Pseudomonas aeruginosa(P:aeruginosa)is a major opportunistic pathogen in hospital-acquired infections.Thus,carly diagnosis is the best strategy for fighting against these infections.In this report,we incorporated mult...Pseudomonas aeruginosa(P:aeruginosa)is a major opportunistic pathogen in hospital-acquired infections.Thus,carly diagnosis is the best strategy for fighting against these infections.In this report,we incorporated multiple crOsS displacement amplification(MCDA)combined with the malachite green(MG)for rapid,sensitive,specific and visual detection of P.aeruginosa by targeting the oprl gene.The MCDA-MG assay was conducted at 67°C for only 40 min during the amplification stage,and then products were directly detected by using colorimetric indicators(MG),eliminating the use of an electrophoresis instrument or amplicon analysis equipment.The entire process,including specimen processing(35 min),amplification(40 min)and detection(5 min),can be finished within 80 min.All 30 non-P.aeruginosa strains tested negative,indicating the high specificity of the MCDA primers.The analytical sensitivity of the MCDA-MG assay was 100 fg of genomic templates per reaction in pure culture,which was in complete accordance with MCDA by gel clectrophoresis and real-time turbidity.The assay was also successfully applied to detecting P.aeruginosa in stool samples.Therefore,the rapidity,simplicity,and nearly equipment free platform of the MCDA-MG technique make it possible for clinical diagnosis,and more.展开更多
In large mines,single fan is usually not enough to ventilate all the working areas.Single mine-fan approach cannot be directly applied to multiple-fan networks because the present of multiple pressures and air quantit...In large mines,single fan is usually not enough to ventilate all the working areas.Single mine-fan approach cannot be directly applied to multiple-fan networks because the present of multiple pressures and air quantities associated with each fan in the network.Accordingly,each fan in a multiple-fan system has its own mine characteristic curve,or a subsystem curve.Under some consideration,the conventional concept of a mine characteristic curve of a single-fan system can be directly extended to that of a particular fan within a multiple-fan system.In this paper the mutual effect of the fans on each other and their effect on the stability of the ventilation network were investigated by Hardy Cross algorithm combined with a switching-parameters technique.To show the validity and reliability of this algorithm,the stability of the ventilation system of Abu-Tartur Mine(one of the largest underground mine in Egypt)has been studied.展开更多
BACKGROUND Multiple gastrointestinal stromal tumors(MGISTs)are a very rare type of gastrointestinal stromal tumor(GIST)and are usually observed in syndrome.AIM The paper aimed to describe the clinical and oncological ...BACKGROUND Multiple gastrointestinal stromal tumors(MGISTs)are a very rare type of gastrointestinal stromal tumor(GIST)and are usually observed in syndrome.AIM The paper aimed to describe the clinical and oncological features of MGISTs and to offer evidence for the diagnosis and treatment.METHODS Data of consecutive patients with MGISTs who were diagnosed at Peking University People’s Hospital(PKUPH)from 2008 to 2019 were retrospectively evaluated.Further,a literature search was conducted by retrieving data from PubMed,EMBASE,and the Cochrane library databases from inception up to November 30,2019.RESULTS In all,12 patients were diagnosed with MGISTs at PKUPH,and 43 published records were ultimately included following the literature review.Combined analysis of the whole individual patient data showed that female(59.30%),young(14.45%),and syndromic GIST(63.95%)patients comprised a large proportion of the total patient population.Tumors were mainly located in the small intestine(58.92%),and both CD117 and CD34 were generally positive.After a mean 78.32-mo follow-up,the estimated median overall survival duration(11.5 years)was similar to single GISTs,but recurrence-free survival was relatively poorer.CONCLUSION The clinical and oncological features are potentially different between MGISTs and single GIST.Further studies are needed to explore appropriate surgical approach and adjuvant therapy.展开更多
Experimental evidence is presented relevant to the angular dependences of multiple-bit upset （MBU） rates and patterns in static random access memories （SRAMs） under heavy ion irradiation. The single event upset （...Experimental evidence is presented relevant to the angular dependences of multiple-bit upset （MBU） rates and patterns in static random access memories （SRAMs） under heavy ion irradiation. The single event upset （SEU） cross sections under tilted ion strikes are overestimated by 23.9%-84.6%, compared with under normally incident ion with the equivalent linear energy transfer （LET） value of 41 MeV/（mg/cm2）, which can be partially explained by the fact that the MBU rate for tilted ions of 30° is 8.5%-9.8% higher than for normally incident ions. While at a lower LET of - 9.5 MeV/（mg/cm2）, no clear discrepancy is observed. Moreover, since the ion trajectories at normal and tilted incidences are different, the predominant double-bit upset （DBU） patterns measured are different in both conditions. Those differences depend on the LET values of heavy ions and devices under test. Thus, effective LET method should be used carefully in ground-based testing of single event effects （SEE） sensitivity, especially in MBU-sensitive devices.展开更多
Zero-crossing of a derivative of Gaussian filter is a well-known edge location criterion. Examples are the Laplacian, the second derivative in the gradient direction (SDGD) and the sum of the Laplacian and SDGD (PLUS)...Zero-crossing of a derivative of Gaussian filter is a well-known edge location criterion. Examples are the Laplacian, the second derivative in the gradient direction (SDGD) and the sum of the Laplacian and SDGD (PLUS). Derivative operators can easily be implemented by convoluting the primitive image with a derivative of a Gaussian. Gaussian filter displaces the equipotential of half height inwards for convex edge and outwards for concave edges. A Difference-of-Gaussian (DoG) filter is similar to the Laplacian-of-Gaussian but with opposite sign and causes a convex edge shift inwards. This paper introduces the Multiple-of-Gaussian niters to reduce curvature-based location error. Using a linear combination of N Gaussians(N】2) with proper weights, the edge shifts can be reduced to 1/(2N-3) of the ones produced by a similar Laplacian-of-Gaussian filter.展开更多
This paper applies the multiple ellipsoid model to the 16Ne （20Ne, 28Ne, 34Ne）-Na2 collision systems, and calcu- lates integral cross sections for rotational excitation at the incident energy of 190 meV. It can be s...This paper applies the multiple ellipsoid model to the 16Ne （20Ne, 28Ne, 34Ne）-Na2 collision systems, and calcu- lates integral cross sections for rotational excitation at the incident energy of 190 meV. It can be seen that the accuracy of the integral cross sections can be improved by increasing the number of equipotential ellipsoid surfaces. Moreover, by analysing the differences of these integral cross sections, it obtains the change rules of the integral cross sections with the increase of rotational angular quantum number j＇, and with the change of the mass of isotope substitution neon atom. Finally, the contribution of different regions of the potential to inelastic cross sections for 20Ne-Na2 collision system is investigated at relative incident energy of 190 meV.展开更多
The hydrodynamics of a single jet and four tandem jets in a cross flow are simulated by using the Computational Fluid Dynamics （CFD） software Fluent. The realizable model is used to close the Reynolds-Averaged equat...The hydrodynamics of a single jet and four tandem jets in a cross flow are simulated by using the Computational Fluid Dynamics （CFD） software Fluent. The realizable model is used to close the Reynolds-Averaged equations. The flow characteristics of the jets, including the jet trajectory, the velocity field and the turbulent kinetic energy are obtained with various jet-to-cross flow velocity ratios in the range of 2.38-17.88. It is shown that a single jet penetrates slightly deeper than the first jet in a jet group at the same , although the difference decreases with the decrease of . It is also found that the way in which the velo-city decays along the centerline of the jet is similar for both a single jet and the first jet in a group, and the speed of the decay increases with the decrease of . The downstream jets in a group are found to behave differently due to the sheltering effect of the first jet in the group. Compared with the first jet, the downstream jets penetrate deeper into the cross flow, and the velocity decays more slowly. The circulation zone between the two upstream jets in the front is stronger than those formed between the downstream jets. The Turbulent Kinetic Energy （TKE） sees a distinct double-peak across the cross-sections close to each nozzle, with low values in the jet core and high values in the shear layers. The double-peak gradually vanishes, as the shear layers of the jet merge further away from the nozzle, where the TKE assumes peaks at the jet centerline.展开更多
文摘Pseudomonas aeruginosa(P:aeruginosa)is a major opportunistic pathogen in hospital-acquired infections.Thus,carly diagnosis is the best strategy for fighting against these infections.In this report,we incorporated multiple crOsS displacement amplification(MCDA)combined with the malachite green(MG)for rapid,sensitive,specific and visual detection of P.aeruginosa by targeting the oprl gene.The MCDA-MG assay was conducted at 67°C for only 40 min during the amplification stage,and then products were directly detected by using colorimetric indicators(MG),eliminating the use of an electrophoresis instrument or amplicon analysis equipment.The entire process,including specimen processing(35 min),amplification(40 min)and detection(5 min),can be finished within 80 min.All 30 non-P.aeruginosa strains tested negative,indicating the high specificity of the MCDA primers.The analytical sensitivity of the MCDA-MG assay was 100 fg of genomic templates per reaction in pure culture,which was in complete accordance with MCDA by gel clectrophoresis and real-time turbidity.The assay was also successfully applied to detecting P.aeruginosa in stool samples.Therefore,the rapidity,simplicity,and nearly equipment free platform of the MCDA-MG technique make it possible for clinical diagnosis,and more.
文摘In large mines,single fan is usually not enough to ventilate all the working areas.Single mine-fan approach cannot be directly applied to multiple-fan networks because the present of multiple pressures and air quantities associated with each fan in the network.Accordingly,each fan in a multiple-fan system has its own mine characteristic curve,or a subsystem curve.Under some consideration,the conventional concept of a mine characteristic curve of a single-fan system can be directly extended to that of a particular fan within a multiple-fan system.In this paper the mutual effect of the fans on each other and their effect on the stability of the ventilation network were investigated by Hardy Cross algorithm combined with a switching-parameters technique.To show the validity and reliability of this algorithm,the stability of the ventilation system of Abu-Tartur Mine(one of the largest underground mine in Egypt)has been studied.
文摘BACKGROUND Multiple gastrointestinal stromal tumors(MGISTs)are a very rare type of gastrointestinal stromal tumor(GIST)and are usually observed in syndrome.AIM The paper aimed to describe the clinical and oncological features of MGISTs and to offer evidence for the diagnosis and treatment.METHODS Data of consecutive patients with MGISTs who were diagnosed at Peking University People’s Hospital(PKUPH)from 2008 to 2019 were retrospectively evaluated.Further,a literature search was conducted by retrieving data from PubMed,EMBASE,and the Cochrane library databases from inception up to November 30,2019.RESULTS In all,12 patients were diagnosed with MGISTs at PKUPH,and 43 published records were ultimately included following the literature review.Combined analysis of the whole individual patient data showed that female(59.30%),young(14.45%),and syndromic GIST(63.95%)patients comprised a large proportion of the total patient population.Tumors were mainly located in the small intestine(58.92%),and both CD117 and CD34 were generally positive.After a mean 78.32-mo follow-up,the estimated median overall survival duration(11.5 years)was similar to single GISTs,but recurrence-free survival was relatively poorer.CONCLUSION The clinical and oncological features are potentially different between MGISTs and single GIST.Further studies are needed to explore appropriate surgical approach and adjuvant therapy.
基金supported by the National Natural Science Foundation of China(Grant Nos.11179003,10975164,10805062,and 11005134)
文摘Experimental evidence is presented relevant to the angular dependences of multiple-bit upset （MBU） rates and patterns in static random access memories （SRAMs） under heavy ion irradiation. The single event upset （SEU） cross sections under tilted ion strikes are overestimated by 23.9%-84.6%, compared with under normally incident ion with the equivalent linear energy transfer （LET） value of 41 MeV/（mg/cm2）, which can be partially explained by the fact that the MBU rate for tilted ions of 30° is 8.5%-9.8% higher than for normally incident ions. While at a lower LET of - 9.5 MeV/（mg/cm2）, no clear discrepancy is observed. Moreover, since the ion trajectories at normal and tilted incidences are different, the predominant double-bit upset （DBU） patterns measured are different in both conditions. Those differences depend on the LET values of heavy ions and devices under test. Thus, effective LET method should be used carefully in ground-based testing of single event effects （SEE） sensitivity, especially in MBU-sensitive devices.
文摘Zero-crossing of a derivative of Gaussian filter is a well-known edge location criterion. Examples are the Laplacian, the second derivative in the gradient direction (SDGD) and the sum of the Laplacian and SDGD (PLUS). Derivative operators can easily be implemented by convoluting the primitive image with a derivative of a Gaussian. Gaussian filter displaces the equipotential of half height inwards for convex edge and outwards for concave edges. A Difference-of-Gaussian (DoG) filter is similar to the Laplacian-of-Gaussian but with opposite sign and causes a convex edge shift inwards. This paper introduces the Multiple-of-Gaussian niters to reduce curvature-based location error. Using a linear combination of N Gaussians(N】2) with proper weights, the edge shifts can be reduced to 1/(2N-3) of the ones produced by a similar Laplacian-of-Gaussian filter.
基金Project supported by the National Natural Science Foundation of China （Grant Nos. 10974139 and 10964002）, the Doctoral Program Foundation of Institution of Higher Education of China （Grant No. 20050610010）, the Science Technology Foundation of Guizhou Province of China （Grant No. [2009]2066） and Project of Aiding Elites＇ Research Condition of Guizhou Province （Grant No. TZJF-2008-42）.
文摘This paper applies the multiple ellipsoid model to the 16Ne （20Ne, 28Ne, 34Ne）-Na2 collision systems, and calcu- lates integral cross sections for rotational excitation at the incident energy of 190 meV. It can be seen that the accuracy of the integral cross sections can be improved by increasing the number of equipotential ellipsoid surfaces. Moreover, by analysing the differences of these integral cross sections, it obtains the change rules of the integral cross sections with the increase of rotational angular quantum number j＇, and with the change of the mass of isotope substitution neon atom. Finally, the contribution of different regions of the potential to inelastic cross sections for 20Ne-Na2 collision system is investigated at relative incident energy of 190 meV.
基金supported by the National Natural Science Foundation of China (Grant Nos. 50879020, 51179055 and 51125034)the Special Fund of State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering (Grant No. 2010585512)the Fundamental Research Funds for the Central Universities (Grant No. 2009B07614)
文摘The hydrodynamics of a single jet and four tandem jets in a cross flow are simulated by using the Computational Fluid Dynamics （CFD） software Fluent. The realizable model is used to close the Reynolds-Averaged equations. The flow characteristics of the jets, including the jet trajectory, the velocity field and the turbulent kinetic energy are obtained with various jet-to-cross flow velocity ratios in the range of 2.38-17.88. It is shown that a single jet penetrates slightly deeper than the first jet in a jet group at the same , although the difference decreases with the decrease of . It is also found that the way in which the velo-city decays along the centerline of the jet is similar for both a single jet and the first jet in a group, and the speed of the decay increases with the decrease of . The downstream jets in a group are found to behave differently due to the sheltering effect of the first jet in the group. Compared with the first jet, the downstream jets penetrate deeper into the cross flow, and the velocity decays more slowly. The circulation zone between the two upstream jets in the front is stronger than those formed between the downstream jets. The Turbulent Kinetic Energy （TKE） sees a distinct double-peak across the cross-sections close to each nozzle, with low values in the jet core and high values in the shear layers. The double-peak gradually vanishes, as the shear layers of the jet merge further away from the nozzle, where the TKE assumes peaks at the jet centerline.
