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Parental Psychological Control and Internet Gaming Disorder Tendency:A Moderated Mediation Model of Core Self-Evaluation and Intentional Self-Regulation
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作者 Zhiqiao Ji Shuhua Wei Hejuan Ding 《International Journal of Mental Health Promotion》 2024年第7期547-558,共12页
Internet gaming disorder(IGD)among junior high school students is an increasingly prominent mental health concern.It is important to look for influences behind internet gaming disorder tendency(IGDT)in the junior high... Internet gaming disorder(IGD)among junior high school students is an increasingly prominent mental health concern.It is important to look for influences behind internet gaming disorder tendency(IGDT)in the junior high school student population.The present study aimed to reveal the explanatory mechanisms underlying the association between parental psychological control(PPC)and internet gaming disorder tendency among junior high school students by testing the mediating role of core self-evaluation(CSE)and the moderating role of intentional self-regulation(ISR).Participants in present study were 735 Chinese junior high school students who completed offline self-report questionnaires on parental psychological control,core self-evaluation,intentional self-regulation,and Internet gaming disorder tendency.Analyses were conducted via mediation and moderated mediation.The results showed that:(1)Parental psychological control was positively related to junior high school students’Internet gaming disorder tendency.Core self-evaluation,and intentional self-regulation were negatively related to junior high school students’Internet gaming disorder tendency,respectively.(2)Core self-evaluation partially mediated the relationship between parental psychological control and junior high school students’Internet gaming disorder tendency.(3)Intentional self-regulation moderated the association between parental psychological control and Internet gaming disorder tendency,as well as the relationships between parental psychological control and core self-evaluation and core self-evaluation and Internet gaming disorder tendency in the mediated model.Based on these findings,we believe that there is a need to weaken parental psychological control,strengthen junior high school students’core self-evaluation and intentional self-regulation,and to recognize the important role of parents as well as their children’s personal positive traits in the healthy development of junior high school students. 展开更多
关键词 Internet gaming disorder tendency parental psychological control core self-evaluation intentional self-regulation moderated mediation model junior high school students
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Construction of Psychological Adjustment Function Model of Music Education Based on Emotional Tendency Analysis
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作者 Bin Zhang 《International Journal of Mental Health Promotion》 2023年第5期655-671,共17页
In the face offierce competition in the social environment,mental health problems gradually get the attention of the public,in order to achieve accurate mental health data analysis,the construction of music education ... In the face offierce competition in the social environment,mental health problems gradually get the attention of the public,in order to achieve accurate mental health data analysis,the construction of music education is based on emotional tendency analysis of psychological adjustment function model.Design emotional tendency analysis of music education psychological adjustment function architecture,music teaching goal as psychological adjust-ment function architecture building orientation,music teaching content as a foundation for psychological adjust-ment function architecture and music teaching process as a psychological adjustment function architecture building,music teaching evaluation as the key of building key regulating function architecture,Establish a core literacy oriented evaluation system.Different evaluation methods were used to obtain the evaluation results.Four levels of psychological adjustment function model of music education are designed,and the psychological adjust-ment function of music education is put forward,thus completing the construction of psychological adjustment function model of music education.The experimental results show that the absolute value of the data acquisition error of the designed model is minimum,which is not more than 0.2.It is less affected by a bad coefficient and has good performance.It can quickly converge to the best state in the actual prediction process and has a strong con-vergence ability. 展开更多
关键词 Emotional tendency analysis music education psychological adjustment functional model core literacy orientation
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Self-heating tendency evaluation of sulfide ores based on nonlinear multi-parameters fusion 被引量:1
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作者 潘伟 吴超 +1 位作者 李孜军 杨月平 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2015年第2期582-589,共8页
In order to reveal the nonlinear dynamics characteristics of unsteady self-heating process of sulfide ores, nine different kinds of sulfide ore samples from a pyrite mine in China were taken as experimental materials ... In order to reveal the nonlinear dynamics characteristics of unsteady self-heating process of sulfide ores, nine different kinds of sulfide ore samples from a pyrite mine in China were taken as experimental materials and their self-heating characteristics were measured in laboratory. Furthermore, the measured temperature was studied by integrating wavelet transform, nonlinear characteristic parameters extraction and fuzzy comprehensive evaluation. The results indicate that only the ore samples 1, 2, 6 and 9 have obvious self-heating phenomenon, and their self-heating initiative temperatures are 220 ℃, 239 ℃, 220 ℃ and 220 ℃, respectively, which means that they are difficult to produce self-heating under normal mining conditions. The correlation dimension of self-heating process is fraction and the maximum Lyapunov exponent is positive, which means that it is feasible to study the self-heating process based on chaotic dynamics theory. The nonlinearities of self-heating process of these four samples (ore samples 1, 2, 6 and 9) are 0.8227, 0.7521, 0.9401 and 0.8827 respectively and the order of the samples according to these results is: sample 6, sample 9, sample 1, sample 2, which is consistent with the measured results of self-heating characteristics. Therefore, the nonlinearity method can be used to evaluate the self-heating tendency of sulfide ores, and it is an effective verification of the reliability of measured results. 展开更多
关键词 sulfide ores self-heating process nonlinear characteristic parameter NONLINEARITY self-heating tendency
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薏苡仁油联合5-FU对结肠癌HCT-116细胞株增殖、凋亡的影响 被引量:1
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作者 王韬 费建东 +1 位作者 聂双发 李磊 《西部中医药》 2024年第1期9-12,共4页
目的:分析薏苡仁油联合5-氟尿嘧啶(5-fluorouracil,5-FU)对结肠癌HCT-116细胞株增殖、凋亡的影响及其作用机制。方法:采用四甲基偶氮唑盐(methyl thiazolyl tetrazdium,MTT)法测定不同浓度薏苡仁油、5-FU以及薏苡仁油+5-FU对人结肠癌HCT... 目的:分析薏苡仁油联合5-氟尿嘧啶(5-fluorouracil,5-FU)对结肠癌HCT-116细胞株增殖、凋亡的影响及其作用机制。方法:采用四甲基偶氮唑盐(methyl thiazolyl tetrazdium,MTT)法测定不同浓度薏苡仁油、5-FU以及薏苡仁油+5-FU对人结肠癌HCT-116细胞株的生长抑制效应;采用流式细胞术检测细胞凋亡情况;采用逆转录聚合酶链反应(RT-PCR)检测生存蛋白(Survivin)mRNA表达水平。结果:薏苡仁油、5-FU、5-FU+薏苡仁油均对人结肠癌HCT-116细胞增殖产生一定抑制作用,且薏苡仁油浓度越高,作用时间越长,细胞增殖抑制率越高,3组比较差异有统计学意义(P<0.05);与薏苡仁油组及5-FU组相比,5-FU+薏苡仁油组细胞增殖抑制率更高,差异有统计学意义(P<0.05)。薏苡仁油组、5-FU组、5-FU+薏苡仁油组的细胞凋亡率均高于空白对照组,差异有统计学意义(P<0.05);薏苡仁油浓度越高,细胞凋亡率也越高,差异有统计学意义(P<0.05);5-FU+薏苡仁油组细胞凋亡率高于5-FU组及薏苡仁油组,差异有统计学意义(P<0.05)。与空白对照组相比,不同浓度薏苡仁油组Survivin mRNA表达降低,差异有统计学意义(P<0.05);5-FU组Survivin mRNA表达升高,差异有统计学意义(P<0.05);5-FU+薏苡仁油组Survivin mRNA表达较5-FU组降低,差异有统计学意义(P<0.05)。结论:薏苡仁油能抑制结肠癌HCT-116细胞增殖,诱导细胞凋亡,与5-FU联合应用能发挥药物协同作用,其作用机制可能与调控Survivin mRNA表达有关。 展开更多
关键词 结肠癌 增殖 凋亡 薏苡仁油 5-氟尿嘧啶
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SLC6A9对结直肠癌细胞生长和对5-FU药物敏感性的影响 被引量:1
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作者 张岩 田素礼 +1 位作者 周勇旭 刘昶 《现代肿瘤医学》 CAS 2024年第7期1236-1241,共6页
目的:研究溶质载体家族6成员9(solute carrier family 6 member 9,SLC6A9)表达对结直肠癌细胞增殖、迁移和5-氟尿嘧啶(5-fluorouracil,5-FU)药物敏感性的影响。方法:TCGA数据库分析、实时荧光定量PCR和Western blot分析检测SLC6A9在结... 目的:研究溶质载体家族6成员9(solute carrier family 6 member 9,SLC6A9)表达对结直肠癌细胞增殖、迁移和5-氟尿嘧啶(5-fluorouracil,5-FU)药物敏感性的影响。方法:TCGA数据库分析、实时荧光定量PCR和Western blot分析检测SLC6A9在结肠癌组织、正常结肠细胞系(NCM460)和结直肠癌细胞系(SW620、HCT116、HT29、Lovo和SW480)中的表达。将SCL6A9过表达质粒及阴性对照(SLC6A9 OE、Vector)转染HT29细胞,将SCL6A9小干扰RNA及阴性对照(SLC6A9 siRNA1#、siRNA2#和Scramble)转染SW620细胞。划痕愈合实验和Transwell实验检测各组细胞的迁移、侵袭能力。Western blot和细胞免疫荧光检测EMT相关蛋白E-cadherin、Vimentin的表达水平。利用CCK-8法和构建裸鼠移植瘤模型检测SLC6A9过表达对结直肠癌细胞5-FU药物敏感性的影响。结果:与正常结肠组织和NCM460细胞相比,SLC6A9在结肠癌组织和结直肠癌细胞系中低表达(均P<0.05)。SLC6A9过表达引起E-cadherin蛋白表达增加,Vimentin蛋白水平降低,抑制结直肠癌细胞的迁移、侵袭(P<0.05)。SLC6A9低表达引起E-cadherin蛋白表达降低,Vimentin蛋白水平增加,促进结直肠癌细胞的迁移、侵袭能力(P<0.05)。SLC6A9过表达提高了5-FU的药物敏感性,并使肿瘤生长缓慢,质量减轻(P<0.05)。而SLC6A9低表达降低了5-FU的药物敏感性(P<0.05)。结论:SLC6A9过表达能够抑制结直肠癌细胞的迁移、侵袭和EMT进程,并增强5-FU对结直肠癌细胞的药物敏感性。 展开更多
关键词 结肠肿瘤 SLC6A9 细胞迁移和侵袭分析 EMT 5-fu 异种移植模型实验
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ONECUT2高表达介导胃癌复发患者5-FU化疗耐药
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作者 朱海峰 陈述 +1 位作者 龙卫国 王旭 《江苏大学学报(医学版)》 CAS 2024年第1期19-24,共6页
目的:探究One cut结构域家族成员2(ONECUT2)是否可以作为对5-FU耐药的胃癌复发患者的治疗靶点。方法:利用癌症基因组图谱(TCGA)数据库分析ONECUT2基因在胃癌患者及胃癌复发患者的表达情况。利用基因集富集分析(GSEA)评估TCGA数据库中ONE... 目的:探究One cut结构域家族成员2(ONECUT2)是否可以作为对5-FU耐药的胃癌复发患者的治疗靶点。方法:利用癌症基因组图谱(TCGA)数据库分析ONECUT2基因在胃癌患者及胃癌复发患者的表达情况。利用基因集富集分析(GSEA)评估TCGA数据库中ONECUT2表达量与药物代谢相关生物学过程的相关性。利用CCK-8细胞毒性实验评估胃癌细胞敲低或过表达ONECUT2对5-FU的耐药情况。分析30例胃癌术后复发患者ONECUT2表达量与5-FU治疗效果的相关性。结果:TCGA数据库分析显示,在经受过化疗且复发的胃癌患者中,ONECUT2高表达的患者无病间隔期(disease-free interval,DFI)更短。GSEA分析结果表明,ONECUT2高表达与异生药物代谢通路呈正相关,这提示可能与胃癌患者的耐药相关。体外实验中,敲低ONECUT2降低胃癌细胞对5-FU的半数抑制浓度(IC_(50))值;反之,过表达ONECUT2增加细胞对5-FU的IC_(50)值。30例胃癌复发患者对5-FU的药物敏感性与ONECUT2表达量相关。结论:胃癌复发患者的ONECUT2表达量更高,ONECUT2高表达与胃癌复发患者的5-FU耐药相关,可能是潜在的治疗靶点。 展开更多
关键词 One cut结构域家族成员2 胃癌复发 5-fu耐药 GSEA 细胞毒性 半数抑制浓度
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山萘酚逆转肝癌耐药细胞Bel-7402/5-Fu的作用机制研究
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作者 梁大敏 杨正久 +2 位作者 张子萍 钱静 毛朝坤 《天津医药》 CAS 2024年第9期900-906,共7页
目的探讨山萘酚(KAE)对肝癌耐药细胞Bel-7402/5-Fu功能的影响。方法采用KAE处理Bel-7402/5-Fu细胞,将细胞分为对照组和药物组(0.064、0.320、1.600、8、40、200μmol/L KAE);根据干扰DNA依赖性激酶催化亚基(DNA-PKcs)、加入蛋白酶体抑制... 目的探讨山萘酚(KAE)对肝癌耐药细胞Bel-7402/5-Fu功能的影响。方法采用KAE处理Bel-7402/5-Fu细胞,将细胞分为对照组和药物组(0.064、0.320、1.600、8、40、200μmol/L KAE);根据干扰DNA依赖性激酶催化亚基(DNA-PKcs)、加入蛋白酶体抑制剂MG132或加入自噬抑制剂CQ,将细胞分为si-NC组和DNA-PKcs干扰(siRNA-1664、siRNA-2142、siRNA-3785)组,对照组、KAE组和KAE+si-DNA-PKcs组,对照组、KAE+DMSO组、KAE+MG132组和KAE+CQ组。采用CCK-8检测细胞增殖,实时荧光定量PCR(RT-qPCR)和Western blot检测组蛋白H_(2)AX磷酸化(γ-H_(2)AX)、DNA-PKcs、DNA双链断裂修复/V(D)J重组蛋白(Artemis)和药泵基因(P-gp)mRNA和蛋白的表达,流式细胞术检测细胞周期和细胞凋亡,蛋白稳定性实验检测DNA-PKcs蛋白稳定性,免疫共沉淀检测DNA-PKcs蛋白泛素化。结果与对照组相比,8μmol/L KAE处理细胞24 h可抑制约50%的细胞增殖能力,选择此时间和浓度进行后续研究。与对照组相比,KAE组γ-H_(2)AX mRNA和蛋白表达水平升高,DNA-PKcs、Artemis和P-gp mRNA和蛋白表达水平降低(P<0.05);与对照组相比,KAE促进Bel-7402/5-Fu细胞周期阻滞于G2/M期,增加细胞凋亡;与si-NC组相比,siRNA-1664能显著下调DNA-PKcs mRNA和蛋白表达水平(P<0.05);与KAE组相比,KAE+si-DNAPKcs组进一步促进了KAE对细胞的效应;与对照组相比,KAE+DMSO组DNA-PKcs蛋白表达水平降低(P<0.05);与KAE+DMSO组相比,KAE+MG132组DNA-PKcs蛋白表达水平升高(P<0.05),而KAE+CQ组DNA-PKcs蛋白表达水平无明显变化(P>0.05);与对照组相比,KAE+DMSO组促进DNA-PKcs蛋白的泛素化,而KAE+MG132组则可抑制其泛素化(P<0.05)。结论KAE能够诱导肝癌耐药细胞Bel-7402/5-Fu的细胞凋亡和细胞周期阻滞。 展开更多
关键词 肝细胞 山萘酚 细胞凋亡 细胞周期 Bel-7402/5-fu DNA-PKCS
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Paleostress Reconstruction from 3D Seismic Data and Slip Tendency in the Northern Slope Area of the Bongor Basin, Southwestern Chad
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作者 Hakro Ahmed Suhail Xintao Yuan +3 位作者 Jianlin Li Hong Liu Yuan Liu Cong Ma 《Open Journal of Geology》 CAS 2023年第5期536-578,共43页
Paleostress plays a significant role in controlling the formation, accumulation, and distribution of reservoirs, and this could be an important factor in controlling the production of hydrocarbons from the unconventio... Paleostress plays a significant role in controlling the formation, accumulation, and distribution of reservoirs, and this could be an important factor in controlling the production of hydrocarbons from the unconventional reservoirs. In this study, we will use 3D seismic reflection data to perform the slip-tendency-based stress inversion to determine the stress field in the basement of the northern slope area in the Bongor Basin. The dataset for this technique is easily available in the oil and gas companies. The stress inversion results from the basement of the northern slope area of Bongor basin show that the maximum principal stress axis (σ1) is oriented vertically, the intermediate principal stress axis (σ2) is oriented N18° and the minimum principal stress axis (σ3) is oriented N105°, and σ2/σ1 = 0.60 and σ3/σ1 = 0.29. The findings of this paper provide significant information to understand the fault reactivation at the critical stage of hydrocarbon accumulation and the regional tectonic evolution. 展开更多
关键词 Seismic Reflection Data Slip tendency Bongor Basin Stress Inversion
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The Effect of Self-Investment on Hoarding Tendency of Chinese College Students:Role of Psychological Connections
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作者 Xiangli Guan Yue Zhang +6 位作者 Yang Li Yaqi Zhang Jingjing Wang Xuejiao Li Mary C.Jobe Md Zahir Ahmed Oli Ahmed 《International Journal of Mental Health Promotion》 2023年第6期755-766,共12页
Because of factors such as energy and time one invests in an object,the stronger the connection,value,and reluc-tance to lose said object individual will have.Hoarding behavior arises when individuals incorporate a st... Because of factors such as energy and time one invests in an object,the stronger the connection,value,and reluc-tance to lose said object individual will have.Hoarding behavior arises when individuals incorporate a strong attachment with themselves to an object.The purpose of this study is to examine the effect of self-investment on hoarding tendency and the roles of possession-self link and liking level in this connection.A hypothetical model of the relationship between self-investment,possession-self link,liking level,and hoarding tendency was tested.A convenience sampling method was used to survey 450 college students in Yunnan Province on either a paper-based or online self-report scale.The data were collected using self-investment,possession-self link,and liking level questionnaires,as well as the Saving Inventory Revised.Results showed positive relationship between the study variables,ranging from 0.37 to 0.87.College students’self-investment had a direct positive pre-dictive effect on hoarding tendency;self-investment,in turn,indirectly predicted hoarding tendency through the mediating effect of possession-self link;and individual liking level of items had a moderating effect for self-invest-ment on the possession-self link.This study shows how self-investment affects the hoarding tendency of college students,and the results of this study also help demonstrate the value of self-investment and possession-self link in optimizing students’hoarding tendency and thus promoting good psychological status. 展开更多
关键词 Hoarding tendency self-investment psychological connections possession-self link liking level
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盐浓度对FUS蛋白相分离的调控研究
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作者 陈家媛 王艳伟 《生物过程》 2024年第2期89-97,共9页
肉瘤融合蛋白(Fused in sarcoma, FUS)是来自异质核糖核蛋白家族的RNA结合蛋白。FUS在细胞内参与了DNA损伤修复,RNA剪接、转录、翻译等多种生理过程,这些过程与其生理条件下通过液液相分离(liquid-liquid phase separation, LLPS)形成... 肉瘤融合蛋白(Fused in sarcoma, FUS)是来自异质核糖核蛋白家族的RNA结合蛋白。FUS在细胞内参与了DNA损伤修复,RNA剪接、转录、翻译等多种生理过程,这些过程与其生理条件下通过液液相分离(liquid-liquid phase separation, LLPS)形成的类液滴状无膜细胞结构密切相关。在这个过程中FUS通过LLPS形成的生理性液滴状结构可能会老化成为病理性的聚集体,如水凝胶、包涵体和淀粉样纤维等。并且这些病理性聚集体被发现与肌萎缩性侧索硬化症(Amyotrophic lateral sclerosis, ALS)、额颞叶变性(Frontotemporal lobe degeneration, FTLD)等多种神经退行性疾病相关。本文进一步研究了影响FUS发生LLPS和病理性沉淀的相关因素,我们发现FUS蛋白LLPS和形成病理性聚集体是蛋白质浓度依赖的。在低蛋白浓度下FUS不会发生相分离,随着蛋白浓度的升高FUS蛋白开始发生LLPS现象,但是当蛋白浓度继续升高时则会形成淀粉样纤维聚集体。值得注意的是,这个过程受到盐浓度的显著影响,无盐和低盐浓度下发生LLPS的FUS蛋白,却在中、高盐浓度时迅速形成病理性的不规则聚集体。在FUS蛋白的LLPS过程中随着时间的变化液滴会不断变大,液滴在不同溶液中的生长效率为:无盐 > KCl > NaCl。 展开更多
关键词 fuS 液液相分离 病理性聚集体
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Role of glutathione transferase in phase separation of FUS and TAF15 in neurons
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作者 Kiyoung Kim 《Neural Regeneration Research》 SCIE CAS 2025年第7期1999-2000,共2页
Amyotrophic lateral sclerosis(ALS)is a progressive neurodegenerative disorder characterized by the degeneration of motor neurons in the brain and spinal cord,leading to muscle weakness,paralysis,and ultimately death(C... Amyotrophic lateral sclerosis(ALS)is a progressive neurodegenerative disorder characterized by the degeneration of motor neurons in the brain and spinal cord,leading to muscle weakness,paralysis,and ultimately death(Cleveland and Rothstein,2001).Frontotemporal lobar degeneration(FTLD)is a neurodegenerative disease affecting the frontal and temporal lobes of the brain,leading to changes in behavior,personality,and language(Van Langenhove et al.,2012).Both ALS and FTLD are classified as proteinopathies in which abnormal protein aggregation and accumulation in neurons contribute to the disease pathogenesis.Fused in sarcoma(FUS)is a DNA/RNA-binding protein involved in various cellular processes,including transcriptional regulation,RNA splicing,and DNA repair.Mutations in the FUS gene have been linked to familial ALS,highlighting the importance of FUS in the disease pathogenesis(Vance et al.,2009).In ALS and FTLD,aberrant post-translational modifications(PTMs)of FUS,such as phosphorylation,acetylation,and methylation,have been implicated in the promotion of FUS aggregation and neurotoxicity(Choi et al.,2023).Therefore,understanding the regulatory mechanisms of FUS PTMs is crucial for developing targeted therapies against these diseases. 展开更多
关键词 DEGENERATION fuS AGGREGATION
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一步/分步载药法对LDH/5-FU的粒径及形貌影响
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作者 李雪 王怡轩 张瑞平 《广州化工》 CAS 2024年第5期61-63,共3页
层状双氢氧化物(LDH)是一种带正电性的无机层状载体,其用于负载电负性的药物可实现药物的缓控释。使用了一步载药法及分步载药法将带负电的药物5-氟尿嘧啶(5-FU)负载于LDH层间,合成了药物递送系统LDH/5-FU。通过不同的载药方法得到了LDH... 层状双氢氧化物(LDH)是一种带正电性的无机层状载体,其用于负载电负性的药物可实现药物的缓控释。使用了一步载药法及分步载药法将带负电的药物5-氟尿嘧啶(5-FU)负载于LDH层间,合成了药物递送系统LDH/5-FU。通过不同的载药方法得到了LDH/5-FU,拍摄了其透射电镜图,并观察了其粒径分布。结果表明,一步载药法合成的LDH/5-FU粒径在2 000 nm左右,PDI较大,分布不均匀,易团聚;分步载药法合成的LDH/5-FU粒径在200 nm左右,PDI相对较小,分布更为均匀。 展开更多
关键词 层状双氢氧化物 5-氟尿嘧啶 药物负载 LDH/5-fu
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The Current Situationand Future Tendency of Corporate Governance Reform in China
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作者 Zengyao Zhao Weizhou Zhong 《Chinese Business Review》 2003年第1期40-45,共6页
The state-owned enterprises(SOEs) reform in China has plunged into the most urgent stage, and more than 20 years' market-oriented reform of SOEs in China seems to have no enough economic efficiency. This paper disc... The state-owned enterprises(SOEs) reform in China has plunged into the most urgent stage, and more than 20 years' market-oriented reform of SOEs in China seems to have no enough economic efficiency. This paper discusses some issues concerning corporate governance reform of SOEs in China. In section A, we first analyze the current situation and major issues in corporate governance reform of SOEs in China. In Section B, we discuss how to further reform SOEs' corporate governance in China. Final section discusses the possible model of China's corporate governance. 展开更多
关键词 current situation future tendency corporate governance reform
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Research and Review on the Java Multi-thread Programming and Its Further Development Tendency
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作者 Jiangtao Geng 《International Journal of Technology Management》 2016年第5期4-6,共3页
In this paper, we conduct research on the Java multi-thread programming and its further development tendency. Multithreading mechanisms can run several programs at the same time, make the program run effi ciency becom... In this paper, we conduct research on the Java multi-thread programming and its further development tendency. Multithreading mechanisms can run several programs at the same time, make the program run effi ciency becomes higher that also can overcome the problem of basic traditional programming language design while its design is the key to the realization of the synchronous thread. Multithreading is a mechanism that allows concurrent execution of multiple instruction stream in the program, each instruction stream is called a thread, independent from each other between each other. Thread is also known as a lightweight process, it have independent execution and process control. Our research starts from the analysis of the corresponding mechanism to enhance the performance that is innovative and meaningful. 展开更多
关键词 REVIEW MULTI-THREAD Development tendency PROGRAMMING COMPUTER SCIENCE
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SETD5介导AKT1磷酸化调控结肠癌细胞的迁移和5-FU敏感性
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作者 黄开禹 史建国 程勇 《中国肿瘤生物治疗杂志》 CAS CSCD 北大核心 2024年第6期586-591,共6页
目的:探讨含SET结构域蛋白5(SETD5)对结肠癌细胞增殖、迁移和对5-氟尿嘧啶(5-FU)药物敏感性的影响及机制。方法:常规培养结肠癌细胞,用Lipofectamine 2000将siSETD5-NC、si-SETD5-1~3质粒转染至HT-29细胞中,将其分为对照组(未处理)、si-... 目的:探讨含SET结构域蛋白5(SETD5)对结肠癌细胞增殖、迁移和对5-氟尿嘧啶(5-FU)药物敏感性的影响及机制。方法:常规培养结肠癌细胞,用Lipofectamine 2000将siSETD5-NC、si-SETD5-1~3质粒转染至HT-29细胞中,将其分为对照组(未处理)、si-SETD5-NC组、si-SETD5组和si-SETD5+SC79组,si-SETD5+SC79组HT-29细胞转染质粒的同时用10µmol/L SC79处理。qPCR法检测NCM460、HT-29和LoVo细胞中SETD5 mRNA表达,流式细胞术、细胞划痕法、WB法和CCK-8法分别检测各组HT-29细胞的凋亡情况、迁移能力、相关蛋白的表达,以及对5-FU的敏感性。结果:SETD5 mRNA在HT-29、LoVo细胞中均呈高表达(均P<0.01)。在HT-29细胞中成功地敲减了SETD5 mRNA(P<0.01)。敲减SETD5 mRNA可明显抑制HT-29细胞的增殖活性(P<0.01)、迁移能力(P<0.01)、相关蛋白(SETD5、p-PI3K、p-AKT1、p-mTOR蛋白)的表达(均P<0.01)、促进细胞凋亡(P<0.01),且提高其对5-FU的敏感性(P<0.01),这些作用均可被AKT激活剂SC79部分阻挡(P<0.05或P<0.01)。结论:SETD5在HT-29、LoVo细胞中高表达,SETD5通过PI3K/AKT1通路促进结肠癌HT-29细胞的增殖、迁移,且降低其对5-FU的敏感性,SETD5是结肠癌临床诊断、治疗的潜在靶点。 展开更多
关键词 结肠癌 含有SET结构域蛋白5 蛋白激酶B磷酸化 增殖 迁移 5-氟尿嘧啶 耐药
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儿茶素通过促进胃癌细胞铁死亡增强化疗药物5-FU的杀伤作用
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作者 徐涛 井文君 刘静 《现代肿瘤医学》 CAS 2024年第16期2981-2989,共9页
目的:探讨儿茶素对胃癌化疗效果的影响及机制。方法:使用5-FU(100μg/mL)处理胃癌细胞AGS和GC7901,分别处理0 h、3 h、6 h、9 h、12 h、16 h、24 h,通过PI染色及流式细胞术检测胃癌细胞AGS及GC7901的死亡情况;Western Blot实验检测正常... 目的:探讨儿茶素对胃癌化疗效果的影响及机制。方法:使用5-FU(100μg/mL)处理胃癌细胞AGS和GC7901,分别处理0 h、3 h、6 h、9 h、12 h、16 h、24 h,通过PI染色及流式细胞术检测胃癌细胞AGS及GC7901的死亡情况;Western Blot实验检测正常胃黏膜上皮细胞RGM-1、胃癌细胞AGS和GC7901铁死亡相关特征蛋白谷胱甘肽过氧化酶4(glutathione peroxidase 4,GPX4)、溶质载体家族7成员11(solute carrier family 7 member 11,SLC7A11)及重肽铁蛋白(ferritin heavy polypeptide,FTH)的表达,Image J软件测量灰度值;将胃癌细胞分别设置为对照组、5-FU处理组、5-FU联合Catechin处理组和5-FU联合DMSO处理组,分别通过PI染色及流式细胞术检测细胞死亡,通过C11-BODIPY581/591染色及流式细胞术检测脂质过氧化物积累,通过Western Blot实验检测铁死亡相关特征蛋白的表达,通过吸光光度法检测细胞裂解物中游离Fe^(2+)的含量;分离线粒体和细胞质组分,检测各个分组中线粒体内游离Fe^(2+)含量和细胞质内游离Fe^(2+)含量,并通过Mito-Ferro Green染色进一步验证增量Fe^(2+)的细胞内定位;Western Blot方法检测血红素加氧酶1蛋白(heme oxygenase 1,Hmox1)在各分组中的表达并通过Image J软件测量灰度值;将胃癌细胞分别设置为对照组、5-FU处理组、5-FU组联合Catechin处理组、5-FU联合Catechin和siRNA-NC处理组、5-FU联合Catechin和siRNA-Hmox1处理组,检测线粒体游离Fe^(2+)含量、脂质过氧化物积累及细胞死亡。结果:胃癌细胞在5-FU处理0~12 h内,随处理时间增长死亡率逐渐升高,然而在处理12~24 h内死亡率变化不明显表现出耐药性;胃癌细胞中铁死亡相关特征蛋白GPX4、SLC7A11和FTH的表达量显著高于正常胃黏膜上皮细胞(P<0.05);与对照组相比,处理24 h后,5-FU联合Catechin处理组细胞死亡数量、脂质过氧化物积累都显著升高(P<0.05),同时铁死亡相关特征蛋白GPX4、SLC7A11、FTH的表达量显著降低(P<0.05);5-FU联合Catechin处理组细胞质游离Fe^(2+)含量变化不明显而线粒体中游离Fe^(2+)含量显著升高(P<0.05),Hmox1蛋白表达量显著升高(P<0.05);与5-FU联合Catechin和siRNA-NC处理组相比,5-FU联合Catechin和siRNA-Hmox1处理组线粒体游离Fe^(2+)含量、脂质过氧化物积累和细胞死亡数量显著降低(P<0.05)。结论:儿茶素通过增强Hmox1的表达诱导线粒体铁离子浓度升高导致胃癌细胞脂质过氧化物积累促进5-FU诱导的胃癌细胞铁死亡。 展开更多
关键词 儿茶素 5-氟尿嘧啶 细胞铁死亡 二价铁离子 血红素加氧酶1
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LncRNA SOX2OT靶向SIRT1/自噬通路增强胆管癌细胞5-FU耐药
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作者 辛辰 王笑影 +5 位作者 李响 陈宇 王雪 宁佳曦 杨适 王忠琼 《南方医科大学学报》 CAS CSCD 北大核心 2024年第1期187-193,共7页
目的探讨LncRNASOX2OT靶向SIRT1/自噬通路调节胆管癌细胞5-FU耐药的机制。方法将未用5-FU处理HCCC-9810细胞和不同浓度(50、100、150、200μg/mL)5-FU处理的HCCC-9810细胞分为对照组和模型组,qRT-PCR检测LncRNA SOX2OT、SIRT1 mRNA表达... 目的探讨LncRNASOX2OT靶向SIRT1/自噬通路调节胆管癌细胞5-FU耐药的机制。方法将未用5-FU处理HCCC-9810细胞和不同浓度(50、100、150、200μg/mL)5-FU处理的HCCC-9810细胞分为对照组和模型组,qRT-PCR检测LncRNA SOX2OT、SIRT1 mRNA表达水平,Western blot检测SIRT1、Beclin1、LC3和p62蛋白表达。pcDNA3.1-SOX2OT和pcDNA3.1-NC转染HCCC-9810/5-FU耐药细胞,CCK-8检测细胞耐药性,划痕实验检测细胞迁移能力,qRT-PCR检测LncRNASOX2OT、SIRT1 mRNA表达水平,Western blot检测SIRT1、Beclin1、LC3和p62表达。在以上基础上做了Rescue实验,将OV-SOX2OT(2μg/孔)和si-NC(75pmol/孔)、OV-SOX2OT(2μg/孔)和si-SIRT1(75pmol/孔)分别共转染HCCC-9810/5-FU耐药细胞,分组为OV-SOX2OT+si-NC组和OV-SOX2OT+si-SIRT1组,以证明LncRNASOX2OT通过SIRT1影响自噬,并由此影响胆管癌细胞对5-FU的耐药性。RNAPulldown验证SOX2OT与SIRT1的靶向结合关系。结果不同浓度的5-FU均能抑制HCCC-9810细胞增殖(P<0.05)。与对照组相比,模型组SIRT1、Beclin1(P<0.001)和p62(P<0.01)蛋白表达、LC3Ⅱ/LC3Ⅰ比值(P<0.001)、SIRT1和LncRNA SOX2OT mRNA水平(P<0.05)均明显增加。与OV-NC组相比,OV-SOX2OT组细胞迁移能力、SIRT1、Beclin1(P<0.001)和p62(P<0.05)蛋白表达、LC3Ⅱ/LC3Ⅰ比值(P<0.001)、SIRT1和LncRNASOX2OTmRNA(P<0.05)水平均明显增加。沉默SIRT1表达导致LncRNASOX2OT过表达的HCCC-9810细胞对5-FU的耐药性显著降低。与OV-SOX2OT+si-NC组相比,OV-SOX2OT+si-SIRT1组SIRT1(P<0.001)、p62和Beclin1(P<0.01)蛋白表达、LC3Ⅱ/LC3Ⅰ比值(P<0.01)、SIRT1 mRNA(P<0.05)水平均明显降低。RNA Pulldown检测结果显示SOX2OT能够直接与SIRT1结合。结论LncRNA SOX2OT通过上调SIRT1表达促进自噬来增强胆管癌HCCC-9810细胞5-FU耐药性。 展开更多
关键词 LncRNASOX2OT SIRT1 自噬 胆管癌细胞 5-fu耐药
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敲减MET表达对喉癌Hep-2细胞增殖、迁移及5-FU和顺铂敏感性的影响
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作者 张翠红 肖淑芬 +3 位作者 张建军 贺占国 范才 马博敬 《中国肿瘤生物治疗杂志》 CAS CSCD 北大核心 2024年第9期857-863,共7页
目的:探究敲减间质表皮转化因子(MET)表达对人喉鳞状细胞癌(LSCC)Hep-2细胞生长、迁移及5-氟尿嘧啶(5-FU)和顺铂敏感性的影响。方法:通过基因表达综合数据库(GEO)及癌症基因组图谱(TCGA)数据库数据分析人LSCC组织中MET mRNA的表达水平;... 目的:探究敲减间质表皮转化因子(MET)表达对人喉鳞状细胞癌(LSCC)Hep-2细胞生长、迁移及5-氟尿嘧啶(5-FU)和顺铂敏感性的影响。方法:通过基因表达综合数据库(GEO)及癌症基因组图谱(TCGA)数据库数据分析人LSCC组织中MET mRNA的表达水平;常规培养正常人支气管上皮细胞16HBE与人LSCC细胞Hep-2、KBV200和TU212,采用qPCR法和WB法检测16HBE、Hep-2、KBV200和TU212细胞中MET基因和蛋白的表达水平。用LipofectamineTM3000将MET敲减质粒(si-Met)和对照质粒(si-NC)转染至Hep-2细胞中,分为空白对照组,si-NC组和si-Met组。采用MTT法、流式细胞术、划痕愈合实验分别检测各组Hep-2细胞的增殖、迁移能力、周期分布以及对5-FU和顺铂的敏感性。结果:数据库数据分析显示LSCC组织中MET mRNA呈高表达(P<0.05),Hep-2、KBV200和TU212细胞中MET mRNA和蛋白的表达水平也均明显高于16HBE细胞(均P<0.01)。敲减MET表达后,Hep-2细胞中METmRNA及蛋白表达水平均明显降低(P<0.01或P<0.001)、细胞增殖活力显著下降(P<0.0001)、G0/G1期细胞数量明显升高(P<0.0001)、S期细胞数量明显降低(P<0.0001)。敲减MET表达后,不同浓度5-FU或顺铂对细胞增殖的抑制率均显著升高、药物半数抑制浓度(IC50)均降低(均P<0.0001),划痕愈合率明显降低、迁移能力下降(均P<0.05)。结论:MET在人LSCC组织和细胞中呈高表达,敲减MET可有效抑制Hep-2细胞中MET的表达,抑制细胞增殖、迁移能力,使其周期阻滞于G1期,增强Hep-2细胞对5-FU和顺铂的敏感性。 展开更多
关键词 喉鳞状细胞癌 间质表皮转化因子 增殖 迁移 5氟尿嘧啶 顺铂 药物敏感性
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5-FU和角膜绷带镜在翼状胬肉切除联合自体角膜缘干细胞移植术中的应用 被引量:2
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作者 王越 叶丹 +3 位作者 申晓杰 马姝伟 李宁 单卫琢 《国际眼科杂志》 CAS 北大核心 2023年第12期2096-2099,共4页
目的:评价翼状胬肉切除联合自体角膜缘干细胞移植术(ALSCT)中联合应用5-氟尿嘧啶(5-FU)和角膜绷带镜治疗翼状胬肉的临床疗效。方法:随机对照临床试验。纳入2021-05/2022-11秦皇岛市海港医院眼科收治的翼状胬肉患者71例71眼:A组23眼行翼... 目的:评价翼状胬肉切除联合自体角膜缘干细胞移植术(ALSCT)中联合应用5-氟尿嘧啶(5-FU)和角膜绷带镜治疗翼状胬肉的临床疗效。方法:随机对照临床试验。纳入2021-05/2022-11秦皇岛市海港医院眼科收治的翼状胬肉患者71例71眼:A组23眼行翼状胬肉切除联合ALSCT,B组24眼于术中局部浸润和术后结膜下注射5-FU,C组24眼联合应用5-FU的同时并于术后配戴角膜绷带镜。比较三组患者术后1、3、7、14d的舒适度评分,术后1、3、7、14d,1mo的角膜愈合情况,术后3~6mo的手术疗效和并发症发生率。结果:术后1、3、7d舒适度评分和术后1、3d角膜愈合情况比较,C组患者优于A、B两组。三组间术后14d的舒适度评分和术后14d、1mo的角膜愈合情况比较无差异。随访3~6mo,A组有3眼复发,B组1眼复发,C组无复发。三组患者术后并发症发生率比较无差异。结论:联合应用5-FU和术后配戴角膜绷带镜可以优化翼状胬肉切除联合ALSCT术后眼部体验,加快角膜上皮修复,并提高的手术成功率。 展开更多
关键词 翼状胬肉 5-氟尿嘧啶(5-fu) 角膜绷带镜
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The Feminist Tendency in Hotel du Lac
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作者 朱晓静 《海外英语》 2019年第24期225-226,共2页
It is true that some important feminist literary theories apply to Hotel du Lac,which illustrates clearly Brookner’s deep concern over modern women’s predicament in favor of their interests,and thus proves her stron... It is true that some important feminist literary theories apply to Hotel du Lac,which illustrates clearly Brookner’s deep concern over modern women’s predicament in favor of their interests,and thus proves her strong tendency to feminism.And then it is pointed out that the reason for Brookner’s perverse attitude toward feminism mainly lies in her unwillingness to confine herself to the category of feminism and actually Brookner has transcended the more stereotypical feminism. 展开更多
关键词 FEMINIST tendency TRANSCENDENCE
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