《Open Journal of Animal Sciences》 2015年第4期457-466,共10页
Optimal dietary methionine (Met) to lysine (Lys) ratio in presence of elevated dietary cysteine (Cys) levels was derived for meat type growing chicken. Twelve averaged weighed Ross 308 birds (each 50% of male and fema... Optimal dietary methionine (Met) to lysine (Lys) ratio in presence of elevated dietary cysteine (Cys) levels was derived for meat type growing chicken. Twelve averaged weighed Ross 308 birds (each 50% of male and female per dietary treatment) were utilized in N balance trials. During starter (d10 - 20) and grower period (d25 - 35) five dietary treatments were used. Diets based on uniform mixtures of maize, wheat, soybean meal, potato protein and fish meal were supplemented with crystalline amino acids (AA). In diets 1 - 3, the dietary Cys to Met ratio was set as 85, 95 and 105 to 100, respectively. Diet 4, at a Cys to Met ratio of 105 to 100, was additionally supplemented with betaine (BET) as methyl group donor. Diets 1 - 4 were limiting in Met, diet 5 without L-Lys·HCl addition was limiting in Lys. Individual N-balance data per treatment were utilized for assessing protein quality and efficiency of dietary Met (Diets 1 - 4) or Lys (Diet 5) based on “Goettingen approach”. Elevated dietary Cys supply and supplemented BET failed to improve both dietary protein quality and Met efficiency. The established optimal Met to Lys ratio was on average 34 to 100 for growing chicken during starter and grower period, respectively. 展开更多
关键词 Growing CHICKEN N Utilization Model amino Acid Efficiency METHIONINE to LYSINE ratio CYSTEINE Betaine
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Using the RNA synthetic activity of glutamate dehydrogenase to illuminate the natural role of the enzyme 被引量:1
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作者 Godson O. Osuji Wenceslaus C. Madu 《Advances in Biological Chemistry》 2012年第4期379-389,共11页
Glutamate Dehydrogenase (GDH;EC 1.4.1.2) catalyzes the reversible amination of α-ketoglutarate to glutamate, and the polymerization of nucleoside triphosphate(s) to RNA. But the natural role of the reversible aminati... Glutamate Dehydrogenase (GDH;EC 1.4.1.2) catalyzes the reversible amination of α-ketoglutarate to glutamate, and the polymerization of nucleoside triphosphate(s) to RNA. But the natural role of the reversible amination reaction is the subject of an expanding conversation. The aim was to illuminate the natural role of GDH through its RNA synthetic activity. Stoichiometric combinations of mineral salts that targeted the GDH subunit compositions were applied to field-cultivated peanuts. GDH of seeds were made to synthesize RNA in the deamination and then in the amination direction. Free amino acids were analyzed by HPLC. Glutamate synthase (GOGAT) was assayed by photometry. Free amino acid yields in-creased from the control’s lowest (9.8 kg·ha–1) and amination-deamination ratio (0.05) through 12.0 - 23.0 kg·ha–1 in the K-, N+K+P+S-, Pi-, N+S-, S-treated peanuts with amination-deamination ratios between 0.6 and 10.0 until at the P+K-treated peanut which had the highest amino acid yield (52.4 kg·ha–1) and the highest amination-deamination ratio (61). The Km and Vmax values of GOGAT were within the normal range. Yields of free amino acids resulting from GDH aminating activity increased from <1.0 kg·ha–1 in the control, through 2.2 in the N+S-, 6.84 in the P+N-, 17.3 in the N-, to 42.6 kg·ha–1 in the P+K- treated peanut. These results show that the natural role of the GDH amination activity is to assimilate escalating multiples of the quantities of NH4+ ion as assimilated via the GS-GOGAT pathway. Peanut protein yields increased in parallel with GDH aminating activities and free amino acid yields such that the control peanut had the lowest protein (<26.0 kg·ha–1) and the yields increased exponentially (500 - 600 kg·ha–1) through the K-, P+S-, Pi-, N-treated to 910 kg·ha–1 in the P+K-treated peanut with the highest aminating activity of GDH. The ability of GDH aminating activity to escalate protein yields of food crops could be employed to address proteinenergy malnutrition syndrome of developing nations. 展开更多
关键词 PEANUT STOICHIOMETRIC Mineral Salt Combinations GDH Amination-Deamination ratio Glutamate SYNTHASE amino Acid and Protein Yields ha–1
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Reversed-phase fused-core HPLC modeling of peptides 被引量:3
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作者 Matthias D'Hondt Bert Gevaert +5 位作者 Sofie Stalmans Sylvia Van Dorpe Evelien Wynendaele Kathelijne Peremans Christian Burvenich Bart De Spiegeleer 《Journal of Pharmaceutical Analysis》 SCIE CAS 2013年第2期93-101,共9页
Different fused-core stationary phase chemistries(C18,Amide,Phenyl-hexyl and Peptide ES-C18) were used for the analysis of 21 structurally representative model peptides.In addition,the effects of the mobile phase co... Different fused-core stationary phase chemistries(C18,Amide,Phenyl-hexyl and Peptide ES-C18) were used for the analysis of 21 structurally representative model peptides.In addition,the effects of the mobile phase composition(ACN or MeOH as organic modifier;formic acid or acetic acid,as acidifying component) on the column selectivity,peak shape and overall chromatographic performance were evaluated.The RP-amide column,combined with a formic acid-acetonitrile based gradient system,performed as best.A peptide reversed-phase retention model is proposed,consisting of 5 variables:log SumAA,log Sv,clog P,log nHDon and log nHAcc.Quantitative structure-retention relationship(QSRR) models were constructed for 16 different chromatographic systems.The accuracy of this peptide retention model was demonstrated by the comparison between predicted and experimentally obtained retention times,explaining on average 86% of the variability.Moreover,using an external set of 5 validation peptides,the predictive power of the model was also demonstrated.This peptide retention model includes the novel in-silico calculated amino acid descriptor,AA,which was calculated from log P,3D-MoRSE,RDF and WHIM descriptors. 展开更多
关键词 Peptides Fused-core(core-shell core-enhanced poro-shell HALO s) stationary phases RP-HPLC peptide retention model In-silico amino acid descriptor
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不同类型茄子果实主要营养成分和氨基酸含量分析 被引量:1
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作者 惠志明 张树根 +4 位作者 邓晓梅 邢永萍 张秦 王振泉 张军民 《中国蔬菜》 北大核心 2024年第3期97-103,共7页
为了解茄子果实营养品质特性,对3种类型、15个茄子DH系的果实糖度、有机酸含量、可溶性糖含量、糖酸比、可溶性固形物含量、固酸比、游离氨基酸含量进行测定分析。结果表明,3种不同类型茄子的糖度和固酸比由高到低均为紫萼紫黑圆茄>... 为了解茄子果实营养品质特性,对3种类型、15个茄子DH系的果实糖度、有机酸含量、可溶性糖含量、糖酸比、可溶性固形物含量、固酸比、游离氨基酸含量进行测定分析。结果表明,3种不同类型茄子的糖度和固酸比由高到低均为紫萼紫黑圆茄>紫萼紫黑长茄>绿萼紫黑长茄;有机酸含量为绿萼紫黑长茄>紫萼紫黑长茄>紫萼紫黑圆茄;葡萄糖、蔗糖含量均以绿萼紫黑长茄最高,果糖含量以紫萼紫黑圆茄最高;可溶性固形物含量以紫萼紫黑长茄最高;味感为鲜味、甜味的天冬氨酸、丝氨酸、甘氨酸含量由高到低均为紫萼紫黑圆茄>紫萼紫黑长茄>绿萼紫黑长茄,味感为酸甜味、苦味的天冬酰胺、精氨酸含量由高到低均为紫萼紫黑长茄>绿萼紫黑长茄>紫萼紫黑圆茄;紫萼紫黑长茄和绿萼紫黑长茄主要营养成分和游离氨基酸含量的变异度普遍高于紫萼紫黑圆茄。 展开更多
关键词 茄子 糖度 糖酸比 固酸比 游离氨基酸
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新疆不同地区加工专用花生营养成分比较
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作者 罗舒舒 张雨 +3 位作者 张甜甜 代蕾 高建宇 王强 《现代食品科技》 CAS 北大核心 2024年第2期19-28,共10页
花生生长条件和环境不同导致其品质出现差异,分析不同来源花生原料的品质有利于优化原料的选择,为花生产品加工利用提供参考。新疆南、北疆和东疆均有种植花生,但适合新疆不同地区种植的加工专用品种较少,采集新疆不同地区和内地3个省份... 花生生长条件和环境不同导致其品质出现差异,分析不同来源花生原料的品质有利于优化原料的选择,为花生产品加工利用提供参考。新疆南、北疆和东疆均有种植花生,但适合新疆不同地区种植的加工专用品种较少,采集新疆不同地区和内地3个省份的32个样品,对含油量、蛋白质、蔗糖、油酸/亚油酸(O/L)、蛋白亚基、氨基酸等6个指标进行测定、分析比较。结果表明,同一花生品种在不同地区种植后的营养成分含量存在一定差异,整体而言,新疆花生的含油量、蛋白质、蔗糖等指标在不同程度上优于内地,进一步分析得出花生在吐鲁番的含油量、O/L、伴花生球蛋白、总氨基酸含量最高,最高值为55.71%、35.11、37.39%、28.23 g/100 g,在阿拉尔的蛋白质、花生球蛋白含量最高,最高值为22.67 g/100 g、63.28%,在和田的蔗糖、23.5 ku亚基含量最高,为5.07 g/100 g、20.48%,在三坪的37.5 ku亚基含量最高(9.31%)。新疆不同地区不同品种花生的营养成分研究为新疆花生产业区域布局提供参考,为企业选择优质原料、建立原料基地提供依据。 展开更多
关键词 花生 营养成分 含油量 蛋白质 蔗糖 油酸/亚油酸(O/L) 蛋白亚基 氨基酸
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不同豆类蛋白粉的属性和营养消化特性研究
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作者 张莉 季国志 母智深 《粮食与饲料工业》 CAS 2024年第6期19-23,共5页
为了能够在实验研究和产品开发前期对豆类蛋白质原料进行高效的筛选,通过分析5种豆类蛋白粉的氨基酸分布、营养价值、流动性、溶解度、色差、蛋白质消化率和抗氧化能力,可以较为全面地了解每种蛋白粉的应用和营养属性。结果显示,豌豆蛋... 为了能够在实验研究和产品开发前期对豆类蛋白质原料进行高效的筛选,通过分析5种豆类蛋白粉的氨基酸分布、营养价值、流动性、溶解度、色差、蛋白质消化率和抗氧化能力,可以较为全面地了解每种蛋白粉的应用和营养属性。结果显示,豌豆蛋白粉中赖氨酸含量最高,亮度最强;鹰嘴豆蛋白粉中各氨基酸的占比达到了成人氨基酸评分模式的要求,比较氨基酸比值系数分,其营养质量最高;大豆蛋白粉也达到了成人氨基酸评分模式的要求,溶解度和消化率最高,抗氧化能力也最强;蚕豆蛋白粉的流动性最好;绿豆蛋白粉的溶解度和消化率均较低。 展开更多
关键词 豆类蛋白质 氨基酸比值 溶解度 色差 体外消化 抗氧化
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鱼蛋白水溶氨基酸肥的制备及其对植物生长发育的影响
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作者 林湘雪 何咏梅 +3 位作者 辛恺达 张婷 李欣 田宝玉 《福建农业科技》 CAS 2024年第10期21-28,共8页
水溶性氨基酸肥料是一种新型的植物功能性有机肥料。为探究水溶氨基酸肥对植物生长的影响,本文以鱼产品下脚料为原料,通过合理优化料水比、蛋白酶种类和酶量以及水解条件制备水溶性氨基酸肥料。进而通过水培试验以不同稀释倍数的鱼蛋白... 水溶性氨基酸肥料是一种新型的植物功能性有机肥料。为探究水溶氨基酸肥对植物生长的影响,本文以鱼产品下脚料为原料,通过合理优化料水比、蛋白酶种类和酶量以及水解条件制备水溶性氨基酸肥料。进而通过水培试验以不同稀释倍数的鱼蛋白水溶肥对小麦百农矮抗58进行施肥,确定最适稀释倍数,并分别采取喷施与浇施两种方法对番茄新中蔬四号进行施肥,研究水溶性氨基酸肥不同施肥处理对农作物生长发育的影响。结果表明:以1∶3料液比、添加0.1%碱性蛋白酶、50℃酶解4 h条件下,利用鱼蛋白下脚料可以制备可溶氨基酸总量为16.925 mg·g^(-1),且植物易利用氨基酸比例高(约50%)的鱼蛋白水溶肥。水培试验表明鱼蛋白氨基酸水溶肥的最适施用浓度为稀释100倍(DB100)。DB100显著提高了小麦种子萌发率和种子活力指数,促进了小麦幼苗的根长和根冠比(P<0.05)。以DB100作为稀释标准,盆栽番茄喷施氨基酸肥显著提高了番茄幼苗的株高(22.9%)、叶面积(38%)和叶片叶绿素含量(33.3%)(P<0.05),且降低了番茄叶片丙二醛含量(26.7%,P<0.05)。而水溶肥浇施与对照CK相比无显著差异(P>0.05)。盆栽试验说明叶面喷施水溶肥能够直接为植物提供营养物质,植物易吸收和利用,从而促进番茄的生长发育,增强植物叶光合作用效率,提高植物抗氧化能力。综上所述,合理使用水溶氨基酸肥可以显著促进植物种子萌发和幼苗生长,而叶面喷施水溶氨基酸肥显著促进了植物叶片的光合效率和植株的生长。 展开更多
关键词 鱼产品加工下脚料 水溶氨基酸肥制备 稀释倍数 施肥方式 促进植物生长
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Analysis of amino acid enantiomers in ambient aerosols:Effects and removal of coexistent aerosol matrix
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作者 Ying Li Xiaoying Li +5 位作者 Libin Wu Luhan Shi ShanWang Pingqing Fu Yingyi Zhang Senchao Lai 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2024年第3期732-740,共9页
Amino acids(AAs)including D-and L-enantiomers are a group of organic nitrogen species in ambient aerosol.Due to the low abundances of AAs(level of ng/m^(3))and the matrix effects by coexistent components,it is challen... Amino acids(AAs)including D-and L-enantiomers are a group of organic nitrogen species in ambient aerosol.Due to the low abundances of AAs(level of ng/m^(3))and the matrix effects by coexistent components,it is challenging to quantify AA enantiomers in ambient aerosols especially under pollution conditions.In this study,we present an optimized method for analyzing AA enantiomers in atmospheric aerosol samples including a pretreatment process and the detection by high performance liquid chromatography coupled to a fuorescence detector(HPLC-FLD).Matrix effects caused by coexistent chemicals on AA enantiomers analysis in ambient aerosol samples were investigated especially for those collected in pollution episodes.The results revealed that the determination of AA enantiomers is significantly affected by the coexistent organic carbon(as a proxy of organic matter)and water-soluble ion of NH_(4)^(+).To remove the matrix effects,we applied a pretreatment using the solid phase extraction column coupled with alkaline adjustment to sample extract.After pretreatment,18 AAs including 6 pairs of D-and L-enantiomers(i.e.,leucine,isoleucine,valine,alanine,serine,and aspartic acid)can be successfully separated and quantified in aerosol samples by HPLC-FLD.The recoveries are in the range of 67%-106%.This method was successfully applied to the urban aerosol samples from pollution and non-pollution periods for AA enantiomers determination.We suggest that the concentrations of D-AAs and the ratio of D-AA/L-AA are indicative of the contribution of bacterial sources and the infuence of biomass burning. 展开更多
关键词 amino acid enantiomers Atmospheric aerosol Organic carbon AMMONIA Chiral ratio
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桑枝栽培榆黄蘑子实体的氨基酸组成分析及蛋白质营养价值评价
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作者 谢唯 欧娜 +3 位作者 李志鹏 刘兴胥 方文春 莫秀丽 《中国食用菌》 2024年第3期56-62,共7页
对以桑枝栽培的榆黄蘑子实体中蛋白质、氨基酸含量及相关指标进行分析和评价,检测得到桑枝榆黄蘑子实体蛋白质含量为26.60 g·100^(-1)·g^(-1),属于高蛋白食品;含有18种氨基酸,种类齐全,较接近FAO/WHO模式的标准;支链氨基酸和... 对以桑枝栽培的榆黄蘑子实体中蛋白质、氨基酸含量及相关指标进行分析和评价,检测得到桑枝榆黄蘑子实体蛋白质含量为26.60 g·100^(-1)·g^(-1),属于高蛋白食品;含有18种氨基酸,种类齐全,较接近FAO/WHO模式的标准;支链氨基酸和芳香族氨基酸总量的比值为2.63;经氨基酸评分(amino acid score,AAS)、化学评分(chemical score,CS)、氨基酸比值系数评分(score of ratio coefficient of amino acid,SRCAA)3种指标评分,其第一限制氨基酸均为胱氨酸+蛋氨酸(Cys+Met);以FAO/WHO模式评价,桑枝榆黄蘑子实体所含蛋白质是优质蛋白,且营养价值较高。 展开更多
关键词 榆黄蘑 桑枝栽培 氨基酸组成比例 蛋白质营养价值
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Imbalanced dietary methionine-to-sulfur amino acid ratio can affect amino acid profiles, antioxidant capacity, and intestinal morphology of piglets 被引量:4
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作者 Miaomiao Bai Lei Wang +4 位作者 Hongnan Liu Kang Xu Jinping Deng Ruilin Huang Yulong Yin 《Animal Nutrition》 SCIE 2020年第4期447-456,共10页
Animal protein sources such as fishmeal and plasma powder are excellent and indispensable sources of energy,amino acid s,and minerals in animal production.Amino acid imbalance,especially methionine-to-sulfur amino aci... Animal protein sources such as fishmeal and plasma powder are excellent and indispensable sources of energy,amino acid s,and minerals in animal production.Amino acid imbalance,especially methionine-to-sulfur amino acid(Met:SAA)ratio,caused by an imbalance of animal protein meal leads to growth restriction.This study was conducted to evaluate the effects of imbalanced Met:SAA ratio supplementation of different animal protein source diets on growth performance,plasma amino acid profiles,antioxidant capacity and intestinal morphology in a piglet model.Twenty-four weaned piglets(castrated males;BW=10.46±0.34 kg),assigned randomly into 3 groups(8 piglets/group),were fed for 28 d.Three experimental diets of equal energy and crude protein levels were as follows:1)a corn-soybean basal diet with a Met:SAA ratio at 0.51(BD);2)a plasma powder diet with a low Met:SAA ratio at 0.41(L-MR);3)a fishmeal diet with a high Met:SAA ratio at0.61(H-MR).Results revealed that compared to BD,L-MR significantly decreased(P<0.05)the activities of plasma total antioxidant capacity and glutathione peroxidase,plasma amino acid profiles,and significantly reduced(P<0.05)villus height and crypt depth in the duodenum and jejunum.Additionally,L-MR significantly reduced(P<0.05)the mRNA expression level of solute carrier family 7 member 9(SIC7 A9)in the ileum,and significantly increased(P<0.05)mRNA expression levels of zonula occludens-1(ZO-1)in the duodenum,and Claudin-1,ZO-1,sodium-coupled neutral amino acid transporters 2(SNAT2)and SIC7 A7 in the j ejunum.HMR significantly increased(P<0.05)plasma SAA levels,and significantly reduced(P<0.05)average daily feed intake,villus height,and villus height-to-crypt depth(VH:CD)ratio in the ileum compared to BD.In conclusion,L-MR may result in oxidative stress and villous atrophy but proves beneficial in improving intestinal barrier function and the activity of amino acid transporters for compensatory growth.H-MR may impair intestinal growth and development for weaned piglets.The research provides a guidance on the adequate Met:SAA ratio(0.51)supplementation in diet structure for weaned piglets. 展开更多
关键词 Animal protein source Methionine-to-sulfur amino acid ratio Antioxidant capacity Intestinal morphology PIGLET
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厌氧消化技术在处理有机废弃物方面的应用现状
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作者 王兆轩 敖国旭 +2 位作者 葛菁萍 孙珊珊 凌宏志 《中国农学通报》 2024年第20期84-91,共8页
本研究聚焦于评估厌氧消化技术在处理有机废弃物、转化为再生能源和有机肥料方面的应用,并探讨了其对环境影响的减缓作用。采用文献综述方法,深入分析了温度、碳氮比、有机负荷率、挥发性脂肪酸、水力停留时间和pH等因素如何影响厌氧消... 本研究聚焦于评估厌氧消化技术在处理有机废弃物、转化为再生能源和有机肥料方面的应用,并探讨了其对环境影响的减缓作用。采用文献综述方法,深入分析了温度、碳氮比、有机负荷率、挥发性脂肪酸、水力停留时间和pH等因素如何影响厌氧消化过程的效率与稳定性。研究表明,这些参数对促进微生物活动、加快有机物分解以及维持系统稳定运行均具有显著影响。通过对玉米秸秆、畜禽粪便和厨余垃圾等典型有机废弃物的案例分析,验证了厌氧消化技术的有效性,并详细阐述了该过程中关键微生物群落的结构和功能,包括细菌和古菌在不同阶段如水解、酸化、乙酸化和产甲烷中的作用机制。此外,文章还讨论了有毒物质生成问题及其管理策略。最终,文章建议通过提升厌氧消化效率、开发新型反应器技术以及加强微生物种群研究,将有助于使厌氧消化技术在未来实现更高效、稳定且广泛的运用。 展开更多
关键词 厌氧消化 有机废弃物 资源循环利用 环境污染 微生物群落 有毒物质管理 温度 碳氮比 有机负荷率 挥发性脂肪酸 水力停留时间 pH
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生物体中氨基酸单体碳稳定同位素测试方法研究
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作者 梁建鑫 尹希杰 +2 位作者 苏静 林锡煌 李玉红 《分析测试学报》 CAS CSCD 北大核心 2024年第3期447-454,共8页
氨基酸作为蛋白质的基本组成单位,是重要的生命物质,其单体碳同位素研究在生物地球化学、生态学、生物体代谢和环境科学等领域具有重要意义。该文优化了海参和海藻氨基酸提取和纯化流程,通过N新戊酰基-O-异丙酯(NPP)方法衍生化后,分别... 氨基酸作为蛋白质的基本组成单位,是重要的生命物质,其单体碳同位素研究在生物地球化学、生态学、生物体代谢和环境科学等领域具有重要意义。该文优化了海参和海藻氨基酸提取和纯化流程,通过N新戊酰基-O-异丙酯(NPP)方法衍生化后,分别用气相色谱-质谱(GC-MS)和气相色谱-燃烧-同位素比值质谱(GC-C-IRMS)测试其浓度和碳同位素组成。结果显示,15种氨基酸单体的分离效果较好,回收率为46.4%~96.3%,各氨基酸在1.0~16.0µmol/L范围内线性关系良好(r^(2)为0.987~0.999)。15种氨基酸单体衍生物δ^(13)C值的标准偏差均小于0.30‰(n=10),在0.6~2.0 mmol/L浓度范围内δ^(13)C的平均误差为±0.24‰,方法检出限为0.6 nmol。海参和海藻样品各氨基酸单体δ^(13)C值的范围分别为-31.10‰~-8.58‰和-30.53‰~-13.76‰,标准偏差均在0.33‰以内,可满足生物体氨基酸单体碳同位素的测试精度需求。 展开更多
关键词 气相色谱-燃烧-同位素比值质谱(GC-C-IRMS) N-新戊酰基-O-异丙酯(NPP) 氨基酸 碳稳定同位素
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油菜华油杂62不同生长期氨基酸组成及营养价值评价 被引量:26
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作者 杨雪海 张巍 +5 位作者 赵娜 陈芳 魏金涛 傅廷栋 周广生 李晓峰 《中国油料作物学报》 CAS CSCD 北大核心 2017年第2期197-203,共7页
为明确甘蓝型油菜作为饲料使用的蛋白营养价值,以FAO/WHO提出的人体必需氨基酸模式评价标准,通过氨基酸比值系数法和必需氨基酸指数,评价华油杂62不同生长期植株蛋白质的营养价值。结果表明:华油杂62越冬苗期蛋白质随生长期的延续呈逐... 为明确甘蓝型油菜作为饲料使用的蛋白营养价值,以FAO/WHO提出的人体必需氨基酸模式评价标准,通过氨基酸比值系数法和必需氨基酸指数,评价华油杂62不同生长期植株蛋白质的营养价值。结果表明:华油杂62越冬苗期蛋白质随生长期的延续呈逐渐下降的趋势。不同生长期油菜蛋白质氨基酸种类丰富。必需氨基酸中盛花期蛋氨酸+胱氨酸(3.36%)、苯丙氨酸+酪氨酸(10.11%)的含量和结荚初期的异亮氨酸(5.64%)、亮氨酸(9.45%)以及缬氨酸(6.73%)的含量占总氨基酸的比例高于其它生长期;盛花期和结荚初期的总必需氨基酸/总氨基酸(TEAA/TAA)(44.88%、44.36%)和总必需氨基酸/总非必需氨基酸(0.81、0.80)也高于其它生长期;通过氨基酸比值系数法和必需氨基酸指数评价,油菜盛花期及盛花期后(结荚初期、结荚后期)的必需氨基酸指数均优于其它生长期,盛花期的氨基酸比值系数分值最高达82。以肉牛、绵羊、山羊肉质中的必需氨基酸组成为参考标准,不同生长期油菜的必需氨基酸指数均大于0.95,且油菜盛花期及盛花期后(结荚初期、结荚后期)高于其它生长期,表明盛花期及盛花期后的植株可作为反刍动物的优质蛋白源。 展开更多
关键词 甘蓝型油菜 氨基酸组成 氨基酸比值 必需氨基酸指数 饲草蛋白
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日粮中赖氨酸和蛋氨酸比对断奶犊牛生长性能和消化代谢的影响 被引量:49
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作者 云强 刁其玉 +3 位作者 屠焰 张乃锋 王建红 周盟 《中国农业科学》 CAS CSCD 北大核心 2011年第1期133-142,共10页
【目的】研究日粮中不同赖氨酸和蛋氨酸比例对断奶犊牛生长性能、消化代谢的影响。【方法】选取24头健康的荷斯坦断奶犊牛,按体重和出生时间随机分为4组,限量饲喂4种不同日粮,即对照组日粮(粗蛋白为14.67%),3种赖氨酸和蛋氨酸比例分别为... 【目的】研究日粮中不同赖氨酸和蛋氨酸比例对断奶犊牛生长性能、消化代谢的影响。【方法】选取24头健康的荷斯坦断奶犊牛,按体重和出生时间随机分为4组,限量饲喂4种不同日粮,即对照组日粮(粗蛋白为14.67%),3种赖氨酸和蛋氨酸比例分别为2.5﹕1、3.0﹕1和3.5﹕1的日粮(粗蛋白为12.02%),试验期为8周。在试验开始和结束时对犊牛进行称重和采血,在试验第3周每组选取4头犊牛采用全收粪、尿法进行代谢试验。【结果】日粮中赖蛋比为3.0﹕1组的犊牛增重在数值上要高于其它3组(P>0.05)。3个试验组犊牛血清尿素氮浓度要显著低于对照组(P<0.01),游离氨基酸浓度在数值上要低于对照组(蛋氨酸除外)(P>0.05)。赖蛋比对血清尿素氮和游离氨基酸浓度无显著影响(精氨酸、缬氨酸除外)。赖蛋氨酸比为3.0﹕1组和3.5﹕1组犊牛氮的总利用率和表观生物学价值在数值上要高于2.5﹕1组和对照组(P>0.05)。【结论】在粗蛋白含量为12.02%的日粮中添加过瘤胃赖氨酸和过瘤胃蛋氨酸,犊牛的增重可超过粗蛋白为14.67%日粮的水平。赖氨酸和蛋氨酸比例为3.0﹕1有利于犊牛生长,并可提高氮的利用效率。 展开更多
关键词 犊牛 氨基酸比例 生长性能 消化代谢
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黄伞菌丝蛋白质营养价值评价 被引量:18
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作者 姜华 黄清荣 +2 位作者 臧丽丽 高成彬 蔡德华 《菌物学报》 CAS CSCD 北大核心 2005年第3期441-445,共5页
本文测定了黄伞Pholiotaadiposa菌丝粗蛋白含量和氨基酸组成,采用国际上通用的营养价值评价方法,对其蛋白质的营养价值进行全面评价,并与营养价值较高的白灵侧耳Pleurotusnebrodensis、刺芹侧耳Pleurotuseryngii和金顶侧耳Pleurotrscitr... 本文测定了黄伞Pholiotaadiposa菌丝粗蛋白含量和氨基酸组成,采用国际上通用的营养价值评价方法,对其蛋白质的营养价值进行全面评价,并与营养价值较高的白灵侧耳Pleurotusnebrodensis、刺芹侧耳Pleurotuseryngii和金顶侧耳Pleurotrscitrinopileatus进行比较。分析结果表明,黄伞菌丝的必需氨基酸含量最高,占其氨基酸总量的43.9%,蛋白质的氨基酸评分(AAS)、化学评分(CS)、必需氨基酸指数(EAAI)、生物价(BV)、营养指数(NI)和氨基酸比值系数分(SRCAA)分别为92.0、71.3、88.5、84.8、40.4和78.8,六项指标均比参比食用菌高。结果说明黄伞菌丝具有很高的营养价值。 展开更多
关键词 氨基酸评分 化学评分 必需氨基酸指数 生物价 营养指数 氮基酸比值系数分
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