[ Objectives ] The aim was to optimize the configuration of seedling density and line spacing of forage sweet sorghum ( Sorghum blcolor ( L. ) Moench) and explore its high-yield cultivation techniques. [ Methods] ...[ Objectives ] The aim was to optimize the configuration of seedling density and line spacing of forage sweet sorghum ( Sorghum blcolor ( L. ) Moench) and explore its high-yield cultivation techniques. [ Methods] Effects of such two influencing factors as line spacing and seedling density on the leaf-stem ratio, DW/FW ratio and grass yield of forage sweet sorghum were analyzed by using split-plot experiment design experiment method and LSD method of IBM. SPSS. Statis- tics. v22 stati, stics software. [ Results ~ Seedling density and line spacing had no obvious effect on the leaf-stem ratio and DW/FW ratio of forage sweet sorghum but had obvious influences on the grass yield. Moreover, the optimal combination of seedling density and line spacing for high yield of forage sweet sorghum was A2 B4, that is, seedling density was 225 000 plants/hm2, and line spacing was 40 cm. [ Conclusions] The results provided a theoretical basis and technical support for high-yield cultivation techniques of forage sweet sorghum.展开更多
A novel environmental friendly wheat seed coating agent was prepared from the natural polymer,biological pesticide,microfertilizer,microelements and other additives.The laboratory and field test results showed that it...A novel environmental friendly wheat seed coating agent was prepared from the natural polymer,biological pesticide,microfertilizer,microelements and other additives.The laboratory and field test results showed that it could enhance the yield up to 16% and reduce its input cost to 1/6 as compared with the conventional wheat seed coating agent.It is safe without causing any pollution and would have significant economic and environmental benefits.展开更多
In order to screen suitable high hybrid wheat seed production technology,the split-plot experiment design was adopted and study was carried out about the effects of the different row ratios of male and female parents ...In order to screen suitable high hybrid wheat seed production technology,the split-plot experiment design was adopted and study was carried out about the effects of the different row ratios of male and female parents and application amount of nitrogen fertilizer on hybrid wheat seed production and its component factors. The results showed that the seed production increased with the increase in the number of female parent row. When the row ratio of male and female parents was 2 ∶ 6,the seed production was 3 683. 8 kg/ha; when the application amount of nitrogen fertilization was 50 kg/ha,the seed production was 3 649. 4 kg/ha; the interaction between the row ratio of male and female parents and the application amount of nitrogen fertilizer indicated that when the row ratio of male and female parents was 2∶ 6 and the application amount of nitrogen fertilizer was 300 kg/ha,the seed production reached the highest( 4160. 6 kg/ha). The row ratio of male and female parents and application amount of nitrogen fertilizer had significant effect on the component factors of seed production,including the number of grains per spike,spike weight and setting percentage. When the row ratio of male and female parents was 2∶ 5,the number of grains per spike,spike weight and setting percentage were the highest at 26. 7 grains,1. 12 g,and 62. 6% respectively; when application amount of nitrogen fertilizer was 450 kg/ha,the number of grains per spike,spike weight and setting percentage were the highest at 26. 0 grains,1. 08 g,and59. 2% respectively; the interaction of row ratio of male and female parents and application amount of nitrogen fertilizer had significant effect on the number of grains per spike,spike weight and setting percentage; when the row ratio of male and female parents was 2∶ 5 and the application amount of nitrogen fertilizer was 300 kg/ha,the number of grains per spike,spike weight and setting percentage were the highest at 29. 6grains,1. 24 g,and 71. 6% respectively. The number of grains per spike is the largest component factor for seed production. Increasing the number of grains per spike can increase the seed production. According to the effects of row ratio of male and female parents and application amount of nitrogen fertilizer on the component factors of seed production,the optimal condition was 2∶ 5-2∶ 6 for row ratio of male and female parents and 300-450 kg/ha for application amount of nitrogen fertilizer.展开更多
It was hypothesized that mesenchymal stem cells(MSCs) could provide necessary trophic factors when seeded onto the surfaces of commonly used nerve graft substitutes. We aimed to determine the gene expression of MSCs w...It was hypothesized that mesenchymal stem cells(MSCs) could provide necessary trophic factors when seeded onto the surfaces of commonly used nerve graft substitutes. We aimed to determine the gene expression of MSCs when influenced by Avance■ Nerve Grafts or Neura Gen■ Nerve Guides. Human adipose-derived MSCs were cultured and dynamically seeded onto 30 Avance■ Nerve Grafts and 30 Neura Gen■ Nerve Guides for 12 hours. At six time points after seeding, quantitative polymerase chain reaction analyses were performed for five samples per group. Neurotrophic [nerve growth factor(NGF), glial cell line-derived neurotrophic factor(GDNF), pleiotrophin(PTN), growth associated protein 43(GAP43) and brain-derived neurotrophic factor(BDNF)], myelination [peripheral myelin protein 22(PMP22) and myelin protein zero(MPZ)], angiogenic [platelet endothelial cell adhesion molecule 1(PECAM1/CD31) and vascular endothelial cell growth factor alpha(VEGFA)], extracellular matrix(ECM) [collagen type alpha I(COL1A1), collagen type alpha III(COL3A1), Fibulin 1(FBLN1) and laminin subunit beta 2(LAMB2)] and cell surface marker cluster of differentiation 96(CD96) gene expression was quantified. Unseeded Avance■ Nerve Grafts and Neura Gen■ Nerve Guides were used to evaluate the baseline gene expression, and unseeded MSCs provided the baseline gene expression of MSCs. The interaction of MSCs with the Avance■ Nerve Grafts led to a short-term upregulation of neurotrophic(NGF, GDNF and BDNF), myelination(PMP22 and MPZ) and angiogenic genes(CD31 and VEGFA) and a long-term upregulation of BDNF, VEGFA and COL1A1. The interaction between MSCs and the Neura Gen■ Nerve Guide led to short term upregulation of neurotrophic(NGF, GDNF and BDNF) myelination(PMP22 and MPZ), angiogenic(CD31 and VEGFA), ECM(COL1A1) and cell surface(CD96) genes and long-term upregulation of neurotrophic(GDNF and BDNF), angiogenic(CD31 and VEGFA), ECM genes(COL1A1, COL3A1, and FBLN1) and cell surface(CD96) genes. Analysis demonstrated MSCs seeded onto Neura Gen■ Nerve Guides expressed significantly higher levels of neurotrophic(PTN), angiogenic(VEGFA) and ECM(COL3A1, FBLN1) genes in the long term period compared to MSCs seeded onto Avance■ Nerve Grafts. Overall, the interaction between human MSCs and both nerve graft substitutes resulted in a significant upregulation of the expression of numerous genes important for nerve regeneration over time. The in vitro interaction of MSCs with the Neura Gen■ Nerve Guide was more pronounced, particularly in the long term period(> 14 days after seeding). These results suggest that MSC-seeding has potential to be applied in a clinical setting, which needs to be confirmed in future in vitro and in vivo research.展开更多
Maize is widely planted throughout the world and has the highest yield of all the cereal crops. The arid region of North- west China has become the largest base for seed-maize production, but water shortage is the bot...Maize is widely planted throughout the world and has the highest yield of all the cereal crops. The arid region of North- west China has become the largest base for seed-maize production, but water shortage is the bottleneck for its long-term sustainability. Investigating the transpiration of seed-maize plants will offer valuable information for suitable planting and irrigation strategies in this arid area. In this study, stem flow was measured using a heat balance method under alternate furrow irrigation and double-row ridge planting. Meteorological factors, soil water content (e), soil temperature (Ts) and leaf area (LA) were also monitored during 2012 and 2013. The diurnal stem flow and seasonal dynamics of maize plants in the zones of south side female parent (SFP), north side female parent (NFP) and male parent (MP) were investigated. The order of stem flow rate was: SFP〉MP〉NFP. The relationships between stem flow and influential factors during three growth stages at different time scales were analyzed. On an hourly scale, solar radiation (Rs) was the main driving factor of stem flow. The influence of air temperature (Ta) during the maturity stage was significantly higher than in other periods. On a daily scale, Rs was the main driving factor of stem flow during the heading stage. During the filling growth stage, the main driving factor of NFP and MP stem flow was RH and Ts, respectively. However, during the maturity stage, the environ- mental factors had no significant influence on seed-maize stem flow. For different seed-maize plants, the main influential factors were different in each of the three growing seasons. Therefore, we identified them to accurately model the FP and MP stem flow and applied precision irrigation under alternate partial root-zone furrow irrigation to analyze major factors affecting stem flow in different scales.展开更多
Field experiments were conducted during 2008-2010 to investigate the effects of NPK fertilization on seed yield and economic benefit of a new hybrid cultivar of winter oilseed rape(Brassica napus L.)- Xiangzayou763,an...Field experiments were conducted during 2008-2010 to investigate the effects of NPK fertilization on seed yield and economic benefit of a new hybrid cultivar of winter oilseed rape(Brassica napus L.)- Xiangzayou763,and to determine the optimum NPK ratio and dose in Hunan,China.The results showed that NPK ratio(1:0.50:0.50) with 180 kg/hm2 N,90 kg/hm^2 P_2O_5 and 90 kg/hm^2 K_2O was the optimum combination for the highest seed yield(2 231.13 kg/hm2) and economic benefit(9 816.97 Yuan/hm^2),of which 1 641.23 Yuan/hm^2 being set for fertilizer investment(VCR=4.11).Besides,the effects of N,P and K fertilizers on seed yield and oil production of winter oilseed rape were not identical.N had the greatest effect on plant growth of winter oilseed rape,followed by P and K.Total leaf number,green leaf number,leaf length and width,rhizome diameter,branch number,dry weight per plant and other agronomic characters in high N treatments were higher than those in low N treatments.By correlation and path analysis of yield components,the contribution rate to seed yield was found:effective silique number per plant>seed number per silique>1 000-seed weight.展开更多
基金Supported by Special Fund for Agro-scientific Research in the Public Interest(20120304201)
文摘[ Objectives ] The aim was to optimize the configuration of seedling density and line spacing of forage sweet sorghum ( Sorghum blcolor ( L. ) Moench) and explore its high-yield cultivation techniques. [ Methods] Effects of such two influencing factors as line spacing and seedling density on the leaf-stem ratio, DW/FW ratio and grass yield of forage sweet sorghum were analyzed by using split-plot experiment design experiment method and LSD method of IBM. SPSS. Statis- tics. v22 stati, stics software. [ Results ~ Seedling density and line spacing had no obvious effect on the leaf-stem ratio and DW/FW ratio of forage sweet sorghum but had obvious influences on the grass yield. Moreover, the optimal combination of seedling density and line spacing for high yield of forage sweet sorghum was A2 B4, that is, seedling density was 225 000 plants/hm2, and line spacing was 40 cm. [ Conclusions] The results provided a theoretical basis and technical support for high-yield cultivation techniques of forage sweet sorghum.
基金supported by the Scientific and Tech-nological Project of Zhejiang Province,China (ZK-20061103)
文摘A novel environmental friendly wheat seed coating agent was prepared from the natural polymer,biological pesticide,microfertilizer,microelements and other additives.The laboratory and field test results showed that it could enhance the yield up to 16% and reduce its input cost to 1/6 as compared with the conventional wheat seed coating agent.It is safe without causing any pollution and would have significant economic and environmental benefits.
基金Supported by State Key Research and Development Program of Ministry of Science and Technology(2016YFD0101603)Fundamental Research Funds for the Central Universities in 2016(XDJK2016A020)
文摘In order to screen suitable high hybrid wheat seed production technology,the split-plot experiment design was adopted and study was carried out about the effects of the different row ratios of male and female parents and application amount of nitrogen fertilizer on hybrid wheat seed production and its component factors. The results showed that the seed production increased with the increase in the number of female parent row. When the row ratio of male and female parents was 2 ∶ 6,the seed production was 3 683. 8 kg/ha; when the application amount of nitrogen fertilization was 50 kg/ha,the seed production was 3 649. 4 kg/ha; the interaction between the row ratio of male and female parents and the application amount of nitrogen fertilizer indicated that when the row ratio of male and female parents was 2∶ 6 and the application amount of nitrogen fertilizer was 300 kg/ha,the seed production reached the highest( 4160. 6 kg/ha). The row ratio of male and female parents and application amount of nitrogen fertilizer had significant effect on the component factors of seed production,including the number of grains per spike,spike weight and setting percentage. When the row ratio of male and female parents was 2∶ 5,the number of grains per spike,spike weight and setting percentage were the highest at 26. 7 grains,1. 12 g,and 62. 6% respectively; when application amount of nitrogen fertilizer was 450 kg/ha,the number of grains per spike,spike weight and setting percentage were the highest at 26. 0 grains,1. 08 g,and59. 2% respectively; the interaction of row ratio of male and female parents and application amount of nitrogen fertilizer had significant effect on the number of grains per spike,spike weight and setting percentage; when the row ratio of male and female parents was 2∶ 5 and the application amount of nitrogen fertilizer was 300 kg/ha,the number of grains per spike,spike weight and setting percentage were the highest at 29. 6grains,1. 24 g,and 71. 6% respectively. The number of grains per spike is the largest component factor for seed production. Increasing the number of grains per spike can increase the seed production. According to the effects of row ratio of male and female parents and application amount of nitrogen fertilizer on the component factors of seed production,the optimal condition was 2∶ 5-2∶ 6 for row ratio of male and female parents and 300-450 kg/ha for application amount of nitrogen fertilizer.
基金supported by the National Institute of Neurological Disorders and Stroke of the National Institutes of Health (No. R01NS102360)。
文摘It was hypothesized that mesenchymal stem cells(MSCs) could provide necessary trophic factors when seeded onto the surfaces of commonly used nerve graft substitutes. We aimed to determine the gene expression of MSCs when influenced by Avance■ Nerve Grafts or Neura Gen■ Nerve Guides. Human adipose-derived MSCs were cultured and dynamically seeded onto 30 Avance■ Nerve Grafts and 30 Neura Gen■ Nerve Guides for 12 hours. At six time points after seeding, quantitative polymerase chain reaction analyses were performed for five samples per group. Neurotrophic [nerve growth factor(NGF), glial cell line-derived neurotrophic factor(GDNF), pleiotrophin(PTN), growth associated protein 43(GAP43) and brain-derived neurotrophic factor(BDNF)], myelination [peripheral myelin protein 22(PMP22) and myelin protein zero(MPZ)], angiogenic [platelet endothelial cell adhesion molecule 1(PECAM1/CD31) and vascular endothelial cell growth factor alpha(VEGFA)], extracellular matrix(ECM) [collagen type alpha I(COL1A1), collagen type alpha III(COL3A1), Fibulin 1(FBLN1) and laminin subunit beta 2(LAMB2)] and cell surface marker cluster of differentiation 96(CD96) gene expression was quantified. Unseeded Avance■ Nerve Grafts and Neura Gen■ Nerve Guides were used to evaluate the baseline gene expression, and unseeded MSCs provided the baseline gene expression of MSCs. The interaction of MSCs with the Avance■ Nerve Grafts led to a short-term upregulation of neurotrophic(NGF, GDNF and BDNF), myelination(PMP22 and MPZ) and angiogenic genes(CD31 and VEGFA) and a long-term upregulation of BDNF, VEGFA and COL1A1. The interaction between MSCs and the Neura Gen■ Nerve Guide led to short term upregulation of neurotrophic(NGF, GDNF and BDNF) myelination(PMP22 and MPZ), angiogenic(CD31 and VEGFA), ECM(COL1A1) and cell surface(CD96) genes and long-term upregulation of neurotrophic(GDNF and BDNF), angiogenic(CD31 and VEGFA), ECM genes(COL1A1, COL3A1, and FBLN1) and cell surface(CD96) genes. Analysis demonstrated MSCs seeded onto Neura Gen■ Nerve Guides expressed significantly higher levels of neurotrophic(PTN), angiogenic(VEGFA) and ECM(COL3A1, FBLN1) genes in the long term period compared to MSCs seeded onto Avance■ Nerve Grafts. Overall, the interaction between human MSCs and both nerve graft substitutes resulted in a significant upregulation of the expression of numerous genes important for nerve regeneration over time. The in vitro interaction of MSCs with the Neura Gen■ Nerve Guide was more pronounced, particularly in the long term period(> 14 days after seeding). These results suggest that MSC-seeding has potential to be applied in a clinical setting, which needs to be confirmed in future in vitro and in vivo research.
基金grants from the National Natural Science Foundation of China (51222905, 51321001, 51439006)the National High-Tech R&D Program of China (863 Program, 2011AA100505)+1 种基金the Ministry of Water Resources of China (201201003)the Program for New Century Excellent Talents in University, Ministry of Education, China (NCET11-0479)
文摘Maize is widely planted throughout the world and has the highest yield of all the cereal crops. The arid region of North- west China has become the largest base for seed-maize production, but water shortage is the bottleneck for its long-term sustainability. Investigating the transpiration of seed-maize plants will offer valuable information for suitable planting and irrigation strategies in this arid area. In this study, stem flow was measured using a heat balance method under alternate furrow irrigation and double-row ridge planting. Meteorological factors, soil water content (e), soil temperature (Ts) and leaf area (LA) were also monitored during 2012 and 2013. The diurnal stem flow and seasonal dynamics of maize plants in the zones of south side female parent (SFP), north side female parent (NFP) and male parent (MP) were investigated. The order of stem flow rate was: SFP〉MP〉NFP. The relationships between stem flow and influential factors during three growth stages at different time scales were analyzed. On an hourly scale, solar radiation (Rs) was the main driving factor of stem flow. The influence of air temperature (Ta) during the maturity stage was significantly higher than in other periods. On a daily scale, Rs was the main driving factor of stem flow during the heading stage. During the filling growth stage, the main driving factor of NFP and MP stem flow was RH and Ts, respectively. However, during the maturity stage, the environ- mental factors had no significant influence on seed-maize stem flow. For different seed-maize plants, the main influential factors were different in each of the three growing seasons. Therefore, we identified them to accurately model the FP and MP stem flow and applied precision irrigation under alternate partial root-zone furrow irrigation to analyze major factors affecting stem flow in different scales.
基金Supported by the Youth Fund of Orient Science and Technology College,Hunan Agricultural University(14QNZ09)Cultivation Physiology Station of National Technical System in Rape Industrial,National Key Technology Support Program(2012BAD15B04,2010BAD01B01)+1 种基金Special Fund of Government in Hunan Province,National Natural Science Foundation of China(31071851,31101596,3132130)Open Fund Project of Innovation Platform in Colleges and Universities of Hunan Province(12K064)
文摘Field experiments were conducted during 2008-2010 to investigate the effects of NPK fertilization on seed yield and economic benefit of a new hybrid cultivar of winter oilseed rape(Brassica napus L.)- Xiangzayou763,and to determine the optimum NPK ratio and dose in Hunan,China.The results showed that NPK ratio(1:0.50:0.50) with 180 kg/hm2 N,90 kg/hm^2 P_2O_5 and 90 kg/hm^2 K_2O was the optimum combination for the highest seed yield(2 231.13 kg/hm2) and economic benefit(9 816.97 Yuan/hm^2),of which 1 641.23 Yuan/hm^2 being set for fertilizer investment(VCR=4.11).Besides,the effects of N,P and K fertilizers on seed yield and oil production of winter oilseed rape were not identical.N had the greatest effect on plant growth of winter oilseed rape,followed by P and K.Total leaf number,green leaf number,leaf length and width,rhizome diameter,branch number,dry weight per plant and other agronomic characters in high N treatments were higher than those in low N treatments.By correlation and path analysis of yield components,the contribution rate to seed yield was found:effective silique number per plant>seed number per silique>1 000-seed weight.