BACKGROUND： It is not possible to reconstruct the inner structure of the spinal cord, such as gray matter and spinal tracts, from the Visual Human Project database or CT and MRI databases, due to low image resolution...BACKGROUND： It is not possible to reconstruct the inner structure of the spinal cord, such as gray matter and spinal tracts, from the Visual Human Project database or CT and MRI databases, due to low image resolution and contrast in macrosection images. OBJECTIVE： To explore a semi-automatic computerized three-dimensional （3D） reconstruction of human spinal cord based on histological serial sections, in order to solve issues such as low contrast. DESIGN, TIME AND SETTING： An experimental study combining serial section techniques and 3D reconstruction, performed in the laboratory of Human Anatomy and Histoembryology at the Medical School of Nantong University during January to April 2008. SETTING： Department of Anatomy, Institute of Neurobiology, Jiangsu Province Key Laboratory of Neural Regeneration, Laboratory of Image Engineering. MATERIALS： A human lumbar spinal cord segment from fresh autopsy material of an adult male. METHODS： After 4% paraformaldehyde fixation for three days, serial sections of the lumbar spinal cord were cut on a Leica cryostat and mounted on slides in sequence, with eight sections aligned separately on each slide. All sections were stained with Luxol Fast Blue to reveal myelin sheaths. After gradient dehydration and clearing, the stained slides were coverslipped. Sections were observed and images recorded under a light microscope using a digital camera. Six images were acquired at x25 magnification and automatically stitched into a complete section image. After all serial images were obtained, 96 complete serial images of the human lumbar cord segment were automatically processed with ＂Curves＂, ＂Autocontrast＂, ＂Gray scale 8 bit＂, ＂Invert＂, ＂Image resize to 50%＂ steps using Photoshop 7.0 software. All images were added in order into 3D-DOCTOR 4.0 software as a stack, where serial images were automatically realigned with neighboring images and semi-automatically segmented for white matter and gray matter. Finally, simple surface and volume reconstruction were completed on a personal computer. The reconstructed human lumbar spinal cord segment was interactively observed, cut, and measured. MAIN OUTCOME MEASURES： The reconstructed human lumbar spinal cord segment. RESULTS： Compared with serial images obtained from other image modalities, such as CT, MRI, and macrosections from The Visual Human Project database, the Luxol Fast Blue stained histological serial section images exhibited higher resolution and contrast between gray and white matter. Image processing and 3D reconstruction steps were semi-automatically performed with related software. The 3D reconstructed human lumbar cord segment were observed, cut, and measured on a PC. CONCLUSION： A semi-automatically computerized method, based on histological serial sections, is an effective way to 3D-reconstruct the human spinal cord.展开更多
Wire and arc additive manufacturing(WAAM) shows a great promise for fabricating fully dense metal parts by means of melting materials in layers using a welding heat source. However, due to a large layer height produce...Wire and arc additive manufacturing(WAAM) shows a great promise for fabricating fully dense metal parts by means of melting materials in layers using a welding heat source. However, due to a large layer height produced in WAAM, an unsatisfactory surface roughness of parts processed by this technology has been a key issue. A methodology based on laser vision sensing is proposed to quantitatively calculate the surface roughness of parts deposited by WAAM.Calibrations for a camera and a laser plane of the optical system are presented. The reconstruction precision of the laser vision system is verified by a standard workpiece. Additionally, this determination approach is utilized to calculate the surface roughness of a multi-layer single-pass thin-walled part. The results indicate that the optical measurement approach based on the laser vision sensing is a simple and effective way to characterize the surface roughness of parts deposited by WAAM. The maximum absolute error is less than 0.15 mm. The proposed research provides the foundation for surface roughness optimization with different process parameters.展开更多
Residual stress during the machining process has always been a research hotspot,especially for aero-engine blades.The three-dimensional modeling and reconstructive laws of residual stress among various processes in th...Residual stress during the machining process has always been a research hotspot,especially for aero-engine blades.The three-dimensional modeling and reconstructive laws of residual stress among various processes in the machining process of the fan blade is studied in this paper.The fan blades of Ti-6Al-4V are targeted for milling,polishing,heat treatment,vibratory finishing,and shot peening.The surface and subsurface residual stress after each process is measured by the X-ray diffraction method.The distribution of the surface and subsurface residual stress is analyzed.The Rational Taylor surface function and cosine decay function are used to fit the characteristic function of the residual stress distribution,and the empirical formula with high fitting accuracy is obtained.The value and distribution of surface and subsurface residual stress vary greatly due to different processing techniques.The reconstructive change of the surface and subsurface residual stress of the blade in each process intuitively shows the change of the residual stress between the processes,which has a high reference significance for the research on the residual stress of the blade processing and the optimization of the entire blade process.展开更多
基金Natural Science Research Plan forJiangsu Colleges, No.05KJB180105 Postgraduate Innovation Cultivating Projectin Jiangsu Province, No.CX07s_035z
文摘BACKGROUND： It is not possible to reconstruct the inner structure of the spinal cord, such as gray matter and spinal tracts, from the Visual Human Project database or CT and MRI databases, due to low image resolution and contrast in macrosection images. OBJECTIVE： To explore a semi-automatic computerized three-dimensional （3D） reconstruction of human spinal cord based on histological serial sections, in order to solve issues such as low contrast. DESIGN, TIME AND SETTING： An experimental study combining serial section techniques and 3D reconstruction, performed in the laboratory of Human Anatomy and Histoembryology at the Medical School of Nantong University during January to April 2008. SETTING： Department of Anatomy, Institute of Neurobiology, Jiangsu Province Key Laboratory of Neural Regeneration, Laboratory of Image Engineering. MATERIALS： A human lumbar spinal cord segment from fresh autopsy material of an adult male. METHODS： After 4% paraformaldehyde fixation for three days, serial sections of the lumbar spinal cord were cut on a Leica cryostat and mounted on slides in sequence, with eight sections aligned separately on each slide. All sections were stained with Luxol Fast Blue to reveal myelin sheaths. After gradient dehydration and clearing, the stained slides were coverslipped. Sections were observed and images recorded under a light microscope using a digital camera. Six images were acquired at x25 magnification and automatically stitched into a complete section image. After all serial images were obtained, 96 complete serial images of the human lumbar cord segment were automatically processed with ＂Curves＂, ＂Autocontrast＂, ＂Gray scale 8 bit＂, ＂Invert＂, ＂Image resize to 50%＂ steps using Photoshop 7.0 software. All images were added in order into 3D-DOCTOR 4.0 software as a stack, where serial images were automatically realigned with neighboring images and semi-automatically segmented for white matter and gray matter. Finally, simple surface and volume reconstruction were completed on a personal computer. The reconstructed human lumbar spinal cord segment was interactively observed, cut, and measured. MAIN OUTCOME MEASURES： The reconstructed human lumbar spinal cord segment. RESULTS： Compared with serial images obtained from other image modalities, such as CT, MRI, and macrosections from The Visual Human Project database, the Luxol Fast Blue stained histological serial section images exhibited higher resolution and contrast between gray and white matter. Image processing and 3D reconstruction steps were semi-automatically performed with related software. The 3D reconstructed human lumbar cord segment were observed, cut, and measured on a PC. CONCLUSION： A semi-automatically computerized method, based on histological serial sections, is an effective way to 3D-reconstruct the human spinal cord.
基金Supported by National Natural Science Foundation of China(Grant Nos.51505394,61573293)Key Technologies R&D Program of Sichuan Province of China(Grant No.2015GZ0305)
文摘Wire and arc additive manufacturing(WAAM) shows a great promise for fabricating fully dense metal parts by means of melting materials in layers using a welding heat source. However, due to a large layer height produced in WAAM, an unsatisfactory surface roughness of parts processed by this technology has been a key issue. A methodology based on laser vision sensing is proposed to quantitatively calculate the surface roughness of parts deposited by WAAM.Calibrations for a camera and a laser plane of the optical system are presented. The reconstruction precision of the laser vision system is verified by a standard workpiece. Additionally, this determination approach is utilized to calculate the surface roughness of a multi-layer single-pass thin-walled part. The results indicate that the optical measurement approach based on the laser vision sensing is a simple and effective way to characterize the surface roughness of parts deposited by WAAM. The maximum absolute error is less than 0.15 mm. The proposed research provides the foundation for surface roughness optimization with different process parameters.
基金This work was funded by the National Natural Science Foundation of China(Grant Nos.51875472,91860206,and 51905440)the National Science and Technology Major Project(Grant No.2017-VII-0001-0094)+1 种基金the National Key Research and Development Plan in Shaanxi Province of China(Grant No.2019ZDLGY02-03)the Natural Science Basic Research Plan in Shaanxi Province of China(Grant No.2020JQ-186).
文摘Residual stress during the machining process has always been a research hotspot,especially for aero-engine blades.The three-dimensional modeling and reconstructive laws of residual stress among various processes in the machining process of the fan blade is studied in this paper.The fan blades of Ti-6Al-4V are targeted for milling,polishing,heat treatment,vibratory finishing,and shot peening.The surface and subsurface residual stress after each process is measured by the X-ray diffraction method.The distribution of the surface and subsurface residual stress is analyzed.The Rational Taylor surface function and cosine decay function are used to fit the characteristic function of the residual stress distribution,and the empirical formula with high fitting accuracy is obtained.The value and distribution of surface and subsurface residual stress vary greatly due to different processing techniques.The reconstructive change of the surface and subsurface residual stress of the blade in each process intuitively shows the change of the residual stress between the processes,which has a high reference significance for the research on the residual stress of the blade processing and the optimization of the entire blade process.
