A series of hydrogels with lower critical solution temperature (LCST) were prepared and used for the immobilization of chymotrypsin. The activities of the immobilized enzyme decrease when the temperatures are raised a...A series of hydrogels with lower critical solution temperature (LCST) were prepared and used for the immobilization of chymotrypsin. The activities of the immobilized enzyme decrease when the temperatures are raised above their LCST and recover below their LCST. This property is reversible.展开更多
A novel immunoassay method for methyltestosterone (MT) in serum ovas developed.The antigen of MT (Ag(MT))was synthesized by covalently bonding MT to BSA and raising its antibody (Ab(MT)). Ab(MT) was con.jugated to pol...A novel immunoassay method for methyltestosterone (MT) in serum ovas developed.The antigen of MT (Ag(MT))was synthesized by covalently bonding MT to BSA and raising its antibody (Ab(MT)). Ab(MT) was con.jugated to poly-N-isopropylacrylamide (p-NIPAAm) to form Ab(MT) bound thermally reversible hydrogel p-NIPAAm-Ab((MT) A competitive immunoassay method based on the competition of fluorescein isothiocyanate (FITC) labeled MT antigen (FITC-Ag(MT)) and free MT with limited amount of p-NIPAAm-Ab(MT) was established. The separation in the assay process was achieved by precipitation of the immuno-complex above its critical solution temperature. The detection limit for MT is 50ng/ml. The recoveries of MT from human serum are satisfactory.展开更多
文摘A series of hydrogels with lower critical solution temperature (LCST) were prepared and used for the immobilization of chymotrypsin. The activities of the immobilized enzyme decrease when the temperatures are raised above their LCST and recover below their LCST. This property is reversible.
文摘A novel immunoassay method for methyltestosterone (MT) in serum ovas developed.The antigen of MT (Ag(MT))was synthesized by covalently bonding MT to BSA and raising its antibody (Ab(MT)). Ab(MT) was con.jugated to poly-N-isopropylacrylamide (p-NIPAAm) to form Ab(MT) bound thermally reversible hydrogel p-NIPAAm-Ab((MT) A competitive immunoassay method based on the competition of fluorescein isothiocyanate (FITC) labeled MT antigen (FITC-Ag(MT)) and free MT with limited amount of p-NIPAAm-Ab(MT) was established. The separation in the assay process was achieved by precipitation of the immuno-complex above its critical solution temperature. The detection limit for MT is 50ng/ml. The recoveries of MT from human serum are satisfactory.
