In this study, we reported the repaid construction of a molecular marker linkage map of rice (Oryza sativa L.). An F-2 population from the cross between Annong S-1 and Nanjing 11 was used to construct a genetic linkag...In this study, we reported the repaid construction of a molecular marker linkage map of rice (Oryza sativa L.). An F-2 population from the cross between Annong S-1 and Nanjing 11 was used to construct a genetic linkage map of rice. Total of 142 newly screened AFLP markers and 30 anchor markers (25 SSR markers and 5 RFLP markers) were mapped on the 12 chromosomes covering 1537.4 cM of rice genome. The average interval between these markers was 9.0 cM. The total work which usually was finished in more than one year was finished within only 3 months by one person. This is the first plant AFLP map developed in China. A new thermosensitive genic male sterile gene in rice, tms5, was Egged and mapped onto chromosome 2 during the development of the linkage map.展开更多
Previous study indicated that the thermo-sensitive genic malesterile(TGMS) gene in rice was regulated by temperature.TGMS rice plays an important role in hybrid rice production,because the application of the TGMS syst...Previous study indicated that the thermo-sensitive genic malesterile(TGMS) gene in rice was regulated by temperature.TGMS rice plays an important role in hybrid rice production,because the application of the TGMS system in two-line breeding is laborsaving,timesaving,simple,inexpensive,efficient,and eliminating the limitations of the cytoplasmic male sterility(CMS) system.'AnnongS' is the first discovered and deeply studied TGMS rice lines in China.'AnnongS-1' and 'Y58S',two derivatives of TGMS line AnnongS,were both controlled by a single recessive gene named tms5,which was genetically mapped on chromosome 2.In this study,three populations('AnnongS-1' × 'Nanjing11','Y58S' × 'Q611',and 'Y58S' × 'Guanghui122') were developed and used for the molecular fine mapping of the tms5 gene.By analyzing recombination events in the sterile individuals using a total of 125 probes covering the tms5 region,the tms5 gene was physically mapped to a 19-kb DNA fragment between two markers 4039-1 and 4039-2,which were located on the BAC clone AP004039.After the construction of the physical map between two markers 4039-1 and 4039-2,a member(ONAC023) of the NAC(NAM-ATAF-CUC-related) gene family was identified as the candidate gene of the tms5 gene.展开更多
Based on topic maps, a preprocessing scheme using similarity comparision is presented and applied in knowledge management. Topic and occurrence-ofiented merging algorithm is also introduced to implement knowledge inte...Based on topic maps, a preprocessing scheme using similarity comparision is presented and applied in knowledge management. Topic and occurrence-ofiented merging algorithm is also introduced to implement knowledge integration for the sub-system. AnOm- nigator-supported example from an aeroaustic institute is utilised to validate the preprocessing method and the result indicates it can speed up the research schedule.展开更多
Characterized by lithological diversity and rich mineral resources, Benshangul-Gumuz National Regional State located in Asosa Zones, Western Ethiopia has been investigated for geological mapping and morpho-structural ...Characterized by lithological diversity and rich mineral resources, Benshangul-Gumuz National Regional State located in Asosa Zones, Western Ethiopia has been investigated for geological mapping and morpho-structural lineaments extraction using PALSAR (Phased Array type L-band Synthetic Aperture Radar ) Fine Beam Single (FBS) L-HH polarization and Landsat-5 TM (Thematic Mapper ) datasets. These data were preprocessed to retrieve ground surface reflectance and backscatter coefficients. To overcome the geometry acquisition between the two sensors, they were geometrically and topographically rectified using ASTER-V2 DEM. Intensity-Hue-Saturation, directional filters and automatic lineaments extraction were applied on the datasets for lithological units’ discrimination and structural delimitation for potential mineral exploration. The obtained results showed good relationship among the topographic morphology, rock-substrate, structural variations properties, and drainage network. The spectral variations were easily associated with lithological units. Likewise, the morpho-structural information highlighted in the PALSAR image was visible without altering the radiometric integrity of the details in TM bands through the fusion process. Moreover, predominant lineaments directions trending NE-SW, NS, and NW-SE were identified. Results of this study highlighted the importance of the PALSAR FBS L-HH mode and TM data fusion to enhance geological features and lithological units for mineral exploration particularly in tropical zones.展开更多
The Indian shield comprises a number of Archean–Paleoproterozoic cratonic blocks and predominantly Meso–Neoproterozoic mobile belts with Archean protoliths.All these ancient cratons were thought to be integral parts of
文摘In this study, we reported the repaid construction of a molecular marker linkage map of rice (Oryza sativa L.). An F-2 population from the cross between Annong S-1 and Nanjing 11 was used to construct a genetic linkage map of rice. Total of 142 newly screened AFLP markers and 30 anchor markers (25 SSR markers and 5 RFLP markers) were mapped on the 12 chromosomes covering 1537.4 cM of rice genome. The average interval between these markers was 9.0 cM. The total work which usually was finished in more than one year was finished within only 3 months by one person. This is the first plant AFLP map developed in China. A new thermosensitive genic male sterile gene in rice, tms5, was Egged and mapped onto chromosome 2 during the development of the linkage map.
文摘Previous study indicated that the thermo-sensitive genic malesterile(TGMS) gene in rice was regulated by temperature.TGMS rice plays an important role in hybrid rice production,because the application of the TGMS system in two-line breeding is laborsaving,timesaving,simple,inexpensive,efficient,and eliminating the limitations of the cytoplasmic male sterility(CMS) system.'AnnongS' is the first discovered and deeply studied TGMS rice lines in China.'AnnongS-1' and 'Y58S',two derivatives of TGMS line AnnongS,were both controlled by a single recessive gene named tms5,which was genetically mapped on chromosome 2.In this study,three populations('AnnongS-1' × 'Nanjing11','Y58S' × 'Q611',and 'Y58S' × 'Guanghui122') were developed and used for the molecular fine mapping of the tms5 gene.By analyzing recombination events in the sterile individuals using a total of 125 probes covering the tms5 region,the tms5 gene was physically mapped to a 19-kb DNA fragment between two markers 4039-1 and 4039-2,which were located on the BAC clone AP004039.After the construction of the physical map between two markers 4039-1 and 4039-2,a member(ONAC023) of the NAC(NAM-ATAF-CUC-related) gene family was identified as the candidate gene of the tms5 gene.
文摘Based on topic maps, a preprocessing scheme using similarity comparision is presented and applied in knowledge management. Topic and occurrence-ofiented merging algorithm is also introduced to implement knowledge integration for the sub-system. AnOm- nigator-supported example from an aeroaustic institute is utilised to validate the preprocessing method and the result indicates it can speed up the research schedule.
文摘Characterized by lithological diversity and rich mineral resources, Benshangul-Gumuz National Regional State located in Asosa Zones, Western Ethiopia has been investigated for geological mapping and morpho-structural lineaments extraction using PALSAR (Phased Array type L-band Synthetic Aperture Radar ) Fine Beam Single (FBS) L-HH polarization and Landsat-5 TM (Thematic Mapper ) datasets. These data were preprocessed to retrieve ground surface reflectance and backscatter coefficients. To overcome the geometry acquisition between the two sensors, they were geometrically and topographically rectified using ASTER-V2 DEM. Intensity-Hue-Saturation, directional filters and automatic lineaments extraction were applied on the datasets for lithological units’ discrimination and structural delimitation for potential mineral exploration. The obtained results showed good relationship among the topographic morphology, rock-substrate, structural variations properties, and drainage network. The spectral variations were easily associated with lithological units. Likewise, the morpho-structural information highlighted in the PALSAR image was visible without altering the radiometric integrity of the details in TM bands through the fusion process. Moreover, predominant lineaments directions trending NE-SW, NS, and NW-SE were identified. Results of this study highlighted the importance of the PALSAR FBS L-HH mode and TM data fusion to enhance geological features and lithological units for mineral exploration particularly in tropical zones.
文摘The Indian shield comprises a number of Archean–Paleoproterozoic cratonic blocks and predominantly Meso–Neoproterozoic mobile belts with Archean protoliths.All these ancient cratons were thought to be integral parts of
