Studies on the temporal,structural,and interacting features of the clubroot resistance gene Rcr1 using CRISPR/Cas9-based systems

Clubroot disease is a severe threat to Brassica crops globally,particularly in western Canada.Genetic resistance,achieved through pyramiding clubroot resistance(CR)genes with different modes of action,is the most important strategy for managing the disease.However,studies on the CR gene functions are quite limited.In this study,we have conducted investigations into the temporal,structural,and interacting features of a newly cloned CR gene,Rcr1,using CRISPR/Cas9 technology.For temporal functionality,we developed a novel CRISPR/Cas9-based binary vector,pHHIGR-Hsp18.2,to deliver Rcr1 into a susceptible canola line(DH12075)and observed that early expression of Rcr1 is critical for conferring resistance.For structural functionality,several independent mutations in specific domains of Rcr1 resulted in loss-offunction,highlighting their importance for CR phenotype.In the study of the interacting features of Rcr1,a cysteine protease gene and its homologous allele in canola were successfully disrupted via CRISPR/Cas9 as an interacting component with Rcr1 protein,resulting in the conversion from clubroot resistant to susceptible in plants carrying intact Rcr1.These results indicated an indispensable role of these two cysteine proteases in Rcr1-mediated resistance response.This study,the first of its kind,provides valuable insights into the functionality of Rcr1.Further,the new vector p HHIGR-Hsp18.2 demonstrated an inducible feature on the removal of add-on traits,which should be useful for functional genomics and other similar research in brassica crops.

Practical Prescribed Time Tracking Control With Bounded Time-Varying Gain Under Non-Vanishing Uncertainties

This paper investigates the prescribed-time control(PTC) problem for a class of strict-feedback systems subject to non-vanishing uncertainties. The coexistence of mismatched uncertainties and non-vanishing disturbances makes PTC synthesis nontrivial. In this work, a control method that does not involve infinite time-varying gain is proposed, leading to a practical and global prescribed time tracking control solution for the strict-feedback systems, in spite of both the mismatched and nonvanishing uncertainties. Different from methods based on control switching to avoid the issue of infinite control gain that involves control discontinuity at the switching point, in our method a softening unit is exclusively included to ensure the continuity of the control action. Furthermore, in contrast to most existing prescribed-time control works where the control scheme is only valid on a finite time interval, in this work, the proposed control scheme is valid on the entire time interval. In addition, the prior information on the upper or lower bound of gi is not in need,enlarging the applicability of the proposed method. Both the theoretical analysis and numerical simulation confirm the effectiveness of the proposed control algorithm.

PD-like finite time controller with simple structure for satellite attitude control

A finite time controller with PD-like structure for satellite attitude control is proposed in this paper.The controller is constructed with simple structure based on standard PD controller.The fractional order term is designed hence system could both have strong robustness and finite time convergence rate,and the advantage of finite time control and PD control is combined in this paper.System convergence rate is discussed by Lyapunov method,and the constraint on control parameters is given by implementing the coupled term of angular velocity and attitude quaternion.Moreover,the accuracy at steady stage depending on control parameters is given hence system could converge to this field within finite time.System stability and performance is demonstrated by numerical simulation results.

Reinforcement⁃Learning⁃Based Appointed⁃Time Prescribed Performance Attitude Control for Rigid Spacecraft

This paper addresses a geometric control algorithm for the attitude tracking problem of the rigid spacecraft modeled on SO(3).Considering the topological and geometric properties of SO(3),we introduced a smooth positive attitude error function to convert the attitude tracking issue on SO(3)into the stabilization counterpart on its Lie algebra.The error transformation technique was further utilized to ensure the assigned transient and steady state performance of the attitude tracking error with the aid of a well⁃designed assigned⁃time performance function.Then,using the actor⁃critic(AC)neural architecture,an adaptive reinforcement learning approximator was constructed,in which the actor neural network(NN)was utilized to approximate the unknown nonlinearity online.A critic function was introduced to tune the next phase of the actor neural network operation for performance improvement via supervising the system performance.A rigorous stability analysis was presented to show that the assigned system performance can be achieved.Finally,the effectiveness and feasibility of the constructed control strategy was verified by the numerical simulation.

Impulsive Logistic Model for Gray Leaf Spots Caused by Cercospora zeae-maydi

[ Objectlve] Impulsive Logistic Model was used to simulate epidemic process of Gray Leaf Spots caused by C. zeae-maydi. [ Method] The pathogen was inoculated in different maize varieties, and the incidence were observed and recorded. Impulsive Logistic Model was used to simulate the development process of the disease, which was compared with actual incidence. [ Result] Artificial inoculation tests showed that impulsive Logistic Model could reflect time dynamic of C. zeae-maydi. Through derivation, exponential growth phase was from maize seedling emergence to eady July in each year, logistic phase was from early July to late August, terminal phase was from eady September to the end of maize growth stage. [ Conclusion] The derivation result from model was consistent with the development biological laws of C. zeae-maydi.

Application of Monte Carlo method in rudder control precision

After the trajectory simulation model of rudder control rocket with six degrees of freedom is established by Matlab/ Simulink, the simulated targeting of rudder control rocket with rudder angle error and starting control moment error is carried out respectively by means of Monte Carlo method and the distribution of impact points of rudder control rocket is counted from all the successful subsamples. In the case of adding interference errors associated with rudder angle error and starting time error, the simulation analysis of impact point dispersion is done and its lateral and longitudinal correction abilities at different targeting angles are simulated to identify the effects of these factors on characteristics and control precision of the rudder control rocket, which provides the relevant reference for high-precision design of rudder control system.

Iterative Learning Control for Discrete-time Stochastic Systems with Quantized Information

An iterative learning control (ILC) algorithm using quantized error information is given in this paper for both linear and nonlinear discrete-time systems with stochastic noises. A logarithmic quantizer is used to guarantee an adaptive improvement in tracking performance. A decreasing learning gain is introduced into the algorithm to suppress the effects of stochastic noises and quantization errors. The input sequence is proved to converge strictly to the optimal input under the given index. Illustrative simulations are given to verify the theoretical analysis. © 2014 Chinese Association of Automation.

Effect of Sludge Retention Time on Nitrite Accumulation in Real-time Control Biological Nitrogen Removal Sequencing Batch Reactor

In this study,four sequencing batch reactors(SBR),with the sludge retention time(SRT)of 5,10,20 and 40 d,were used to treat domestic wastewater,and the effect of SRT on nitrite accumulation in the biological nitrogen removal SBR was investigated.The real-time control strategy based on online parameters,such as pH,dissolved oxygen(DO)and oxidation reduction potential(ORP),was used to regulate the nitrite accumulation in SBR. The model-based simulation and experimental results showed that with the increase of SRT,longer time was needed to achieve high level of nitritation.In addition,the nitrite accumulation rate(NAR)was higher when the SRT was relatively shorter during a 112-day operation.When the SRT was 5 d,the system was unstable with the mixed liquor suspended solids(MLSS)decreased day after day.When the SRT was 40 d,the nitrification process was significantly inhibited.SRT of 10 to 20 d was more suitable in this study.The real-time control strategy combined with SRT control in SBR is an effective method for biological nitrogen removal via nitrite from wastewater.

Impact angle control over composite guidance law based on feedback linearization and finite time control

The impact angle control over guidance(IACG) law against stationary targets is proposed by using feedback linearization control(FLC) and finite time control(FTC). First, this paper transforms the kinematics equation of guidance systems into the feedbackable linearization model, in which the guidance law is obtained without considering the impact angle via FLC. For the purpose of the line of sight(LOS) angle and its rate converging to the desired values, the second-order LOS angle is considered as a double-integral system. Then, this paper utilizes FTC to design a controller which can guarantee the states of the double-integral system converging to the desired values. Numerical simulation illustrates the performance of the IACG, in contrast to the existing guidance law.

Seam Tracking and Visual Control for Robotic Arc Welding Based on Structured Light Stereovision

A real-time arc welding robot visual control system based on a local network with a multi-level hierarchy is developed in this paper. It consists of an intelligence and human-machine interface level, a motion planning level, a motion control level and a servo control level. The last three levels form a local real-time open robot controller, which realizes motion planning and motion control of a robot. A camera calibration method based on the relative movement of the end-effector connected to a robot is proposed and a method for tracking weld seam based on the structured light stereovision is provided. Combining the parameters of the cameras and laser plane, three groups of position values in Cartesian space are obtained for each feature point in a stripe projected on the weld seam. The accurate three-dimensional position of the edge points in the weld seam can be calculated from the obtained parameters with an information fusion algorithm. By calculating the weld seam parameter from position and image data, the movement parameters of the robot used for tracking can be determined. A swing welding experiment of type V groove weld is successfully conducted, the results of which show that the system has high resolution seam tracking in real-time, and works stably and efficiently.

Optimal Formation Reconfiguration Control of Multiple UCAVs Using Improved Particle Swarm Optimization

Optimal formation reconfiguration control of multiple Uninhabited Combat Air Vehicles （UCAVs） is a complicated global optimum problem. Particle Swarm Optimization （PSO） is a population based stochastic optimization technique inspired by social behaviour of bird flocking or fish schooling. PSO can achieve better results in a faster, cheaper way compared with other bio-inspired computational methods, and there are few parameters to adjust in PSO. In this paper, we propose an improved PSO model for solving the optimal formation reconfiguration control problem for multiple UCAVs. Firstly, the Control Parameterization and Time Diseretization （CPTD） method is designed in detail. Then, the mutation strategy and a special mutation-escape operator are adopted in the improved PSO model to make particles explore the search space more efficiently. The proposed strategy can produce a large speed value dynamically according to the variation of the speed, which makes the algorithm explore the local and global minima thoroughly at the same time. Series experimental results demonstrate the feasibility and effectiveness of the proposed method in solving the optimal formation reconfiguration control problem for multiple UCAVs.

Time delay control of hydraulic manipulators with continuous nonsingular terminal sliding mode

For the position tracking control of hydraulic manipulators,a novel method of time delay control(TDC) with continuous nonsingular terminal sliding mode(CNTSM) was proposed in this work.Complex dynamics of the hydraulic manipulator is approximately canceled by time delay estimation(TDE),which means the proposed method is model-free and no prior knowledge of the dynamics is required.Moreover,the CNTSM term with a fast-TSM-type reaching law ensures fast convergence and high-precision tracking control performance under heavy lumped uncertainties.Despite its considerable robustness against lumped uncertainties,the proposed control scheme is continuous and chattering-free and no pressure sensors are required in practical applications.Theoretical analysis and experimental results show that faster and higher-precision position tracking performance is achieved compared with the traditional CNTSM-based TDC method using boundary layers.

Weighted Time-Based Global Hierarchical Path Planning in Dynamic Environment

A weighted time-based global hierarchical path planning method is proposed to obtain the global optimal path from the starting point to the destination with time optimal control. First, the grid-or graph-based modeling is performed and the environment is divided into a set of grids or nodes. Then two time-based features of time interval and time cost are presented. The time intervals for each grid are built, during each interval the condition of the grid remains stable, and a time cost of passing through the grid is defined and assigned to each interval. Furthermore, the weight is introduced for taking both time and distance into consideration, and thus a sequence of multiscale paths with total time cost can be achieved. Experimental results show that the proposed method can handle the complex dynamic environment, obtain the global time optimal path and has the potential to be applied to the autonomous robot navigation and traffic environment.

Authentication masking code against DoS of T-MAC protocol

Security vulnerability of denial of service (DoS) in time out-medium access control (T-MAC) protocol was discussed and analysis of power consumption at each stage of T-MAC protocol was carried out. For power efficient authentication scheme which can provide reliability, efficiency, and security for a general T-MAC communication, a novel synchronization and authentication scheme using authentication masking code was proposed. Authentication data were repeated and masked by PN sequence. The simulation results show that the proposed approach can provide synchronization and authentication simultaneously for nodes in wireless sensor network (WSN). 63 bits AMC code gives above 99.97% synchronization detection and 93.98% authentication data detection probability in BER 0.031 7.

Chaos detection and control in a typical power system

In this paper, a new chaotic system is introduced. The proposed system is a conventional power network that demonstrates a chaotic behavior under special operating conditions. Some features such as Lyapunov exponents and a strange attractor show the chaotic behavior of the system, which decreases the system performance. Two different controllers are proposed to control the chaotic system. The first one is a nonlinear conventional controller that is simple and easy to construct, but the second one is developed based on the finite time control theory and optimized for faster control. A MATLAB-based simulation verifies the results.

Two-echelon inventory model with service level constraint and controllable lead time sensitive to order quantity

The decision-making and optimization of two-echelon inventory coordination were analyzed with service level constraint and controllable lead time sensitive to order quantity.First,the basic model of this problem was established and based on relevant analysis,the original model could be transformed by minimax method.Then,the optimal order quantity and production quantity influenced by service level constraint were analyzed and the boundary of optimal order quantity and production quantity was given.According to this boundary,the effective method and tactics were put forward to solve the transformed model.In case analysis,the optimal expected total cost of two-echelon inventory can be obtained and it was analyzed how service level constraint and safety factor influence the optimal expected total cost of two-echelon inventory.The results show that the optimal expected total cost of two-echelon inventory is constrained by the higher constraint between service level constraint and safety factor.

A new wide area damping controller design method considering signal transmission delay to damp interarea oscillations in power system

Wide area damping controller(WADC) is usually utilized to damp interarea low frequency oscillation in power system. However, conventional WADC design method neglects the influence of signal transmission delay and damping performance of WADC designed by the conventional method may deteriorate or even has no effect when signal transmission delay is beyond delay margin, an index that denotes delay endurance degree of power system. Therefore, a new design method for WADC under the condition of expected damping factor and required signal transmission delay is presented in this work. An improved delay margin with less conservatism is derived by adopting a new Lyapunov-Krasovskii function and more compact bounding technique on the derivative of Lyapunov-Krasovskii functional. The improved delay margin, which constructs the correlation of damping factor and signal transmission delay, can be used to design WADC. WADC designed by the proposed method can ensure that power system satisfies expected damping factor when WADC input signal is delayed within delay margin. Satisfactory test results demonstrate the effectiveness of the proposed method.

Adaptive sliding mode control of modular self-reconfigurable spacecraft with time-delay estimation

The reconstruction control of modular self-reconfigurable spacecraft (MSRS) is addressed using an adaptive sliding mode control (ASMC) scheme based on time-delay estimation (TDE) technology. In contrast to the ground, the base of the MSRS is floating when assembled in orbit, resulting in a strong dynamic coupling effect. A TED-based ASMC technique with exponential reaching law is designed to achieve high-precision coordinated control between the spacecraft base and the robotic arm. TDE technology is used by the controller to compensate for coupling terms and uncertainties, while ASMC can augment and improve TDE’s robustness. To suppress TDE errors and eliminate chattering, a new adaptive law is created to modify gain parameters online, ensuring quick dynamic response and high tracking accuracy. The Lyapunov approach shows that the tracking errors are uniformly ultimately bounded (UUB). Finally, the on-orbit assembly process of MSRS is simulated to validate the efficacy of the proposed control scheme. The simulation results show that the proposed control method can accurately complete the target module’s on-orbit assembly, with minimal perturbations to the spacecraft’s attitude. Meanwhile, it has a high level of robustness and can effectively eliminate chattering.

On String Stable Control of Platoon of Automated Vehicles with Predecessor-successor Information Framework

For the constant distance spacing policy,the existing researches of the string stability focus on the single-predecessor information framework（SPIF） and predecessor-successor information framework（PSIF）.The research results demonstrated that the string stability could not be guaranteed with the SPIF,and then the PSIF was proposed to resolve this string instability.But the issue,whether the string stability can be guaranteed when applying the PSIF,is still controversial.Meanwhile,most of the previous researches on the string stability were conducted without consideration of the parasitic time delays and lags.In this paper,the practical longitudinal vehicle dynamics model is built with consideration of the parasitic time delays and lags existing in the actuators,sensors or the communication systems.Secondly,the detailed theoretical analysis of string stability in frequency domain is conducted to demonstrate that the classical linear control laws can not ensure the string stability when applying both the symmetrical PSIF（SPSIF） and asymmetrical PSIF（APSIF）.Thirdly,a control law,which adds the position and velocity information of the leading vehicle,is proposed to guarantee string stability for small/medium platoon,and the other control law,which adds the acceleration information of the controlled vehicle,is proposed to guarantee string stability for large platoon as well as small/medium platoon.Finally,the comparative simulation is conducted to confirm the conducted analysis and the proposed control laws.The conducted research completes the means to analyze the string stability in frequency domain,provides the parameters＇ reference for the design and implementation of the practical automatic following controllers,and improves the reliability and stability of the platoon of automatic vehicles.

RESPONSE OF PARAMETRICALLY EXCITED DUFFING-VAN DER POL OSCILLATOR WITH DELAYED FEEDBACK

The dynamical behaviour of a parametrically excited Duffing-van der Pol oscillator under linear-plus-nonlinear state feedback control with a time delay is concerned. By means of the method of averaging together with truncation of Taylor expansions, two slow-flow equations on the amplitude and phase of response were derived for the case of principal parametric resonance, it is shown that the stability condition for the trivial solution is only associated with the linear terms in the original systems besides the amplitude and frequency of parametric excitation. And the trivial solution can be stabilized by appreciate choice of gains and time delay in feedback control. Different from the case of the trivial solution, the stability condition for nontrivial solutions is also associated with nonlinear terms besides linear terms in the original system. It is demonstrated that nontrivial steady state responses may lose their stability by saddle-node （SN） or Hopf bifurcation （HB） as parameters vary. The simulations, obtained by numerically integrating the original system, are in good agreement with the analytical results.