Integration of water, fertilizer and pesticide is the final stage of agricultural development, which improves the utilization efficiency of water, fertilizer and pesticide. Starting from the design and realization of ...Integration of water, fertilizer and pesticide is the final stage of agricultural development, which improves the utilization efficiency of water, fertilizer and pesticide. Starting from the design and realization of water, fertilizer and pesticide integrated automatic control device, the paper discusses selection and application of fertilizer pesticides, use procedure, water, fertilizer and pesticide saving effect and receptive crowd in the application process of modern planting industry, so as to provide the basis for promotion and popularization of water, fertilizer and pesticide integration.展开更多
The selection of the most motile and functionally competent sperm is an essential basis for in vitro fertilization(IVF)and normal embryonic development.Widely adopted clinical approaches for sperm sample processing in...The selection of the most motile and functionally competent sperm is an essential basis for in vitro fertilization(IVF)and normal embryonic development.Widely adopted clinical approaches for sperm sample processing intensely rely on centrifugation and wash steps that may induce mechanical damage and oxidative stress to sperm.Although a few microfluidic sperm sorting devices may avoid these adverse effects by exploiting intrinsic guidance mechanisms of sperm swimming,none of these approaches have been fully validated by clinical-grade assessment criteria.In this study,a microfluidic sperm sorting device that enables the selection of highly motile and functional sperm via their intrinsic thermotaxis is presented.Bioinspired by the temperature microenvironment in the fallopian tube during natural sperm selection,a microfluidic device with controllable temperature gradients along the sperm separation channel was designed and fabricated.This study investigated the optimal temperature conditions for human sperm selection and fully characterized thermotaxis-selected sperm with 45 human sperm samples.Results indicated that a temperature range of 35–36.5℃along the separation channel significantly improves human sperm motility rate((85.25±6.28)%vs.(60.72±1.37)%;P=0.0484),increases normal sperm morphology rate((16.42±1.43)%vs.(12.55±0.88)%;P<0.0001),and reduces DNA fragmentation((7.44±0.79)%vs.(10.36±0.72)%;P=0.0485)compared to the nonthermotaxis group.Sperm thermotaxis is species-specific,and selected mouse sperm displayed the highest motility in response to a temperature range of 36–37.5℃ along the separation channel.Furthermore,IVF experiments indicated that the selected sperm permitted an increased fertilization rate and improved embryonic development from zygote to blastocyst.This microfluidic thermotaxic selection approach will be translated into clinical practice to improve the IVF success rate for patients with oligozoospermia and asthenozoospermia.展开更多
In accordance with current problems such as odor pollution,long production period,consumption of a large amount of manpower and so on existing in chicken manure fermentation ,an environment friendly type of chicken ma...In accordance with current problems such as odor pollution,long production period,consumption of a large amount of manpower and so on existing in chicken manure fermentation ,an environment friendly type of chicken manure fermentation device is designed. The key parameters of the equipment are resonance activated tube and fermenting cellar. Resonance activated tube makes the activated water,which could improve obvi- ously the biodegradation rate of chicken manure. The aerator could provide enough oxygen to microbes,and the agitator could make oxygen evenly distributed across the chicken manure in fermenting cellar. The test shows that this equipment is high-efficiency,and only takes 7 days to make the chicken manure achieve the decomposed requirement. The organic matter content of composted chicken manure is 91.2%,C/N ratio is 18.8, MPN is 0.04, mortality of ascarid egg is 99.6% ,concentrations of NH3 and H2 S are 4.20 and 1.58 mg/m3, furthermore ,there is no offensive smell in the fermenting cellar. Therefore,the study can provide a pollution-free, high-efficiency, manpower saving fermentation equipment for organic fertilizer production by chicken manure in agriculture area.展开更多
Fertilizer sphericity is an important assessment index of appearance quality that affects the fertilization effect.A fertilizer sphericity measuring device based on machine vision was designed aimed at low precision a...Fertilizer sphericity is an important assessment index of appearance quality that affects the fertilization effect.A fertilizer sphericity measuring device based on machine vision was designed aimed at low precision and heavy workload of manual fertilizer measurement,and high cost and complicated operation of high precision measuring instruments.A fertilizer sphericity measuring method based on equatorial and meridian circles was proposed.The device works in an intermittent static acquisition mode to simultaneously obtain both top and side images of a single fertilizer.First,the method performs gamma correction on the top and side images of the single fertilizer,and uses the Canny operator to detect the edge of the image to obtain the equatorial and meridian circular contour images of the fertilizer.Second,based on the fertilizer equatorial and meridian circular contour,the Least Squares Circle method was used to evaluate the roundness of the single fertilizer.Finally,the average roundness value of the equatorial and meridian circles was used as the final sphericity value of the fertilizer.The sphericity measurement test was carried out on the same batch of compound,organic and biological fertilizers by using the sphericity measuring device.The fertilizer sphericity data were obtained by different measurement and evaluation methods.The variation coefficient was used to evaluate the difference in fertilizer sphericity measured by different sphericity measurement and evaluation methods.The results show that among the different measurement and evaluation methods,the coefficient of variation of fertilizer sphericity measured by the equatorial and meridian circle method was the smallest,and the coefficient of variation of sphericity measured by the Least Squares Circle method was the smallest and accurate.This study shows that the sphericity measuring device and method can accurately measure the fertilizer sphericity,and has a significant potential to facilitate fertilizer production and quality inspection.展开更多
基金Supported by Science and Technology Program of Guangxi Province(GK AD19245169,GK AD18281072,GK AA17202037,GK AB16380164)。
文摘Integration of water, fertilizer and pesticide is the final stage of agricultural development, which improves the utilization efficiency of water, fertilizer and pesticide. Starting from the design and realization of water, fertilizer and pesticide integrated automatic control device, the paper discusses selection and application of fertilizer pesticides, use procedure, water, fertilizer and pesticide saving effect and receptive crowd in the application process of modern planting industry, so as to provide the basis for promotion and popularization of water, fertilizer and pesticide integration.
基金supported by the Key Research and Development Project of Hubei Province,China(No.2021BCA111)。
文摘The selection of the most motile and functionally competent sperm is an essential basis for in vitro fertilization(IVF)and normal embryonic development.Widely adopted clinical approaches for sperm sample processing intensely rely on centrifugation and wash steps that may induce mechanical damage and oxidative stress to sperm.Although a few microfluidic sperm sorting devices may avoid these adverse effects by exploiting intrinsic guidance mechanisms of sperm swimming,none of these approaches have been fully validated by clinical-grade assessment criteria.In this study,a microfluidic sperm sorting device that enables the selection of highly motile and functional sperm via their intrinsic thermotaxis is presented.Bioinspired by the temperature microenvironment in the fallopian tube during natural sperm selection,a microfluidic device with controllable temperature gradients along the sperm separation channel was designed and fabricated.This study investigated the optimal temperature conditions for human sperm selection and fully characterized thermotaxis-selected sperm with 45 human sperm samples.Results indicated that a temperature range of 35–36.5℃along the separation channel significantly improves human sperm motility rate((85.25±6.28)%vs.(60.72±1.37)%;P=0.0484),increases normal sperm morphology rate((16.42±1.43)%vs.(12.55±0.88)%;P<0.0001),and reduces DNA fragmentation((7.44±0.79)%vs.(10.36±0.72)%;P=0.0485)compared to the nonthermotaxis group.Sperm thermotaxis is species-specific,and selected mouse sperm displayed the highest motility in response to a temperature range of 36–37.5℃ along the separation channel.Furthermore,IVF experiments indicated that the selected sperm permitted an increased fertilization rate and improved embryonic development from zygote to blastocyst.This microfluidic thermotaxic selection approach will be translated into clinical practice to improve the IVF success rate for patients with oligozoospermia and asthenozoospermia.
基金Supported by National Table Poultry Industry Technology System ItemChina(CARS-42-G21)+1 种基金Technique Innovation Item of Jinan Colleges and AcademiesChina(201302053)
文摘In accordance with current problems such as odor pollution,long production period,consumption of a large amount of manpower and so on existing in chicken manure fermentation ,an environment friendly type of chicken manure fermentation device is designed. The key parameters of the equipment are resonance activated tube and fermenting cellar. Resonance activated tube makes the activated water,which could improve obvi- ously the biodegradation rate of chicken manure. The aerator could provide enough oxygen to microbes,and the agitator could make oxygen evenly distributed across the chicken manure in fermenting cellar. The test shows that this equipment is high-efficiency,and only takes 7 days to make the chicken manure achieve the decomposed requirement. The organic matter content of composted chicken manure is 91.2%,C/N ratio is 18.8, MPN is 0.04, mortality of ascarid egg is 99.6% ,concentrations of NH3 and H2 S are 4.20 and 1.58 mg/m3, furthermore ,there is no offensive smell in the fermenting cellar. Therefore,the study can provide a pollution-free, high-efficiency, manpower saving fermentation equipment for organic fertilizer production by chicken manure in agriculture area.
基金This work was supported in part by the National Key Research and Development Plan of China(2016YFD0201104),National Apple Industry Technology System Project.
文摘Fertilizer sphericity is an important assessment index of appearance quality that affects the fertilization effect.A fertilizer sphericity measuring device based on machine vision was designed aimed at low precision and heavy workload of manual fertilizer measurement,and high cost and complicated operation of high precision measuring instruments.A fertilizer sphericity measuring method based on equatorial and meridian circles was proposed.The device works in an intermittent static acquisition mode to simultaneously obtain both top and side images of a single fertilizer.First,the method performs gamma correction on the top and side images of the single fertilizer,and uses the Canny operator to detect the edge of the image to obtain the equatorial and meridian circular contour images of the fertilizer.Second,based on the fertilizer equatorial and meridian circular contour,the Least Squares Circle method was used to evaluate the roundness of the single fertilizer.Finally,the average roundness value of the equatorial and meridian circles was used as the final sphericity value of the fertilizer.The sphericity measurement test was carried out on the same batch of compound,organic and biological fertilizers by using the sphericity measuring device.The fertilizer sphericity data were obtained by different measurement and evaluation methods.The variation coefficient was used to evaluate the difference in fertilizer sphericity measured by different sphericity measurement and evaluation methods.The results show that among the different measurement and evaluation methods,the coefficient of variation of fertilizer sphericity measured by the equatorial and meridian circle method was the smallest,and the coefficient of variation of sphericity measured by the Least Squares Circle method was the smallest and accurate.This study shows that the sphericity measuring device and method can accurately measure the fertilizer sphericity,and has a significant potential to facilitate fertilizer production and quality inspection.
