Objective: To analyze the relationship between the numbers of cases experienced and the operation time for a single surgeon aiming to master the TLH surgical technique. Material and Methods: Retrospective data analysi...Objective: To analyze the relationship between the numbers of cases experienced and the operation time for a single surgeon aiming to master the TLH surgical technique. Material and Methods: Retrospective data analysis of women who underwent TLH for benign diseases between April, 2014 and March, 2016 was conducted by a single surgeon in a single hospital (Showa University of Fujigaoka Hospital). We divided the main procedures of the TLH operation into five sections, and measured the time required for each section. These cases were divided into three groups, group 1, 2, and 3. Results: There were 54 cases of TLH over two years for a single surgeon, and 21 cases that included essential operative procedures were divided into three groups of seven cases each. The average duration of the surgery (min.) was 178.3 ± 48.2 in the group 1, 128.3 ± 15.6 in the group 2, and 111.3 ± 15.9 in the group 3. A significant reduction in the required time was observed between group 1, 2, and 3 groups. As the number of cases increased, the operation time became statistically significantly shorter for every section except B and D. The skill growth rate was different at each section. Conclusion: For a single surgeon, as the number of surgical cases increased, we recognized the increased skill with the procedure in every section and the rate of skill growth differed for different sections. The difference of growth rate for each section implied that the number of operative cases required for a surgeon in each section was different.展开更多
目的:对副溶血弧菌(Vibrio parahaem olyticus,Vp)临床分离株中不耐热溶血素(thermolab ile hemolysin,TLH)基因进行检测,评价TLH基因在临床检测中的价值,为副溶血弧菌快速检测和新国标方法的建立打下基础。方法:用PCR方法对副溶血弧菌...目的:对副溶血弧菌(Vibrio parahaem olyticus,Vp)临床分离株中不耐热溶血素(thermolab ile hemolysin,TLH)基因进行检测,评价TLH基因在临床检测中的价值,为副溶血弧菌快速检测和新国标方法的建立打下基础。方法:用PCR方法对副溶血弧菌临床分离株的不耐热溶血素(TLH)溶血素基因进行检测。结果:所有副溶血弧菌菌株均可检测到TLH基因。结论:TLH基因在副溶血弧菌的检测中有一定的价值。展开更多
利用DNA环介导恒温核酸扩增法(loop-mediated isothermal amplification of DNA,LAMP)设计一对外引物和一对内引物,通过引物特异性识别tlh基因上的六个独立区域来快速检测副溶血弧菌。同时将检测结果与PCR方法进行比较。结果表明,LAMP...利用DNA环介导恒温核酸扩增法(loop-mediated isothermal amplification of DNA,LAMP)设计一对外引物和一对内引物,通过引物特异性识别tlh基因上的六个独立区域来快速检测副溶血弧菌。同时将检测结果与PCR方法进行比较。结果表明,LAMP反应在65℃恒温条件60min内完成,凝胶电泳呈现梯型条带;肉眼观察阳性结果出现白色混浊现象,添加1×SYBRGreenI荧光染料后,绿色的阳性结果很明显区别于橙色阴性结果;LAMP方法的最低检出限为10CFU/ml,PCR方法为103CFU/ml,LAMP方法检测灵敏度是PCR方法的100倍。因此,LAMP方法用于快速检测副溶血弧菌具有检测过程简单、反应结果肉眼即辨别并且灵敏度高特异性强的特点。实验装置简便,能够提供稳定热源即可,所以LAMP方法特别适合于现场快速诊断。展开更多
文摘Objective: To analyze the relationship between the numbers of cases experienced and the operation time for a single surgeon aiming to master the TLH surgical technique. Material and Methods: Retrospective data analysis of women who underwent TLH for benign diseases between April, 2014 and March, 2016 was conducted by a single surgeon in a single hospital (Showa University of Fujigaoka Hospital). We divided the main procedures of the TLH operation into five sections, and measured the time required for each section. These cases were divided into three groups, group 1, 2, and 3. Results: There were 54 cases of TLH over two years for a single surgeon, and 21 cases that included essential operative procedures were divided into three groups of seven cases each. The average duration of the surgery (min.) was 178.3 ± 48.2 in the group 1, 128.3 ± 15.6 in the group 2, and 111.3 ± 15.9 in the group 3. A significant reduction in the required time was observed between group 1, 2, and 3 groups. As the number of cases increased, the operation time became statistically significantly shorter for every section except B and D. The skill growth rate was different at each section. Conclusion: For a single surgeon, as the number of surgical cases increased, we recognized the increased skill with the procedure in every section and the rate of skill growth differed for different sections. The difference of growth rate for each section implied that the number of operative cases required for a surgeon in each section was different.
文摘目的:对副溶血弧菌(Vibrio parahaem olyticus,Vp)临床分离株中不耐热溶血素(thermolab ile hemolysin,TLH)基因进行检测,评价TLH基因在临床检测中的价值,为副溶血弧菌快速检测和新国标方法的建立打下基础。方法:用PCR方法对副溶血弧菌临床分离株的不耐热溶血素(TLH)溶血素基因进行检测。结果:所有副溶血弧菌菌株均可检测到TLH基因。结论:TLH基因在副溶血弧菌的检测中有一定的价值。
文摘利用DNA环介导恒温核酸扩增法(loop-mediated isothermal amplification of DNA,LAMP)设计一对外引物和一对内引物,通过引物特异性识别tlh基因上的六个独立区域来快速检测副溶血弧菌。同时将检测结果与PCR方法进行比较。结果表明,LAMP反应在65℃恒温条件60min内完成,凝胶电泳呈现梯型条带;肉眼观察阳性结果出现白色混浊现象,添加1×SYBRGreenI荧光染料后,绿色的阳性结果很明显区别于橙色阴性结果;LAMP方法的最低检出限为10CFU/ml,PCR方法为103CFU/ml,LAMP方法检测灵敏度是PCR方法的100倍。因此,LAMP方法用于快速检测副溶血弧菌具有检测过程简单、反应结果肉眼即辨别并且灵敏度高特异性强的特点。实验装置简便,能够提供稳定热源即可,所以LAMP方法特别适合于现场快速诊断。
