Characteristics and main controlling factors of intra-platform shoal thin-layer dolomite reservoirs:A case study of Middle Permian Qixia Formation in Gaoshiti-Moxi area of Sichuan Basin,SW China

Based on the study of the distribution of intra-platform shoals and the characteristics of dolomite reservoirs in the Middle Permian Qixia Formation in the Gaoshiti–Moxi area of the Sichuan Basin,SW China,the controlling factors of reservoir development were analyzed,and the formation model of“intra-platform shoal thin-layer dolomite reservoir”was established.The Qixia Formation is a regressive cycle from bottom to top,in which the first member(Qi1 Member)develops low-energy open sea microfacies,and the second member(Qi2 Member)evolves into intra-platform shoal and inter-shoal sea with decreases in sea level.The intra-platform shoal is mainly distributed near the top of two secondary shallowing cycles of the Qi2 Member.The most important reservoir rock of the Qixia Formation is thin-layer fractured-vuggy dolomite,followed by vuggy dolomite.The semi-filled saddle dolomite is common in fracture-vug,and intercrystalline pores and residual dissolution pores combined with fractures to form the effective pore-fracture network.Based on the coupling analysis of sedimentary and diagenesis characteristics,the reservoir formation model of“pre-depositional micro-paleogeomorphology controlling shoal,sedimentary shoal controlling dolomite,penecontemporaneous dolomite benefiting preservation of pores,and late hydrothermal action effectively improving reservoir quality”was systematically established.The“first-order high zone”micro-paleogeomorphology before the deposition of the Qixia Formation controlled the development of large area of intra-platform shoals in Gaoshiti area during the deposition of the Qi2 Member.Shoal facies is the basic condition of early dolomitization,and the distribution range of intra-platform shoal and dolomite reservoir is highly consistent.The grain limestone of shoal facies is transformed by two stages of dolomitization.The penecontemporaneous dolomitization is conducive to the preservation of primary pores and secondary dissolved pores.The burial hydrothermal fluid enters the early dolomite body along the fractures associated with the Emeishan basalt event,makes it recrystallized into medium–coarse crystal dolomite.With the intercrystalline pores and the residual vugs after the hydrothermal dissolution along the fractures,the high-quality intra-platform shoal-type thin-layer dolomite reservoirs are formed.The establishment of this reservoir formation model can provide important theoretical support for the sustainable development of Permian gas reservoirs in the Sichuan Basin.

Photothermal-photocatalytic thin-layer flow system for synergistic treatment of wastewater

The integration of the photocatalytic effect into solar steam is highly desirable for addressing freshwater shortages and water pollution.Here,a ternary film structure for the adsorption and photothermal and photocatalytic treatment of wastewater was designed by combining the technique of self-assembled carbon nano paper with a nitrogen composite titanium dioxide(N-TiO_(2))deposited on the surface of carbon nanotubes(CNT)using polyvinylidene fluoride(PVDF)as a substrate.The photogeneration of reactive oxygen species can be promoted by rapid oxygen diffusion at the three-phase interface,whereas the interfacial photothermal effect promotes subsequent free radical reactions for the degradation of rhodamine B(93%).The freshwater evaporation rate is 1.35 kg·m^(-2)·h^(-1)and the solar-to-water evaporation efficiency is 94%.Importantly,the N-TiO_(2)/CNT/PVDF(N-TCP)film not only effectively resists mechanical damage from the environment and maintains structural integrity,but can also be made into a large film for outdoor experiments in a large solar energy conversion device to collect fresh water from polluted water and degrade organic dyes in source water simultaneously,opening the way for applications in energy conversion and storage.

ANALYSIS OF HUMAN PLACENTA BY ^(31)P NUCLEAR MAGNETIC RESONANCE AND THIN-LAYER CHROMATOGRAPHY SCANNING COMBINED WITH THE CORRECTIVE METHOD OF ABSORBANCE PROPORTIONAL COEFFICIENT

Five phospholipids in human placenta were determined by phosphorus 31 nuclear magnetic resonance(^(31)P NMR)spectroscopy and thin-layer chromatography(TLC) scanning combined with the corrective method of absorbance proportional coefficient. The NMR spectrometer used this investigation was a Bruker AM-500 spectrometer operating at 202.4 MHz for ^(31)P chemical shifts are relative to 85% phosphoric acid. TIC was carried out by silica gel H plate developed in chloroform-methanol-glacial acetic acid-ethanol-water(25:4:6:2:0.5),with Vaskovsky reagent as colour -developing agent of phospholipids.

Quality Evaluation of Leontopodium franchetii Beauv. from Different Localities Based on Moisture,Ash and Extract Contents and Thin-layer Chromatography

[Objectives] The aim was to determine the moisture,ash and extract contents in Leontopodium franchetii Beauv. from different localities. [Methods] The contents of moisture,total ash,acid-insoluble ash and extract in L. franchetii from different localities were determined according to the methods described in Chinese Pharmacopoeia( 2015 Edition). [Results] In the 10 samples,the contents of moisture were all less than or equal to 15. 0%; the contents of total ash were all below 12. 0%; and the contents of acid-insoluble ash were all less than 3. 0%. The contents of water-soluble extract( by cold-soaked extraction method) in the samples were all below 12. 0% except those from Hongyuan Prairie of Sichuan Province and Xinglong Mountain in Lanzhou City,Gansu Province. [Conclusions]The contents of the four indicators measured in this experiment varied by small margins,indicating that the quality was relatively stable. This study provides theoretical data for the revision of the quality standards for L. franchetii.

Gas Chromatographic Method for Identification and Quantification of Commonly Used Residual Solvents in Pharmaceuticals Products

Background: Impurities are not expected in the final pharmaceutical products. All impurities should be regulated in both drug substances and drug products in accordance with pharmacopeias and ICH guidelines. Three different types of impurities are generally available in the pharmaceutical’s product specification: organic impurities, inorganic impurities, and residual solvents. Residual solvents are organic volatile chemicals used or generated during the manufacturing of drug substances or drug products. Purpose: The aim of this study is to develop a cost-effective gas chromatographic method for the identification and quantification of some commonly used solvents—methanol, acetone, isopropyl alcohol (IPA), methylene chloride, ethyl acetate, tetrahydrofuran (THF), benzene, toluene, and pyridine—in pharmaceutical product manufacturing. This method will be able to identify and quantify the multiple solvents within a single gas chromatographic procedure. Method: A gas chromatography (GC) equipped with a headspace sampler and a flame ionization detector, and a column DB 624, 30-meter-long × 0.32-millimeter internal diameter, 1,8 μm-thick, Brand-Agilent was used to develop this method. The initial GC oven temperature was 40°C and held for 5 minutes. It was then increase to 80˚C at a rate of 2˚C per minute, followed by a further increase to 225˚C at a rate of 30˚C per minute, with a final hold at 225˚C for 10 minutes. Nitrogen was used as a carrier gas at a flow rate of 1.20 mL per minute. Dimethyl sulfoxide (DMSO) was selected as sample solvent. Results: The developed method is precise and specific. The percent RSD for the areas of six replicate injections of this gas chromatographic method was within 10.0 and the recovery result found within 80.0% to 120.0%.

Separation of Amino Acids Based on Thin-Layer Chromatography by a Novel Quinazoline Based Anti-Microbial Agent

A newly designed quinazoline based compound, 6-pyridin-2-yl-5,6-dihydro-benzo[4,5]imidazo[1,2-c] quinazoline (PDBIQ) has shown the ability for the easy detection of nineteen amino acids on thin-layer chromatography plates as a spray reagent. This new reagent enabled to produce various distinguishable colors with amino acids with different RF values. The detection limits and the binding ability of PDBIQ with amino acids have been calculated. PDBIQ is also able to detect aminoacids from hydrolised seed protein. The title compound also exhibited profound inhibitory action against some gm (+ve) and gm (-ve) bacterial organisms. This paper deals with synthesis, spectroscopic application and biological evaluation of the organic moity.

Simultaneous purification of minor components in natural products using twin-column recycling chromatography with a step solvent gradient

The isolation of minor components from complex natural product matrices presents a significant challenge in the field of purification science due to their low concentrations and the presence of structurally similar compounds.This study introduces an optimized twin-column recycling chromatography method for the efficient and simultaneous purification of these elusive constituents.By introducing water at a small flowing rate between the twin columns,a step solvent gradient is created,by which the leading edge of concentration band would migrate at a slower rate than the trailing edge as it flowing from the upstream to downstream column.Hence,the band broadening is counterbalanced,resulting in an enrichment effect for those minor components in separation process.Herein,two target substances,which showed similar peak position in high performance liquid chromatography(HPLC)and did not exceed 1.8%in crude paclitaxel were selected as target compounds for separation.By using the twin-column recycling chromatography with a step solvent gradient,a successful purification was achieved in getting the two with the purity almost 100%.We suggest this method is suitable for the separation of most components in natural produces,which shows higher precision and recovery rate compared with the common lab-operated separation ways for natural products(thin-layer chromatography and prep-HPLC).

High throughput and rapid isolation of extracellular vesicles and exosomes with purity using size exclusion liquid chromatography

Extracellular vesicles(EVs)have emerged as potential biomarkers for diagnosing a range of diseases without invasive procedures.Extracellular vesicles also offer advantages compared to synthetic vesicles for delivery of various drugs;however,limitations in segregating EVs from other particles and soluble proteins have led to inconsistent EV retrieval rates with low levels of purity.Here,we report a new high-yield(88.47%)and rapid(<20 min)EV isolation method termed size exclusion–fast protein liquid chromatography(SE-FPLC).We show SE-FPLC can effectively isolate EVs from multiple sources including EVs derived from human and mouse cells and serum samples.The results indicate that SE-FPLC can successfully remove highly abundant protein contaminants such as albumin and lipoprotein complexes,which can represent a major hurdle in large scale isolation of EVs.The high-yield nature of SE-FPLC allows for easy industrial scaling up of EV production for various clinical utilities.SE-FPLC also enables analysis of small volumes of blood for use in point-of-care diagnostics in the clinic.Collectively,SE-FPLC offers many advantages over current EV isolation methods and offers rapid clinical translation.

Simple and Rapid Thin-layer Chromatography Method for Quantitative Measurement of Free Cholesterol in Serum

ABSTRACT A quantitative method for the assay of free cholesterol has been described in this paper. The experimental conditions for the determination of cholesterol in serum by Thin-layer chromatography were disscused. The solvent System was petroleum ether-ethyl acetate-glacial acetic acid (8o:20:1) and the spra-ying reagent was a solution of sulphuric acid and vanillin. Under the selected con-ditions, the peak area was linearly related to the cholesterol amount for the range between 80～700 ng per spot. The intraplate and interplate coefficients were 2.4% and 7.4% respectively. The recovery of cholesterol was 101.6%. The method presented was simple, rapid and accurate. The results of experi-mental investigation and clinical application were satisfactory.

Stability indicating high performance thin-layer chromatographic method for simultaneous estimation of pantoprazole sodium and itopride hydrochloride in combined dosage form

A specific, precise and stability indicating high-performance thin-layer chromatographic method for simultaneous estimation of pantoprazole sodium and itopride hydrochloride in pharmaceutical formulations was developed and validated. The method employed TLC aluminium plates precoated with silica gel 60F254 as the stationary phase. The solvent system consisted of methanol：water：ammonium acetate; 4.0：1.0：0.5 （v/v/v）. This system was found to give compact and dense spots for both itopride hydrochloride （Rf value of 0.55__＋0.02） and pantoprazole sodium （Rf value of 0.85＋0.04）. Densitometric analysis of both drugs was carried out in the reflectance- absorbance mode at 289 nm. The linear regression analysis data for the calibration plots showed a good linear relationship with R2=0.9988___0.0012 in the concentration range of 100--400 ng for pantoprazole sodium. Also, the linear regression analysis data for the calibration plots showed a good linear relationship with R2=0.9990_＋0.0008 in the concentration range of 200-1200 ng for itopride hydrochloride. The method was validated for specificity, precision, robustness and recovery. Statistical analysis proves that the method is repeatable and selective for the estimation of both the said drugs. As the method could effectively separate the drug from its degradation products, it can be employed as a stability indicating method.

Determination of 14 Organophosphorus Pesticide Residues in Mutton by Gel Permeation Chromatography-Gas Chromatography-Mass Spectrometry(GPC-GC-MS)

[Objectives]This study was conducted to purify mutton samples by gel permeation chromatography(GPC).[Methods]Fourteen organophosphorus pesticide residues in samples were qualitatively and quantitatively analyzed by gas chromatography-mass spectrometry(GC-MS)in selective ion scanning mode(SIM).[Results]The organophosphorus pesticide standard solutions showed good linearity in the mass concentration range of 0.1-10.0μg/ml with correlation coefficients(r)not lower than 0.999,and the detection limits(S=3 N)ranged from 0.01 to 0.05 mg/kg.The average recovery values were in the range of 80.2%-99.7%,with relative standard deviations(RSDs,n=3)in the range of 1.8%-6.3%,at the addition levels of 0.5,1.0 and 2.0 mg/kg.[Conclusions]The method is simple,sensitive and accurate,and can be used for the determination of organophosphorus pesticide residues in mutton.

Comprehensive analysis of phenolic composition and antioxidant mechanisms in Gymnema sylvestre extracts using LC-MS and column chromatography

Recent studies have highlighted the potential of plant extracts as therapeutic agents for managing oxidative stress and related disorders.This study aims to elucidate the phenolic composition and antioxidant properties of Gymnema sylvestre extracts.Ethanolic reflux extraction followed by column chromatography was employed to isolate phenolic compounds.The total phenolic and flavonoid contents were quantified using the Folin–Ciocalteu and aluminum chloride colorimetric methods,respectively.Antioxidant activities were assessed by DPPH,ABTS scavenging assays and the ferric reducing antioxidant power(FRAP)assay.High-Performance Liquid Chromatography(HPLC)with a C18 column and Thermo TSQ Quantum Access Max(LC-MS)were used to determine the levels of gymnemic acid and identify other potential phenolic compounds.The analysis revealed significant antioxidant activities in the fractions.Fraction A showed the highest DPPH and ABTS scavenging activities,and Fraction C demonstrated the highest ferric reducing power.LC-MS analysis identified several phenolic compounds,indicating that these are major contributors to the antioxidant efficacy of the extract.This study provides a detailed phenolic profile and confirms the strong antioxidant potential of Gymnema sylvestre leaf extract,supporting its therapeutic use and further investigation.

Thin-Layer Chromatographic Separation of Amino Acid Enantiomers using Ligand Exchange

Silica gel thin-layer plates covered with L-arginine and copper acetate were used for the separation of amino acid enantiomers, The chromatographic selectivity and the effects of plate different preparation methods, sample molecular structure and solvent compositions on resolution performance were also discussed.

Determination of Benzo[a]pyrene in Edible Oil by High Performance Liquid Chromatography-Fluorescence Detector (HPLC-FL)

In this study, an optimized high performance liquid chromatography-fluorescence detector (HPLC-FL) method for the determination of benzo[a]pyrene in edible oil was established. HPLC was performed with Thermo Fisher Scientific C18 column (250 mm×4.6 mm, 5 μm) as the chromatographic column and acetonitrile and water as the mobile phase, and the excitation wavelength and emission wavelength of fluorescence detector were 286 and 430 nm, respectively. The response was high, and the linear range was 0.5-10.0 ng/ml. The lowest limit of detection was 0.11 ng/ml, and the average recovery was 92.5%. This method is suitable for quantitative analysis of benzo[a]pyrene content in edible oil.

ANALYSIS OF STRYCHNINE ALKALOIDS IN BIOLOGICAL SAMPLES BY THIN-LAYER CHROMATOGRAPHIC DENSITOMETRY AND DISTRIBUTION STUDY OF STRYCHNINE IN INTOXICATED RATS

A sensitive and rapid analytical procedure was established to detrmine strychnie alkaloids in diverse biological specimens. Samples were treated with hydrochloric acid to precipitate protein and scanned in thin-layer chromatograpic scanner in zig-zag dual-wavelength reflection mode.Detection sensitivity for strychniue was about 0. 05 μg. Quantification recoveries were between89. 2% and 99. 5% with RSD between 2. 92% and 13. 21 %. The method was applied to investigate thedistribution or strychnine in intoxicated rats. The results showed that there were remarkable difference in organs or tissues with the aigaest concentration 4. 56±1. 41μg/g in liver. Diversification experiment proves the method also could be used to analyze other alkaloids in biological samples if theconditions slightly modified.

A review of SAGD technology development and its possible application potential on thin-layer super-heavy oil reservoirs

Super-heavy oil is a significant unconventional energy source,and more than 30 years of research have shown that steam-assisted gravity drainage(SAGD)technology is suitable for thick super-heavy oil reservoirs.Recently,more and more thin-layer super-heavy oil reservoirs have been discovered in China,while their deep buried depth and serous heterogeneity make the existing SAGD technology difficult to apply,so it is urgent to improve the existing SAGD technology for the thin-layer super-heavy oil.To this end,this paper focuses on the enlightenment of field application in SAGD technology.Firstly,based on typical SAGD field projects,the development history of SAGD technology in the world was reviewed,and the influence of reservoir physical properties on the application of SAGD technology in thin-layer superheavy oil reservoirs was analyzed.Secondly,the well pattern,wellbore structure,pre-heating,artificial lift,and monitor technique of SAGD were detailed described,and their adjustment direction was expounded for the development of thin-layer super-heavy oil reservoirs.Lastly,the gas-and solventassistant SAGD were comprehensively evaluated,and their application potential in thin-layer superheavy oil reservoirs was studied.The research results can provide theoretical guidance for the application of SAGD technology in thin-layer super-heavy oil reservoirs.

Study on microwave-induced thin-layer drying of municipal sludge for fuel reclamation

Since the adverse factors such as deficient penetration and long reaction time have restricted the complete microwave-used drying of municipal sludge, the microwave-induced drying is considered, which has advantages in such aspects. The investigation of the microwave-induced drying to uncover the mechanism bears great meaning for its development and utilization. The effects of temperature and microwave cracking in municipal sewage sludge drying characteristics are stud- ied through municipal sewage sludge drying experiment. Experiments shows that higher drying temperature would lead to a more acutely changing drying rate （DR）. The DR had increased from 0.005 g/（g.min） to 0.060 g/（g·min）, which was 12 times enlarged, while the temperature rose from 70℃ to 160 ℃. The higher the temperature was, the earlier the peak value of DR appeared. The experiments indicated that the temperature was the decisive factor affecting the DR. The microwave-induced sludge reached the highest DR at the moisture rate （MR） of 40%, with a 20% grade promotion compared with that of the original one. The molecular fracture caused by microwave radiation had obviously accelerated the drying process and the DR was rising in proportion to the microwave radiation dose. The diffusion coefficient had been calculated according to Fick＇s law. In com- parison to that of the original one, the diffusion coefficient of microwave-induced sludge was obviously enlarged more than two times. By fit examinations, Model Weibull was proven to be the most fit one for thermal thin-layer drying of municipal sludge. By means of Arrhenius equation, the obtained average activation energy of municipal sludge was 37.1 kJ/（mol·K）.

Determination of Organic Acids in Root Exudates by High Performance Liquid Chromatography: I. Development and Assessment of Chromatographic Conditions *1

Methods for determining nine low molecular weight organic acids in root exudates were developed by using reversed phase high performance liquid chromatography with UV (ultraviolet) detection at 214 nm. The mobile phase was 18 mmol L -1 kH 2PO 4 adjusted to pH 2.25 with phosphoric acid and the flow rate was 0.3 mL min -1 . The analytical column was a reversed phase silica based C 18 column (Shim pack CLC ODS). The root exudates were collected through submerging the whole root system into aerated deionized water for 2 hours. The filtered exudate solutions were concentrated to dryness by rotary evaporation at 40 °C, dissolved in 10 mL mobile phase. The chromatographic conditions of organic acid determination were analyzed. The results showed that there was a high selectivity and sensitivity in the organic acid determination by reversed phase high performance liquid chromatography. Coefficients of variation for organic acid determination were lower than 10% except lactic acid. The recoveries were consistently between 80.1% to 108.3%. Detection limits were approximately 0.05 to 4.5 mg L -1 for organic acids except succinic acid with the detection limit of 7.0 mg L -1 . Phosphorus deficiency may contribute to the release of organic acids in soybean root exudates especially malic, lactic and citric acids.

APPLICATIONS AND MANUFACTURE OF THE MICROSCALE LONG-OPTICAL-PATH ELECTROCHEMICAL CELL WITH A PLUG-IN THIN-LAYER ELECTRODE

The construction and characteristics of a microscale long-optical-path electrochemi- cal cell with a plug-in thin-layer electrode are described.Using ferricyanide as the test species,the thermodynamic parameters of electron transfer processes are determined at car- bon,plantinum,and gold electrodes.

Efficient and selective extraction of uranium from seawater based on a novel pulsed liquid chromatography radionuclide separation method

The novel pulsed liquid chromatography radionuclide separation method presented here provides a new and promising strategy for the extraction of uranium from seawater.In this study,a new chromatographic separation method was proposed,and a pulsed nuclide automated separation device was developed,alongside a new chromatographic column.The length of this chromatographic column was 10 m,with an internal warp of 3 mm and a packing size of 1 mm,while the total separation units of the column reached 12,250.The most favorable conditions for the separation of nuclides were then obtained through optimizing the separation conditions of the device:Sample pH in the column=2,sample injection flow rate=5.698 mL/min,chromatographic column heating temperature=60℃.Separation experiments were also carried out for uranium,europium,and sodium ions in mixed solutions;uranium and sodium ions in water samples from the Ganjiang River;and uranium,sodium,and magnesium ions from seawater samples.The separation factors between the different nuclei were then calculated based on the experimental data,and a formula for the separation level was derived.The experimental results showed that the separation factor in the mixed solution of uranium and europium(1:1)was 1.088,while achieving the initial separation of uranium and europium theoretically required a 47-stage separation.Considering the separation factor of 1.50for the uranium and sodium ions in water samples from the Ganjiang River,achieving the initial separation of uranium and sodium ions would have theoretically required at least a 21-stage separation.Furthermore,for the seawater sample separation experiments,the separation factor of uranium and sodium ions was 1.2885;therefore,more than 28 stages of sample separation would be required to achieve uranium extraction from seawater.The novel pulsed liquid chromatography method proposed in this study was innovative in terms of uranium separation and enrichment,while expanding the possibilities of extracting uranium from seawater through chromatography.