This research uses alginate and hyaluronic acid as the main component to prepare support, then explores the possibilities as a tissue engineering scaffold. Firstly,prepare HA with various average molecular weight and ...This research uses alginate and hyaluronic acid as the main component to prepare support, then explores the possibilities as a tissue engineering scaffold. Firstly,prepare HA with various average molecular weight and alginate with different viscosity,mix them up at a certain proportion and make it into a Alg Ca2 +-HA composite scaffold with a film-forming method. This article discusses the feasibility of this scaffold used in tissue engineering field according to the consequence of moisture content testing,mechanical analysis, and scanning electron microscopy analysis. The structure and properties of Alg Ca2 +-HA composite scaffold are closely related to some factors such as average molecular weight of hyaluronic acid, hyaluronic acid concentration, alginate viscosity, cross-linking agents and processing technology. The Alg Ca2+-HA composite material, which is at different proportions and adding different cross-linking agent,has some certain characteristics: moisture content ranging from 50% to 95%, tensile strength between 2.69 N/mm2 and 4.299 N/mm2, and elongation at break is about 58% to 160%.The prepared Alg Ca2+-HA composite scaffolds can be used as tissue engineering scaffolds resulting from its high moisture content, good mechanical properties and ideal pore structure.展开更多
Injectable hydrogels as an important class of biomaterials have gained much attention in tissue engineering.However,their crosslinking degree is difficult to be controlled after being injected into body.As we all know...Injectable hydrogels as an important class of biomaterials have gained much attention in tissue engineering.However,their crosslinking degree is difficult to be controlled after being injected into body.As we all know,the crosslinking degree strongly influences the physicochemical properties of hydrogels.Therefore,developing an injectable hydrogel with tunable crosslinking degree in vivo is important for tissue engineering.Herein,we present a dual crosslinking strategy to prepare injectable hydrogels with step-by-step tunable crosslinking degree using Schiff base reaction and photopolymerization.The developed hyaluronic acid/poly(y-glutamic acid)(HA/y-PGA)hydrogels exhibit step-by-step tunable swelling behavior,enzymatic degradation behavior and mechanical properties.Mechanical performance tests show that the storage moduli of HA/y-PGA hydrogels are all less than 2000 Pa and the compressive moduli are in kilopascal,which have a good match with soft tissue.In addition,NIH 3T3 cells encapsulated in HA/y-PGA hydrogel exhibit a high cell viability,indicating a good cytocompatibility of HA/y-PGA hydrogel.Therefore,the developed HA/y-PGA hydrogel as an injectable biomaterial has a good potential in soft tissue engineering.展开更多
The design and application of hyaluronic acid (HA)-based scaffolds to control cell response and construct ideal tissue engineering products have been of great interest in the past few decades. This review provides an ...The design and application of hyaluronic acid (HA)-based scaffolds to control cell response and construct ideal tissue engineering products have been of great interest in the past few decades. This review provides an overview of the biological properties of HA to better understand how to engineer a cell-scaffold composite that is qualified in tissue engineering;important tissue engineering applications of HA including cartilage tissue engineering;and lastly, the problems of the current research on HA. All the data described above were collected and analyzed from PubMed, EMBASE and Medline.展开更多
A hyaluronic acid granular hydrogel can promote neuronal and astrocyte colony formation and axonal extension in vitro,suggesting that the hydrogel can simulate an extracellular matrix structure to promote neural regen...A hyaluronic acid granular hydrogel can promote neuronal and astrocyte colony formation and axonal extension in vitro,suggesting that the hydrogel can simulate an extracellular matrix structure to promote neural regeneration.However,in vivo experiments have not been conducted.In this study,we transplanted a hyaluronic acid granular hydrogel nerve guidance conduit to repair a 10-mm long sciatic nerve gap.The Basso,Beattie,and Bresnahan locomotor rating scale,sciatic nerve compound muscle action potential recording,Fluoro-Gold retrograde tracing,growth related protein 43/S100 immunofluorescence staining,transmission electron microscopy,gastrocnemius muscle dry/wet weight ratio,and Masson’s trichrome staining results showed that the nerve guidance conduit exhibited similar regeneration of sciatic nerve axons and myelin sheath,and recovery of the electrophysiological function and motor function as autologous nerve transplantation.The conduit results were superior to those of a bulk hydrogel or silicone tube transplant.These findings suggest that tissue-engineered nerve conduits containing hyaluronic acid granular hydrogels effectively promote the morphological and functional recovery of the injured sciatic nerve.The nerve conduits have the potential as a material for repairing peripheral nerve defects.展开更多
Injury in the central nervous system (CNS), stroke in the brain and trauma in the spinal cord in particular, may result in permanent disability of the patients, since CNS axons do not regenerate appreciably in their n...Injury in the central nervous system (CNS), stroke in the brain and trauma in the spinal cord in particular, may result in permanent disability of the patients, since CNS axons do not regenerate appreciably in their native environment due to several inhibitory molecules in the extracellular environment. Therefore, no effective clinical therapies so far are convincingly accepted for CNS injuries. Tissue engineering strategies employing biomaterials are now considered as a promising approach for restoration of these injuries, and hydrogel-based biomaterials are widely employed in this field. Among them, many studies have proven that hyaluronic acid (HA) hydrogel is a reliable and effective biomaterial, which can be a well compatible scaffold with CNS tissue and creating a good microenvironment of neural regeneration in the CNS tissue. The aim of this review is to outline how to use HA-based scaffolds to build up a suitable microenvironment of neural regeneration and restoration after CNS injury, and thereby to indicate the HA hydrogel is a promising scaffold for restoration in the CNS.展开更多
Hyaluronic acid is a naturally occurring biopolymer possessing numerous functions within the body including wound repair,cell migration,and cell signaling.Due to its versatility,hyaluronic acid has been a major compon...Hyaluronic acid is a naturally occurring biopolymer possessing numerous functions within the body including wound repair,cell migration,and cell signaling.Due to its versatility,hyaluronic acid has been a major component of biomedical research and has seen application in several fields such as tissue engineering and cancer treatments through a multitude of different forms.This review aims to outline hyaluronic acid’s role in the healing process while focusing on its myriad of forms and applications in the biomedical world.展开更多
目的:探讨纳米珍珠粉人工骨在兔股骨远端骨缺损中的修复能力。方法:将32只新西兰大白兔随机分为纳米珍珠粉/重组人骨形态发生蛋白2(recombinant human bone morphogenetic protein 2,rhBMP-2)/透明质酸人工骨组、纳米珍珠粉/透明质酸人...目的:探讨纳米珍珠粉人工骨在兔股骨远端骨缺损中的修复能力。方法:将32只新西兰大白兔随机分为纳米珍珠粉/重组人骨形态发生蛋白2(recombinant human bone morphogenetic protein 2,rhBMP-2)/透明质酸人工骨组、纳米珍珠粉/透明质酸人工骨组、单纯珍珠粉组和空白对照组,每组8只。在兔股骨远端干骺线处制备直径为7 mm,高为10 mm的圆柱状骨缺损模型,按分组植入不同骨替代材料,通过大体标本、影像学检查、组织病理学检查来观察不同材料的成骨修复效果。结果:影像学检查表明:纳米珍珠粉/rhBMP-2/透明质酸人工骨组的骨修复效果优于单纯珍珠粉组和纳米珍珠粉/透明质酸人工骨组,3个实验组的骨修复效果均优于未放置任何骨替代材料的空白对照组;组织学结果表明:在骨缺损造模术后第4周、8周、12周,纳米珍珠粉/rhBMP-2/透明质酸人工骨组的骨修复速度要比单纯珍珠粉组和纳米珍珠粉/透明质酸人工骨组快,空白对照组的骨修复速度远落后于3个实验组。骨钙素抗体染色结果表明:纳米珍珠粉/rhBMP-2/透明质酸人工骨组的成骨效果优于纳米珍珠粉/透明质酸人工骨组(P<0.05);纳米珍珠粉/透明质酸人工骨组与单纯珍珠粉组的成骨效果相比差异不明显(P>0.05);单纯珍珠粉组和空白对照组之间成骨效果差异明显(P<0.05)。Ⅰ型胶原蛋白抗体的染色结果表明:纳米珍珠粉/rhBMP-2/透明质酸人工骨组的成骨效果与纳米珍珠粉/透明质酸人工骨组相比差异不明显(P>0.05),纳米珍珠粉/透明质酸人工骨组的成骨效果优于单纯珍珠粉组和空白对照组(均P<0.05)。结论:纳米珍珠粉及其人工骨能够促进骨缺损的修复,而其中含有rhBMP-2的纳米珍珠粉人工骨对骨缺损的成骨修复效果更佳。展开更多
文摘This research uses alginate and hyaluronic acid as the main component to prepare support, then explores the possibilities as a tissue engineering scaffold. Firstly,prepare HA with various average molecular weight and alginate with different viscosity,mix them up at a certain proportion and make it into a Alg Ca2 +-HA composite scaffold with a film-forming method. This article discusses the feasibility of this scaffold used in tissue engineering field according to the consequence of moisture content testing,mechanical analysis, and scanning electron microscopy analysis. The structure and properties of Alg Ca2 +-HA composite scaffold are closely related to some factors such as average molecular weight of hyaluronic acid, hyaluronic acid concentration, alginate viscosity, cross-linking agents and processing technology. The Alg Ca2+-HA composite material, which is at different proportions and adding different cross-linking agent,has some certain characteristics: moisture content ranging from 50% to 95%, tensile strength between 2.69 N/mm2 and 4.299 N/mm2, and elongation at break is about 58% to 160%.The prepared Alg Ca2+-HA composite scaffolds can be used as tissue engineering scaffolds resulting from its high moisture content, good mechanical properties and ideal pore structure.
基金the National Natural Science Foundation of China(No.31771049)Foundation of key R&D Project of Jiangsu Province(No.BE2018731)+1 种基金Research Foundation of State Key Laboratory of Materials-Oriented Chemical Engineering(Nos.ZK201806 and No.KLI 8-06)the Six Talent Peaks Project of Jiangsu Province(No.SWYY-046)。
文摘Injectable hydrogels as an important class of biomaterials have gained much attention in tissue engineering.However,their crosslinking degree is difficult to be controlled after being injected into body.As we all know,the crosslinking degree strongly influences the physicochemical properties of hydrogels.Therefore,developing an injectable hydrogel with tunable crosslinking degree in vivo is important for tissue engineering.Herein,we present a dual crosslinking strategy to prepare injectable hydrogels with step-by-step tunable crosslinking degree using Schiff base reaction and photopolymerization.The developed hyaluronic acid/poly(y-glutamic acid)(HA/y-PGA)hydrogels exhibit step-by-step tunable swelling behavior,enzymatic degradation behavior and mechanical properties.Mechanical performance tests show that the storage moduli of HA/y-PGA hydrogels are all less than 2000 Pa and the compressive moduli are in kilopascal,which have a good match with soft tissue.In addition,NIH 3T3 cells encapsulated in HA/y-PGA hydrogel exhibit a high cell viability,indicating a good cytocompatibility of HA/y-PGA hydrogel.Therefore,the developed HA/y-PGA hydrogel as an injectable biomaterial has a good potential in soft tissue engineering.
文摘The design and application of hyaluronic acid (HA)-based scaffolds to control cell response and construct ideal tissue engineering products have been of great interest in the past few decades. This review provides an overview of the biological properties of HA to better understand how to engineer a cell-scaffold composite that is qualified in tissue engineering;important tissue engineering applications of HA including cartilage tissue engineering;and lastly, the problems of the current research on HA. All the data described above were collected and analyzed from PubMed, EMBASE and Medline.
基金supported by the National Natural Science Foundation of China,Nos.81620108008(to YQL),31971112(to YQL),82071373(to JC)Innovation Capability Support Program of Shaanxi,No.2021TD-57(to YQL)。
文摘A hyaluronic acid granular hydrogel can promote neuronal and astrocyte colony formation and axonal extension in vitro,suggesting that the hydrogel can simulate an extracellular matrix structure to promote neural regeneration.However,in vivo experiments have not been conducted.In this study,we transplanted a hyaluronic acid granular hydrogel nerve guidance conduit to repair a 10-mm long sciatic nerve gap.The Basso,Beattie,and Bresnahan locomotor rating scale,sciatic nerve compound muscle action potential recording,Fluoro-Gold retrograde tracing,growth related protein 43/S100 immunofluorescence staining,transmission electron microscopy,gastrocnemius muscle dry/wet weight ratio,and Masson’s trichrome staining results showed that the nerve guidance conduit exhibited similar regeneration of sciatic nerve axons and myelin sheath,and recovery of the electrophysiological function and motor function as autologous nerve transplantation.The conduit results were superior to those of a bulk hydrogel or silicone tube transplant.These findings suggest that tissue-engineered nerve conduits containing hyaluronic acid granular hydrogels effectively promote the morphological and functional recovery of the injured sciatic nerve.The nerve conduits have the potential as a material for repairing peripheral nerve defects.
文摘Injury in the central nervous system (CNS), stroke in the brain and trauma in the spinal cord in particular, may result in permanent disability of the patients, since CNS axons do not regenerate appreciably in their native environment due to several inhibitory molecules in the extracellular environment. Therefore, no effective clinical therapies so far are convincingly accepted for CNS injuries. Tissue engineering strategies employing biomaterials are now considered as a promising approach for restoration of these injuries, and hydrogel-based biomaterials are widely employed in this field. Among them, many studies have proven that hyaluronic acid (HA) hydrogel is a reliable and effective biomaterial, which can be a well compatible scaffold with CNS tissue and creating a good microenvironment of neural regeneration in the CNS tissue. The aim of this review is to outline how to use HA-based scaffolds to build up a suitable microenvironment of neural regeneration and restoration after CNS injury, and thereby to indicate the HA hydrogel is a promising scaffold for restoration in the CNS.
文摘Hyaluronic acid is a naturally occurring biopolymer possessing numerous functions within the body including wound repair,cell migration,and cell signaling.Due to its versatility,hyaluronic acid has been a major component of biomedical research and has seen application in several fields such as tissue engineering and cancer treatments through a multitude of different forms.This review aims to outline hyaluronic acid’s role in the healing process while focusing on its myriad of forms and applications in the biomedical world.
文摘目的:探讨纳米珍珠粉人工骨在兔股骨远端骨缺损中的修复能力。方法:将32只新西兰大白兔随机分为纳米珍珠粉/重组人骨形态发生蛋白2(recombinant human bone morphogenetic protein 2,rhBMP-2)/透明质酸人工骨组、纳米珍珠粉/透明质酸人工骨组、单纯珍珠粉组和空白对照组,每组8只。在兔股骨远端干骺线处制备直径为7 mm,高为10 mm的圆柱状骨缺损模型,按分组植入不同骨替代材料,通过大体标本、影像学检查、组织病理学检查来观察不同材料的成骨修复效果。结果:影像学检查表明:纳米珍珠粉/rhBMP-2/透明质酸人工骨组的骨修复效果优于单纯珍珠粉组和纳米珍珠粉/透明质酸人工骨组,3个实验组的骨修复效果均优于未放置任何骨替代材料的空白对照组;组织学结果表明:在骨缺损造模术后第4周、8周、12周,纳米珍珠粉/rhBMP-2/透明质酸人工骨组的骨修复速度要比单纯珍珠粉组和纳米珍珠粉/透明质酸人工骨组快,空白对照组的骨修复速度远落后于3个实验组。骨钙素抗体染色结果表明:纳米珍珠粉/rhBMP-2/透明质酸人工骨组的成骨效果优于纳米珍珠粉/透明质酸人工骨组(P<0.05);纳米珍珠粉/透明质酸人工骨组与单纯珍珠粉组的成骨效果相比差异不明显(P>0.05);单纯珍珠粉组和空白对照组之间成骨效果差异明显(P<0.05)。Ⅰ型胶原蛋白抗体的染色结果表明:纳米珍珠粉/rhBMP-2/透明质酸人工骨组的成骨效果与纳米珍珠粉/透明质酸人工骨组相比差异不明显(P>0.05),纳米珍珠粉/透明质酸人工骨组的成骨效果优于单纯珍珠粉组和空白对照组(均P<0.05)。结论:纳米珍珠粉及其人工骨能够促进骨缺损的修复,而其中含有rhBMP-2的纳米珍珠粉人工骨对骨缺损的成骨修复效果更佳。
