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Mindfulness,Smile,Choice,Practice:Thoughts Based on Improving the Mental Health of Adolescent Secondary Vocational Students
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作者 Jianfang Zhu 《Journal of Contemporary Educational Research》 2023年第11期24-32,共9页
Through the results of observations,questionnaires,tests,and other aspects from students,parents,teachers,schools,and society,we deeply explored the reality in the current education process of secondary vocational stu... Through the results of observations,questionnaires,tests,and other aspects from students,parents,teachers,schools,and society,we deeply explored the reality in the current education process of secondary vocational students,and actively thought about how to improve the current reality.We also explored the views and concepts more suitable for current students and times,created better educational and teaching activities to enhance students’perception and experience,so that students can truly enjoy the process,accumulate psychological resources and energy,and successfully complete the tasks of adolescent personality identity and development.Students can follow the self-education path of mindfulness,smile,choice,and practice,truly improve the level of mental health,and lay a solid foundation for becoming high-quality and highly-skilled talents. 展开更多
关键词 Morality and people cultivation Mental health Psychological experience mindFULNEss sELF-EDUCATION
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Research on the Cultivation of Craftsmanship in Huang Yanpei’s Education Thought
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作者 Shang Wang Fei Peng Jie Qiu 《Journal of Contemporary Educational Research》 2023年第3期14-19,共6页
Skilled talents are the main support for the strategic development of science and technology in China.The quality of talents is an important guarantee for the transformation and upgrading of national industries.By ana... Skilled talents are the main support for the strategic development of science and technology in China.The quality of talents is an important guarantee for the transformation and upgrading of national industries.By analyzing and studying Huang Yanpei’s thought on vocational education,this paper focuses on the cultivation of students’craftsmanship in vocational schools.First,we analyze the results of previous research and then elaborate on the connotations of Huang Yanpei’s education thought and craftsmanship spirit.Lastly,we propose five suggestions for the cultivation of craftsmanship.Our research work carries certain reference value to the study of craftsmanship cultivation in Huang Yanpei’s education thought. 展开更多
关键词 cultivation of craftsmanship Craftsmanship TEACHING Huang Yanpei’s education thought
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U-G-S协同推进创新人才早期培养的国际经验与启示
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作者 陆超 金辉召 黄雪君 《教育参考》 2024年第6期43-52,84,共11页
本文选取美国、澳大利亚、新加坡等创新人才机制相对成熟的国家,探究其创新人才多主体早期培育项目经验,分析、归纳得出主要培养路径有四:首先,“丰富和加速”是主要培养手段,以高阶课程助力超前学习、以缩短学制促进加速学习、以暑期... 本文选取美国、澳大利亚、新加坡等创新人才机制相对成熟的国家,探究其创新人才多主体早期培育项目经验,分析、归纳得出主要培养路径有四:首先,“丰富和加速”是主要培养手段,以高阶课程助力超前学习、以缩短学制促进加速学习、以暑期项目丰富课程学习;其次,以信息技术搭建资优教育平台,扩展资源网络渠道,推进创新人才培养的机会均等;再次,注重教育体系衔接,以大中小衔接体系为创新人才接续培养枢纽,确保培养质量;最后,以分类培养理念创新人才和技术创新人才为目标,建立针对性培养体系。据此,本文提出改进我国创新人才早期培养U-G-S协作机制的建议:加强政策顶层设计、扶持校企联合、增强创新人才网络资源建设、推进校本创新人才培养项目建设等。 展开更多
关键词 创新人才 早期培养 U-G-s
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“优师计划”背景下师范生U-G-S培养模式价值、问题及对策
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作者 尹婷婷 蒋丽 高鹏飞 《唐山师范学院学报》 2024年第3期106-109,共4页
从“优师计划”背景下师范生U-G-S培养模式价值分析入手,剖析“优师计划”背景下师范生U-G-S培养模式中地方政府、师范院校、中小学校、师范生四个基本要素各自存在问题,提出“四位一体”师范生U-G-S培养模式提升对策,以当地政府为主导... 从“优师计划”背景下师范生U-G-S培养模式价值分析入手,剖析“优师计划”背景下师范生U-G-S培养模式中地方政府、师范院校、中小学校、师范生四个基本要素各自存在问题,提出“四位一体”师范生U-G-S培养模式提升对策,以当地政府为主导、以师范院校为引领、以中小学校为核心、以师范生为主体,统筹各方培养资源和优势力量,激活多方利益主体意愿,助力乡村卓越教师的培养。 展开更多
关键词 “优师计划” 师范生 U-G-s培养模式
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Research on the Influence of Family Education on Children’s Behavioral Habits
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作者 Jiayi Wen 《Journal of Contemporary Educational Research》 2024年第8期176-180,共5页
Under the background of the all-round deepening of quality education,the cultivation of comprehensive quality has become the main theme of contemporary education reform.Good behavior and habits are of great significan... Under the background of the all-round deepening of quality education,the cultivation of comprehensive quality has become the main theme of contemporary education reform.Good behavior and habits are of great significance to children’s future learning,growth,and development.Through literature review and other methods,this paper analyzes the current situation of children’s family education and the influence of family education on the cultivation of children’s behavioral habits and provides some strategies for cultivating children’s good behavioral habits in family education. 展开更多
关键词 Family education Children’s behavior Habit cultivation PARENTs
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China's 15-year-old Postdoc System Cultivates 13,100 High-level Professionals
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《Bulletin of the Chinese Academy of Sciences》 2000年第4期198-198,共1页
Since its founding in 1985, China’s Post-doc system has cultivated a total of 13,100 high-level professionals, a national conference of postdocs revealed recently. The program’s establishment was given the green lig... Since its founding in 1985, China’s Post-doc system has cultivated a total of 13,100 high-level professionals, a national conference of postdocs revealed recently. The program’s establishment was given the green light by Deng Xiaoping in 1985 in response to a suggestion by Prof. Tsung Dao Lee, a Nobel physics prize laureate. Starting from scratch, so far 798 programs for postdocs have been established, to be distributed in 展开更多
关键词 CAs Pr China’s 15-year-old Postdoc system cultivates 13 100 High-level Professionals
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成效·局限·改革:“G-U-S”三位一体协同育师机制审思 被引量:3
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作者 徐红 《河北师范大学学报(教育科学版)》 北大核心 2023年第1期110-115,共6页
“G-U-S”三位一体协同育师机制是指,政府、高校、中小学(含幼儿园)三方各司其职,责任共担、协同培育教师的一种制度形式与工作方式。现有的三位一体协同育师机制尽管促进了教师培育成效提高、教师教育资源整合、区域教育均衡发展及教... “G-U-S”三位一体协同育师机制是指,政府、高校、中小学(含幼儿园)三方各司其职,责任共担、协同培育教师的一种制度形式与工作方式。现有的三位一体协同育师机制尽管促进了教师培育成效提高、教师教育资源整合、区域教育均衡发展及教师专业水平持续提升,但仍然存在相关决策主体单一、三元主体权责不明、培育方案不够成熟、协同培育管理不够协调等局限。为进一步提升“三位一体”协同育师机制的成效,理应构建具有一定行政职能的协同育师主体联盟,明晰“三位”主体各自的权责,完善协同育师培育方案,促进协同培育管理更加协调。 展开更多
关键词 G-U-s模式 U-G-s模式 三位一体协同育师机制 教师教育
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Analysis of cultivable aerobic bacterial community composition and screening for facultative sulfate-reducing bacteria in marine corrosive steel 被引量:4
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作者 LI Xiaohong XIAO Hui +7 位作者 ZHANG Wenjun LI Yongqian TANG Xuexi DUAN Jizhou YANG Zhibo WANG Jing GUAN Fang DING Guoqing 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2019年第2期600-614,共15页
Anaerobic, aerobic, and facultative bacteria are all present in corrosive environments. However, as previous studies to address corrosion in the marine environment have largely focused on anaerobic bacteria, limited a... Anaerobic, aerobic, and facultative bacteria are all present in corrosive environments. However, as previous studies to address corrosion in the marine environment have largely focused on anaerobic bacteria, limited attention has been paid to the composition and function of aerobic and facultative bacteria in this process. For analysis in this study, ten samples were collected from rust layers on steel plates that had been immersed in seawater for diff erent periods (i.e., six months and eight years) at Sanya and Xiamen, China. The cultivable aerobic bacterial community structure as well as the number of sulfate-reducing bacteria (SRB) were analyzed in both cases, while the proportion of facultative SRB among the isolated aerobic bacteria in each sample was also evaluated using a novel approach. Bacterial abundance results show that the proportions are related to sea location and immersion time;abundances of culturable aerobic bacteria (CAB) and SRB from Sanya were greater in most corrosion samples than those from Xiamen, and abundances of both bacterial groups were greater in samples immersed for six months than for eight years. A total of 213 isolates were obtained from all samples in terms of CAB community composition, and a phylogenetic analysis revealed that the taxa comprised four phyla and 31 genera. Bacterial species composition is related to marine location;the results show that Firmicutes and Proteobacteria were the dominant phyla, accounting for 98.13% of the total, while Bacillus and Vibrio were the dominant genera, accounting for 53.06% of the total. An additional sixfacultative SRB strains were also screened from the isolates obtained and were found to encompass the genus Vibrio (four strains), Staphylococcus (one strain), and Photobacterium (one strain). It is noteworthy that mentions of Photobacterium species have so far been absent from the literature, both in terms of its membership of the SRB group and its relationship to corrosion. 展开更多
关键词 MARINE corrosive sTEEL cultivable AEROBIC BACTERIA FACULTATIVE sulfate-reducing BACTERIA bacterial community composition 16s RRNA gene sequencing
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Eco-mindfulness in Buddhism
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作者 Kenneth Lee 《Journal of Literature and Art Studies》 2017年第12期1645-1650,共6页
Many of those involved in the ecological movement have found inspiration and parallels within the Buddhist tradition. The Buddhist philosophy of karmic causality and dependent origination (pratitya-samutpada) convey... Many of those involved in the ecological movement have found inspiration and parallels within the Buddhist tradition. The Buddhist philosophy of karmic causality and dependent origination (pratitya-samutpada) convey the notion of interdependence of humans and nature. Today, especially in the United States, there are many movements, such as the Buddhist Peace Fellowship, to promote eco-living and sustainable communities, which call for humans to be mindful of the environment and make conscientious choices for the benefit of all sentient beings and non-sentient entities. Just as humans have been the cause of the gradual deterioration of Nature, we can also be the solution towards an coo-conscious living, which not only fosters a healthy environment, but also enables us to build good merit. The core Buddhist teaching, which must be embraced by all eco-minded practitioners is the notion of interdependence, which has been expounded by notable Japanese Buddhist figures in the eighth century, such as Kukai (774-835) of the Shingon School and Dogen (1200-1253) of the Soto Zen sect, who both viewed that non-living life forms--trees, plants, and the earth--could also achieve enlightenment. Their view is based on the ontological notions of Buddha-nature (tathagata-garbha) and Indra's Jewel Net, which is a powerful image found in Huayen's (Jpn. Kegon) Avatamsaka Sutra. This paper examines Buddhist teachings and sutras, which support eco-friendly and sustainable movements in our society and world today. 展开更多
关键词 mindFULNEss BUDDHIsM nature environment dependent origination Eco-living Indra's Net
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The state of the Mind in a Revolutionary state of the State
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作者 Nditsheni Emmanuel Tshikwatamba 《Sociology Study》 2014年第1期69-84,共16页
The article epitomises its foundations by providing definitional and explanative dichotomy among the State, Nation, and Nation State. It affords a depiction of the centrality of the mind, the interface of the body and... The article epitomises its foundations by providing definitional and explanative dichotomy among the State, Nation, and Nation State. It affords a depiction of the centrality of the mind, the interface of the body and mind in behavioural manifestations. It furthermore acknowledges that nothing occurs in a vacuum but in the context of the fullness of the mind. The visible manifestations of human behaviour are contended to be informed by the noiselessness invisible aspects of the mind. The same noiseless mind that informs the behavioural visibility that becomes somewhat active and busier in its conspicuousness. The dual existence of things and events in a revolutionary context that transcends to other behavioural undertones is discoursed. In the context application of the revolutionary mapping, the mind is presented as a culprit revolutionarist that wages revolutions without fear of external threats through arrests, danger, pain, terror, dread, apprehension, detentions, and any other similar means including death. The article settles that the mind is the determinant factor of human behaviours and it therefore defines the state of the State in any revolutionary circumstance. It denotes a functional separatism of the mind from the brain which is an organ of the body thereby equally accepting that the brain functionalises the mind. The equal essential role of the mind in post revolution construction to establish and promote an acceptable system of government that responds to socio-economic factors is detailed herein the manuscript. A deliberate attempt is made not to be suggestive and prescriptive on the shape and form of such a post revolution system of government. 展开更多
关键词 Revolutionary mind DUALIsM state(s CONsTRUCTs
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A Review on the Evolution and Development of Searle' s Philosophy of Mind
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作者 Zhao Yingji 《学术界》 CSSCI 北大核心 2013年第12期291-299,共9页
The evolution and development of Searle’s philosophy of mind can be divided into two important stages,i.e.the origin and beginning stage between 1960sand 1970s,the formation and development stage since 1980s.Searle’... The evolution and development of Searle’s philosophy of mind can be divided into two important stages,i.e.the origin and beginning stage between 1960sand 1970s,the formation and development stage since 1980s.Searle’s Speech Act Theory started the research on philosophy of mind,which is considered as the origin of his philosophy of mind.The Theories of Intentionality and Chinese Room Argument are the most important and most influential academic findings in his research in the field of philosophy of mind,which lay a solid theoretical foundation for the formation and development of his theories of philosophy of mind.Searle’s philosophy of mind finds wide prospect in the research field,which provides a new direction to understand the world and probe into mind,proposes a new perspective and methodology for the study of language,and promotes the new development of the study of language. 展开更多
关键词 心灵哲学 塞尔 述评 演进 心境 言语行为理论 学术成果 意向性
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Nature's Influence on Children: Wordsworth's Concern with Children's Mind
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作者 王晓东 《海外英语》 2010年第8X期205-208,共4页
Romanticism is an important stage in the evolution of man's self-consciousness. It shows new interest in and new concern with man and nature. Wordsworth is well-known as a nature poet. The theme of human mind and ... Romanticism is an important stage in the evolution of man's self-consciousness. It shows new interest in and new concern with man and nature. Wordsworth is well-known as a nature poet. The theme of human mind and nature in his poems has long attracted readers' attention. However, he does not indulge in nature itself but takes it as consolation and inspiration for human kind. In order to highlight the poet's concern with human mind, the present paper chooses to explore Wordsworth's concern with children's mind, based on the analysis of Wordsworth's The Prelude and many of his poems about children. 展开更多
关键词 ROMANTICIsM WORDsWORTH children’s mind
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基于CLUE-S情景模拟的挠力河流域耕地适宜性评价研究 被引量:1
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作者 李衡 陈国银 +3 位作者 王世晶 张震 张志伟 靳焕焕 《农业机械学报》 EI CAS CSCD 北大核心 2023年第10期329-339,共11页
以挠力河流域为研究区,选择2000、2010、2020年3期土地利用数据,从土地利用动态度和转移矩阵两方面分析土地利用时空变化,运用CLUE-S模型对研究区2030年土地利用变化格局进行模拟验证,设基线、农业发展和生态保护3种情景预测2030年土地... 以挠力河流域为研究区,选择2000、2010、2020年3期土地利用数据,从土地利用动态度和转移矩阵两方面分析土地利用时空变化,运用CLUE-S模型对研究区2030年土地利用变化格局进行模拟验证,设基线、农业发展和生态保护3种情景预测2030年土地利用变化格局,最后对3种情景下耕地适宜性进行评价。结果表明:研究区土地利用类型以耕地为主,2000—2020年森林、湿地、水体和人造地表呈增加趋势,耕地和草地呈减少趋势。2000—2020年土地利用转移主要发生在耕地、草地和森林之间,2000—2010年草地面积转入最多,耕地次之,森林最少,转出面积相反;2010—2020年森林转入转出面积最多,草地次之,耕地最少。CLUE-S模型对挠力河流域土地利用变化具有良好的模拟能力,Kappa系数为0.894,模拟总体精度为91.18%;基线情景下耕地、草地和水体地类面积减少,其他地类面积增加;农业发展情景下耕地面积增加23.68%,人造地表面积未发生变化,其他地类面积均较少;生态保护情景下与农业发展情景正好相反。依评价指标体系和评价模型,分析计算出3种情景下耕地位于适宜区的面积占研究区总面积的96%以上,不适宜区占比低于4%。研究结果可为加强未来耕地质量建设与管理、落实耕地保护目标和科学管理土地资源提供科学决策。 展开更多
关键词 耕地适宜性评价 CLUE-s模型 转移矩阵 土地利用变化 挠力河流域
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The Research of the Relationship Between Yangming’s Yixue and His Xinxue
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作者 LIAO Yiming 《Cultural and Religious Studies》 2023年第4期174-182,共9页
Yangming’s Yixue had been developed several times in his life since originated in Longchang.Yangming’s Yixue and his Xinxue are both sides of the same corn.The Yangming’s Yixue can be roughly divided into two perio... Yangming’s Yixue had been developed several times in his life since originated in Longchang.Yangming’s Yixue and his Xinxue are both sides of the same corn.The Yangming’s Yixue can be roughly divided into two periods.There were the early and middle period of Yangming’s Yixue,which is intended to explain the principles of Yi Zhuan(《易传》)with the theory of Xinxue.The finish of Yangming’s Yixue was a second stage in Yangming’s later years.From the perspective of form and content,there are differences that cannot be ignored between Yangming’s early and middle period and his later years.In the first stage of Yangming’s study,the“Virtuousness”(贞)in Book of Changes is the principle and nature in university.In the later period,Yangming had not focused on interpreting the“Yi Zhuan”,but on the interpretation of ZhouYi’s(《周易》)principles to supplement the connotation of the propositions of Xinxue.This article intends to understand the relationship between Yangming’s Yixue and his Xinxue by dividing the different stages of Yangming’s Yixue firstly.Then by focusing on the influence of Yangming’s later years’Yixue on his Xinxue,we can observe the relation between his Yixue and Xinxue. 展开更多
关键词 Yangming’s Yixue Conscience is Yi nothing kind outside the mind the four-sentence teaching
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智能化测绘背景下测绘工程专业本硕博一体化人才培养模式的探索与实践
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作者 王守刚 张莹 +1 位作者 韩福顺 陈国良 《测绘通报》 CSCD 北大核心 2024年第8期172-176,共5页
智能化测绘背景下的测绘工程专业承担着对接国家战略需要、加速学科转型升级、培养行业拔尖创新人才的重要任务。面对当前测绘类人才培养内容重复、周期较长的问题,本文以中国矿业大学测绘工程专业本硕博一体化培养模式为例,从构建一体... 智能化测绘背景下的测绘工程专业承担着对接国家战略需要、加速学科转型升级、培养行业拔尖创新人才的重要任务。面对当前测绘类人才培养内容重复、周期较长的问题,本文以中国矿业大学测绘工程专业本硕博一体化培养模式为例,从构建一体化的课程体系、一体化的培养机制、一体化的实践创新平台和一体化的学生支持体系4个方面,深入探讨了如何培养具有国际视野、公益精神、创新意识、实践能力的智能化测绘拔尖创新人才。 展开更多
关键词 智能化测绘 测绘工程 本硕博一体化 人才培养 模式探索
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SAM产生菌酿酒酵母HYS98发酵动力学及比生长速率控制策略 被引量:12
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作者 张昀羿 李元广 +2 位作者 金建 杨东 沈国敏 《过程工程学报》 EI CAS CSCD 北大核心 2005年第3期322-326,共5页
通过恒化培养对S-腺苷甲硫氨酸(SAM)产生菌酿酒酵母HYS98发酵动力学进行了研究.结果表明,该菌株的生长与限制性基质蔗糖浓度之间符合Monod关系式,最大比生长速率μmax为0.28h-1,饱和常数KS为1.27g/L;当0.005h?1≤μ≤0.11h-1,产物生成... 通过恒化培养对S-腺苷甲硫氨酸(SAM)产生菌酿酒酵母HYS98发酵动力学进行了研究.结果表明,该菌株的生长与限制性基质蔗糖浓度之间符合Monod关系式,最大比生长速率μmax为0.28h-1,饱和常数KS为1.27g/L;当0.005h?1≤μ≤0.11h-1,产物生成模型为qSAM=-1.39μ2+0.19μ,底物消耗动力学模型为qS=μ/0.62+0.012.基于建立的动力学模型,提出了分阶段控制菌体比生长速率的流加培养策略,据此策略在5L反应器中发酵24h,SAM浓度达3.56g/L,而SAM产率达0.15g/(L?h),比文献报道的最高水平高67%. 展开更多
关键词 s-腺苷甲硫氨酸 恒化培养 动力学模型 流加培养
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嗜蓝孢孔菌新种Fomitiporia yanbeiensis S. Guo&L. Zhou液体发酵培养条件研究 被引量:4
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作者 刘晓钢 周林 +4 位作者 徐莉娜 李艳婷 周伟 郭尚 侯雷平 《山西农业大学学报(自然科学版)》 CAS 北大核心 2018年第5期71-76,共6页
[目的]本研究是对Fomitiporia yanbeiensis S.Guo&L.Zhou液体发酵培养基的营养源和培养条件进行探索。[方法]以发酵培养液中的菌球生物量、菌丝生长速度和菌球萌发活力指数为主要指标,通过单因素试验和正交试验对发酵工艺的培养的... [目的]本研究是对Fomitiporia yanbeiensis S.Guo&L.Zhou液体发酵培养基的营养源和培养条件进行探索。[方法]以发酵培养液中的菌球生物量、菌丝生长速度和菌球萌发活力指数为主要指标,通过单因素试验和正交试验对发酵工艺的培养的温度、pH值、碳源、氮源、接种量进行优化筛选,研究最佳的发酵培养基配方,并对发酵菌丝体和子实体的功能性成分测定对比。[结果]液体发酵培养中最佳的发酵培养条件为:温度27℃、起始pH为8.0、接种量为11%、碳源是海藻糖、氮源是豆粕粉,最佳的培养基配方为海藻糖2.0%、豆粕粉3.5%、KH_2PO_4(0.05%)、MgSO_4(0.025%),该条件下菌球生物量最高为2.36g·100mL^(-1)。发酵菌丝体的三萜类化合物、β-葡聚糖和黄酮含量均高于子实体。[结论]通过对发酵工艺的筛选优化,为该菌的人工驯化和功能性食品开发奠定了基础。 展开更多
关键词 Fomitiporia yanbeiensis s. Guo&L. ZHOU 液体发酵 培养条件
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酒精酵母Saccharomyces cerevisiae 4-S的高密度培养及其酒精发酵性能 被引量:9
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作者 段学辉 牛春铃 欧阳军梅 《南昌大学学报(工科版)》 CAS 2008年第4期311-315,共5页
实验比较了分批培养、分批补料及连续流加培养对酒精酵母细胞生长的影响。结果显示,分批补料流加或连续流加(流加速率0.133 g.L-1.m-1)有利于酒精酵母菌Saccharomyces cerevisiae4-S的快速生长和得到较高的细胞浓度,分批补料流加或连续... 实验比较了分批培养、分批补料及连续流加培养对酒精酵母细胞生长的影响。结果显示,分批补料流加或连续流加(流加速率0.133 g.L-1.m-1)有利于酒精酵母菌Saccharomyces cerevisiae4-S的快速生长和得到较高的细胞浓度,分批补料流加或连续流加培养44 h,发酵液中酒精酵母细胞浓度分别达到71.82(细胞干重-DCW)g.L-1和82.01(DCW)g.L-1,比分批培养分别提高了155.68%和191.95%。同时,对高密度酒精酵母的酒精发酵性能和循环利用研究结果显示,在细胞浓度为82.01(DCW)g.L-1,糖浓度为350 g.L-1,发酵36h发酵液中乙醇浓度达到16.23%,比普通酵母浓度28.09(DCW)g.L-1批发酵时间缩短12 h,乙醇浓度提高了24.4%,酵母的有效重复利用次数达4批次。 展开更多
关键词 酒精酵母 高密度培养 发酵性能
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环磷酰胺激活小鼠肝微粒体谷胱甘肽S-转移酶Ⅰ的机制研究 被引量:2
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作者 郑英 邹晓华 +3 位作者 付再林 方慧 陈素红 吕圭源 《中国药理学通报》 CAS CSCD 北大核心 2013年第10期1387-1390,共4页
目的探讨环磷酰胺(CP)在体内对小鼠肝微粒体谷胱甘肽S-转移酶Ⅰ(mGST-Ⅰ)活性的影响及其可能的机制。方法用苯巴比妥75 mg·kg-1诱导小鼠CYP2B后,ip CP,测定9 h内mGST-Ⅰ酶活性,观察二巯基丁二醇(DTT)对酶激活的逆转作用和巯基烷化... 目的探讨环磷酰胺(CP)在体内对小鼠肝微粒体谷胱甘肽S-转移酶Ⅰ(mGST-Ⅰ)活性的影响及其可能的机制。方法用苯巴比妥75 mg·kg-1诱导小鼠CYP2B后,ip CP,测定9 h内mGST-Ⅰ酶活性,观察二巯基丁二醇(DTT)对酶激活的逆转作用和巯基烷化剂N-乙基马来酰亚胺(NEM)对酶再激活效应,用SDS-PAGE和负染凝胶法评价CP对mGST-Ⅰ蛋白表达的影响。结果 CP引起小鼠微粒体中mGST-Ⅰ活性增加;NEM在mGST-Ⅰ半胱氨酸-49-巯基(cys-49-SH)上的活化效应减弱,而CP引起的mGST-Ⅰ激活效应不被二硫键还原剂DTT逆转。激活的mGST-Ⅰ电泳图谱未见蛋白分子量变迁及蛋白表达增加。结论大剂量CP致mGST-Ⅰ激活效应机制主要是酶分子cys-49-SH上单个巯基的修饰作用。 展开更多
关键词 环磷酰胺 微粒体 谷胱甘肽s-转移酶Ⅰ 二巯基丁二醇 N-乙基马来酰亚胺 半胱氨酸-49-巯基 机制
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Chemostat系统中的Hopf分支 被引量:7
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作者 刘婧 郑斯宁 《大连理工大学学报》 CAS CSCD 北大核心 2002年第4期387-390,共4页
讨论了一类微生物连续培养模型的 Hopf分支问题 .该模型的消耗率参数中含有一个关于营养基的二次函数 ,功能反应函数则为具有内代谢的 Monod类型 ,以更好地模拟实际问题 .利用 Friedrich方法得到了该系统存在 Hopf分支的条件 ,并判定了... 讨论了一类微生物连续培养模型的 Hopf分支问题 .该模型的消耗率参数中含有一个关于营养基的二次函数 ,功能反应函数则为具有内代谢的 Monod类型 ,以更好地模拟实际问题 .利用 Friedrich方法得到了该系统存在 Hopf分支的条件 ,并判定了周期解的稳定性 . 展开更多
关键词 Chemostat系统 周期解 稳定性 Friedrich方法 微生物连续培养模型 HOPF分支 Monod模型
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