Research on the Adaptability of New Flue-cured Tobacco Varieties(Lines) in Ankang Tobacco Growing Area

In order to screen new flue-cured tobacco varieties （lines） with mountain characteristics of Ankang tobacco growing area, the adaptable selection experiments of 6 newly introduced flue-cured tobacco varieties （lines） were carried out at the 2 set test sites in Ankang producing areas with K326, ＇Yunyan 87＇ and ＇Qinyan 96＇ as controls. The results showed that YN105 had more leaves per plant and coordi- nate chemical composition, and the yield per unit area was 2 221.05 kg/hm2 with the single output of 33 960.00 yuan/hm^2. Its rate of high and medium class leaves was 80.17%, presenting good performances in testing varieties （lines）. The flowering of YN99 was late but showed coordinate chemical composition, and the yield per unit area was 2 128.50 kg/hm^2 with the single output of 33 454.05 yuan/hm^2. Its rate of high and medium class leaves was 83.17%, which also had good perfor- mances in testing varieties （lines）. It was recommended that YN105 and YN99 be moderate planted in Ankang tobacco growing area, but further experiment is needed to confirm.

Screening of Tobacco Varieties/Lines Suitable for Ankang Tobacco-growing Area of Shaanxi Province

In order to screen excellent tobacco varieties/lines that can be promoted in Ankang tobacco-growing area, this article analyzed the growth stages, botanical characteristics, agronomic characteristics, disease resistance, flue-cured leaf appearance and economic characteristics of five tobacco varieties （lines）： Yunyan 99, Yunyan 105, 6517, PVH19 and K326. The results showed that, compared with the control K326, Yunyan 105 had good appearance of flue-cured leaves, long growth duration and high disease resistance; its floral buds appeared early; it produced 22.8 leaves per plant, high yield of 2 134.5 kg/hm2 and high economic output of 39 274.8 Yuan/hm2. The percentage of first-class leaves of Yunyan 105 reached 39.07%, and the weight per leaf was 8.4 g. Yunyan 99 also had good appearance of flue-cured leaves, large stem diameter, large middle leaves high disease resistance; the sand leaves of Yunyan 99 matured early; its tobacco leaf yield was 2 058 kg/hm2, and its economic output reached 36 838.5 Yuan/hm2; the percentage of first-class leaves of Yunyan 99 was 40.4%. In summary, both Yunyan 105 and Yunyan 99 performed well in all tested traits, and thus can be selectively planted in Ankang tobaccogrowing area.

Principal Component Analysis and Cluster Analysis of Fagopyrum tataricum Varieties(Lines)

In order to reveal the genetic differences and agronomic traits of Fagopy-rum tataricum_ varieties （lines） intuitively, explore good resources and avoid the blindness of parent selection during the breeding process, six primary agronomic traits of 45 F. tataricum_ varieties （lines） that came from the eleven buckwheat breeding departments across the country were analyzed with principal component analysis and cluster analysis. The results of principal component analysis showed that the six agronomic traits could be simplified into three principal components, and the cumulative contribution rate reached 83%. The results of cluster analysis showed that the 45 F. tataricum varieties （lines） were classified into four groups：high stalk, medium yield and smal grain type, medium stalk, high yield and large grain type, medium stalk, low yield and smal grain type and high stalk, medium yield and medium grain type. Among them, performance of comprehensive trait of the second type was better than that of the other types. Thus, the F. tataricum_va-rieties （lines） that were classified into the second type could be considered as good varieties （lines） or breeding materials. The genetic differences among F. tataricum_varieties （lines） had no necessary correlations with origin and geographical distance. ln addition to complementary traits and geographical distance, genetic distances （dif-ferent populations） should be taken into consideration during parent selection in cross breeding.

Characterization of QTL for unique agronomic traits of new-plant-type rice varieties using introgression lines of IR64

To enhance the yield potential of an elite indica rice cultivar,an introgression(BC_3-derived) line of IR64,YTH288,was developed using a new-plant-type cultivar,IR66215-44-2-3,as a donor parent.YTH288 has agronomically valuable characteristics such as large panicles,few unproductive tillers,and large leaves inherited from NPT.To identify the genetic basis of these traits,we used 167 F_2 plants derived from a cross between IR64 and YTH288 to conduct QTL analysis for five agronomic traits:days to heading(DTH),culm length(CL),flag leaf length(FLL),flag leaf width(FLW),and filled spikelet number per panicle(FSN).Six putative QTL were detected:four on chromosome4(for CL,FLL,FLW,and FSN) and two on chromosome 2(for DTH and FLL).All QTL with the IR66215-44-2-3 allele,except that for FLL on chromosome 2,had positive effects on each trait.To confirm the effects of these putative QTL,we developed NILs with the IR64 genetic background by marker-assisted selection.We observed significant differences in several agronomic traits between IR64 and NILs that carried these QTL on chromosomes2 and 4.Additionally,four IR64-NILs carrying chromosomal segments derived from different NPT varieties on the long arm of chromosome 4 exhibited similar pleiotropic To enhance the yield potential of an elite indica rice cultivar, an introgression(BC3-derived) line of IR64, YTH288, was developed using a new-plant-type cultivar,IR66215-44-2-3, as a donor parent. YTH288 has agronomically valuable characteristics such as large panicles, few unproductive tillers, and large leaves inherited from NPT.To identify the genetic basis of these traits, we used 167 F2 plants derived from a cross between IR64 and YTH288 to conduct QTL analysis for five agronomic traits: days to heading(DTH), culm length(CL), flag leaf length(FLL), flag leaf width(FLW), and filled spikelet number per panicle(FSN). Six putative QTL were detected: four on chromosome4(for CL, FLL, FLW, and FSN) and two on chromosome 2(for DTH and FLL). All QTL with the IR66215-44-2-3 allele, except that for FLL on chromosome 2, had positive effects on each trait. To confirm the effects of these putative QTL, we developed NILs with the IR64 genetic background by marker-assisted selection. We observed significant differences in several agronomic traits between IR64 and NILs that carried these QTL on chromosomes2 and 4. Additionally, four IR64-NILs carrying chromosomal segments derived from different NPT varieties on the long arm of chromosome 4 exhibited similar pleiotropiceffects for unique agronomic traits. These NILs can be used as research materials for studying each trait and as breeding materials for yield improvement of indica rice cultivars.

Nutritional and Technological Characteristics of New Cowpea (<i>Vigna unguiculata</i>) Lines and Varieties Grown in Eastern Kenya

Protein sources in the diet of people living in semi-arid land of Kenya are lacking and if available it is costly to them. In terms of safe food and a healthy food supply, cowpeas (Vigna unguiculata.) are a significant source of protein, carbohydrates, and minerals especially for poor populations in the Kenya, it is said to be poor man’s meat. The aim of this study was to determine nutritional composition of newly bred ten cowpea lines and five varieties commonly grown in Eastern Kenya of Kitui, Machakos and Makueni counties to understand their potential utilization in curbing rising food and nutrition insecurity in arid and semi-arid lands ASALs and in any other food applications in Kenya. The experiment was arranged in Completely Randomized Design (CRD) whereby proximate composition and minerals were determined using standard AOAC and AACC methods and technological characteristics checked using modified methods used by other researchers. Collected data were subjected to Analysis of Variance (ANOVA) using SAS (2006) version 9.1, mean separation was done using Tukey’s Honestly Significant Difference (HSD) method at 5% level of significance. Cowpeas composition ranged from 12.28% - 13.35% for moisture content, 49.37% - 55.74% for total carbohydrates, 2.99% - 3.34% for crude ash, 0.13% - 0.81% for crude lipids, 23.37% - 29.70% for crude protein and 1.40% - 4.34% for crude fibers. Cowpea samples recorded highest percentage of essential amino acids (60.71%) and non-essential amino acids (39.29%). Minerals ranged from 1.97 - 2.69 mg/100g for calcium, 3.23 - 3.90 mg/100g for magnesium, 205.53 - 223.30 mg/100g for sodium, 0.80 - 1.23 mg/100g for zinc, 1071.15 - 1152.62 mg/100g for potassium and 0.62 - 1.06 mg/100g for phosphorus. For technological properties, lines absorbed water equivalent to their weights and they were comparable to varieties grown in the region. From the results it showed that cowpea line IT97K-1042-3, TEXAN PINKIYE, TX123, IT85F-867-5, IT82D-889-1 and IT82D-889 have desirable attributes such as high crude protein contents, good water absorption capacities and volumetric expansion. They compared well with existing K80 variety. These cowpea lines could possibly be bred and combined into a single cowpea line and further improved by breeders to have other good properties such as higher levels of water absorption during soaking hence reduced cooking times. Therefore, this work has shown that cowpea lines developed can be used as food security crop, industrial food applications and enriching foods of low protein like in complementary foods for healthy food supply in Eastern Kenya.

Correlation of Inhibitor Proteinase in Varieties and Lines of Cotton(Gossypium hirsutum L.) to Different Geographic Population of Verticillium dathliae Klebahn

Breeding for wilt resistance and its theoretical basis are primarily responsible for increases in cotton yield and fiber quality. Breeding for immunity is the most efficient method in our struggle with infectious diseases.

NEW VARIETIES AND LINES

Ⅱ-you 92: a new hybrid rice for late seasonⅡ-you 92 is an indica hybrid rice that was developed by Jinhua Agri Res Inst, Zhejiang Province.Ⅱ-you 92 has a suitable growth duration of 122-125 d as double-cropping late season rice, 3-4 dshorter than that of Shanyou 64 and 6-7 d shorter than Shanyou 10. It has a compact plant type, high tiller-ing ability and plant height of 85-90 cn. A number of 3.4-3.6 million effective panicles per ha can be

NEW VARIETIES AND LINES

Qixiuzhan 3:a new high-yielding, good-quality indica rice varietyBy crossing Qishanzhan with Xinxiu 299, a new variety Qixiuzhan 3 was developed by the Rice Res Inst ofGuangdong Acad of Agri Sci.Being planted in the early season, this variety belongs to mid-late-maturity rice, with the same ma-

Cultivar Mixture Cooperation(CMC)of rice varieties(lines) with different blast resistant gene

We studied Cultivar Mixture Cooperation(CMC)of rice varieties(lines)by screening mixture ofrice varieties which possess different genes,1983-1996.

Evaluation and screening for resistance of some varieties(lines)to major diseases and insect pests of rice

During 1984-1988,2,231 varieties(lines)from International Rice Testing Program(IRTP)were evaluated and screened for resistance to riceblast(Bl),bacterial blight(BB),sheath blight

Variance Analysis of Growth Characteristics and Partial Production and Quality Characters of Different Tobacco Varieties

Objective] This study was conducted to discuss growth characteristics and partial production and quality characters of different varieties, so as to provide can-didates for tobacco production as wel as a theoretical basis for variety deployment in Guizhou. [Method] ‘K326’ was used as control, the experiment for variance analysis of growth characteristics and partial production and quality characters of dif-ferent tobacco varieties was carried out in Fengsan Town, Kaiyang County, Guizhou Province in 2015. [Result] GY8 grew faster in early stages and slower in late stages, the growth periods of GY2 （130 d） and GY5（129 d） were the longest, while those of Yun85 （121 d） and Yun87 （121 d） were the shortest; and GY2, GY5 and GY8 had larger plant heights, maximum leaf widths and maximum leaf areas, and Yun85, Yun87 and K326 showed larger stem girths and maximum leaf lengths. Yun87 exhibited lower natural incidences of diseases, while Yun85 and GY8 showed higher incidences of diseases. GY2, GY5 and GY8 had higher nicotine, total nitro-gen, K and Cl contents, while Yun85, Yun87 and K326 had higher total sugar and reducing sugar contents and higher total sugar/nicotine ratio. Yun85 and Yun87 showed better economic characters, while GY5 and GY8 showed poorer economic characters. [Conclusion] Yun85 and Yun87 have quality characters better than GY2 and GY8, field natural incidences of diseases lower than GY2 and GY8, and better economic characters, and thus could serve as candidates in future flue-cured tobac-co production in Guizhou.

Screening of Low Tar Flue Cured Tobacco Varieties

The main agronomic traits,economic traits,appearance quality,main chemical components,gas composition and physical parameters of 10 flue cured tobacco varieties were investigated in the experiment.And the field natural incidence of the main diseases was investigated.The results showed that main agronomic characters of the 10 varieties were in line with the breeding objectives,among which 5 varieties have good economic characters;5 varieties have good appearance quality;6 varieties have coordinated chemical composition and high potassium content;9 varieties have good smoke components and physical indicators.

Adoption and Impact of Tobacco Recommended Varieties among Smallholder Farmers in Tabora Region

The research examines the status of adoption of improved tobacco recommended varieties among smallholder farmers with a view to assess the impact of tobacco production on household income in the region. A survey approach was used to collect data from a sample of 160 tobacco farmers who were randomly selected. Descriptive statistics and logistic regression model were used to analyze the data. Results show that farmers have responded appreciably to intervention programme that promote the use of the tobacco recommended varieties with an adoption rate of 61.6%. Information flow from various tobacco companies was the significant factor influencing the decision of farmers to adopt the tobacco recommended varieties. Constraints in the adoption noted include poor germination of seeds, high demand of inputs, unreliable availability of seeds and inadequate knowledge on tobacco production. The mean household income from tobacco production （4,232,415 Tshs） was significantly higher than the mean income of other income sources cumulatively （211,333 Tshs） with an income advantage of 4,021,082 Tshs. The study concludes that farmers are responding appreciably to intervention programmes that promote the use of tobacco recommended varieties in the region. Also tobacco sub-sector has higher contribution on household total income as compared to other enterprises.

Excavation and Screening Utilization of Strong Aroma Tobacco Variety in Chizhou Tobaccogrowing Area

[Objective] This study was aimed to screen out the strong-aroma tobacco variety with good ecological adaptability as the best cultivar in Chizhou. [Method] Based on the ecological conditions that formed the strong aroma style of tobacco leaves in the tobacco-growing area, 6 varieties（K326, NC55, NC71, 0508, Yunyan99, Yan240） were selected and compared through combining the demand characteristics of industrial enterprise to the quality. [Results] The output value of 0508 was the highest and NC71 had a medium output value but with coordinated chemical composition. The system evaluation of this study on the quality and characteristic style further clarified the feature strength and utility value of various tobacco varieties with strong aroma. [Conclusion] NC71 is the best cultivar in Chizhou tobacco-growing area, and it is suggested using NC71 as the demonstrative variety of the next year. The study on the excavation and screening utilization of tobacco varieties with local features is of great significance to the tobacco production industry.

Identification of Quality Characteristics of Sun-cured Tobacco Leaves

[Objective] The aim was to explore tobacco varieties which satisfy tobacco formula and is suitable to be grown in ecotopes simultaneously. [Method] Interior and exterior qualities of tobacco were compared and identified based on agronomic and economic characters of tobacco in fields. [Result] Yunshai No.1 was better in both agronomic and economic characters. Specifically, the fragrance was dominated by empyreumatique and aroma; the highly-qualified variety is fragrant and sweet, with little irritation, which gives the tobacco smoke a light, mild flavor. As for Virgini- a934, economic characters were better, but agronomic characters were just ordinary. Similar to Yunshai No.l, dominated by empyreumatique and aroma, the variety was with distinctive resin and herbaceous fragrance. What＇s more, it tasted good with high quality and usability. Cunsanpi was ordinary in agronomic characters, and poor in economic characters, with coordinated quality and chemical composition. It is fra- grant and the smoke is clean. However, the quality was not so high and it tasted not so good. Tengruan No.2 was good in agronomic characters, but poor in eco- nomic characters, which gives delicate fragrance, but not clean enough. Waiweiba and Mijie Qinggeng neither taste well nor smell sweet, performing ordinary in agro- nomic and economic characters. Hence, the two varieties seem bad in quality and ranks poorly. [Conclusion] In terms of sensory quality, the varieties from high to low were Yunshai No.1, Virginia 934, Cunsanpi, Tengruan No.2, Waiweiba and Mijie Qinggeng.

The technics of breeding a new transgenic tobacco cultivar resistant to mosaic diseases and its extention

This paper reports a new transgenic tobacco line NC89 resistant to both tobaccomosaic virus(TMV)and cucumber mosaic virus(CMV).It deals with the details of field experi-ments of the whole process of segregation and selection of the progenies of transgenic plant popu-lation.By using the gene transfer technics,some transgenic plants resistant to mosaic disease froma flue-cured tobacco cultivar NC89 were obtained in green house in 1989.Thereafter,throughfield inoculation and selection from the 2nd and 3rd generations,a homozygous line resistant toboth viruses and with good qualities was bred out.The inheretant stability of the resistance of thenew line was confirmed through the 4th to 6th generations.In 1992,the transgenic resistant linewas planted about 7000 hectares in Henan province.The relative effect of controlling mosaic dis-eases of tobacco was 50—70%.The out-put value increased 30—50% per unit area.This is a newexample of growing a transgenic virus-resistant Plant in large scale in China.

Defoliation in Flue-cured Tobacco and Its Reasons

The flue-cured tobacco G80 is not only one of the characteristic varieties in Changsha, but also indispensable raw materials for the series of Baisha ciga- rette. Defoliation during the late stage of growth seriously reduces the yield and quality of tobacco, and limits the popularization and application of G80. In this pa- per, in order to investigate the reasons for the defoliation of G80, the transverse section structure, cellulose content and differential proteins of leaf major veins were analyzed. The results revealed that the autolysis and collapse of parenchyma cells is the main cause of defoliation, and fimgal infection is a factor inducing the au- tolysis of parenchyma cells. Application of bud suppression agent is one of the main ways to prevent fungal infection. The low cellulose content, low synthetic ability of xylan and pectin, the low removal ability of abnormal protein and low immune responsiveness are the inner reason for defoliation of flue-cured tobacco G80.

Breeding of Maintainer Line and Sterile Line Brown Planthopper Resistant Rice Using Marker-assisted Selection

Guangxi common wild rice variety brown planthopper highly resistant introgression line HS204 was taken as antigen donor material,hybridization,backcrossing,and molecular marker-assisted selection methods were adopted,to select maintainer line and sterile line materials,so as to provide excellent material foundation for resistance breeding of hybrid rice. Through the marker-assisted selection,it obtained 4 pieces of homozygous resistance gene maintainer line intermediate materials( 100B,101B,102B and 103B),and 2 pieces of resistant sterile line materials( 100A and 103A). All 10 combinations that have testcross with highly resistant sterile 100A showed higher level of brown planthopper resistance: 100A/R2586,100A/KR838,and 100A/KR527 had high resistance level,the others had low to intermediate resistance;100A/KR527,100A/R2586,100A/Minghui 63,100A/Fuhui 838 and 100A/Gui 99 combinations had yield per plant significantly higher than the control group( Teyou 7118),increasing by 14. 45%-49. 26%. The obtained resistant lines are expected to provide a better gene source for the breeding of resistant sterile lines of hybrid rice and the obtained resistant sterile lines can be directly used in the selection of three-line hybrid rice.

Study on the Ecological Adaptability of Fluecured Tobacco Variety Longjiang237 in Heilongjiang Tobacco Area

The research explored indices of Longjiang237, including agronomic characters, characters in growth period, tolerance to diseases, economic characters, appearance quality, physical characters, chemical components, and sensory quality,with NC89 as a control. The results showed that Longjiang No.237, immune to TMV, grew strongly, with a long growth term, and had low demands on nitrogen.Besides, Longjiang237 takes advantages in terms of yield, grade index and output value index, which grew by 10.46%, 8.00% and 18.41%, respectively. Additionally,highlights should be given to potassium, aroma quality, odor, strength, irritation, and aftertaste of tobaccos.

烤烟新品种川烟101的选育及特征特性

烤烟新品种川烟101(原编号A601)是以(红花大金元×RG11)F_(1)为母本、(云烟85×K346)F_(1)为父本杂交,以提高品种黑胫病抗性、烟叶易烤性为主要目标,经系谱法选育而成的烤烟纯系新品种。该品种田间长势强,植株塔形,叶片开片较好,适应性广,成熟特性好,易烘烤,中抗黑胫病和赤星病。综合经济性状显著优于主对照品种K326和副对照品种云烟87;综合感官质量、物理特性优于主对照品种K326和副对照品种云烟87,香型风格凸显程度较强;综合质量与主对照K326相当,优于副对照云烟87。川烟101是一个抗性、产量和品质都较好的优良烤烟纯系新品种,适合在西南烟区、东南烟区推广种植。