Aim: Diabetic neuropathic pain is one of the pains which hardly respond to pharmaceutical treat. Today, various chemical and herbal compounds have been used to reduce pain. The aim of this study is to compare the effe...Aim: Diabetic neuropathic pain is one of the pains which hardly respond to pharmaceutical treat. Today, various chemical and herbal compounds have been used to reduce pain. The aim of this study is to compare the effect of date extract and melatonin in preventing pain in diabetic rats.Method: To study hyperalgesia response and to compare the effect of date extract and melatonin in preventing pain, hot plate and tail flick tests were used. After prescribing single dose of streptozotocin to rats and approving their diabetes, treatment rats received date extract (4ml/kg/day) or melatonin [10 mg/kg/day, intraperitoneally (i.p.)] for a period of 6 weeks. At the end of the sixth week, control and treated rats were examined by thermal pain response and explorative activity tests.Results: According to hot plate results, response time to thermal pain in treated group showed a significant decrease in comparison with the control group (P 0.01). Prescription of date extract increased response time to thermal pain in comparison with treated group (P 0.01), so that response time approximated to control group. Although melatonin approximated to the response time to control group, the significant difference was not observed among melatonin receivers and other groups. In the assessment of diabetic neuropathy on the explorative activity of rats in an open field behavioral test, total distance moved and rearing frequency were significantly decreased, while administration of date extract did also improve motor deficits induced by STZ. Conclusions:Findings of this study showed that date extract decreased thermal hyperalgesia and can prevent pain resulted from diabetic neuropathy.展开更多
Puerarin is a major active ingredient of the traditional Chinese plant medicine,Radix Puerariae,and commonly used in the treatment of myocardial and cerebral ischemia.However,the effects of puerarin on neuropathic pai...Puerarin is a major active ingredient of the traditional Chinese plant medicine,Radix Puerariae,and commonly used in the treatment of myocardial and cerebral ischemia.However,the effects of puerarin on neuropathic pain are still unclear.In this study,a neuropathic pain animal model was created by partial sciatic nerve ligation.Puerarin（30 or 60 mg/kg） was intraperitoneally injected once a day for 7 days.Mechanical allodynia and thermal hyperalgesia were examined at 1 day after model establishment.Mechanical threshold and paw withdrawal latency markedly increased in a dose-dependent manner in puerarin-treated rats,especially at 7 days after model establishment.At 7 days after model establishment,quantitative real-time reverse transcriptase-polymerase chain reaction results showed that puerarin administration reversed m RNA expression of transient receptor potential vanilloid 1（Trpv1） and transient receptor potential ankyrin 1（Trpa1） in a dose-dependent manner in dorsal root ganglion neurons after peripheral nerve injury.These results suggest that puerarin dose-dependently ameliorates neuropathic pain by suppressing Trpv1 and Trpa1 up-regulation in dorsal root ganglion of neuropathic pain rats.展开更多
地外空间的亚磁场环境是影响宇航员健康的一种潜在风险因素.动物和人体实验表明,亚磁场显著影响个体行为和神经系统功能.但是,目前尚缺乏亚磁场对动物行为和生理等多方面影响的系统检测数据.本文构建了一个适用于动物饲养的亚磁场环境（...地外空间的亚磁场环境是影响宇航员健康的一种潜在风险因素.动物和人体实验表明,亚磁场显著影响个体行为和神经系统功能.但是,目前尚缺乏亚磁场对动物行为和生理等多方面影响的系统检测数据.本文构建了一个适用于动物饲养的亚磁场环境（〈500 n T）,并系统检测了30天亚磁场处理对成年雄鼠（C57BL/6,4~6周龄,（20±2）g）的昼夜周期、痛觉、情绪及激素水平的影响.实验结果表明,对比地磁场中饲喂对照组,亚磁场中小鼠的昼夜饮水节律改变、热敏痛觉耐受能力和整体活动水平降低,但是没有发生焦虑或抑郁情绪.亚磁场处理后,小鼠血清去甲肾上腺素水平显著下降.这些结果说明一个月连续亚磁场处理扰乱动物的昼夜活动节律和内分泌,随后可能导致其感知觉能力的变化和运动机能的下降.展开更多
目的：观察豆腐果苷（helicid）对坐骨神经慢性压迫伤（CCI）后大鼠热痛觉过敏的影响及对脊髓背角磷酸化cAMP反应元件结合蛋白（phosphorylated cAMP response element binding protein,p-CREB）表达的影响。方法：32只180～220 g Wista...目的：观察豆腐果苷（helicid）对坐骨神经慢性压迫伤（CCI）后大鼠热痛觉过敏的影响及对脊髓背角磷酸化cAMP反应元件结合蛋白（phosphorylated cAMP response element binding protein,p-CREB）表达的影响。方法：32只180～220 g Wistar雄性大鼠随机分为4组（n=8）;分别为Sham组（假手术组）、CCI组（CCI模型组）、H1组（CCI 10天后豆腐果苷,25 mg·kg^-1·d^-1×10 d灌胃）和H2组（CCI 10天后豆腐果苷,50 mg·kg^-1·d^-1×10d灌胃）。4组分别于CCI手术前1天、CCI手术后1、3、5、7、9、11、12、13、14、15、16、17、18、19、20天进行热板实验、测后肢回缩时间（PWL）。CCI后20天取L4、5脊髓,免疫组织化学染色,计数p-CREB免疫反应阳性（p-CREB-IR）神经元数量。结果：第一部分：CCI手术后第9天,Sham组、CCI组、H1组、H2组分别与术前PWL基础值比较,Sham组无差异（P〉0.05）,CCI组、H1组、H2组均有差异（P〈0.05）,且下降达30（以上;CCI手术后第9天,CCI组、H1组、H2组分别做PWL值组间两两比较均无差异（P〉0.05）,证实CCI模型制作成功。给药后第1～10天,H1组、H2组分别与CCI组做组间比较,PWL值增高,H2组第3天已有差异,H1组第5天开始有差异,两组PWL值增高至第7天达峰值,后一直保持稳定,H2组较H1组保持较高水平。第二部分：脊髓背角p-CREB免疫反应阳性神经元细胞计数比较：与Sham组比较,CCI组、H1组和H2组计数明显升高,有差异（P〈0.01）;H1组和H2组分别与CCI组比较,数量较之为少（P〈0.01）;H1组计数稍大于H2组,但无差异;即CCI〉H1〉H2〉Sham组。结论：口服豆腐果苷能剂量依赖性提高CCI大鼠热痛阈值,降低热痛觉过敏,并与影响脊髓背角p-CREB的表达有关。展开更多
目的:探讨大鼠背根神经节慢性压迫CCD后瞬时感受器电位离子通道香草素受体4(TRPV4)下游信号分子及其在痛觉过敏中的机制。方法:鞘内分别注射TRPV4拮抗剂钌红(RR)、TRPV4反义寡脱氧核苷酸(ASODN)和一氧化氮合成酶(NOS)抑制剂L-NAME,检测...目的:探讨大鼠背根神经节慢性压迫CCD后瞬时感受器电位离子通道香草素受体4(TRPV4)下游信号分子及其在痛觉过敏中的机制。方法:鞘内分别注射TRPV4拮抗剂钌红(RR)、TRPV4反义寡脱氧核苷酸(ASODN)和一氧化氮合成酶(NOS)抑制剂L-NAME,检测CCD大鼠背根神经节DRG内一氧化氮(NO)代谢产物亚硝酸盐(nitrite)含量变化,并观测热刺激缩爪反应潜伏期(PWL)的变化。结果:鞘内分别注射RR、TRPV4 AS ODN和L-NAME后,均能够显著降低CCD大鼠DRG内亚硝酸盐含量(P<0.05),CCD大鼠的热痛敏行为也能够显著改善(P<0.05)。结论:TRPV4及其下游信号分子NO参与介导CCD大鼠的热痛觉过敏。展开更多
文摘Aim: Diabetic neuropathic pain is one of the pains which hardly respond to pharmaceutical treat. Today, various chemical and herbal compounds have been used to reduce pain. The aim of this study is to compare the effect of date extract and melatonin in preventing pain in diabetic rats.Method: To study hyperalgesia response and to compare the effect of date extract and melatonin in preventing pain, hot plate and tail flick tests were used. After prescribing single dose of streptozotocin to rats and approving their diabetes, treatment rats received date extract (4ml/kg/day) or melatonin [10 mg/kg/day, intraperitoneally (i.p.)] for a period of 6 weeks. At the end of the sixth week, control and treated rats were examined by thermal pain response and explorative activity tests.Results: According to hot plate results, response time to thermal pain in treated group showed a significant decrease in comparison with the control group (P 0.01). Prescription of date extract increased response time to thermal pain in comparison with treated group (P 0.01), so that response time approximated to control group. Although melatonin approximated to the response time to control group, the significant difference was not observed among melatonin receivers and other groups. In the assessment of diabetic neuropathy on the explorative activity of rats in an open field behavioral test, total distance moved and rearing frequency were significantly decreased, while administration of date extract did also improve motor deficits induced by STZ. Conclusions:Findings of this study showed that date extract decreased thermal hyperalgesia and can prevent pain resulted from diabetic neuropathy.
基金supported by the National Natural Science Foundation of China,No.81671891
文摘Puerarin is a major active ingredient of the traditional Chinese plant medicine,Radix Puerariae,and commonly used in the treatment of myocardial and cerebral ischemia.However,the effects of puerarin on neuropathic pain are still unclear.In this study,a neuropathic pain animal model was created by partial sciatic nerve ligation.Puerarin（30 or 60 mg/kg） was intraperitoneally injected once a day for 7 days.Mechanical allodynia and thermal hyperalgesia were examined at 1 day after model establishment.Mechanical threshold and paw withdrawal latency markedly increased in a dose-dependent manner in puerarin-treated rats,especially at 7 days after model establishment.At 7 days after model establishment,quantitative real-time reverse transcriptase-polymerase chain reaction results showed that puerarin administration reversed m RNA expression of transient receptor potential vanilloid 1（Trpv1） and transient receptor potential ankyrin 1（Trpa1） in a dose-dependent manner in dorsal root ganglion neurons after peripheral nerve injury.These results suggest that puerarin dose-dependently ameliorates neuropathic pain by suppressing Trpv1 and Trpa1 up-regulation in dorsal root ganglion of neuropathic pain rats.
基金supported by grants from the project of Chinese Academy of Sciences for the development of major scientific research equipment(YZ201148)The National Natural Science Foundation of China(31200628)+1 种基金the External Cooperation Program of BIC,Chinese Academy of Sciences(GJHZ201302)the Queensland-Chinese Academy of Sciences Biotechnology Fund(GJHZ1131)~~
文摘地外空间的亚磁场环境是影响宇航员健康的一种潜在风险因素.动物和人体实验表明,亚磁场显著影响个体行为和神经系统功能.但是,目前尚缺乏亚磁场对动物行为和生理等多方面影响的系统检测数据.本文构建了一个适用于动物饲养的亚磁场环境（〈500 n T）,并系统检测了30天亚磁场处理对成年雄鼠（C57BL/6,4~6周龄,（20±2）g）的昼夜周期、痛觉、情绪及激素水平的影响.实验结果表明,对比地磁场中饲喂对照组,亚磁场中小鼠的昼夜饮水节律改变、热敏痛觉耐受能力和整体活动水平降低,但是没有发生焦虑或抑郁情绪.亚磁场处理后,小鼠血清去甲肾上腺素水平显著下降.这些结果说明一个月连续亚磁场处理扰乱动物的昼夜活动节律和内分泌,随后可能导致其感知觉能力的变化和运动机能的下降.
文摘目的:探讨大鼠背根神经节慢性压迫CCD后瞬时感受器电位离子通道香草素受体4(TRPV4)下游信号分子及其在痛觉过敏中的机制。方法:鞘内分别注射TRPV4拮抗剂钌红(RR)、TRPV4反义寡脱氧核苷酸(ASODN)和一氧化氮合成酶(NOS)抑制剂L-NAME,检测CCD大鼠背根神经节DRG内一氧化氮(NO)代谢产物亚硝酸盐(nitrite)含量变化,并观测热刺激缩爪反应潜伏期(PWL)的变化。结果:鞘内分别注射RR、TRPV4 AS ODN和L-NAME后,均能够显著降低CCD大鼠DRG内亚硝酸盐含量(P<0.05),CCD大鼠的热痛敏行为也能够显著改善(P<0.05)。结论:TRPV4及其下游信号分子NO参与介导CCD大鼠的热痛觉过敏。
基金This project was supported by the National Natural Science Foundation of China (No. 39970925 ) , the Research Fund for Doctoral Program of Higher Education ( No. 9835).
