Detection of Novel BEST1 Variations in Autosomal Recessive Bestrophinopathy Using Third-generation Sequencing

Objective:Autosomal recessive bestrophinopathy(ARB),a retinal degenerative disease,is characterized by central visual loss,yellowish multifocal diffuse subretinal deposits,and a dramatic decrease in the light peak on electrooculogram.The potential pathogenic mechanism involves mutations in the BEST1 gene,which encodes Ca2+-activated Cl−channels in the retinal pigment epithelium(RPE),resulting in degeneration of RPE and photoreceptor.In this study,the complete clinical characteristics of two Chinese ARB families were summarized.Methods:Pacific Biosciences(PacBio)single-molecule real-time(SMRT)sequencing was performed on the probands to screen for disease-causing gene mutations,and Sanger sequencing was applied to validate variants in the patients and their family members.Results:Two novel mutations,c.202T>C(chr11:61722628,p.Y68H)and c.867+97G>A,in the BEST1 gene were identified in the two Chinese ARB families.The novel missense mutation BEST1 c.202T>C(p.Y68H)resulted in the substitution of tyrosine with histidine in the N-terminal region of transmembrane domain 2 of bestrophin-1.Another novel variant,BEST1 c.867+97G>A(chr11:61725867),located in intron 7,might be considered a regulatory variant that changes allele-specific binding affinity based on motifs of important transcriptional regulators.Conclusion:Our findings represent the first use of third-generation sequencing(TGS)to identify novel BEST1 mutations in patients with ARB,indicating that TGS can be a more accurate and efficient tool for identifying mutations in specific genes.The novel variants identified further broaden the mutation spectrum of BEST1 in the Chinese population.

The Influences of American Deep Image on the Third-Generation Poets in China

The Third-Generation Poetry of China （namely Post-misty Poetry too） initiated with the introduction of Western modernist poetry, especially sorts of American Post-modernist poetry schools into China. ＂The relation between American poetry and Chinese poetry has a long history, which lies in the influences on the creation of the Third-Generation poets. This influence is probably unprecedented in its depth and breadth.＂ ＂Irrational association＂ and ＂leaping images＂ proposed by American Deep Image poets influenced by Freudian and Jungian unconscious perception gained an extraordinary appreciation among the Third-Generation poets who were in pursuit constantly of the experiments on poetic form and language. This paper mainly discusses the influences of American Deep Image on the Third-Generation poets of China through a case study of WANG Yin and CHEN Dongdong＇s poems.

Recent progress in third-generation low alloy steels developed under M^3 microstructure control

During the past thirty years, two generations of low alloy steels(ferrite/pearlite followed by bainite/martensite) have been developed and widely used in structural applications. The third-generation of low alloy steels is expected to achieve high strength and improved ductility and toughness, while satisfying the new demands for weight reduction, greenness, and safety. This paper reviews recent progress in the development of third-generation low alloy steels with an M^3 microstructure, namely, microstructures with multi-phase, meta-stable austenite, and multi-scale precipitates. The review summarizes the alloy designs and processing routes of microstructure control, and the mechanical properties of the alloys.The stabilization of retained austenite in low alloy steels is especially emphasized. Multi-scale nano-precipitates, including carbides of microalloying elements and Cu-rich precipitates obtained in third-generation low alloy steels, are then introduced. The structure–property relationships of third-generation alloys are also discussed. Finally, the promises and challenges to future applications are explored.

Innovation and development of the third-generation hybrid rice technology

The breeding and large-scale application of hybrid rice contribute significantly to the food supply worldwide.Currently,hybrid seed production uses cytoplasmic male sterile(CMS)lines or photoperiod/thermo-sensitive genic male sterile(PTGMS)lines as female parent.Despite huge successes,both systems have intrinsic problems.CMS systems are mainly restricted by the narrow restorer resources that make it difficult to breed superior hybrids,while PTGMS systems are limited by conditional sterility of the male sterile lines that makes the propagation of both PTGMS seeds and hybrid seeds vulnerable to unpredictable climate changes.Recessive nuclear male sterile(NMS)lines insensitive to environmental conditions are widely distributed and are ideal for hybrid rice breeding and production,but the lack of effective ways to propagate the pure NMS lines in a large scale renders it impossible to use them for hybrid rice production.The development of"the third-generation hybrid rice technology"enables efficient propagation of the pure NMS lines in commercial scale.This paper discusses the establishment of"the thirdgeneration hybrid rice technology"and further innovations.This new technology breaks the limitations of CMS and PTGMS systems and will bring a big leap forward in hybrid rice production.

Applications of sediment sudden deposition model based on the third-generation numerical model for shallow water wave

The existing numerical models for nearshore waves are briefly introduced, and the third-generation numerical model for shallow water wave, which makes use of the most advanced productions of wave research and has been adapted well to be used in the environment of seacoast, lake and estuary area, is particularly discussed. The applied model realizes the significant wave height distribution at different wind directions. To integrate the model into the coastal area sediment, sudden deposition mechanism, the distribution of average silt content and the change of sediment sudden deposition thickness over time in the nearshore area are simulated. The academic productions can give some theoretical guidance to the applications of sediment sudden deposition mechanism for stormy waves in the coastal area. And the advancing directions of sediment sudden deposition model are prospected.

Development of the Mechanical System on a Third-Generation Industrial Computed Tomography Scanner in Brazil

The development of measurement geometry for medical X-ray computed tomography （CT） scanners was carried out from the first to the fourth-generation. This concept has also been applied for imaging of industrial processes such as pipe flows or for improving design, operation, optimization and troubleshooting. Nowadays, gamma CT permits to visualize failure equipment points in three-dimensional analysis and in sections of chemical and petrochemical industries. The aim of this work is the development of the mechanical system on a third-generation industrial CT scanner to analyze laboratorial process columns which perform highly efficient separation, turning the ^6oCo, ^75Se, ^137Cs and/or ^192Ir sealed gamma-ray source（s） and the NaI（Tl） multidetector array. It also has a translation movement along the column axis to obtain as many slices of the process flow as needed. The mechanical assembly for this third-generation industrial CT scanner is comprised by strength and rigidity structural frame in stainless and carbon steels, rotating table, source shield and collimator with pneumatic exposure system, spur gear system, translator, rotary stage, drives and stepper motors. The use of suitable spur gears has given a good repeatability and high accuracy in the degree of veracity. The data acquisition boards, mechanical control interfaces, software for movement control and image reconstruction were specially development. A multiphase phantom capable to be setting with solid, liquid and gas was testing. The scanner was setting for 90 views and 19 projections for each detector totalizing 11,970 projections. Experiments to determine the linear attenuation coefficients of the phantom were carried out which applied the Lambert-Beer principle. Results showed that it was possible to distinguish between the phases even the polymethylmethacrylate and the water have very similar density and linear attenuation coefficients. It was established that the newly developed third-generation fan-beam arrangement gamma scanner unit has a good spatial resolution acceptable given the size of the used phantom in this study. The tomografic reconstruction algorithm in used 60 ~ 60 pixels images was the Alternative Minimization （AM） technique and was implemented in MATLAB and VB platforms. The mechanical system presented a good performance in terms of strength, rigidity, accuracy and repeatability with great potential to be used for education or program which dedicated to training chemical and petrochemical industry professionals and for industrial process optimization in Brazil.

Comparative Analysis of Metaphors of “Flowers” in English and Chinese Poetry from the Perspective of Cognitive Metaphor Theory

Poetry,as a crucial form of literary expression,often employs metaphor as a common rhetorical device.According to the perspective of cognitive metaphor theory,metaphor transcends its traditional linguistic boundaries and is recognized as a profound cognitive mechanism,manifesting as a broader cognitive phenomenon.This article is based on metaphorical examples of“flowers”in Chinese and English poetry,carefully selecting representative cases for in-depth analysis.The aim is to compare the imagery of“flowers”in Chinese and English poetry,observe their similarities and differences,thereby fostering a better understanding of poetry in both languages.Through this study,we not only delve into the intricacies of metaphor within poetry but also shed light on the distinct interpretations of the symbol of“flowers”in different cultural contexts,expanding our appreciation for the cultural diversity inherent in poetry.

On the Translatability of Poetry

This paper discusses some reasons for the translatability of poetry since throughout the history of translation many theorists and scholars advocate that poetry is translatable.The“common core”shared by people speaking different languages,the theoretical basis to define translation correctly,and historical facts of poetry translation all lend support to the point that translation of poetry is possible.

Mastering Metaphors: A Possible Path to Understand Modern Poetry

Understanding modern poetry is a big problem for average readers because of all kinds of rhetorical devices filled with them,especially metaphors,which are hard for the readers to understand clearly.Thus,grasping metaphors would provide a possible solution to enhance the understanding of modern poetry.Based on the motivation to better understand modern poetry,the paper explores the 11 mechanisms of metaphors in modern poetry with examples in detail,i.e.,blending,mapping,frame shifting,image schema,conceptual integration,contextual grounding,inter-subjectivity,embodied cognition,recursiveness,juxtaposition,shape-moulding,by borrowing some concepts from Cognitive Linguistics,literary studies,and Rhetorics,which can be adopted as means and methods to understand modern poems.

The Early Love Poetry of W.B. Yeats——When You Are Old

When you are old is one of the most famous love poetry written by Irish Poet, William Butler Yeats. The paper elaborates deeply on one of the early love poetry of William Butler Yeats: When you are old. It gives an introduction of W.B. Yeats and then discusses Yeats' s early love poetry and his muse as well as her influence in the love poetry; Then the poem When you are old is analyzed and in the meantime the original French version and the Chinese version were provided for readers' literary appreciation.

Discussion on the Relation between Garden Landscape Constitution and Ancient Chinese Poetry and Paintings

From the perspective of culture-based source,the author considered that the ancient Chinese poetry and paintings not only embodied the harmony and unity thought of man and nature,but also were the emotional attachment of Chinese literati.As a diverse and comprehensive aesthetic art,landscape expressed material and spiritual culture doubly,the spiritual connotation of which was inextricably linked within ancient Chinese poetry and paintings.In addition,ancient Chinese poetry and paintings can be directly used in landscape composition,such as the widely used in plaques,inscriptions,couplets,poems and inscriptions,carved beams and painted pillars,painting with words and so on.Chinese literati integrated the ethereal realm of poetry and paintings into garden art,making landscape endow with poetic charm.Therefore,the landscape revealed the traces of literature and arts in aesthetic idea and taste,pushing Chinese garden to a higher aesthetic realm.This study pointed the right way to the future of modern landscape which advocated ecological and harmonious development.

Building Landscape Art with Study of Buddhism in Poetry and Drawing

Many aspects have very important effects on garden art,such as landscape poetry,landscape painting,or related poetic theory,painting theory.Among them,the characteristics of the Buddhism of poetry and painting provide a basis on creating Zen State in garden.The ideal state of poetry constitutes the aesthetic realm of garden and its gardening techniques.

Strategies of Teaching English Poetry

This paper deals with how to teach appreciation of English poetry in college by analyzing the following items： introduction of the concept, content and writing style, the realistic meaning, multi-media aided teaching and translating English poetry into Chinese.

Beauty in Sense, Sound and Form in Poetry Translation —— Translation of Tang Poems From Chinese to English

本文对诗歌翻译的三要素 :意美、声美、形美作一简要的介绍和分析。其中主要以唐诗的翻译为例 ,说明翻译是一种再创作的过程。

Aesthetic comparison between Chinese memorial poetry and western elegy

Owing to the cultural, philosophical and religious differences, Chinese memorial poetry and western elegy show different attitudes towards death and have different ways to express it. Therefore, they have different aesthetic preference to death. This paper focuses on the basic characteristics of Chinese memorial poetry and western elegy. It analyzes the causes of these differences from the aspect of the content and style, ways of expression and cultural psychology.

Words worth’s Poetry:from Nature and beyond Nature

William Wordsworth is one of the best known English poets in literary history. As an arch romanticist, Wordsworth is generally known as a poet of Nature, because he not only describes Nature and ordinary people who live in close touch with her, but also discloses the truth about Nature. He contributes a lot to the world literature.

On Functional Discourse Analysis Applied in Translation of Chinese Tea Poetry

In this article,the author aims to analyze some English translations of ancient Chinese tea poems in a functional linguistic point of view based mainly on Halliday's functional linguistic theory.First of all,there is a general introduction of Halliday's functional linguistic theory,secondly we will look into the features of ancient Chinese tea poetry,such as the grammar,the language and the context,and then the author picks out two typical examples for detail analyzing,at last there is a brief conclusion of the whole article.

An Investigation into the Case of Multi-interpretation in Poetry Translation

In translating poems, it is very common that different people have quite different versions of the same poem. This paper therefore intends to expound upon the underlying factors from the perspective of Hermeneutics,by exploring the relationship between the textual meaning and the textual significance of a poem, as well as the relationship between the author's intention and the textual intention of a poem, aiming to explain the key element in translating poems—multi-interpretation.

The Differences Between Conventional Poetry & Imagist Poetry

As a literary movement,Imagism,a strand of modernism,is a reaction to conventional poets.It was initiated in London at the dawn of the 20th century,but short-lived,and its effects reached British and American poetry.This thesis aims to find out the difference between imagist poetry and conventional poetry.

中国古诗英译的可译性——赏读Transplanting the Seed:Poetry and Translation

在SusanBassnett的TransplantingtheSeed一文中提出诗歌翻译的可译性的基础上,简要介绍中国古诗英译的可译性和不可译性的客观存在;通过对一些古诗的英译文本进行研读,着重分析可译的客观性,并介绍了对不可译的处理方法,如风格、音韵和文化的移植。