A new organization and management mechanism with the local government as the main body was created during the post-disaster reconstruction of the "4. 20 " Lushan earthquake,in which the experiences and lesso...A new organization and management mechanism with the local government as the main body was created during the post-disaster reconstruction of the "4. 20 " Lushan earthquake,in which the experiences and lessons were drawn from the "5. 12"Wenchuan earthquake. Correspondingly, the local government conducted an exploration of the organization and management mode in the field of planning and construction. The effective experiences have been accumulated, including working out a full coverage planning,taking the leading part in the urban-rural planning,and exercising whole space control over the disaster area. An innovation management mode named as "Five Generals Pattern"was implemented in the construction projects. In view that the rural housing reconstruction was a weak link, a new approach to improving the construction management was investigated,by means of strengthening on-site supervision,establishing a technical platform,and bolstering public participation.展开更多
This paper is the analysis of the main body, the object and their relationship concerning research of traditional Chinese medicine. The result proved that the main body of research of traditional Chinese medicine is r...This paper is the analysis of the main body, the object and their relationship concerning research of traditional Chinese medicine. The result proved that the main body of research of traditional Chinese medicine is researchers who possessed the fundamentals of traditional Chinese medicine. It is the objects of research of traditional Chinese medicine that origin of a class of traditional Chinese medical literature consisting of herbals which focus on descriptions of individual drugs; the relationship of the technological process of preparing Chinese medicine, as by parching, roasting, baking, steaming, soaking, and simmering, etc.;and technology of separating, extracting and purifying for composition; traditional Chinese medicine dynamics of one of the 12 two-hour periods into which the day was formerly divided before the introduction of western chronology; compatibility of medicines in active compositions; patent, effectiveness for a given period of time and constantly changing of traditional Chinese medicine.The relationship between the main body and the objects was that the former depends on the latter ones and the main body has subjective initiative as fully as possible.展开更多
Relying on the relevant data from China Statistical Yearbook and Chinese Rural Statistical Yearbook,the coolabarative performance of main body of agricutlural technology innovation is evaluated by taking quaternary-pa...Relying on the relevant data from China Statistical Yearbook and Chinese Rural Statistical Yearbook,the coolabarative performance of main body of agricutlural technology innovation is evaluated by taking quaternary-parties innovation body as the sujects and by establishing the index system from the perspectives of input and output.The results show serious dislocation of main body and bad collabrative performance.On the basis,the quaternary-parties motivation system cocerning agricutlural enterpises,agricutlural science and technology acanamy,rural hosueholds and governments is established.The system is capable of integrating market,sciencetific reserch and diffusion collection into a whole part and solving the seperation of agricutlural innovation supply and demand.展开更多
The current agricultural conflicts of China are analyzed and the forms and drawbacks of current agricultural industrial structure are listed.The situations for intensifying the farmland with appropriate scale are anal...The current agricultural conflicts of China are analyzed and the forms and drawbacks of current agricultural industrial structure are listed.The situations for intensifying the farmland with appropriate scale are analyzed from the aspects of policy,farmers,farms and modernization of agriculture.It is pointed out that the situations for the intensive use of land are becoming mature.Taking the single pig-breeding chain as an example,the agricultural industrialization model,which takes farmers as the main body,is expounded.Besides,its functions and significance in solving "the three agriculture problems" and facilitating the modernization of agriculture are discussed.展开更多
The nationally registered rice varieties during 2006-2015 were analyzed and there were 49.4 varieties on average annually registered in the last decade. In terms of the structure of varieties, there was a decreasing t...The nationally registered rice varieties during 2006-2015 were analyzed and there were 49.4 varieties on average annually registered in the last decade. In terms of the structure of varieties, there was a decreasing trend for the proportion of three-line hybrid rice varieties but a rapid increase for that of two-line hybrid rice varieties. The percentage of rice varieties developed by scientific research institu- tions was decreasing but that developed by seed companies kept a rapid increase, indicating the commercial breeding conducted by seed companies has become a principal model in rice breeding. With regard to grain quality of varieties, the con- ventional rice varieties showed an increasing trend while the hybrid rice varieties had a decreasing trend in the proportion of high quality varieties, indicating hybrid rice needs improving more in grain quality in breeding programs.展开更多
In this paper, progress in strain study of blocks and faults by GPS data are discussed, and the concept that active structures between blocks are the main body of crustal strain is clarified. By energy transfer princi...In this paper, progress in strain study of blocks and faults by GPS data are discussed, and the concept that active structures between blocks are the main body of crustal strain is clarified. By energy transfer principle of elastic mechanics, the relation between strain around faults and tectonic force on fault surfaces is set up and main body element model of crustal strain is constructed. Finally, the relation between mechanical evolution of model and seismogenic process of Kunlun earthquake (Ms=8.1) is discussed by continuous GPS data of datum stations. The result suggests that the relatively relaxed change under background of strong compressing and shearing may help to trigger moderate-strong earthquakes.展开更多
This paper expounds the connotation of main body of new socialist village construction,and the duplex main body characteristics of farmers and government.It points out that the main body of society should be the state...This paper expounds the connotation of main body of new socialist village construction,and the duplex main body characteristics of farmers and government.It points out that the main body of society should be the state holding public power,and the main body of nature or practice is the farmer who participates in production and living activities in rural areas and supports the existence of rural society.It also expounds the dominant position of duplex main body in new village construction,wherein the government is the main body which administers public power in the field of public power in new village construction,while the farmer is the main body who administers private rights in the field of non-public rights in new village construction.It generalizes and unravels the role of duplex main body of farmers and government in new village construction,and analyzes the relationship of role of duplex main body between government and farmers as follows:the government dominates over rural development,and the government,as main body,plays dominant role in the field of rural public power and construction of social environment,while the farmers are main participators and constructors,who play significant role;in construction of rural non-public power field,the farmers play the role of main body,and the government plays the role of guidance and service.In new village construction,the government and farmers play the role abreast,which is an important prerequisite and guarantee of constructing new village.In the process of government's fulfilling responsibility in new village construction,the outdated administrative concept exists,and in the process of administering and promoting new village construction,the farmers' quality is yet to be promoted.As for these problems,three countermeasures are put forward as follows:transform concept;transform style of work and transform and exert advantage of organization.展开更多
The foundation of the ultrasonic non-destructive determination of stresses in near-the-surface layers of solids is presented. The method is based on the regularities of Rayleigh wave propagation in solids with initial...The foundation of the ultrasonic non-destructive determination of stresses in near-the-surface layers of solids is presented. The method is based on the regularities of Rayleigh wave propagation in solids with initial (residual) stresses. A description of the above mentioned method and examples of stress determination are presented. Examples are considered as applied to the residual stresses arising at electric welding and the operating stresses arising at loading.展开更多
AFLP fingerprinting of the 98 main sweetpotato varieties planted in China has been constructed. Using 17 AFLP primer combinations which were selected from 1 208 primer combinations and generated the most amounts of po...AFLP fingerprinting of the 98 main sweetpotato varieties planted in China has been constructed. Using 17 AFLP primer combinations which were selected from 1 208 primer combinations and generated the most amounts of polymorphic bands, AFLP analysis of the 98 main sweetpotato varieties gave a total of 410 clear polymorphic bands with an average of 24.12 polymorphic bands per primer combination. Each one of the 98 sweetpotato varieties could be clearly distinguished by EcoR I-cta/Mse I-ggc primer combination which generated the most polymorphic bands. AFLP-based genetic distance ranged from 0.0546 to 0.5709 with an average of 0.3799. The dendrogram based on AFLP markers indicated that sweetpotato varieties coming from the same regions or having same parents were clustered in the same groups. Analysis of molecular variance (AMOVA) revealed greater variations within regions (94.08%) than among regions (5.92%). Thus, the genetic variations mainly existed within regions, while the variations among regions were very low in the tested sweetpotato varieties. Significant genetic variations existed between "Northern" and "Southern" sweetpotato varieties when Yangtze River was used as the dividing line.展开更多
The objective of this study was to estimate genetic parameters of body width(BW)to body length(BL)ratio(BW/BL)and of body weight traits(BWT)in turbot,and to elucidate the genetic mechanism of the two traits during ont...The objective of this study was to estimate genetic parameters of body width(BW)to body length(BL)ratio(BW/BL)and of body weight traits(BWT)in turbot,and to elucidate the genetic mechanism of the two traits during ontogeny by dynamic genetic analysis.From 3 to 27 months,BW,BL and BWT of each communally stocked fish were measured every 3 months.The BW/BL ratio was measured at different sampling ages.A twotrait animal model was used for genetic evaluation of traits.The results showed that the heritability values of BW/BL ratio ranged from 0.2168 to 0.3148,corresponding to moderate heritability.The BWT heritability values ranged from 0.2702 to 0.3479 corresponding to moderate heritability.The heritability of BW/BL ratio was lower than that of BWT,except at 3 months of age.Genetic correlation between BW/BL ratio and BWT decreased throughout the measurement period.Genetic correlations were higher than the phenotypic correlations.The current results for estimating genetic parameters demonstrate that the BW/BL ratio could be used as a phenotypic marker of fast-growing turbot,and the BW/BL ratio and BWT could be improved simultaneously through selective breeding.展开更多
According to the quarantine procedures for sugarcane introduction, thirty-three sugarcane varieties/materials from the United States, Bangladesh, Thailand and some other countries were quarantined. The result showed t...According to the quarantine procedures for sugarcane introduction, thirty-three sugarcane varieties/materials from the United States, Bangladesh, Thailand and some other countries were quarantined. The result showed that main diseases and pests that are concerned in quarantine were undiscovered. In addition, molecular detection results of Fiji disease and leaf scald disease were negative ; detection results of mosaic in six varleties/materials were positive; detection results of yellow leaf syndrome in eight varieties/materials were positive.展开更多
In order to train a student to be a person with ability,who can self-study,use knowledge flexibly and bring firth new ideas constantly,the constant development of society demands a full exertion of student-center appr...In order to train a student to be a person with ability,who can self-study,use knowledge flexibly and bring firth new ideas constantly,the constant development of society demands a full exertion of student-center approach in the process of education.Therefore,how to exert the student-center approach is a question that every teacher is facing.I think that we can exert the student-center approach from the following aspects.One,a teacher must change his teaching conception.He should know that it's the students who are the main body of teaching and the teacher can act as a guider.The other,by all means,we ought to make the students know the purpose of their study and the function of themselves in learning.We also ought to culture their interest in learning,inspire and strengthen their main body consciousness.the teacher should,on the basis of full understanding his students and textbook,exert the leading function of himself.In the course of teaching,the teacher can exert the advantage of advanced teaching method,such as situational language teaching,and advanced teaching instrument,such as multimedia,which will inspire the students' interest and conscious activities.We can train the students' imagining ability through encouragement 、guiding and extracurricular activities.We can improve the students' ability of self-study.Thus,the students' main body function will reach a better exertion and the effect of teaching will be improved.This will train the students to suit the need of society.展开更多
[Objectives]This study was conducted to identify the pathogen species and dominant species of sugarcane pokkah boeng in main varieties in Yunnan sugarcane areas,so as to promote the healthy and sustainable development...[Objectives]This study was conducted to identify the pathogen species and dominant species of sugarcane pokkah boeng in main varieties in Yunnan sugarcane areas,so as to promote the healthy and sustainable development of sugarcane industry.[Methods]Specific primers Fv-F3/Fv-R3 and Fp-F4/Fp-R4 were designed based on the ribosomal DNA non-internal transcribed spacer(rDNA-ITS)gene sequences of Fusarium verticillioides and Fusarium proliferarum,the main pathogens of sugarcane pokkah boeng,and 117 typical sugarcane pokkah boeng samples collected from main varieties in different sugarcane areas of Yunnan Province were detected and analyzed by PCR.[Results]Among the 117 sugarcane pokkah boeng samples,112 samples were detected with F.verticillioides with a positive detection rate of 95.7%;103 samples were detected with F.proliferarum with a positive detection rate of 88%;103 samples were infected by F.verticillioides+F.proliferarum,and the compound infection rate was 88%;and the two pathogens were not detected in 5 samples,which might be sugarcane pokkah boeng caused by other species.PCR amplification products of 23 F.verticillioides positive samples and 19 F.proliferarum positive samples from different sugarcane varieties in different sugarcane areas were sequenced.The BLAST alignment results showed that the sequences of the 23 amplification products of F.verticillioides shared 99.45%-100.00%homology with F.verticillioides(GenBank accession number:KU508286),and the sequences of the 19 amplification products of F.proliferarum shared 99.26%-100.00%homology with the sequence of F.proliferarum(GenBank accession number:MK252904).Part of the F.verticillioids and 11 F.proliferarum sequences were selected to construct a phylogenetic tree,and the phylogenetic analysis showed that they belonged to the F.verticillioids group and F.proliferarum group,respectively.The results showed that F.verticillioides and F.proliferarum were the main pathogens causing sugarcane pokkah boeng of the main sugarcane varieties in Yunnan,and there was a common phenomenon of compound infection.F.verticillioides was the dominant species in Pu er,Lincang,Honghe and Yuxi sugarcane areas,but the detection rate of F.proliferarum was also high,and there were other species.In the future,the discovery of resistant germplasm resources and breeding of resistant varieties should be carried out aiming at these two pathogens of sugarcane pokkah boeng.[Conclusions]The study provides technical support for rapid identification of sugarcane pokkah boeng pathogens,and scientific basis for breeding resistant varieties and scientific disease prevention and control.展开更多
基金sponsored by the Science and Technology Support Project Funds of Sichuan Province ( Item No. 2013FZ0009 )Educational Reform Project Funds of Sichuan AgriculturalUniversity ( Item No. 2015064 )the National Natural Science Foundation of China ( Item No. 51278421)
文摘A new organization and management mechanism with the local government as the main body was created during the post-disaster reconstruction of the "4. 20 " Lushan earthquake,in which the experiences and lessons were drawn from the "5. 12"Wenchuan earthquake. Correspondingly, the local government conducted an exploration of the organization and management mode in the field of planning and construction. The effective experiences have been accumulated, including working out a full coverage planning,taking the leading part in the urban-rural planning,and exercising whole space control over the disaster area. An innovation management mode named as "Five Generals Pattern"was implemented in the construction projects. In view that the rural housing reconstruction was a weak link, a new approach to improving the construction management was investigated,by means of strengthening on-site supervision,establishing a technical platform,and bolstering public participation.
文摘This paper is the analysis of the main body, the object and their relationship concerning research of traditional Chinese medicine. The result proved that the main body of research of traditional Chinese medicine is researchers who possessed the fundamentals of traditional Chinese medicine. It is the objects of research of traditional Chinese medicine that origin of a class of traditional Chinese medical literature consisting of herbals which focus on descriptions of individual drugs; the relationship of the technological process of preparing Chinese medicine, as by parching, roasting, baking, steaming, soaking, and simmering, etc.;and technology of separating, extracting and purifying for composition; traditional Chinese medicine dynamics of one of the 12 two-hour periods into which the day was formerly divided before the introduction of western chronology; compatibility of medicines in active compositions; patent, effectiveness for a given period of time and constantly changing of traditional Chinese medicine.The relationship between the main body and the objects was that the former depends on the latter ones and the main body has subjective initiative as fully as possible.
基金Supported by Qiqihar Key Projects of Social Philosophy "Research on Agricultural Technology Innovation system of Qiqihar City " (QSX2009B-04)
文摘Relying on the relevant data from China Statistical Yearbook and Chinese Rural Statistical Yearbook,the coolabarative performance of main body of agricutlural technology innovation is evaluated by taking quaternary-parties innovation body as the sujects and by establishing the index system from the perspectives of input and output.The results show serious dislocation of main body and bad collabrative performance.On the basis,the quaternary-parties motivation system cocerning agricutlural enterpises,agricutlural science and technology acanamy,rural hosueholds and governments is established.The system is capable of integrating market,sciencetific reserch and diffusion collection into a whole part and solving the seperation of agricutlural innovation supply and demand.
文摘The current agricultural conflicts of China are analyzed and the forms and drawbacks of current agricultural industrial structure are listed.The situations for intensifying the farmland with appropriate scale are analyzed from the aspects of policy,farmers,farms and modernization of agriculture.It is pointed out that the situations for the intensive use of land are becoming mature.Taking the single pig-breeding chain as an example,the agricultural industrialization model,which takes farmers as the main body,is expounded.Besides,its functions and significance in solving "the three agriculture problems" and facilitating the modernization of agriculture are discussed.
基金Supported by Social Undertaking and People's Livelihood Guarantee Science and Technology Innovation Fund(cstc2016shms-ztzx0013cstc2016shms-ztzx0032 and cstc2016shms-ztzx0017)~~
文摘The nationally registered rice varieties during 2006-2015 were analyzed and there were 49.4 varieties on average annually registered in the last decade. In terms of the structure of varieties, there was a decreasing trend for the proportion of three-line hybrid rice varieties but a rapid increase for that of two-line hybrid rice varieties. The percentage of rice varieties developed by scientific research institu- tions was decreasing but that developed by seed companies kept a rapid increase, indicating the commercial breeding conducted by seed companies has become a principal model in rice breeding. With regard to grain quality of varieties, the con- ventional rice varieties showed an increasing trend while the hybrid rice varieties had a decreasing trend in the proportion of high quality varieties, indicating hybrid rice needs improving more in grain quality in breeding programs.
基金National Natural Science Foundation of China (40274023) and National Program for Key Science & Technology Projects (2004BA601B01-02-01).
文摘In this paper, progress in strain study of blocks and faults by GPS data are discussed, and the concept that active structures between blocks are the main body of crustal strain is clarified. By energy transfer principle of elastic mechanics, the relation between strain around faults and tectonic force on fault surfaces is set up and main body element model of crustal strain is constructed. Finally, the relation between mechanical evolution of model and seismogenic process of Kunlun earthquake (Ms=8.1) is discussed by continuous GPS data of datum stations. The result suggests that the relatively relaxed change under background of strong compressing and shearing may help to trigger moderate-strong earthquakes.
基金Supported by The Provincial Education Department of Hubei
文摘This paper expounds the connotation of main body of new socialist village construction,and the duplex main body characteristics of farmers and government.It points out that the main body of society should be the state holding public power,and the main body of nature or practice is the farmer who participates in production and living activities in rural areas and supports the existence of rural society.It also expounds the dominant position of duplex main body in new village construction,wherein the government is the main body which administers public power in the field of public power in new village construction,while the farmer is the main body who administers private rights in the field of non-public rights in new village construction.It generalizes and unravels the role of duplex main body of farmers and government in new village construction,and analyzes the relationship of role of duplex main body between government and farmers as follows:the government dominates over rural development,and the government,as main body,plays dominant role in the field of rural public power and construction of social environment,while the farmers are main participators and constructors,who play significant role;in construction of rural non-public power field,the farmers play the role of main body,and the government plays the role of guidance and service.In new village construction,the government and farmers play the role abreast,which is an important prerequisite and guarantee of constructing new village.In the process of government's fulfilling responsibility in new village construction,the outdated administrative concept exists,and in the process of administering and promoting new village construction,the farmers' quality is yet to be promoted.As for these problems,three countermeasures are put forward as follows:transform concept;transform style of work and transform and exert advantage of organization.
文摘The foundation of the ultrasonic non-destructive determination of stresses in near-the-surface layers of solids is presented. The method is based on the regularities of Rayleigh wave propagation in solids with initial (residual) stresses. A description of the above mentioned method and examples of stress determination are presented. Examples are considered as applied to the residual stresses arising at electric welding and the operating stresses arising at loading.
基金China Agriculture Research System (Sweetpotato) and Chinese Universities Scientific Fund (2012YJ008)
文摘AFLP fingerprinting of the 98 main sweetpotato varieties planted in China has been constructed. Using 17 AFLP primer combinations which were selected from 1 208 primer combinations and generated the most amounts of polymorphic bands, AFLP analysis of the 98 main sweetpotato varieties gave a total of 410 clear polymorphic bands with an average of 24.12 polymorphic bands per primer combination. Each one of the 98 sweetpotato varieties could be clearly distinguished by EcoR I-cta/Mse I-ggc primer combination which generated the most polymorphic bands. AFLP-based genetic distance ranged from 0.0546 to 0.5709 with an average of 0.3799. The dendrogram based on AFLP markers indicated that sweetpotato varieties coming from the same regions or having same parents were clustered in the same groups. Analysis of molecular variance (AMOVA) revealed greater variations within regions (94.08%) than among regions (5.92%). Thus, the genetic variations mainly existed within regions, while the variations among regions were very low in the tested sweetpotato varieties. Significant genetic variations existed between "Northern" and "Southern" sweetpotato varieties when Yangtze River was used as the dividing line.
基金The Earmarked Fund for Modern Agro-Industry Technology Research System under contract No.CARS-47-G01the Ao Shan Talents Cultivation Program supported by Qingdao National Laboratory for Marine Science and Technology under contract No.2017ASTCP-OS04+2 种基金the Agricultural Fine Breed Project of Shandong under contract No.2016LZGC031the Chinese Academy of Fishery Sciences Basal Research Fund under contract No.2016HY-JC0302the National Key Research and Development Program under contract No.2018YFD0900102
文摘The objective of this study was to estimate genetic parameters of body width(BW)to body length(BL)ratio(BW/BL)and of body weight traits(BWT)in turbot,and to elucidate the genetic mechanism of the two traits during ontogeny by dynamic genetic analysis.From 3 to 27 months,BW,BL and BWT of each communally stocked fish were measured every 3 months.The BW/BL ratio was measured at different sampling ages.A twotrait animal model was used for genetic evaluation of traits.The results showed that the heritability values of BW/BL ratio ranged from 0.2168 to 0.3148,corresponding to moderate heritability.The BWT heritability values ranged from 0.2702 to 0.3479 corresponding to moderate heritability.The heritability of BW/BL ratio was lower than that of BWT,except at 3 months of age.Genetic correlation between BW/BL ratio and BWT decreased throughout the measurement period.Genetic correlations were higher than the phenotypic correlations.The current results for estimating genetic parameters demonstrate that the BW/BL ratio could be used as a phenotypic marker of fast-growing turbot,and the BW/BL ratio and BWT could be improved simultaneously through selective breeding.
基金Supported by Special Fund for Modern Agro-industry Technology Research System(NYCYTXGXCXTD-2)Scientific Research and Technological Development Project of Guangxi Guangxi Zhuang Autonomous Region(GKN1347012-1+2 种基金GKN 1347004-2)Natural Science Foundation of Guangxi Zhuang Autonomous Region(11166-02)Internaltional Scientific Exchange Program(2013DFA31600)
文摘According to the quarantine procedures for sugarcane introduction, thirty-three sugarcane varieties/materials from the United States, Bangladesh, Thailand and some other countries were quarantined. The result showed that main diseases and pests that are concerned in quarantine were undiscovered. In addition, molecular detection results of Fiji disease and leaf scald disease were negative ; detection results of mosaic in six varleties/materials were positive; detection results of yellow leaf syndrome in eight varieties/materials were positive.
文摘In order to train a student to be a person with ability,who can self-study,use knowledge flexibly and bring firth new ideas constantly,the constant development of society demands a full exertion of student-center approach in the process of education.Therefore,how to exert the student-center approach is a question that every teacher is facing.I think that we can exert the student-center approach from the following aspects.One,a teacher must change his teaching conception.He should know that it's the students who are the main body of teaching and the teacher can act as a guider.The other,by all means,we ought to make the students know the purpose of their study and the function of themselves in learning.We also ought to culture their interest in learning,inspire and strengthen their main body consciousness.the teacher should,on the basis of full understanding his students and textbook,exert the leading function of himself.In the course of teaching,the teacher can exert the advantage of advanced teaching method,such as situational language teaching,and advanced teaching instrument,such as multimedia,which will inspire the students' interest and conscious activities.We can train the students' imagining ability through encouragement 、guiding and extracurricular activities.We can improve the students' ability of self-study.Thus,the students' main body function will reach a better exertion and the effect of teaching will be improved.This will train the students to suit the need of society.
基金Supported by China Agriculture Research System of MOF and MARA(CARS-170303)Yunling Industry and Technology Leading Talent Training Program(2018LJRC56)Special Fund for the Construction of Modern Agricultural Industry Technology System in Yunnan Province.
文摘[Objectives]This study was conducted to identify the pathogen species and dominant species of sugarcane pokkah boeng in main varieties in Yunnan sugarcane areas,so as to promote the healthy and sustainable development of sugarcane industry.[Methods]Specific primers Fv-F3/Fv-R3 and Fp-F4/Fp-R4 were designed based on the ribosomal DNA non-internal transcribed spacer(rDNA-ITS)gene sequences of Fusarium verticillioides and Fusarium proliferarum,the main pathogens of sugarcane pokkah boeng,and 117 typical sugarcane pokkah boeng samples collected from main varieties in different sugarcane areas of Yunnan Province were detected and analyzed by PCR.[Results]Among the 117 sugarcane pokkah boeng samples,112 samples were detected with F.verticillioides with a positive detection rate of 95.7%;103 samples were detected with F.proliferarum with a positive detection rate of 88%;103 samples were infected by F.verticillioides+F.proliferarum,and the compound infection rate was 88%;and the two pathogens were not detected in 5 samples,which might be sugarcane pokkah boeng caused by other species.PCR amplification products of 23 F.verticillioides positive samples and 19 F.proliferarum positive samples from different sugarcane varieties in different sugarcane areas were sequenced.The BLAST alignment results showed that the sequences of the 23 amplification products of F.verticillioides shared 99.45%-100.00%homology with F.verticillioides(GenBank accession number:KU508286),and the sequences of the 19 amplification products of F.proliferarum shared 99.26%-100.00%homology with the sequence of F.proliferarum(GenBank accession number:MK252904).Part of the F.verticillioids and 11 F.proliferarum sequences were selected to construct a phylogenetic tree,and the phylogenetic analysis showed that they belonged to the F.verticillioids group and F.proliferarum group,respectively.The results showed that F.verticillioides and F.proliferarum were the main pathogens causing sugarcane pokkah boeng of the main sugarcane varieties in Yunnan,and there was a common phenomenon of compound infection.F.verticillioides was the dominant species in Pu er,Lincang,Honghe and Yuxi sugarcane areas,but the detection rate of F.proliferarum was also high,and there were other species.In the future,the discovery of resistant germplasm resources and breeding of resistant varieties should be carried out aiming at these two pathogens of sugarcane pokkah boeng.[Conclusions]The study provides technical support for rapid identification of sugarcane pokkah boeng pathogens,and scientific basis for breeding resistant varieties and scientific disease prevention and control.