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Reviews on Post-earthquake Reconstruction of Qiang Culture: Three-dimensional Pattern
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作者 许虹 《Journal of Landscape Research》 2011年第9期11-13,18,共4页
Through the introduction of disaster situation of Qiang Culture after Wenchuan Earthquake, the paper emphasized that carriers of Qiang Culture had been seriously damaged, the inheritance of Qiang Culture had been affe... Through the introduction of disaster situation of Qiang Culture after Wenchuan Earthquake, the paper emphasized that carriers of Qiang Culture had been seriously damaged, the inheritance of Qiang Culture had been affected, and the environment for Qiang Culture was difficult to recover. It highlighted that three-dimensional reconstruction of Qiang Culture should stress the core task and timely and effectively rescue endangered cultural heritages of Qiang Nationality from the perspectives of material and spiritual life. It had explained focuses of three-dimensional pattern construction in detail. In terms of spatial reconstruction, it should reconstruct native culture and history while material culture was constructed, and reconstruct Qiang culture highland by depending on aborigines; in terms of cluster reconstruction, it should give support to large tourism enterprises and perfect tourism chain; in terms of ecological reconstruction, it should enhance construction and demonstration of "ecological protection pilot area of Qiang culture"; in terms of development reconstruction, it should realize coordinated unity between protection and development according to classification protection, characteristic protection and key protection, so as to form the virtuous circle of post-disaster recovery protection and sustainable development. 展开更多
关键词 Qiang culture POST-EARTHQUAKE RECONSTRUCTION three-dimensional PATTERN
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Isolation and Identification of Cancer Stem Cells from Human Osteosarcom by Serum-free Three-dimensional Culture Combined with Anticancer Drugs 被引量:7
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作者 周松 李锋 +4 位作者 肖骏 熊伟 方忠 陈文坚 牛鹏彦 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2010年第1期81-84,共4页
The cancer stem cells(CSCs)from human osteosarcoma by serum-free three-dimensional culture combined with anticancer drugs were isolated and identified.The primary cells derived from human osteosarcoma were digested by... The cancer stem cells(CSCs)from human osteosarcoma by serum-free three-dimensional culture combined with anticancer drugs were isolated and identified.The primary cells derived from human osteosarcoma were digested by trypsin to prepare a single-cell suspension,and mixed homogeneously into 1.2% alginate gel.Single-cell alginate gel was cultured with serum-free DMEM/F12 medium.Epirubicin(0.8μg/mL)was added to the medium to enrich CSCs.After cultured conventionally for 7 to 10 days,most of cells suspended in ... 展开更多
关键词 three-dimensional culture serum-free culture EPIRUBICIN OSTEOSARCOMA cancer stem cells
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Chinese medicinal compound delisheng has satisfactory anti-tumor activity,and is associated with up-regulation of endostatin in human hepatocellular carcinoma cell line HepG2 in three-dimensional culture 被引量:5
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作者 Jie Cui Ke-Jun Nan Tao Tian Ya-Huan Guo Na Zhao Lin Wang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第41期5432-5439,共8页
AIM: TO investigate the multicellular resistance of human hepatocellular carcinoma HepG2 cells in three-dimensional culture to delisheng, 5-fluorouracil and adriamycin, and the possible molecular mechanisms of delish... AIM: TO investigate the multicellular resistance of human hepatocellular carcinoma HepG2 cells in three-dimensional culture to delisheng, 5-fluorouracil and adriamycin, and the possible molecular mechanisms of delisheng. METHODS: Human hepatocellular carcinoma HepG2 cells were cultured with a liquid overlay technique. After the formation of multicellular spheroids, morphology was analyzed by phase contrast microscopy, scanning electron microscopy and transmission electron microscopy. Sensitivity of HepG2 cells to delisheng, 5-fluorouracil and adriamycin was investigated by Ml-I- assay in multicelluar spheroids and monolayers. Vascular endothelial growth factor (VEGF) and endostatin expression were analyzed in multicellular spheroids treated with delisheng, 5-fluorouracil, adriamycin and negative control PBS, with immunohistochemical staining. RESULTS: Multicellular spheroids exhibited structural characteristics somewhat different to those in monolayers. The cells in three-dimensional cell culture turned out to be less sensitive to delisheng, 5-fluorouracil and adriamycin than the cells cultured in monolayer. This showed that delisheng had a satisfactory cells inhibition ratio compared to 5-fluorouracil and adriamycin. Immunohistochemical staining showed that VEGF and endostatin expression was positive during growth as multicellular spheroids, and endostatin expression in spheroids with treatment of delisheng was higher than that with 5-fluorouracil, adriamycin and PBS (139.35 ± 7.83, 159.23 ± 10.34, 162.83 ± 3.47 and 148.48 ± 11.06, P 〈 0.05).CONCLUSION: Chinese medicine compound delisheng has satisfactory anti-tumor activity in HepG2 cells in three-dimensional culture, and the effects are associated with up-regulation of endostatin. 展开更多
关键词 Delisheng GINSENG three-dimensional culture Multicellular resistance ENDOSTATIN
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Three-dimensional cell culture systems as an in vitro platform for cancer and stem cell modeling 被引量:11
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作者 Nipha Chaicharoenaudomrung Phongsakorn Kunhorm Parinya Noisa 《World Journal of Stem Cells》 SCIE 2019年第12期1065-1083,共19页
Three-dimensional(3D)culture systems are becoming increasingly popular due to their ability to mimic tissue-like structures more effectively than the monolayer cultures.In cancer and stem cell research,the natural cel... Three-dimensional(3D)culture systems are becoming increasingly popular due to their ability to mimic tissue-like structures more effectively than the monolayer cultures.In cancer and stem cell research,the natural cell characteristics and architectures are closely mimicked by the 3D cell models.Thus,the 3D cell cultures are promising and suitable systems for various proposes,ranging from disease modeling to drug target identification as well as potential therapeutic substances that may transform our lives.This review provides a comprehensive compendium of recent advancements in culturing cells,in particular cancer and stem cells,using 3D culture techniques.The major approaches highlighted here include cell spheroids,hydrogel embedding,bioreactors,scaffolds,and bioprinting.In addition,the progress of employing 3D cell culture systems as a platform for cancer and stem cell research was addressed,and the prominent studies of 3D cell culture systems were discussed. 展开更多
关键词 three-dimensional cultureS CANCER Stem cells Disease MODELING In VITRO screening PLATFORM
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Simplified three-dimensional culture system for long-term expansion of embryonic stem cells 被引量:2
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作者 Christina McKee Mick Perez-Cruet +1 位作者 Ferman Chavez G Rasul Chaudhry 《World Journal of Stem Cells》 SCIE CAS 2015年第7期1064-1077,共14页
AIM: To devise a simplified and efficient method for long-term culture and maintenance of embryonic stem cells requiring less frequent passaging. METHODS: Mouse embryonic stem cells(ESCs) labeled with enhanced yellow ... AIM: To devise a simplified and efficient method for long-term culture and maintenance of embryonic stem cells requiring less frequent passaging. METHODS: Mouse embryonic stem cells(ESCs) labeled with enhanced yellow fluorescent protein were cultured in three-dimensional(3-D) self-assembling scaffolds and compared with traditional two-dimentional(2-D) culture techniques requiring mouse embryonic fibroblast feeder layers or leukemia inhibitory factor. 3-D scaffolds encapsulating ESCs were prepared by mixing ESCs with polyethylene glycol tetra-acrylate(PEG-4-Acr) and thiolfunctionalized dextran(Dex-SH). Distribution of ESCs in 3-D was monitored by confocal microscopy. Viability and proliferation of encapsulated cells during long-term culture were determined by propidium iodide as well as direct cell counts and PrestoB lue(PB) assays. Genetic expression of pluripotency markers(Oct4, Nanog, Klf4, and Sox2) in ESCs grown under 2-D and 3-D cultureconditions was examined by quantitative real-time polymerase chain reaction. Protein expression of selected stemness markers was determined by two different methods, immunofluorescence staining(Oct4 and Nanog) and western blot analysis(Oct4, Nanog, and Klf4). Pluripotency of 3-D scaffold grown ESCs was analyzed by in vivo teratoma assay and in vitro differentiation via embryoid bodies into cells of all three germ layers. RESULTS: Self-assembling scaffolds encapsulating ESCs for 3-D culture without the loss of cell viability were prepared by mixing PEG-4-Acr and Dex-SH(1:1 v/v) to a final concentration of 5%(w/v). Scaffold integrity was dependent on the degree of thiol substitution of Dex-SH and cell concentration. Scaffolds prepared using Dex-SH with 7.5% and 33% thiol substitution and incubated in culture medium maintained their integrity for 11 and 13 d without cells and 22 ± 5 d and 37 ± 5 d with cells, respectively. ESCs formed compact colonies, which progressively increased in size over time due to cell proliferation as determined by confocal microscopy and PB staining. 3-D scaffold cultured ESCs expressed significantly higher levels(P < 0.01) of Oct4, Nanog, and Kl4, showing a 2.8, 3.0 and 1.8 fold increase, respectively, in comparison to 2-D grown cells. A similar increase in the protein expression levels of Oct4, Nanog, and Klf4 was observed in 3-D grown ESCs. However, when 3-D cultured ESCs were subsequently passaged in 2-D culture conditions, the level of these pluripotent markers was reduced to normal levels. 3-D grown ESCs produced teratomas and yielded cells of all three germ layers, expressing brachyury(mesoderm), NCAM(ectoderm), and GATA4(endoderm) markers. Furthermore, these cells differentiated into osteogenic, chondrogenic, myogenic, and neural lineages expressing Col1, Col2, Myog, and Nestin, respectively. CONCLUSION: This novel 3-D culture system demonstrated long-term maintenance of mouse ESCs without the routine passaging and manipulation necessary for traditional 2-D cell propagation. 展开更多
关键词 three-dimensional culture PLURIPOTENCY EMBRYONIC s
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Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis 被引量:4
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作者 Jeong Ah Kim Soohyun Hong Won Jong Rhee 《World Journal of Stem Cells》 SCIE 2019年第10期803-816,共14页
Although the recent advances in stem cell engineering have gained a great deal of attention due to their high potential in clinical research,the applicability of stem cells for preclinical screening in the drug discov... Although the recent advances in stem cell engineering have gained a great deal of attention due to their high potential in clinical research,the applicability of stem cells for preclinical screening in the drug discovery process is still challenging due to difficulties in controlling the stem cell microenvironment and the limited availability of high-throughput systems.Recently,researchers have been actively developing and evaluating three-dimensional(3D)cell culture-based platforms using microfluidic technologies,such as organ-on-a-chip and organoid-on-a-chip platforms,and they have achieved promising breakthroughs in stem cell engineering.In this review,we start with a comprehensive discussion on the importance of microfluidic 3D cell culture techniques in stem cell research and their technical strategies in the field of drug discovery.In a subsequent section,we discuss microfluidic 3D cell culture techniques for high-throughput analysis for use in stem cell research.In addition,some potential and practical applications of organ-on-a-chip or organoid-on-a-chip platforms using stem cells as drug screening and disease models are highlighted. 展开更多
关键词 STEM CELL Microfluidic TECHNOLOGY three-dimensional CELL culture Highthroughput SCREENING
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Optimization of Three-Dimensional Culture Conditions of HepG2 Cells with Response Surface Methodology Based on the VitroGel System
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作者 WANG Jing Bo QIN Wen +7 位作者 YANG Zhuo SHEN Shi MA Yan WANG Li Yuan ZHUO Qin GONG Zhao Long HUO Jun Sheng CHEN Chen 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2022年第8期688-698,共11页
Objective This study optimizes three-dimensional(3D) culture conditions of HepG2 using response surface methodology(RSM) based on the VitroGel system to facilitate the cell model in vitro for liver tissues.Method HepG... Objective This study optimizes three-dimensional(3D) culture conditions of HepG2 using response surface methodology(RSM) based on the VitroGel system to facilitate the cell model in vitro for liver tissues.Method HepG2 cell was 3D cultured on the VitroGel system.Cell viability was detected using Cell Counting Kit-8(CCK-8) assay of HepG2 lived cell numbers.The proliferation of HepG2 cell and clustering performance was measured via fluorescence staining test.Albumin concentration in the culture medium supernatant as an index of HepG2 cell biological function was measured with ELISA kit.Independent factor tests were conducted with three key factors:inoculated cell concentration,cultured time,and dilution degree of the hydrogel.The preliminary results of independent factor tests were used to determine the levels of factors for RSM.Result The selected optimal culture conditions are as follows:concentration of inoculated cells was4.44 × 10^(5)/mL,culture time was 4.86 days,and hydrogel dilution degree was 1:2.23.The result shows that under optimal conditions,the predicted optical density(OD) value of cell viability was 3.10 and measured 2.978 with a relative error of 3.94%.Conclusion This study serves as a reference for the 3D HepG2 culture and constructs liver tissues in vitro.Additionally,it provides the foundation for repeated dose high-throughput toxicity studies and other scientific research work. 展开更多
关键词 Response surface methodology The three-dimensional culture HYDROGEL
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Irradiation Response of Adipose-derived Stem Cells under Three-dimensional Culture Condition
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作者 DU Ya Rong PAN Dong +5 位作者 CHEN Ya Xiong XUE Gang REN Zhen Xin LI Xiao Man ZHANG Shi Chuan HU Bu Rong 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2015年第8期549-557,共9页
Objective Adipose tissue distributes widely in human body. The irradiation response of the adipose cells in vivo remains to be investigated. In this study we investigated irradiation response of adipose-derived stem c... Objective Adipose tissue distributes widely in human body. The irradiation response of the adipose cells in vivo remains to be investigated. In this study we investigated irradiation response of adipose-derived stem cells (ASCs) under three-dimensional culture condition. Methods ASCs were isolated and cultured in low attachment dishes to form three-dimensional (3D) spheres in vitro. The neuronal differentiation potential and stem-liked characteristics was monitored by using immunofluoresence staining and flow cytometry in monolayer and 3D culture. To investigate the irradiation sensitivity of 3D sphere culture, the fraction of colony survival and micronucleus were detected in monolayer and 3D culture. Soft agar assays were performed for measuring malignant transformation for the irradiated monolayer and 3D culture. Results The 3D cultured ASCs had higher differentiation potential and an higher stem-like cell percentage. The 3D cultures were more radioresistant after either high linear energy transfer (LET) carbon ion beam or low LET X-ray irradiation compared with the monolayer cell. The ASCs’ potential of cellular transformation was lower after irradiation by soft agar assay. Conclusion These findings suggest that adipose tissue cell are relatively genomic stable and resistant to genotoxic stress. 展开更多
关键词 Adipose-derived stem cells three-dimensional cell culture Irradiation response High-and low-LET irradiation
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Inhibition of mammalian target of rapamycin induces phenotypic reversion in three-dimensional cultures of malignant breast epithelial cells
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作者 Ross Booth Soonjo Kwon Eric Monson 《Journal of Biomedical Science and Engineering》 2010年第5期476-483,共8页
Inhibition of mammalian target of rapamycin (m- TOR) is a potential method for cancer treatment. Effects of rapamycin (RAP) on the reversion of malignant breast epithelial cells were investigated on three-dimensional ... Inhibition of mammalian target of rapamycin (m- TOR) is a potential method for cancer treatment. Effects of rapamycin (RAP) on the reversion of malignant breast epithelial cells were investigated on three-dimensional (3D) basement membrane extract (BME) cultures. Through continuous exposure to 20 nM of RAP, cell colony size was significantly reduced in 3D BME cultures of malignant breast epithelial cells, while normal cell colony size appeared unaffected. In unfixed 3D BME cultures of normal and RAP-treated malignant breast epithelial cells, the presence of luminal cell death was confirmed by ethidium bromide and propidium iodide labeling. Increased structural organization was observed by im- munofluorescence staining of F-actin and β-catenin in RAP-treated malignant breast epithelial cells. In monolayer cultures of normal and malignant breast epithelial cells, continuous exposure to 20 nM of RAP increased caspase 3/7 activity and decreased proliferation. Reverse transcriptase polymerase ch- ain reaction (RT-PCR) array analysis indicated a fold increase in the expression of a number of proteins related to polarity, cell-cell adhesion, and cell-matrix adhesion in the presence of RAP. Our data showed that phenotypic reversion of malignancy can be ach- ieved through RAP exposure on 3D BME cultures. This 3D BME culture system will provide correct microenvironments for observing the effects of other mTOR inhibitors on phenotypic reversion of malignant breast epithelial cells. 展开更多
关键词 RAPAMYCIN three-dimensional culture BREAST Cancer REVERSION BASEMENT Membrane Extract mTOR Inhibitors
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Training image analysis for three-dimensional reconstruction of porous media
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作者 滕奇志 杨丹 +2 位作者 徐智 李征骥 何小海 《Journal of Southeast University(English Edition)》 EI CAS 2012年第4期415-421,共7页
In order to obtain a better sandstone three-dimensional (3D) reconstruction result which is more similar to the original sample, an algorithm based on stationarity for a two-dimensional (2D) training image is prop... In order to obtain a better sandstone three-dimensional (3D) reconstruction result which is more similar to the original sample, an algorithm based on stationarity for a two-dimensional (2D) training image is proposed. The second-order statistics based on texture features are analyzed to evaluate the scale stationarity of the training image. The multiple-point statistics of the training image are applied to obtain the multiple-point statistics stationarity estimation by the multi-point density function. The results show that the reconstructed 3D structures are closer to reality when the training image has better scale stationarity and multiple-point statistics stationarity by the indications of local percolation probability and two-point probability. Moreover, training images with higher multiple-point statistics stationarity and lower scale stationarity are likely to obtain closer results to the real 3D structure, and vice versa. Thus, stationarity analysis of the training image has far-reaching significance in choosing a better 2D thin section image for the 3D reconstruction of porous media. Especially, high-order statistics perform better than low-order statistics. 展开更多
关键词 three-dimensional reconstruction training image stationarity porous media multiple-point statistics
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American Multinational Media and the Expansion of American Mass Culture
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作者 王茜 《海外英语》 2011年第12X期271-272,共2页
With the development of globalization and gradual intensification of mutual dependence of the world, "hard power" which includes economic and military strength is no longer the primary consideration for many... With the development of globalization and gradual intensification of mutual dependence of the world, "hard power" which includes economic and military strength is no longer the primary consideration for many countries. They tend to pay more attention to the attractiveness of their "soft power",which includes values,life style , ideology and so forth. Just as Joseph S. Ny , the famous professor of Harvard who puts forward the concept of soft power, says ,"only by widespread communication and diffusion can a country reinforces its soft power". This is mass media's function. Since the 1990s, tens of thousands cultural products brought in from America are exported to foreign countries, including popular music, films ,TV programs, magazines, books and so forth. By making advantage of its monopoly position in media circle, the United States pursues cultural hegemonism and expands its popular culture all over the world. This thesis takes a general view of the great advantages of America multi-national media groups and how they push forward the American popular culture all over the world. 展开更多
关键词 culturAL HEGEMONISM multi-national media soft power
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Media Convergence:the Culture Dimensions of Thinking 被引量:1
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作者 shen diao lan ju 《学术界》 CSSCI 北大核心 2017年第11期314-323,共10页
In the process of meida Convergence,many researchers paid excessive attention to media technology, industry and management,and ignored the culture dimensions of media convergence. Therefore,to transcend media converge... In the process of meida Convergence,many researchers paid excessive attention to media technology, industry and management,and ignored the culture dimensions of media convergence. Therefore,to transcend media convergence technology, industrial thinking and more to the particularity attach importance to cultural media, it is a right way to achieve media convergence. But in the context of China's culture,media convergence should value the cultural uniqueness and the imbalance of the realistic problems,to reach innovation and breakthrough. 展开更多
关键词 media CONVERGENCE culturAL THINKING CONVERGENCE culture Chinese CONTEXT
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Are Early Embryo Cleavage Kinetics Affected by Energy Substrates in Different Culture Media? 被引量:1
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作者 Bing-Xin MA Bo HUANG +2 位作者 Dan CHEN Lei JIN Qun RAO 《Current Medical Science》 SCIE CAS 2022年第6期1297-1304,共8页
Objective This study aimed to investigate the influence of different culture media on early embryonic cleavage kinetics using time-lapse analysis and to determine the possible relationships between energy substrates i... Objective This study aimed to investigate the influence of different culture media on early embryonic cleavage kinetics using time-lapse analysis and to determine the possible relationships between energy substrates in culture media and the cleavage kinetics.Methods A total of 10021 embryos from 1310 couples were cultured in time-lapse incubators.Embryos cultured in Vitrolife media were allocated to group I,and those in COOK media to group II.Embryo cleavage time points up to the 8-cell stage(t2–t8)were observed after pronuclei fading.Results The baseline demographic features,in vitro fertilization indications,ovarian stimulation protocol,oocyte-cumulus complexes,fertilization rate,together with pregnancy and perinatal outcomes were similar(P>0.05)between groups I and II.According to the time-lapse analysis,all embryos in group I showed significantly faster cleavage speed than those in group II(P<0.05).Furthermore,there was better synchrony in division(s3)and a longer length of the third cell cycle duration(cc3)in group II.Interestingly,implanted embryos in group II showed faster cleavage speed than those in group I,especially at t4 and t7.The glucose contents and multiple major amino acids were similar between the two groups.Lactic and pyruvic acid contents were generally higher in group I than those in group II.Conclusion Because different commercial culture media may influence cleavage kinetics of embryos,it is essential for embryologists to take culture media into consideration in selecting a potential embryo when using a time-lapse system before implantation. 展开更多
关键词 embryo culture TIME-LAPSE cleavage kinetics culture media energy substrate
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A Sensitive Competitive ELISA for Determination of Biotin in Transformed Yeast Culture Media 被引量:1
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作者 YANGHong 《Journal of Chinese Pharmaceutical Sciences》 CAS 2003年第4期201-206,共6页
Aim To develop a sensitive competitive ELBA for the determination of biotinin transformed yeast culture media. Methods The ELBA plate was firstly coated with Mycoplasmahyopneumoniae, and then successively incubated wi... Aim To develop a sensitive competitive ELBA for the determination of biotinin transformed yeast culture media. Methods The ELBA plate was firstly coated with Mycoplasmahyopneumoniae, and then successively incubated with rabbit anti-Mycoplasma hyopneumoniae serum andgoat anti-rabbit IgG-biotin to form the solid biotin, which competed with the biotin in the solution(standard or sample) for the limited streptavidin-horse radish peroxidase conjugate. The standardcalibration curve for biotin analysis was constructed in the range of 50 - 2000 ng·L^(-1). ResultsThe detection limit for biotin was found to be 83 ng·L^(-1), which was about 1000 times lower thanthe lowest determination concentration in the reported ELISA for biotin analysis. The relativestandard deviations for the spiked samples at biotin concentrations of 200 ng·L^(-1), 500ng·L^(-1), and 1000 ng·L^(-1) were 24.87%, 6.15% , and 7.86% , respectively, with the averagerecovery of 101.13% . The wild yeast and its sixty-three transformed yeast culture media wereapplied to the developed ELBA for the determination of biotin. It was found that the biotinconcentrations in more than 85% of the tested samples were enhanced with different increase factorsafter transformation. Conclusion Utilization of Mycoplasma hyopneumoniae as the coating proteinimproves the precision and accuracy of the ELBA assay, which might be used for the biotin assay inother media. 展开更多
关键词 affinity assay biotin analysis STREPTAVIDIN ELISA coating transformedyeast culture media mycoplasma hyopneumoniae
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Teaching Path and Countermeasures of New Media Advertising Based on “Three-dimensional” Practical Teaching System 被引量:1
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作者 WANG Hui CHEN Chunxiao LI Xiu 《Journal of Landscape Research》 2022年第6期95-98,共4页
By analyzing the dilemma encountered in practical teaching of new media advertising course in the digital economy era, this paper explores the teaching path of new media advertising course based on “three-dimensional... By analyzing the dilemma encountered in practical teaching of new media advertising course in the digital economy era, this paper explores the teaching path of new media advertising course based on “three-dimensional” practical teaching system, and believes that it is necessary to accurately determine talent training objectives, and construct the three dimensions of practical teaching, practical ability, and new media entrepreneurship and employment. Finally, the teaching countermeasures of practical teaching system based on three dimensions are put forward. 展开更多
关键词 three-dimensional”practical teaching system New media advertising PATH COUNTERMEASURE
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Modulation of the Nogo signaling pathway to overcome amyloid-β-mediated neurite inhibition in human pluripotent stem cell-derived neurites
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作者 Kirsty Goncalves Stefan Przyborski 《Neural Regeneration Research》 SCIE CAS 2025年第9期2645-2654,共10页
Neuronal cell death and the loss of connectivity are two of the primary pathological mechanisms underlying Alzheimer's disease.The accumulation of amyloid-βpeptides,a key hallmark of Alzheimer's disease,is be... Neuronal cell death and the loss of connectivity are two of the primary pathological mechanisms underlying Alzheimer's disease.The accumulation of amyloid-βpeptides,a key hallmark of Alzheimer's disease,is believed to induce neuritic abnormalities,including reduced growth,extension,and abnormal growth cone morphology,all of which contribute to decreased connectivity.However,the precise cellular and molecular mechanisms governing this response remain unknown.In this study,we used an innovative approach to demonstrate the effect of amyloid-βon neurite dynamics in both two-dimensional and three-dimensional cultu re systems,in order to provide more physiologically relevant culture geometry.We utilized various methodologies,including the addition of exogenous amyloid-βpeptides to the culture medium,growth substrate coating,and the utilization of human-induced pluripotent stem cell technology,to investigate the effect of endogenous amyloid-βsecretion on neurite outgrowth,thus paving the way for potential future applications in personalized medicine.Additionally,we also explore the involvement of the Nogo signaling cascade in amyloid-β-induced neurite inhibition.We demonstrate that inhibition of downstream ROCK and RhoA components of the Nogo signaling pathway,achieved through modulation with Y-27632(a ROCK inhibitor)and Ibuprofen(a Rho A inhibitor),respectively,can restore and even enhance neuronal connectivity in the presence of amyloid-β.In summary,this study not only presents a novel culture approach that offers insights into the biological process of neurite growth and inhibition,but also proposes a specific mechanism for reduced neural connectivity in the presence of amyloid-βpeptides,along with potential intervention points to restore neurite growth.Thereby,we aim to establish a culture system that has the potential to serve as an assay for measuring preclinical,predictive outcomes of drugs and their ability to promote neurite outgrowth,both generally and in a patient-specific manner. 展开更多
关键词 Alzheimer's disease induced pluripotent stem cell neurite outgrowth neuron NOGO Rho A ROCK stem cell three-dimensional culture
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A Study on Rural Cultural Communication of Short Videos From the Perspective of Mediation-Taking the Popularization of “Village BA” (Village Basketball Association) in Qiandongnan as an Example
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作者 SHEN Lei JIA Peng WEI Xiaojia 《Journalism and Mass Communication》 2024年第3期209-214,共6页
With the rise of new media and short videos shaping the new communication environment,rural culture has been mediated and transformed to spread to a wider region.As a cultural achievement of rural revitalization,“Vil... With the rise of new media and short videos shaping the new communication environment,rural culture has been mediated and transformed to spread to a wider region.As a cultural achievement of rural revitalization,“Village BA”(Village Basketball Association)demonstrates Chinese modernization.The use of short videos,mass rural movements,and“spectacle”spaces to attract spectators has become a key issue in the dissemination of rural culture. 展开更多
关键词 new media mediated rural culture short video “Village BA”
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3D Collagen Gels:A Promising Platform for Dendritic Cell Culture in Biomaterials Research
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作者 Kirubanandan Shanmugam 《Proceedings of Anticancer Research》 2024年第4期124-134,共11页
The three-dimensional(3D)cell culture system has garnered significant attention in recent years as a means of studying cell behavior and tissue development,as opposed to traditional two-dimensional cultures.These syst... The three-dimensional(3D)cell culture system has garnered significant attention in recent years as a means of studying cell behavior and tissue development,as opposed to traditional two-dimensional cultures.These systems can induce specific cell reactions,promote specific tissue functions,and serve as valuable tools for research in tissue engineering,regenerative medicine,and drug discovery.This paper discusses current developments in the field of three-dimensional cell culture and the potential applications of 3D type 1 collagen gels to enhance the growth and maturation of dendritic cells. 展开更多
关键词 three-dimensional cell culture Dendritic cells Type 1 collagen gels Bovine tendons and rat tails
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Coscinium fenestratum: Callus and Suspension Cell Culture of the Endangered Medicinal Plant Using Vermicompost Extract and Coelomic Fluid as Plant Tissue Culture Media
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作者 Suman Kashyap Neera Kapoor Radha D. Kale 《American Journal of Plant Sciences》 2016年第6期899-906,共8页
In vitro tissue culture of hard woody, endangered, medicinal plant Coscinium fenestratum is most challenging to plant tissue culturists. In the present study, petiole and leaf explants of Coscinium fenestratum were in... In vitro tissue culture of hard woody, endangered, medicinal plant Coscinium fenestratum is most challenging to plant tissue culturists. In the present study, petiole and leaf explants of Coscinium fenestratum were induced to form callus when cultured on vermicompost extract media along with coelomic fluid. Suspension medium was developed using vermicompost extract and coelomic fluid in 3:1 ratio. Phytochemical analysis of the alkaloid berberine was confirmed from callus, suspension cell culture and suspension medium by Thin Layer Chromatography and High Performance Liquid Chromatography. Vermicompost and its extracts with coelomic fluid have shown maximum (100 per cent) response of callus induction. Callus mass enlarged with increasing concentration of coelomic fluid and callus growth was assessed from the biomass. Incubation of culture tubes in dark supported callus development significantly. The Rf value of 0.36 confirmed the presence of berberine by Thin Layer Chromatography. Qualitative analysis confirmed the presence of alkaloid berberine with the retention time of 2.8 minutes similar to that of standard reference sample from Sigma chemicals, USA. The suspension medium turned deep yellow because of the release of the alkaloid. Vermicompost and its extracts along with coelomic fluid have shown the economical approach for micropropagation of economically and medicinally important plants. 展开更多
关键词 BERBERINE Coelomic Fluid Coscinium fenestratum Plant Tissue culture media Suspension Cell culture Vermicompost Extract
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Analysis of Communication Model of Chinese Traditional Culture in the New Media Era
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作者 Xing Nian Liu Hao 《学术界》 CSSCI 北大核心 2020年第5期201-209,共9页
Nowadays,the communication of traditional culture is facing various challenges,including limitations of traditional culture itself,decrease of inheritors,poor construction of structure,and heterization of connotation,... Nowadays,the communication of traditional culture is facing various challenges,including limitations of traditional culture itself,decrease of inheritors,poor construction of structure,and heterization of connotation,as well as conflicts between Chinese and foreign cultures in the context of globalization.New media has provided more opportunities for the communication of outstanding Chinese traditional culture.This paper analyzed the role of new media in completing the structure and expanding the scope of communication of outstanding Chinese traditional culture,and proposed strategy for optimizing the communication route from the perspective of improving environment,changing content,and adjusting strategy.The contributions of new media to the communication of Chinese traditional culture should be highly appreciated. 展开更多
关键词 new media Chinese traditional culture communication model
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