A competitive indirect time-resolved fluoroimmunoassay(TRFIA) was developed for detection of zearalenone(ZEN) in cereals,in which ZEN conjugated to bovine serum albumin(BSA) is used as solid-phase antigen.A competitiv...A competitive indirect time-resolved fluoroimmunoassay(TRFIA) was developed for detection of zearalenone(ZEN) in cereals,in which ZEN conjugated to bovine serum albumin(BSA) is used as solid-phase antigen.A competitive indirect TRFIA was conducted by simultaneously incubating ZEN in standard or extracted samples with anti-ZEN monoclonal antibody over ZEN-BSA coated plates,and then determining the bound ZEN monoclonal antibody with goat anti-mouse europium conjugate.Samples were extracted with methanol/water...展开更多
Eu-chelate were used to construct a two-site sandwich-type assay for pepsinogen Ⅰ (PGI) with time-resolved fluoroimmunoassay (TRFIA) as a detection technique. On the noncompetitive assay, captured monoclonal anti...Eu-chelate were used to construct a two-site sandwich-type assay for pepsinogen Ⅰ (PGI) with time-resolved fluoroimmunoassay (TRFIA) as a detection technique. On the noncompetitive assay, captured monoclonal antibodies (McAbs) coated on wells were directed against a specific antigenic site on the PGI. Another McAbs, called as labeling McAbs, were prepared with the Eu-chelate of N-(p-isothiocyanatobenzyl)-diethylenetriamine-N, N, N, N-tetraacetic acid and directed against a different antigenic site on the PGI. The fluorescence counts of bound Eu^3+ -McAbs were measured with the auto DELFIA1235 system. The PGI in sera from healthy volunteers were determined by PGI-TRFIA. The within-run and between-run CVs of the PGI-TRFIA were 1.9% and 4.7%, respectively, and the recovery rate was 102.65%. The assay had a detection limit of 0.05 μg· L^-1. The PGI-TRFIA provided a linear response from 3.5 to 328 μg· L^-1. The cross-reacting rate with pepsinogen Ⅱ was negligible. The linear correlation of PGI-TRFIA and radioimmunassay measurements resulted in a correlation coefficient of 0.977. The means of healthy volunteers were 154 ±43 μg·L^-1 for serum PGI. The availability of a highly sensitive, reliable, and convenient method for quantifying PGI will allow investigations into the possible diagnostic value of this analyte in various clinical conditions, including gastric carcinoma, duodenal ulcer, gastritis and severe atrophic gastritis.展开更多
We measure the time-resolved terahertz spectroscopy of GeSn thin film and studied the ultrafast dynamics of its photo-generated carriers.The experimental results show that there are photo-generated carriers in GeSn un...We measure the time-resolved terahertz spectroscopy of GeSn thin film and studied the ultrafast dynamics of its photo-generated carriers.The experimental results show that there are photo-generated carriers in GeSn under femtosecond laser excitation at 2500 nm,and its pump-induced photoconductivity can be explained by the Drude–Smith model.The carrier recombination process is mainly dominated by defect-assisted Auger processes and defect capture.The firstand second-order recombination rates are obtained by the rate equation fitting,which are(2.6±1.1)×10^(-2)ps^(-1)and(6.6±1.8)×10^(-19)cm^(3)·ps^(-1),respectively.Meanwhile,we also obtain the diffusion length of photo-generated carriers in GeSn,which is about 0.4μm,and it changes with the pump delay time.These results are important for the GeSn-based infrared optoelectronic devices,and demonstrate that Ge Sn materials can be applied to high-speed optoelectronic detectors and other applications.展开更多
A novel dual-label time-resolved fluoroimmunoassay method was developed for the simultaneous determination of chloramphenicol (CAP) and ractopamine (RAC) residues in 18 swine tissue samples,using anti-CAP and anti-RAC...A novel dual-label time-resolved fluoroimmunoassay method was developed for the simultaneous determination of chloramphenicol (CAP) and ractopamine (RAC) residues in 18 swine tissue samples,using anti-CAP and anti-RAC monoclonal antibodies labeled with europium (Eu 3+) and samarium (Sm 3+),respectively.The detection limits for CAP and RAC were 0.06 and 0.25ng/g.The recovery from swine muscle samples was 102%-121% for CAP at spiking levels of 0.1-5ng/g,and 69.8%-85.8% for RAC at spiking levels of 1-10ng/g.The results obtained from the swine tissue samples using this method showed good agreement with those obtained using ELISA and GC-MS,with correlation coefficients (R) between 0.92-0.98.展开更多
A study of a nanosecond laser irradiation on the titanium-layer-buried gold planar target is presented. The timeresolved x-ray emission spectra of titanium tracer are measured by a streaked crystal spectrometer. By co...A study of a nanosecond laser irradiation on the titanium-layer-buried gold planar target is presented. The timeresolved x-ray emission spectra of titanium tracer are measured by a streaked crystal spectrometer. By comparing the simulated spectra obtained by using the FLYCHK code with the measured titanium spectra, the temporal plasma states, i.e.,the electron temperatures and densities, are deduced. To evaluate the feasibility of using the method for the characterization of Au plasma states, the deduced plasma states from the measured titanium spectra are compared with the Multi-1D hydrodynamic simulations of laser-produced Au plasmas. By comparing the measured and simulated results, an overall agreement for the electron temperatures is found, whereas there are deviations in the electron densities. The experiment–theory discrepancy may suggest that the plasma state could not be well reproduced by the Multi-1D hydrodynamic simulation, in which the radial gradient is not taken into account. Further investigations on the spectral characterization and hydrodynamic simulations of the plasma states are needed. All the measured and FLYCHK simulated spectra are given in this paper as datasets. The datasets are openly available at http://www.doi.org/10.57760/sciencedb.j00113.00032.展开更多
To quantitatively determine tylosin and tilmicosin in edible animal tissues,a time-resolved fluoroimmunoassay(TRFIA) has been developed and validated.For this purpose,desmycosin-O-carboxymethoxylamine-BSA was fixed on...To quantitatively determine tylosin and tilmicosin in edible animal tissues,a time-resolved fluoroimmunoassay(TRFIA) has been developed and validated.For this purpose,desmycosin-O-carboxymethoxylamine-BSA was fixed onto microtiter plates,standards and samples were loaded and,finally,diluted europium-labeled anti-tylosin antibodies were added.Results show that the limit of detection for tylosin was 0.03 ng mL-1 and that for tilmicosin was 0.05 ng mL-1.The recoveries were 73.6% to 120.5%,with coefficients of variation below 15.6% in various biological matrices spiked with tylosin and tilmicosin at concentrations of 50-200 ngg-1.There was good correlation(R2>0.99) between the TRFIA,an enzyme-linked immunosorbent assay and high performance liquid chromatography data.In conclusion,the new TRFIA is applicable to the detection of tylosin and tilmicosin and is an effective and economical method that will enable high-throughput sample screening.The method is expected to be widely applicable.展开更多
The combined use of chemometrics and chemiluminescence(CL)measurements,with the aid of the stopped-flow mixing technique,developed a simple time-resolved CL method for the simultaneous determination of captopril(CP...The combined use of chemometrics and chemiluminescence(CL)measurements,with the aid of the stopped-flow mixing technique,developed a simple time-resolved CL method for the simultaneous determination of captopril(CPL)and hydrochlorothiazide(HCT).The stopped-flow technique in a continuous-flow system was employed in this work in order to emphasize the kinetic differences between the two analytes in cerium(IV)-rhodamine 6G CL system.After the flow was stopped,an initial rise of CL signal was observed for HCT standards,while a direct decay of CL signal was obtained for CPL standards.The mixed CL signal was monitored and recorded on the whole process of continuous-flow/stopped-flow,and the obtained data were processed by the chemometric approach of artificial neural network.The relative prediction error(RPE)of CPL and HCT was 5.9% and 8.7%,respectively.The recoveries of CPL and HCT in tablets were found to fall in the range between 95% and 106%.The proposed method was successfully applied to the simultaneous determination of CPL and HCT in a compound pharmaceutical formulation.展开更多
Aluminum ablation by multiple femtosecond laser pulses is investigated via time-resolved shadowgraphs and scanning electron microscope (SEM) images of the ablation spot. The spatial distribution of the ejected mater...Aluminum ablation by multiple femtosecond laser pulses is investigated via time-resolved shadowgraphs and scanning electron microscope (SEM) images of the ablation spot. The spatial distribution of the ejected material and the radius of the shock wave generated during the ablation are found to vary with the increase in the number of pulses. In the initial two pulses, nearly concentric and semicircular stripes within the shock wave front are observed, unlike in subsequent pulses. Ablation by multiple femtosecond pulses exhibits different characteristics compared with the case induced by single femtosecond pulse because of the changes to the aluminum target surface induced by the preceding pulses.展开更多
The relationship between the in the logarithmic law (log-law) region of bursting event and the low/high-speed streak a turbulent boundary layer is investigated. A tomographic time-resolved particle image velocimetry...The relationship between the in the logarithmic law (log-law) region of bursting event and the low/high-speed streak a turbulent boundary layer is investigated. A tomographic time-resolved particle image velocimetry (TRPIV) system is used to measure the instantaneous three-dimensional-three-component (3D-3C) velocity field. The momentum thickness based Reynolds number is about 2 460. The topological information in the log-law region is obtained experimentally. It is found that the existence of the quadrupole topological structure implies a three-pair hairpin-like vortex packet, which is in connection with the low/high-speed streak. An idealized 3D topological model is then proposed to characterize the observed hairpin vortex packet and low/high-speed streak.展开更多
In broadband coherent anti-Stokes Raman scattering (CARS) spectroscopy with supercontinuum (SC), the simultaneously detectable spectral coverage is limited by the spectral continuity and the simultaneity of variou...In broadband coherent anti-Stokes Raman scattering (CARS) spectroscopy with supercontinuum (SC), the simultaneously detectable spectral coverage is limited by the spectral continuity and the simultaneity of various spectral components of SC in an enough bandwidth. By numerical simulations, the optimal experimental conditions for improving the SC are obtained. The broadband time-resolved CARS spectrography based on the SC with required temporal and spectral distributions is realised. The global molecular vibrational spectrum with well suppressed nonresonant background noise can be obtained in a single measurement. At the same time, the measurements of dephasing times of various molecular vibrational modes can be conveniently achieved from intensities of a sequence of time-resolved CARS signals. It will be more helpful to provide a complete picture of molecular vibrations, and to exhibit a potential to understand not only both the solvent dynamics and the solute-solvent interactions, but also the mechanisms of chemical reactions in the fields of biology, chemistry and material science.展开更多
Time-resolved measurement of atomic emission enhancement is performed by using a 500-fs KrF laser pulse incident upon a high density supersonic O2 gas jet, synchronized with an orthogonal ns frequency-doubled Nd:YAG ...Time-resolved measurement of atomic emission enhancement is performed by using a 500-fs KrF laser pulse incident upon a high density supersonic O2 gas jet, synchronized with an orthogonal ns frequency-doubled Nd:YAG laser pulse. The ultra-short pulse serves as an igniter of the gas jet, and the subsequent ns-laser pulse significantly enhances the atomic emission. Analysis shows that the contributions to the enhancement effect are made mainly by the bremsstrahlung radiation and cascade ionization.展开更多
We performed femtosecond time-resolved coherent anti-Stokes Raman scattering (fs-CARS) measurements on liquid toluene and PVK film. For both samples, we selectively excited the CH stretching vibrational modes and ob...We performed femtosecond time-resolved coherent anti-Stokes Raman scattering (fs-CARS) measurements on liquid toluene and PVK film. For both samples, we selectively excited the CH stretching vibrational modes and observed the expected quantum beat signals. The frequency of the well-defined beats is in good agreement with the energy difference between the two simultaneously excited modes, which demonstrates that a coherent coupling between the vibrational modes of the C H chemical bonds exists at the different positions of the molecules. The dephasing times of the excited modes are obtained simultaneously.展开更多
Dermatophagoides farinae and D. pteronyssinus are the prevalent house dust mites (HDM) in tropical countries and are associated with allergic diseases. This investigation developed a time- resolved immunofluorometric ...Dermatophagoides farinae and D. pteronyssinus are the prevalent house dust mites (HDM) in tropical countries and are associated with allergic diseases. This investigation developed a time- resolved immunofluorometric assay (TR-IFMA) for the first time to detect specific IgE antibody in patients with skin prick test positive to HDM but no detectable IgE by other means. Levels of IgE to natural and recombinant HDM allergens were measured by TR-IFMA in 50 HDM-allergic patients and 19 healthy participants compared to sandwich enzyme-linked immunosorbent assay (ELISA). A recombinant allergen, rDerf2, showed a 14 kDa band corresponding to broad range proteins of natural HDM.TR-IFMA showed sensitivity lower than 0.35 kUA/l. TR-IFMA employing three HDM antigens showed good correlations with sandwich ELISA at R2 0.93-0.96. TR-IFMA detected HDM IgE in 62, 62, 25 percent of allergic patient serum sample compared to 28, 32, and 22 percent detected by ELISA result using three HDM allergen. TR-IFMA also detected 26.3, 31.6, and 5.3 percent positive samples from 19 healthy participants while ELISA showed 0, 5.3, and 0 percents IgE positive samples. The use of rDerf2 as an HDM allergen for the assay was verified with no statistically different from other HDM allergens. TR-IFMA showed lower detection limit than ELISA and yielded higher sensitivity for serum of people with allergic symptoms with no detectable HDM IgE. It is anticipated that TR-IFMA for HDM-specific IgE detection will play an important role in future diagnosis of HDM allergy in clinical laboratories and for different research purposes.展开更多
One of the technical bottlenecks of traditional laser-induced breakdown spectroscopy(LIBS) is the difficulty in quantitative detection caused by the matrix effect. To troubleshoot this problem,this paper investigated ...One of the technical bottlenecks of traditional laser-induced breakdown spectroscopy(LIBS) is the difficulty in quantitative detection caused by the matrix effect. To troubleshoot this problem,this paper investigated a combination of time-resolved LIBS and convolutional neural networks(CNNs) to improve K determination in soil. The time-resolved LIBS contained the information of both wavelength and time dimension. The spectra of wavelength dimension showed the characteristic emission lines of elements, and those of time dimension presented the plasma decay trend. The one-dimensional data of LIBS intensity from the emission line at 766.49 nm were extracted and correlated with the K concentration, showing a poor correlation of R_c^2?=?0.0967, which is caused by the matrix effect of heterogeneous soil. For the wavelength dimension, the two-dimensional data of traditional integrated LIBS were extracted and analyzed by an artificial neural network(ANN), showing R_v^2?=?0.6318 and the root mean square error of validation(RMSEV)?=?0.6234. For the time dimension, the two-dimensional data of time-decay LIBS were extracted and analyzed by ANN, showing R_v^2?=?0.7366 and RMSEV?=?0.7855.These higher determination coefficients reveal that both the non-K emission lines of wavelength dimension and the spectral decay of time dimension could assist in quantitative detection of K.However, due to limited calibration samples, the two-dimensional models presented over-fitting.The three-dimensional data of time-resolved LIBS were analyzed by CNNs, which extracted and integrated the information of both the wavelength and time dimension, showing the R_v^2?=?0.9968 and RMSEV?=?0.0785. CNN analysis of time-resolved LIBS is capable of improving the determination of K in soil.展开更多
Ultrafast dissociation dynamics of chloroiodomethane (CH2ICl) in the B band is studied by femtosecond time- resolved time-of-flight (TOF) mass spectrometry. Time-resolved TOF mass signal of parent ion (CH2ICl+)...Ultrafast dissociation dynamics of chloroiodomethane (CH2ICl) in the B band is studied by femtosecond time- resolved time-of-flight (TOF) mass spectrometry. Time-resolved TOF mass signal of parent ion (CH2ICl+) and main daughter ion (CH2Cl+) are obtained. The curve for the transient signal of CH2ICl+ is simple and can be well fitted by an exponential decay convoluted with a Gaussian function. The decay constant determined to be less than 35 fs reflects the lifetime of the B band. Significant substituent effects on photodissociation dynamics of CH2IC1 compared with CH3I are discussed. The dissociation time from the parent ion CH2IC1+ to the daughter ion CH2Cl+ is determined in the experiment. The optimized geometry of the ionic state of CH2ICl and the ionization energy are calculated for further analysis of the measurements. In addition, compared with the parent ion, a new decay component with time constant of -596 fs is observed for CH2Cl+, and reasonable mechanisms are proposed for the explanation.展开更多
AlGaN epitaxial layer has been studied by means of temperature-dependent time-integrated photoluminescence(PL)and time-resolved photoluminescence(TRPL). An enhancing redshift phenomenon in TRPL spectra with increa...AlGaN epitaxial layer has been studied by means of temperature-dependent time-integrated photoluminescence(PL)and time-resolved photoluminescence(TRPL). An enhancing redshift phenomenon in TRPL spectra with increasing temperature was observed, and the localized excitons behaved like quasi two-dimensional excitons between 6 K and 90 K. We demonstrated that these behaviors are caused by a change in the carrier dynamics with increasing temperature due to the competition of carriers' localization and delocalization in the AlGaN alloy.展开更多
Transmission measurements of warm dense iron plasma are reported over the photon energy range of 400-1200 e V, including the strong 2p-3d structures. One-dimensional hydrodynamic simulation is performed to estimate th...Transmission measurements of warm dense iron plasma are reported over the photon energy range of 400-1200 e V, including the strong 2p-3d structures. One-dimensional hydrodynamic simulation is performed to estimate the plasma conditions: temperatures of several eV and densities of about O. 1 g/cm3. By using the simulated tempera- ture and density, the calculations of the transmission spectra are performed and compared with the time-resolved experimental results.展开更多
Transient repassivation of freshly generated aluminiutn surface is monitored by using time-resolved frequency response spectroscopy. Information relating to the anodic film formation as well as the cathodic evolution ...Transient repassivation of freshly generated aluminiutn surface is monitored by using time-resolved frequency response spectroscopy. Information relating to the anodic film formation as well as the cathodic evolution of hydrogen on the metal surace is obtained as function of repassivation time and applied constant current. Pronounced effects of anodic and cathodic applied curmnts on the time-resolved impedance spectroscopy support a previous model which suggests that the low frequtncy RC time constants are due to the anodic film growth and the high Asquency one to the cathodic charpetrensfer or clectron transfer process occuwtng simultaneously on the electrode surace.展开更多
In the femtosecond laser-produced Cu-plasma, the transient transition dynamics that the excited state 5s4D7/2 via electron-ion recombination transfers to 4p4F9/20 (465.11 nm, Λ1 line) and 4p4D7/20 (529.25 nm, Λ2 ...In the femtosecond laser-produced Cu-plasma, the transient transition dynamics that the excited state 5s4D7/2 via electron-ion recombination transfers to 4p4F9/20 (465.11 nm, Λ1 line) and 4p4D7/20 (529.25 nm, Λ2 line) states are investigated by using the time-resolved spectroscopy. The occupation number and relevant lifetime of the excited state 5s4D7/2, the temporal evolutions of spectral intensities for Λ1 line and Λ2 line emissions are demonstrated to be in direct proportion to the employed laser intensity, which reveals the transient features of transition dynamics clearly differing from that resulted in the traditional collision excitation. Furthermore, some unique characteristics for Λ1 and Λ2 transitions stemming from electron-ion recombination are examined in detail.展开更多
he photoluminescence spectra of porous Si have been studied. Its timere-solved luminescence spectra show a red shift of luminescencc peak with increasingdelay time after exciting and a nonexponential decay. Several sp...he photoluminescence spectra of porous Si have been studied. Its timere-solved luminescence spectra show a red shift of luminescencc peak with increasingdelay time after exciting and a nonexponential decay. Several spectral bands withdifferent Gaussian center appear by means of the decomposition of a spetrum. Theresuits of our experiments show quantum confined characters in porous Si, and wespeculate that the short wavelength band at 465 nm is the direct band froni P_(15) toP_(25)展开更多
基金supported by the Innovation Fund for Technology Based Firms (06C26213201075)National High Technology Research and Development Program of China (863 Program) (2008AA102415)
文摘A competitive indirect time-resolved fluoroimmunoassay(TRFIA) was developed for detection of zearalenone(ZEN) in cereals,in which ZEN conjugated to bovine serum albumin(BSA) is used as solid-phase antigen.A competitive indirect TRFIA was conducted by simultaneously incubating ZEN in standard or extracted samples with anti-ZEN monoclonal antibody over ZEN-BSA coated plates,and then determining the bound ZEN monoclonal antibody with goat anti-mouse europium conjugate.Samples were extracted with methanol/water...
文摘Eu-chelate were used to construct a two-site sandwich-type assay for pepsinogen Ⅰ (PGI) with time-resolved fluoroimmunoassay (TRFIA) as a detection technique. On the noncompetitive assay, captured monoclonal antibodies (McAbs) coated on wells were directed against a specific antigenic site on the PGI. Another McAbs, called as labeling McAbs, were prepared with the Eu-chelate of N-(p-isothiocyanatobenzyl)-diethylenetriamine-N, N, N, N-tetraacetic acid and directed against a different antigenic site on the PGI. The fluorescence counts of bound Eu^3+ -McAbs were measured with the auto DELFIA1235 system. The PGI in sera from healthy volunteers were determined by PGI-TRFIA. The within-run and between-run CVs of the PGI-TRFIA were 1.9% and 4.7%, respectively, and the recovery rate was 102.65%. The assay had a detection limit of 0.05 μg· L^-1. The PGI-TRFIA provided a linear response from 3.5 to 328 μg· L^-1. The cross-reacting rate with pepsinogen Ⅱ was negligible. The linear correlation of PGI-TRFIA and radioimmunassay measurements resulted in a correlation coefficient of 0.977. The means of healthy volunteers were 154 ±43 μg·L^-1 for serum PGI. The availability of a highly sensitive, reliable, and convenient method for quantifying PGI will allow investigations into the possible diagnostic value of this analyte in various clinical conditions, including gastric carcinoma, duodenal ulcer, gastritis and severe atrophic gastritis.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.12004067,11974070,62027807,and 52272137)the National Key R&D Program of China(Grant No.2022YFA1403000)。
文摘We measure the time-resolved terahertz spectroscopy of GeSn thin film and studied the ultrafast dynamics of its photo-generated carriers.The experimental results show that there are photo-generated carriers in GeSn under femtosecond laser excitation at 2500 nm,and its pump-induced photoconductivity can be explained by the Drude–Smith model.The carrier recombination process is mainly dominated by defect-assisted Auger processes and defect capture.The firstand second-order recombination rates are obtained by the rate equation fitting,which are(2.6±1.1)×10^(-2)ps^(-1)and(6.6±1.8)×10^(-19)cm^(3)·ps^(-1),respectively.Meanwhile,we also obtain the diffusion length of photo-generated carriers in GeSn,which is about 0.4μm,and it changes with the pump delay time.These results are important for the GeSn-based infrared optoelectronic devices,and demonstrate that Ge Sn materials can be applied to high-speed optoelectronic detectors and other applications.
基金supported by the National Natural Science Foundation of China (21025729 and 20921063)
文摘A novel dual-label time-resolved fluoroimmunoassay method was developed for the simultaneous determination of chloramphenicol (CAP) and ractopamine (RAC) residues in 18 swine tissue samples,using anti-CAP and anti-RAC monoclonal antibodies labeled with europium (Eu 3+) and samarium (Sm 3+),respectively.The detection limits for CAP and RAC were 0.06 and 0.25ng/g.The recovery from swine muscle samples was 102%-121% for CAP at spiking levels of 0.1-5ng/g,and 69.8%-85.8% for RAC at spiking levels of 1-10ng/g.The results obtained from the swine tissue samples using this method showed good agreement with those obtained using ELISA and GC-MS,with correlation coefficients (R) between 0.92-0.98.
基金Project supported by the National Key Research and Development Program of China (Grant No.2017YFA0403300)the National Natural Science Foundation of China (Grant Nos.12074352 and 11675158)Fundamental Research Funds for the Central Universities in China (Grant No.YJ202144)。
文摘A study of a nanosecond laser irradiation on the titanium-layer-buried gold planar target is presented. The timeresolved x-ray emission spectra of titanium tracer are measured by a streaked crystal spectrometer. By comparing the simulated spectra obtained by using the FLYCHK code with the measured titanium spectra, the temporal plasma states, i.e.,the electron temperatures and densities, are deduced. To evaluate the feasibility of using the method for the characterization of Au plasma states, the deduced plasma states from the measured titanium spectra are compared with the Multi-1D hydrodynamic simulations of laser-produced Au plasmas. By comparing the measured and simulated results, an overall agreement for the electron temperatures is found, whereas there are deviations in the electron densities. The experiment–theory discrepancy may suggest that the plasma state could not be well reproduced by the Multi-1D hydrodynamic simulation, in which the radial gradient is not taken into account. Further investigations on the spectral characterization and hydrodynamic simulations of the plasma states are needed. All the measured and FLYCHK simulated spectra are given in this paper as datasets. The datasets are openly available at http://www.doi.org/10.57760/sciencedb.j00113.00032.
基金supported by the Scientific and Technological Research Project of Chongqing (CSTC2011ggB10009,cstc2012pt-kyys10002,cstc2012cx-rkxB10002)the National Natural Science Foundation of China(81202438)the Natural Science Foundation of the Chongqing Education Committee (KJ110605)
文摘To quantitatively determine tylosin and tilmicosin in edible animal tissues,a time-resolved fluoroimmunoassay(TRFIA) has been developed and validated.For this purpose,desmycosin-O-carboxymethoxylamine-BSA was fixed onto microtiter plates,standards and samples were loaded and,finally,diluted europium-labeled anti-tylosin antibodies were added.Results show that the limit of detection for tylosin was 0.03 ng mL-1 and that for tilmicosin was 0.05 ng mL-1.The recoveries were 73.6% to 120.5%,with coefficients of variation below 15.6% in various biological matrices spiked with tylosin and tilmicosin at concentrations of 50-200 ngg-1.There was good correlation(R2>0.99) between the TRFIA,an enzyme-linked immunosorbent assay and high performance liquid chromatography data.In conclusion,the new TRFIA is applicable to the detection of tylosin and tilmicosin and is an effective and economical method that will enable high-throughput sample screening.The method is expected to be widely applicable.
基金supported by the National Natural Science Foundation of China(No.20675063)
文摘The combined use of chemometrics and chemiluminescence(CL)measurements,with the aid of the stopped-flow mixing technique,developed a simple time-resolved CL method for the simultaneous determination of captopril(CPL)and hydrochlorothiazide(HCT).The stopped-flow technique in a continuous-flow system was employed in this work in order to emphasize the kinetic differences between the two analytes in cerium(IV)-rhodamine 6G CL system.After the flow was stopped,an initial rise of CL signal was observed for HCT standards,while a direct decay of CL signal was obtained for CPL standards.The mixed CL signal was monitored and recorded on the whole process of continuous-flow/stopped-flow,and the obtained data were processed by the chemometric approach of artificial neural network.The relative prediction error(RPE)of CPL and HCT was 5.9% and 8.7%,respectively.The recoveries of CPL and HCT in tablets were found to fall in the range between 95% and 106%.The proposed method was successfully applied to the simultaneous determination of CPL and HCT in a compound pharmaceutical formulation.
基金Project supported by the Science and Technology Development Fund Planning Project for the Universities of Tianjin,China(Grant No.20140902)the Natural Science Foundation of Tianjin City,China(Grant No.16JCQNJC01900)+1 种基金the National Natural Science Foundation of China(Grant Nos.51376136and 61474082)the Science and Technology Achievement Award Project for the Universities of Tianjin,China
文摘Aluminum ablation by multiple femtosecond laser pulses is investigated via time-resolved shadowgraphs and scanning electron microscope (SEM) images of the ablation spot. The spatial distribution of the ejected material and the radius of the shock wave generated during the ablation are found to vary with the increase in the number of pulses. In the initial two pulses, nearly concentric and semicircular stripes within the shock wave front are observed, unlike in subsequent pulses. Ablation by multiple femtosecond pulses exhibits different characteristics compared with the case induced by single femtosecond pulse because of the changes to the aluminum target surface induced by the preceding pulses.
基金Project supported by the National Natural Science Foundation of China(Nos.1332006,11272233,11202122,and 11411130150)the National Fundamental Research Program of China(973 Program)(No.2012CB720101)
文摘The relationship between the in the logarithmic law (log-law) region of bursting event and the low/high-speed streak a turbulent boundary layer is investigated. A tomographic time-resolved particle image velocimetry (TRPIV) system is used to measure the instantaneous three-dimensional-three-component (3D-3C) velocity field. The momentum thickness based Reynolds number is about 2 460. The topological information in the log-law region is obtained experimentally. It is found that the existence of the quadrupole topological structure implies a three-pair hairpin-like vortex packet, which is in connection with the low/high-speed streak. An idealized 3D topological model is then proposed to characterize the observed hairpin vortex packet and low/high-speed streak.
基金Project supported by the National Natural Science Foundation of China(Grant No.60627003)the Foundation for Creative Team in Institution of Higher Education of Guangdong Province,China(Grant No.06CXTD009)
文摘In broadband coherent anti-Stokes Raman scattering (CARS) spectroscopy with supercontinuum (SC), the simultaneously detectable spectral coverage is limited by the spectral continuity and the simultaneity of various spectral components of SC in an enough bandwidth. By numerical simulations, the optimal experimental conditions for improving the SC are obtained. The broadband time-resolved CARS spectrography based on the SC with required temporal and spectral distributions is realised. The global molecular vibrational spectrum with well suppressed nonresonant background noise can be obtained in a single measurement. At the same time, the measurements of dephasing times of various molecular vibrational modes can be conveniently achieved from intensities of a sequence of time-resolved CARS signals. It will be more helpful to provide a complete picture of molecular vibrations, and to exhibit a potential to understand not only both the solvent dynamics and the solute-solvent interactions, but also the mechanisms of chemical reactions in the fields of biology, chemistry and material science.
基金Project supported by the National Natural Science Foundation of China (Grant No 10474081).
文摘Time-resolved measurement of atomic emission enhancement is performed by using a 500-fs KrF laser pulse incident upon a high density supersonic O2 gas jet, synchronized with an orthogonal ns frequency-doubled Nd:YAG laser pulse. The ultra-short pulse serves as an igniter of the gas jet, and the subsequent ns-laser pulse significantly enhances the atomic emission. Analysis shows that the contributions to the enhancement effect are made mainly by the bremsstrahlung radiation and cascade ionization.
基金Project supported by the National Natural Science Foundation of China (Grant Nos. 20573028 and 20973050)
文摘We performed femtosecond time-resolved coherent anti-Stokes Raman scattering (fs-CARS) measurements on liquid toluene and PVK film. For both samples, we selectively excited the CH stretching vibrational modes and observed the expected quantum beat signals. The frequency of the well-defined beats is in good agreement with the energy difference between the two simultaneously excited modes, which demonstrates that a coherent coupling between the vibrational modes of the C H chemical bonds exists at the different positions of the molecules. The dephasing times of the excited modes are obtained simultaneously.
文摘Dermatophagoides farinae and D. pteronyssinus are the prevalent house dust mites (HDM) in tropical countries and are associated with allergic diseases. This investigation developed a time- resolved immunofluorometric assay (TR-IFMA) for the first time to detect specific IgE antibody in patients with skin prick test positive to HDM but no detectable IgE by other means. Levels of IgE to natural and recombinant HDM allergens were measured by TR-IFMA in 50 HDM-allergic patients and 19 healthy participants compared to sandwich enzyme-linked immunosorbent assay (ELISA). A recombinant allergen, rDerf2, showed a 14 kDa band corresponding to broad range proteins of natural HDM.TR-IFMA showed sensitivity lower than 0.35 kUA/l. TR-IFMA employing three HDM antigens showed good correlations with sandwich ELISA at R2 0.93-0.96. TR-IFMA detected HDM IgE in 62, 62, 25 percent of allergic patient serum sample compared to 28, 32, and 22 percent detected by ELISA result using three HDM allergen. TR-IFMA also detected 26.3, 31.6, and 5.3 percent positive samples from 19 healthy participants while ELISA showed 0, 5.3, and 0 percents IgE positive samples. The use of rDerf2 as an HDM allergen for the assay was verified with no statistically different from other HDM allergens. TR-IFMA showed lower detection limit than ELISA and yielded higher sensitivity for serum of people with allergic symptoms with no detectable HDM IgE. It is anticipated that TR-IFMA for HDM-specific IgE detection will play an important role in future diagnosis of HDM allergy in clinical laboratories and for different research purposes.
基金supported by National Natural Science Foundation of China (Grant No. 61505253)National Key Research and Development Plan of China (Project No. 2016YFD0200601)
文摘One of the technical bottlenecks of traditional laser-induced breakdown spectroscopy(LIBS) is the difficulty in quantitative detection caused by the matrix effect. To troubleshoot this problem,this paper investigated a combination of time-resolved LIBS and convolutional neural networks(CNNs) to improve K determination in soil. The time-resolved LIBS contained the information of both wavelength and time dimension. The spectra of wavelength dimension showed the characteristic emission lines of elements, and those of time dimension presented the plasma decay trend. The one-dimensional data of LIBS intensity from the emission line at 766.49 nm were extracted and correlated with the K concentration, showing a poor correlation of R_c^2?=?0.0967, which is caused by the matrix effect of heterogeneous soil. For the wavelength dimension, the two-dimensional data of traditional integrated LIBS were extracted and analyzed by an artificial neural network(ANN), showing R_v^2?=?0.6318 and the root mean square error of validation(RMSEV)?=?0.6234. For the time dimension, the two-dimensional data of time-decay LIBS were extracted and analyzed by ANN, showing R_v^2?=?0.7366 and RMSEV?=?0.7855.These higher determination coefficients reveal that both the non-K emission lines of wavelength dimension and the spectral decay of time dimension could assist in quantitative detection of K.However, due to limited calibration samples, the two-dimensional models presented over-fitting.The three-dimensional data of time-resolved LIBS were analyzed by CNNs, which extracted and integrated the information of both the wavelength and time dimension, showing the R_v^2?=?0.9968 and RMSEV?=?0.0785. CNN analysis of time-resolved LIBS is capable of improving the determination of K in soil.
基金Supported by the National Natural Science Foundation of China under Grant Nos 11304157,21303255 and 11475229the‘Six Talent Peaks’Project in Jiangsu Province under Grant No 2015-JNHB-011the College Students Practice Innovative Training Program of Nuist under Grant No 201610300042
文摘Ultrafast dissociation dynamics of chloroiodomethane (CH2ICl) in the B band is studied by femtosecond time- resolved time-of-flight (TOF) mass spectrometry. Time-resolved TOF mass signal of parent ion (CH2ICl+) and main daughter ion (CH2Cl+) are obtained. The curve for the transient signal of CH2ICl+ is simple and can be well fitted by an exponential decay convoluted with a Gaussian function. The decay constant determined to be less than 35 fs reflects the lifetime of the B band. Significant substituent effects on photodissociation dynamics of CH2IC1 compared with CH3I are discussed. The dissociation time from the parent ion CH2IC1+ to the daughter ion CH2Cl+ is determined in the experiment. The optimized geometry of the ionic state of CH2ICl and the ionization energy are calculated for further analysis of the measurements. In addition, compared with the parent ion, a new decay component with time constant of -596 fs is observed for CH2Cl+, and reasonable mechanisms are proposed for the explanation.
基金Project supported by the National Key Research and Development Program of China(Grant No.2016YFB0400101)Beijing Science and Technology Project,China(Grant No.Z151100003315024)
文摘AlGaN epitaxial layer has been studied by means of temperature-dependent time-integrated photoluminescence(PL)and time-resolved photoluminescence(TRPL). An enhancing redshift phenomenon in TRPL spectra with increasing temperature was observed, and the localized excitons behaved like quasi two-dimensional excitons between 6 K and 90 K. We demonstrated that these behaviors are caused by a change in the carrier dynamics with increasing temperature due to the competition of carriers' localization and delocalization in the AlGaN alloy.
基金Supported by the National Natural Science Foundation of China under Grant No 11304292the Foundation of China Academy of Engineering Physics under Grant No 2013A0102002
文摘Transmission measurements of warm dense iron plasma are reported over the photon energy range of 400-1200 e V, including the strong 2p-3d structures. One-dimensional hydrodynamic simulation is performed to estimate the plasma conditions: temperatures of several eV and densities of about O. 1 g/cm3. By using the simulated tempera- ture and density, the calculations of the transmission spectra are performed and compared with the time-resolved experimental results.
文摘Transient repassivation of freshly generated aluminiutn surface is monitored by using time-resolved frequency response spectroscopy. Information relating to the anodic film formation as well as the cathodic evolution of hydrogen on the metal surace is obtained as function of repassivation time and applied constant current. Pronounced effects of anodic and cathodic applied curmnts on the time-resolved impedance spectroscopy support a previous model which suggests that the low frequtncy RC time constants are due to the anodic film growth and the high Asquency one to the cathodic charpetrensfer or clectron transfer process occuwtng simultaneously on the electrode surace.
基金Project supported by the National Natural Science Foundation of China(Grant No.51705009)the NSAF of China(Grant No.U1530153)
文摘In the femtosecond laser-produced Cu-plasma, the transient transition dynamics that the excited state 5s4D7/2 via electron-ion recombination transfers to 4p4F9/20 (465.11 nm, Λ1 line) and 4p4D7/20 (529.25 nm, Λ2 line) states are investigated by using the time-resolved spectroscopy. The occupation number and relevant lifetime of the excited state 5s4D7/2, the temporal evolutions of spectral intensities for Λ1 line and Λ2 line emissions are demonstrated to be in direct proportion to the employed laser intensity, which reveals the transient features of transition dynamics clearly differing from that resulted in the traditional collision excitation. Furthermore, some unique characteristics for Λ1 and Λ2 transitions stemming from electron-ion recombination are examined in detail.
文摘he photoluminescence spectra of porous Si have been studied. Its timere-solved luminescence spectra show a red shift of luminescencc peak with increasingdelay time after exciting and a nonexponential decay. Several spectral bands withdifferent Gaussian center appear by means of the decomposition of a spetrum. Theresuits of our experiments show quantum confined characters in porous Si, and wespeculate that the short wavelength band at 465 nm is the direct band froni P_(15) toP_(25)