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Three-point bending behavior of aluminum foam sandwich with steel panel 被引量:7
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作者 祖国胤 卢日环 +4 位作者 李小兵 仲照阳 马幸江 韩明博 姚广春 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2013年第9期2491-2495,共5页
Static three-point bending tests of aluminum foam sandwiches with glued steel panel were performed. The deformation and failure of sandwich structure with different thicknesses of panel and foam core were investigated... Static three-point bending tests of aluminum foam sandwiches with glued steel panel were performed. The deformation and failure of sandwich structure with different thicknesses of panel and foam core were investigated. The results indicate that the maximum bending load increases with the thickness of both steel panel and foam core. The failure of sandwich can be ascribed to the crush and shear damage of foam core and the delamination of glued interface at a large bending load, The crack on the foam wall developed in the melting foam procedure is the major factor for the failure of foam core. The sandwich structure with thick foam core and thin steel panel has the optimal specific bending strength. The maximum bending load of that with 8 mm panel and 50 mm foam core is 66.06 kN. 展开更多
关键词 aluminum foam sandwich three-point bending failure mode panel thickness
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Three-point bending performance of a new aluminum foam composite structure 被引量:6
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作者 王宁珍 陈祥 +3 位作者 李奡 李言祥 张华伟 刘源 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2016年第2期359-368,共10页
A new composite structure based on aluminum foam sandwich and fiber metal laminate was proposed. A layer of glass fiber was provided at the interface between the metal panel and the aluminum foam core in this composit... A new composite structure based on aluminum foam sandwich and fiber metal laminate was proposed. A layer of glass fiber was provided at the interface between the metal panel and the aluminum foam core in this composite structure, using adhesive technology to bond the materials together by organic glue in the sequence of metal panel, glass fiber, aluminum foam core, glass fiber and metal panel. The experimental results show that the new composite structure has an improved comprehensive performance compared with the traditional aluminum foam sandwiches. The optimized parameters for the fabrication of the new aluminum foam composite structure with best bending strength were obtained. The epoxy resin and low porosity aluminum foams are preferred, the thickness of aluminum sheets should be at least 1.5 mm, and the type of glass fiber has little effect on the bending strength. The main failure modes of the new composite structures with two types of glues were discussed. 展开更多
关键词 composite structure three-point bending strength aluminum foam sandwich glass fiber
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Three-point bending of honeycomb sandwich beams with facesheet perforations 被引量:3
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作者 Pengbo Su Bin Han +2 位作者 Zhongnan Zhao Qiancheng Zhang Tian Jian Lu 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2018年第4期667-675,共9页
A novel square honeycomb-cored sandwich beam with perforated bottom facesheet is investigated under threepoint bending,both analytically and numerically.Perforated square holes in the bottom facesheet are characterize... A novel square honeycomb-cored sandwich beam with perforated bottom facesheet is investigated under threepoint bending,both analytically and numerically.Perforated square holes in the bottom facesheet are characterized by the area ratio of the hole to intact facesheet(perforation ratio).While for large-scale engineering applications like the decks of cargo vehicles and transportation ships,the perforations are needed to facilitate the fabrication process(e.g.,laser welding)as well as service maintenance,it is demonstrated that these perforations,when properly designed,can also enhance the resistance of the sandwich to bending.For illustration,fair comparisons among competing sandwich designs having different perforation ratios but equal mass is achieved by systematically thickening the core webs.Further,the perforated sandwich beam is designed with a relatively thick facesheet to avoid local indention failure so that it mainly fails in two competing modes:(1)bending failure,i.e.,yielding of beam cross-section and buckling of top facesheet caused by bending moment;(2)shear failure,i.e.,yielding and buckling of core webs due to shear forcing.The sensitivity of the failure loads to the ratio of core height to beam span is also discussed for varying perforation ratios.As the perfo-ration ratio is increased,the load of shear failure increases due to thickening core webs,while that of bending failure decreases due to the weakening bottom facesheet.Design of a sandwich beam with optimal perforation ratio is realized when the two failure loads are equal,leading to significantly enhanced failure load(up to 60%increase)relative to that of a non-perforated sandwich beam with equal mass. 展开更多
关键词 Honeycomb sandwich Facesheet perforation three-point bending Analytical model
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A CATASTROPHE ANALYSIS ON THE STABILITY OF THE CRACK GROWTH IN THREE-POINT BENDING SPECIMENS 被引量:2
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作者 Wei Demin Fan Xuejun, Taiyuan Unversity of Technology, Taiyuan 030024 《Acta Mechanica Solida Sinica》 SCIE EI 1996年第2期179-183,共5页
This paper presents an attempt at the application of catastrophe theory to the stability analysis of J-controlled crack growth in three-point bending specimens. By introducing the solutions of J-integral in the comple... This paper presents an attempt at the application of catastrophe theory to the stability analysis of J-controlled crack growth in three-point bending specimens. By introducing the solutions of J-integral in the completely yielding state for the ideal plastic material, the critical condition of losing stability for the crack propagation in the specimen is obtained, based on the cusp catastrophe theory. The process of the crack growth from geometrical sense is described. 展开更多
关键词 crack growth STABILITY cusp catastrophe J-INTEGRAL three-point bending specimen
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基于IP联合蛋白质组学探讨ENG互作蛋白在bEnd.3细胞中的作用
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作者 陈清杰 李奥迪 +4 位作者 季慧敏 孙艺瑄 蒋越 叶子奇 潘荣 《湖北科技学院学报(医学版)》 2024年第6期461-466,F0002,共7页
目的本研究拟筛选并验证在脑微血管内皮细胞(bEnd.3)中Endoglin(ENG)的互作蛋白及其作用。方法采用蛋白质组学和生物信息学技术鉴定并富集ENG互作蛋白,对这些互作蛋白进行亚细胞定位、GO功能注释、IPR注释和蛋白互作网络等分析。将以上... 目的本研究拟筛选并验证在脑微血管内皮细胞(bEnd.3)中Endoglin(ENG)的互作蛋白及其作用。方法采用蛋白质组学和生物信息学技术鉴定并富集ENG互作蛋白,对这些互作蛋白进行亚细胞定位、GO功能注释、IPR注释和蛋白互作网络等分析。将以上分析筛选出的ENG互作蛋白,采用免疫共沉淀(IP)实验、细胞免疫荧光(IF)和蛋白免疫印迹(WB)技术进行验证和对比,探究ENG互作蛋白在棕榈酸(PA)、基因沉默和过表达ENG处理bEnd.3细胞中的表达变化及作用。结果通过蛋白质组学分析,bEnd.3细胞中ENG互作蛋白在亚细胞定位分析中细胞骨架相关成分呈现出较高的占比;通过GO注释和IPR注释进一步发现,是细胞骨架中的中间纤维和微丝蛋白呈现较高的富集状态。ENG与细胞骨架蛋白互作热力图结果显示,与对照组相比,PA处理的bEnd.3细胞中微丝蛋白Filamin B(Flnb)数值显著降低。IP和IF结果显示,bEnd.3细胞中ENG与Flnb存在蛋白互作和共定位情况。WB结果显示,bEnd.3细胞在PA处理条件下,ENG和Flnb蛋白表达均下降;在ENG沉默条件下,Flnb蛋白随ENG表达的减少而降低;在ENG过表达条件下,Flnb蛋白随ENG表达的增加而升高。结论ENG互作蛋白广泛参与到多种相关的生物途径以及信号通路之中,ENG在bEnd.3细胞中与Flnb蛋白存在相互作用且二者具有共定位现象,ENG还能够调控PA处理的bEnd.3细胞Flnb蛋白表达水平。 展开更多
关键词 ENG Flnb 互作蛋白 bend.3 棕榈酸
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Experimental and theoretical analyses of package-on-package structure under three-point bending loading
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作者 贾苏 王习术 任淮辉 《Chinese Physics B》 SCIE EI CAS CSCD 2012年第12期346-354,共9页
High density packaging is developing toward miniaturization and integration, which causes many difficulties in designing, manufacturing, and reliability testing. Package-on-Package (POP) is a promising three-dimensi... High density packaging is developing toward miniaturization and integration, which causes many difficulties in designing, manufacturing, and reliability testing. Package-on-Package (POP) is a promising three-dimensional high- density packaging method that integrates a chip scale package (CSP) in the top package and a fine-pitch ball grid array (FBGA) in the bottom package. In this paper, in-situ scanning electron microscopy (SEM) observation is carried out to detect the deformation and damage of the PoP structure under three-point bending loading. The results indicate that the cracks occur in the die of the top package, then cause the crack deflection and bridging in the die attaching layer. Furthermore, the mechanical principles are used to analyse the cracking process of the PoP structure based on the multi-layer laminating hypothesis and the theoretical analysis results are found to be in good agreement with the experimental results. 展开更多
关键词 high density package in-situ SEM observation three-point bending multi-layer lami- nating hypothesis
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Modelling and Simulation on the Effect of Hot Forming Damage on Three-Point Bending Performance of Beam Components
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作者 Weimin Zhuang Pengyue Wang +2 位作者 Yang Liu Dongxuan Xie Hongda Shi 《Journal of Beijing Institute of Technology》 EI CAS 2020年第3期399-409,共11页
The effects of forming damage are analyzed,which occur during hot stamping process,on the load-carrying capacity and failure mode of hot stamped beams.A damage-coupled pre-forming constitutive model was proposed,in wh... The effects of forming damage are analyzed,which occur during hot stamping process,on the load-carrying capacity and failure mode of hot stamped beams.A damage-coupled pre-forming constitutive model was proposed,in which the damage during hot stamping process was introduced into the service response.The constitutive model was applied into the three-point bending simulation of a hot stamped beam,and then the influences of forming damage on the load-carrying capacity and cracks propagation were investigated.The results show that the forming damage reduces the maximum load capacity of the hot stamped beam by 7.5%.It also causes the crack to occur earlier and promotes crack to propagate along the radial direction of the punch. 展开更多
关键词 constitutive modelling hot-stamped beam forming damage three-point bending crack propagation
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Experimental,Numerical,and Analytical Studies on the Bending of Mechanically Lined Pipe 被引量:1
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作者 WEI Wen-bin YUAN Lin +1 位作者 ZHOU Jia-sheng LIU Zheng 《China Ocean Engineering》 SCIE EI CSCD 2024年第2期221-232,共12页
Mechanically lined pipe(MLP)is often used for offshore oil and gas transport because of its low cost and corrosion resistance.During installation and operation,the pipe may undergo severe bending deformation,which cau... Mechanically lined pipe(MLP)is often used for offshore oil and gas transport because of its low cost and corrosion resistance.During installation and operation,the pipe may undergo severe bending deformation,which causes the liner to separate from the outer pipe and buckles,affecting the stability of the whole line.In this paper,the buckling response of MLP subjected to bending is investigated to clarify its bending characteristics by employing both experiments,numerical simulation,as theoretical methods.Two types of MLPs were manufactured with GB 45 carbon steel(SLP)and Al 6061(ALP)used as the outer pipe material,respectively.The hydraulic expansion and bending experiments of small-scale MLPs are conducted.In addition to the ovalized shape of the cross-section for the SLP specimens,the copper liner was found to wrinkle on the compressive side.In contrast,the liner of ALP remains intact without developing any wrinkling and collapse mode.In addition,a dedicated numerical framework and theoretical models were also established.It was found both the manufacturing and bending responses of the MLP can be well reproduced,and the predicted maximum moment and critical curvatures are in good agreement with the experimental results. 展开更多
关键词 lined pipe bendING nonlinear ring theory BUCKLING PLASTICITY
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Effect of characteristics and distribution of Mg_(17)Al_(12)precipitates on tensile and bending properties of high-Al-containing Mg alloys 被引量:1
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作者 Sumi Jo Gyo Myeong Lee +2 位作者 Jong Un Lee Young Min Kim Sung Hyuk Park 《Journal of Magnesium and Alloys》 SCIE EI CAS CSCD 2024年第2期779-793,共15页
This study investigates the effect of characteristics and distribution of Mg_(17)Al_(12)precipitates on the uniaxial tensile and three-point bending properties of extruded Mg alloys containing high Al contents.The ext... This study investigates the effect of characteristics and distribution of Mg_(17)Al_(12)precipitates on the uniaxial tensile and three-point bending properties of extruded Mg alloys containing high Al contents.The extruded Mg–9Al–1Zn–0.3Mn(AZ91)alloy contains lamellar-structured Mg_(17)Al_(12)discontinuous precipitates along the grain boundaries,which are formed via static precipitation during natural air cooling.The extruded Mg–11Al–1Zn–0.3Mn(AZ111)alloy contains spherical Mg_(17)Al_(12)precipitates at the grain boundaries and inside the grains,which are formed via dynamic precipitation during extrusion.Due to inhomogeneous distribution of precipitates,the AZ111 alloy consists of two different precipitate regions:precipitate-rich region with numerous precipitates and finer grains and precipitate-scarce region with a few precipitates and coarser grains.The AZ111 alloy exhibits a higher tensile strength than the AZ91 alloy because its smaller grain size and more abundant precipitates result in stronger grain-boundary hardening and precipitation hardening effects,respectively.However,the tensile elongation of the AZ111 alloy is lower than that of the AZ91 alloy because the weak cohesion between the dynamic precipitates and the matrix facilitates the crack initiation and propagation.During bending,a macrocrack initiates on the outer surface of bending specimen in both alloys.The AZ111 alloy exhibits higher bending yield strength and lower failure bending strain than the AZ91 alloy.The bending specimens of the AZ91 alloy have similar bending formability,whereas those of the AZ111 alloy exhibit considerable differences in bending formability and crack propagation behavior,depending on the distribution and number density of precipitates in the specimen.In bending specimens of the AZ111 alloy,it is found that the failure bending strain(ε_(f,bending))is inversely proportional to the area fraction of precipitates in the outer zone of bending specimen(A_(ppt)),with a relationship ofε_(f,bending)=–0.1A_(ppt)+5.86. 展开更多
关键词 Mg–Al alloy EXTRUSION bendING Precipitation Microstructure
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The Applied Research of Fracture in Three-point Bending to Blanking of Bearing Steel
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作者 Youtang Li Ping Ma Changfeng Yan 《上海交通大学学报》 EI CAS CSCD 北大核心 2004年第z2期199-205,共7页
The application of fracture in three-point bending to blanking of bearing steel is studied in this paper. Several mechanical models of stress blanking are discussed at first. And then the experiments have been made in... The application of fracture in three-point bending to blanking of bearing steel is studied in this paper. Several mechanical models of stress blanking are discussed at first. And then the experiments have been made in special equipment of three-point bending designed by us. Several problems, such as the suitable span, the relations between bending and tension bending complex, the ratio of blanking length to the diameter, the relations of blank length to force, the feature of fracture, are studied through the experiments. The suitable parameters of three-point bending to blanking, depth and tip radius of notch and the ratio between blanking length and stick diameter have been proposed. 展开更多
关键词 BEARING steel three POINTS bendING BLANKING CRACK technique FRACTURE design
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Dynamic Fracture Behaviors of Selected Aluminum Alloys Under Three-point Bending 被引量:2
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作者 Ming-zhi XING Yong-gang WANG Zhao-xiu JIANG 《Defence Technology(防务技术)》 SCIE EI CAS 2013年第4期193-200,共8页
The dynamic fracture behaviors of the extruded 2024-T4 and 7075-T6 aluminum alloys are investigated by using an instrumented drop tower machine.The specimens are made from a 25 mm diameter extruded circular rod.The dy... The dynamic fracture behaviors of the extruded 2024-T4 and 7075-T6 aluminum alloys are investigated by using an instrumented drop tower machine.The specimens are made from a 25 mm diameter extruded circular rod.The dynamic three-point bending tests of each alloy are carried out at different impact velocities.The initiation fracture toughness and average propagation fracture toughness of 2024-T4 and 7075-T6 are determined at different loading rates.The results show that both the initiation toughness and the propagation toughness increase with the loading rate.Further,the difference between the fracture toughness behaviors of 2024-T4 and 7075-T6 is found to be dependent on the variation of fracture mechanism.The comprehensive fractographic investigations of the fracture surfaces clearly demonstrate that the fracture mode of 2024-T4 is predominantly transgranular fracture with high density small-sized dimples,and the fracture mode of 7075-T6 is mainly intergranular fracture with many intermetallic particles in the bottom of voids located in the fracture surface. 展开更多
关键词 动态断裂行为 三点弯曲试验 铝合金 行为研究 断裂韧性 加载速率 断裂模式 IRMA
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Discharge evolution law of debris flow based on a sharp bend physical modeling test
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作者 LU Ming SUN Hao +3 位作者 LIU Jinfeng Abrar HUSSAIN SHANG Yuqi FU Hang 《Journal of Mountain Science》 SCIE CSCD 2024年第6期1904-1915,共12页
For the basins with debris flow development,its channel terrain exhibits a tortuous shape,which significantly restricts the movement of debris flows and leads to severe erosion effects on the concave bank.Therefore,th... For the basins with debris flow development,its channel terrain exhibits a tortuous shape,which significantly restricts the movement of debris flows and leads to severe erosion effects on the concave bank.Therefore,this study aims to analyze the shear force of debris flows within the bend channel.We established the relationship between the shear force and bend curvature through laboratory experiments.Under the long-term erosion by debris flows,the curvature radius of bends gradually increases,however,when this increasing trend reaches an equilibrium state with the intensity of debris flow discharge,there will be no significant change in curvature radius.In general,the activity pattern and discharges of debris flows would remain relatively stable.Hence,we can infer the magnitude of debris flow discharges from the terrain parameters of the bend channel. 展开更多
关键词 Debris flow discharge Erosion effect bend channel Curvature radius
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Bending Failure Mode and Prediction Method of the Compressive Strain Capacity of A Submarine Pipeline with Dent Defects
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作者 HOU Fu-heng JIA Lu-sheng +3 位作者 CHEN Yan-fei ZHANG Qi ZHONG Rong-feng WANG Chun-sha 《China Ocean Engineering》 SCIE EI CSCD 2024年第4期636-647,共12页
A dent is a common type of defects for submarine pipeline.For submarine pipelines,high hydrostatic pressure and internal pressure are the main loads.Once pipelines bend due to complex subsea conditions,the compression... A dent is a common type of defects for submarine pipeline.For submarine pipelines,high hydrostatic pressure and internal pressure are the main loads.Once pipelines bend due to complex subsea conditions,the compression strain capacity may be exceeded.Research into the local buckling failure and accurate prediction of the compressive strain capacity are important.A finite element model of a pipeline with a dent is established.Local buckling failure under a bending moment is investigated,and the compressive strain capacity is calculated.The effects of different parameters on pipeline local buckling are analyzed.The results show that the dent depth,external pressure and internal pressure lead to different local buckling failure modes of the pipeline.A higher internal pressure indicates a larger compressive strain capacity,and the opposite is true for external pressure.When the ratio of external pressure to collapse pressure of intact pipeline is greater than 0.1,the deeper the dent,the greater the compressive strain capacity of the pipeline.And as the ratio is less than 0.1,the opposite is true.On the basis of these results,a regression equation for predicting the compressive strain capacity of a dented submarine pipeline is proposed,which can be referred to during the integrity assessment of a submarine pipeline. 展开更多
关键词 submarine pipeline dent defect bending load local buckling compressive strain capacity
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Analysis of deformation mechanisms in magnesium single crystals using a dedicated four-point bending tester
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作者 Yutaka Yoshida Rikuto Izawa Kenji Ohkubo 《Journal of Magnesium and Alloys》 SCIE EI CAS CSCD 2024年第5期1911-1917,共7页
In this study,we explored the deformation mechanisms of Mg single crystals using a combination of scanning electron microscopy and electron backscattered diffraction in conjunction with a dedicated four-point bending ... In this study,we explored the deformation mechanisms of Mg single crystals using a combination of scanning electron microscopy and electron backscattered diffraction in conjunction with a dedicated four-point bending tester.We prepared two single-crystal samples,oriented along the<1120>and<1010>directions,to assess the mechanisms of deformation when the initial basal slip was suppressed.In the<1120>sample,the primary{1012}twin(T1)was confirmed along the<1120>direction of the sample on the compression side with an increase in bending stress.In the<1010>sample,T1 and the secondary twin(T2)were confirmed to be along the<1120>direction,with an orientation of±60°with respect to the bending stress direction,and their direction matched with(0001)in T1 and T2.This result implies that crystallographically,the basal slip occurs readily.In addition,the<1010>sample showed the double twin in T1 on the compression side and the tertiary twin along the<1010>direction on the tension side.These results demonstrated that the maximum bending stress and displacement changed significantly under the bend loading because the deformation mechanisms were different for these single crystals.Therefore,the correlation between bending behavior and twin orientation was determined,which would be helpful for optimizing the bending properties of Mg-based materials. 展开更多
关键词 Four-point bending Magnesium single crystal TWINNING Basal slip Scanning electron microscopy Electron backscatter diffraction
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Bending strength degradation of a cantilever plate with surface energy due to partial debonding at the clamped boundary
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作者 Zhenliang HU Xueyang ZHANG Xianfang LI 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI CSCD 2024年第9期1573-1594,共22页
This paper investigates the bending fracture problem of a micro/nanoscale cantilever thin plate with surface energy,where the clamped boundary is partially debonded along the thickness direction.Some fundamental mecha... This paper investigates the bending fracture problem of a micro/nanoscale cantilever thin plate with surface energy,where the clamped boundary is partially debonded along the thickness direction.Some fundamental mechanical equations for the bending problem of micro/nanoscale plates are given by the Kirchhoff theory of thin plates,incorporating the Gurtin-Murdoch surface elasticity theory.For two typical cases of constant bending moment and uniform shear force in the debonded segment,the associated problems are reduced to two mixed boundary value problems.By solving the resulting mixed boundary value problems using the Fourier integral transform,a new type of singular integral equation with two Cauchy kernels is obtained for each case,and the exact solutions in terms of the fundamental functions are determined using the PoincareBertrand formula.Asymptotic elastic fields near the debonded tips including the bending moment,effective shear force,and bulk stress components exhibit the oscillatory singularity.The dependence relations among the singular fields,the material constants,and the plate's thickness are analyzed for partially debonded cantilever micro-plates.If surface energy is neglected,these results reduce the bending fracture of a macroscale partially debonded cantilever plate,which has not been previously reported. 展开更多
关键词 micro/nanoscale cantilever plate partially debonded bending fracture singular integral equation oscillatory singularity
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A Light and Simplified Branch Bending Method for Young Pear Trees
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作者 Jintao XU Longfei LI +3 位作者 Minghui JI Huan LIU Lijuan GAO Baofeng HAO 《Plant Diseases and Pests》 2024年第2期19-21,共3页
Aiming at high cost and low efficiency of conventional branch bending method in the modern intensive planting and labor-saving cultivation mode of young pear trees,this paper provides a new branch bending method with ... Aiming at high cost and low efficiency of conventional branch bending method in the modern intensive planting and labor-saving cultivation mode of young pear trees,this paper provides a new branch bending method with wide source of raw materials,cheap price and simple operation,which is also suitable for the management of low-age branches in the process of high grafting and upgrading of traditional big trees. 展开更多
关键词 Pear tree Light and simplified Branch bending METHOD
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The Effects of the Longitudinal Axis of Loading upon Bending, Shear and Torsion of a Thin-Walled Cantilever Channel Beam
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作者 David W. A. Rees Abdelraouf M. Sami Alsheikh 《World Journal of Mechanics》 2024年第5期73-96,共24页
Three aluminium channel sections of US standard extruded dimension are mounted as cantilevers with x-axis symmetry. The flexural bending and shear that arise with applied axial torsion are each considered theoreticall... Three aluminium channel sections of US standard extruded dimension are mounted as cantilevers with x-axis symmetry. The flexural bending and shear that arise with applied axial torsion are each considered theoretically and numerically in terms of two longitudinal axes of loading not coincident with the shear centre. In particular, the warping displacements, stiffness and stress distributions are calculated for torsion applied to longitudinal axes passing through the section’s centroid and its web centre. The stress conversions derived from each action are superimposed to reveal a net sectional stress distribution. Therein, the influence of the axis position upon the net axial and shear stress distributions is established compared to previous results for each beam when loading is referred to a flexural axis through the shear centre. Within the net stress analysis is, it is shown how the constraint to free warping presented by the end fixing modifies the axial stress. The latter can be identified with the action of a ‘bimoment’ upon each thin-walled section. 展开更多
关键词 Thin-Aluminium Channels Cantilever Beam bending Shear Torsion WARPING BIMOMENT Flexural Axis Centre of Twist CENTROID Shear Centre Torsional Stiffness Constrained Stress
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基于p38MAPK-PPARγ/NF-κB信号通路探讨消肿止痛合剂对血管内皮细胞bEND-3凋亡的影响
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作者 朱克玉 宋渊 +4 位作者 梁旭东 沈稼轩 张团庄 乔靖 刘涛 《辽宁中医杂志》 CAS 北大核心 2024年第4期149-152,I0002,共5页
目的从细胞及信号通路层面探讨消肿止痛合剂对皮瓣缺血再灌注损伤中血管内皮细胞凋亡的影响。方法血管内皮细胞bEND-3培养。TNF-α体外细胞凋亡诱导模型建立并将细胞分为:p38MAPK抑制剂组、PPARγ抑制剂组、NF-κB抑制剂组、模型对照组... 目的从细胞及信号通路层面探讨消肿止痛合剂对皮瓣缺血再灌注损伤中血管内皮细胞凋亡的影响。方法血管内皮细胞bEND-3培养。TNF-α体外细胞凋亡诱导模型建立并将细胞分为:p38MAPK抑制剂组、PPARγ抑制剂组、NF-κB抑制剂组、模型对照组、空白组。微丝绿色荧光探针观察细胞骨架微丝。RT-PCR法检测血管内皮细胞bEND-3细胞中p38MAPK、PPARγ、IκBα、NF-κBmRNA的表达。结果空白组中p38MAPK、PPARγ、IκBα、NF-κB的表达量无明显差异,模型对照组中p38MAPK与NF-κB的表达量最高,与模型对照组相比,p38MAPK抑制剂组bEnd.3细胞中p38MAPK与NF-κB的表达被抑制,PPARγ、IκBα表达量升高(P<0.05);PPARγ抑制剂组中PPARγ、IκBα、NF-κB的表达被抑制,p38MAPK表达量升高(P<0.05),NF-κB抑制剂组中p38MAPK与NF-κB的表达被抑制,IκBα与PPARγ的表达量升高(P<0.05);与空白组相比,p38MAPK抑制剂组与NF-κB抑制剂组中p38MAPK、PPARγ、IκBα、NF-κB的表达量升高(P<0.05),PPARγ抑制剂组中p38MAPK与NF-κB表达量明显升高,PPARγ、IκBα表达被抑制(P<0.05)。结论消肿止痛合剂可抑制bEND-3细胞凋亡,其机制与影响可能与p38MAPK-PPARγ/NF-κB信号通路有关。 展开更多
关键词 消肿止痛合剂 bend-3细胞 信号通路 皮瓣
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Existence of Monotone Positive Solution for a Fourth-Order Three-Point BVP with Sign-Changing Green’s Function
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作者 Junrui Yue Yun Zhang Qingyue Bai 《Open Journal of Applied Sciences》 2024年第1期63-69,共7页
This paper is concerned with the following fourth-order three-point boundary value problem , where , we discuss the existence of positive solutions to the above problem by applying to the fixed point theory in cones a... This paper is concerned with the following fourth-order three-point boundary value problem , where , we discuss the existence of positive solutions to the above problem by applying to the fixed point theory in cones and iterative technique. 展开更多
关键词 Fourth-Order three-point Boundary Value Problem Sign-Changing Green’s Function Fixed Point Index Iterative Technique Monotone Positive Solution EXISTENCE
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小鼠脑微血管内皮细胞株bEnd.3的细胞特征及基因表达谱分析 被引量:13
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作者 乐飞 张国平 +2 位作者 金惠铭 史连国 殷莲华 《中国病理生理杂志》 CAS CSCD 北大核心 2004年第8期1340-1344,共5页
目的 :对一种小鼠脑组织来源的血管内皮细胞株bEnd .3的主要细胞特征进行研究并做血管内皮细胞生物学功能基因芯片分析。方法 :利用倒置显微镜、扫描和透射电子显微镜观察细胞形态特征 ;免疫细胞化学法显示血管内皮细胞特异性表面标志 ;... 目的 :对一种小鼠脑组织来源的血管内皮细胞株bEnd .3的主要细胞特征进行研究并做血管内皮细胞生物学功能基因芯片分析。方法 :利用倒置显微镜、扫描和透射电子显微镜观察细胞形态特征 ;免疫细胞化学法显示血管内皮细胞特异性表面标志 ;ELISA定量检测PGE2 ;流式细胞术、MTT法研究增殖和凋亡、血管内皮细胞基因芯片研究正常培养bEnd 3基因表达谱。结果 :bEnd 3具备多种典型的微血管内皮细胞 (MVEC)特征 :细胞呈多边形或梭型、呈铺路石样生长 ;存在管腔样结构 (TLS)和毛细血管样网络 ,细胞表面有丰富的微绒毛 ;vW因子、CD34阳性 ;CD31、CD36、CD10 5等也有不同程度的表达 ;细胞高水平分泌前列腺素E2 (PGE2 ) ;多种与正常血管功能密切相关的基因也有不同程度的表达。结论 :bEnd .3保留了MVEC的基本特性 ,可用于心脑血管疾病。 展开更多
关键词 bend.3细胞 内皮 von WILLEBRAND因子 抗原 CD34 寡核苷酸序列分析
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