ObjectiveThe aim was to assess genetic and physiological toxicity of wastewater from a pharmaceutical factory using root tip micronucleus technology of Vicia faba. MethodThe pollution of wastewater from a pharmaceutic...ObjectiveThe aim was to assess genetic and physiological toxicity of wastewater from a pharmaceutical factory using root tip micronucleus technology of Vicia faba. MethodThe pollution of wastewater from a pharmaceutical factory was detected by using root tip micronucleus technology of Vicia faba, and the genetic and physiological toxicity of the wastewater to Vicia faba was assessed. ResultNon-processed wastewater had an extremely high level of biological toxicity; the cells were unable to live with the wastewater at a high concentration; the cells were able to grow with the wastewater at a low concentration, though the micronucleus ratio was extremely high. The processed wastewater had no significant impact on cell growth, but the micronucleus ratio was extremely high, showing that the processed water also had a high pollution index. ConclusionThe research could provide scientific references for the national treatment of wastewater from a pharmaceutical factory.展开更多
[Objectives]This study was conducted to analyze the genetic toxicity of Solidago decurrens,with a view to scientifically and safely using S.decurrens.[Methods]Using commercially available S.decurrens as an experimenta...[Objectives]This study was conducted to analyze the genetic toxicity of Solidago decurrens,with a view to scientifically and safely using S.decurrens.[Methods]Using commercially available S.decurrens as an experimental material,according to Vicia faba root tip micronucleus technique,V.faba root tips were treated with different doses of aqueous extract from S.decurrens for different times,and the mitotic index,frequency of micronucleus and chromosomal aberration of root tips in V.faba were detected,so as to reveal the allelopathic potential and the potential ecological risks of S.decurrens at the cellular level.[Results]The decrease in the mitotic index caused by the aqueous extract of S.decurrens was positively related to the concentration of the aqueous extract and the treatment time.The aqueous extract of S.decurrens increased the frequency of micronucleus in root tip cells of V.faba,and the increase was positively related to the concentration of the aqueous extract and the treatment time.The aqueous extract of S.decurrens caused abnormal phenomena such as chromosome bridges,chromosome fragmentation,and chromosome lag in V.faba root tip cells,and the effect of the aqueous extract on V.faba root tips had significant effects dependent on time and concentration.Above results indicate that the aqueous extract of S.decurrens inhibited and damaged the mitosis of V.faba root tip cells to some degrees,had certain genetic toxicity and had the ability to rapidly spread.[Conclusions]This study provides a theoretical basis for scientifically and rationally using S.decurrens and preventing the spread of S.decurrens.展开更多
文摘ObjectiveThe aim was to assess genetic and physiological toxicity of wastewater from a pharmaceutical factory using root tip micronucleus technology of Vicia faba. MethodThe pollution of wastewater from a pharmaceutical factory was detected by using root tip micronucleus technology of Vicia faba, and the genetic and physiological toxicity of the wastewater to Vicia faba was assessed. ResultNon-processed wastewater had an extremely high level of biological toxicity; the cells were unable to live with the wastewater at a high concentration; the cells were able to grow with the wastewater at a low concentration, though the micronucleus ratio was extremely high. The processed wastewater had no significant impact on cell growth, but the micronucleus ratio was extremely high, showing that the processed water also had a high pollution index. ConclusionThe research could provide scientific references for the national treatment of wastewater from a pharmaceutical factory.
基金Innovation Center Team Project of Hubei Collaborative Innovation Center for the Characteristic Resources Exploration of Dabie Mountains(2015TD07)。
文摘[Objectives]This study was conducted to analyze the genetic toxicity of Solidago decurrens,with a view to scientifically and safely using S.decurrens.[Methods]Using commercially available S.decurrens as an experimental material,according to Vicia faba root tip micronucleus technique,V.faba root tips were treated with different doses of aqueous extract from S.decurrens for different times,and the mitotic index,frequency of micronucleus and chromosomal aberration of root tips in V.faba were detected,so as to reveal the allelopathic potential and the potential ecological risks of S.decurrens at the cellular level.[Results]The decrease in the mitotic index caused by the aqueous extract of S.decurrens was positively related to the concentration of the aqueous extract and the treatment time.The aqueous extract of S.decurrens increased the frequency of micronucleus in root tip cells of V.faba,and the increase was positively related to the concentration of the aqueous extract and the treatment time.The aqueous extract of S.decurrens caused abnormal phenomena such as chromosome bridges,chromosome fragmentation,and chromosome lag in V.faba root tip cells,and the effect of the aqueous extract on V.faba root tips had significant effects dependent on time and concentration.Above results indicate that the aqueous extract of S.decurrens inhibited and damaged the mitosis of V.faba root tip cells to some degrees,had certain genetic toxicity and had the ability to rapidly spread.[Conclusions]This study provides a theoretical basis for scientifically and rationally using S.decurrens and preventing the spread of S.decurrens.
