[Objective]The aim was to reveal the spatial distribution characteristics of total nutrients in soil and provide a theoretical basis for farmland management and improvement of crop yield. [Method]GIS technique was use...[Objective]The aim was to reveal the spatial distribution characteristics of total nutrients in soil and provide a theoretical basis for farmland management and improvement of crop yield. [Method]GIS technique was used to analyze the spatial distribution characteristics of total C,total N,total P and total K for different soil layers in Liaoning Province. [Result]The results showed that the content of total C,total N,total P decrease from east to west,but the content of total K was high in north district of Liaoning Province. The content of total C,total N,total P and total K was higher in soil surface (0-20 cm) than the lower (20-40 cm). Total K varied less with soil depth,and its mean content was respectively 17.64 g/kg and 17.08 g/kg for soil surface and soil lower layer. [Conclusion]The results of the distribution of soil total nutrients in different soil layers supplied a theory basis for farmland management.展开更多
Soil sediment samples of 10 layers with a spacing of 10 cm each were collected in different floodplain zones adjacent to Huolin River in the Xianghai Nature Reserve, and contents of total N, total P and organic matter...Soil sediment samples of 10 layers with a spacing of 10 cm each were collected in different floodplain zones adjacent to Huolin River in the Xianghai Nature Reserve, and contents of total N, total P and organic matters were analyzed. The results showed that contents of total N, total P and organic matters were generally decreasing with the increase of distance from sample locations to the river channel, and contents of the three items were generally higher in the upper soil layer than that in the lower soil layer. The content variations displayed how flooding functions influenced nutrient matter content variations in floodplain soils since the flood inundation frequencies of the sample locations varied. The correlation analysis displayed that there were remarkable relativities between total N, total P and organic matters within definite spatial distance from the Huolin River channel.展开更多
Soil organic carbon and nitrogen are used as indexes of soil quality assessment and sustainable land use management. At the same time, soil C/N ratio is a sensitive indicator of soil quality and for assessing the carb...Soil organic carbon and nitrogen are used as indexes of soil quality assessment and sustainable land use management. At the same time, soil C/N ratio is a sensitive indicator of soil quality and for assessing the carbon and nitrogen nutrition balance of soils. We studied the characteristics of soil organic carbon and total nitrogen by investigating a large number of apple orchards in major apple production areas in China. High apple orchard soil organic carbon content was observed in the provinces of Heilongjiang, Xinjiang, and Yunnan, whereas low content was found in the provinces of Shandong, Henan, Hebei, and Shaanxi, with the values ranging between 6.44 and 7.76 g·kg-1. Similar to soil organic carbon, soil total nitrogen content also exhibited obvious differences in the 12 major apple producing provinces. Shandong apple orchard soil had the highest total nitrogen content (1.26 g·kg-1), followed by Beijing (1.23 g·kg-1). No significant difference was noted between these two regions, but their total nitrogen content was significantly higher than the other nine provinces, excluding Yunnan. The soil total nitrogen content for Xinjiang, Heilongjiang, Hebei, Henan, and Gansu was between 0.87 and 1.03 g·kg-1, which was significantly lower than that in Shandong and Beijing, but significantly higher than that in Liaoning, Shanxi, and Shaanxi. Six provinces exhibited apple orchard soil C/N ratio higher than 10, including Heilongjiang (15.42), Xinjiang (13.38), Ningxia (14.45), Liaoning (12.24), Yunnan (11.03), and Gansu (10.63). The soil C/N ratio was below 10 in the remaining six provinces, in which the highest was found in Shaanxi (9.47), followed by Beijing (8.98), Henan (7.99), and Shanxi (7.62), and the lowest was found in Hebei (6.80) and Shandong (6.05). Therefore, the improvement of soil organic carbon should be given more attention to increase the steady growth of soil C/N ratio.展开更多
The southeastern Tibetan Plateau,which profoundly affects East Asia by helping to maintain the stability of climate systems,biological diversity and clean water,is one of the regions most vulnerable to water erosion,w...The southeastern Tibetan Plateau,which profoundly affects East Asia by helping to maintain the stability of climate systems,biological diversity and clean water,is one of the regions most vulnerable to water erosion,wind erosion,tillage erosion,freeze-thaw erosion and overgrazing under global climate changes and intensive human activities.Spatial variations in soil erosion in terraced farmland(TL),sloping farmland(SL)and grassland(GL)were determined by the^(137)Cs tracing method and compared with spatial variations in soil organic carbon(SOC)and total nitrogen(total N).The^(137)Cs concentration in the GL was higher in the 0-0.03 m soil layer than in the other soil layers due to weak migration and diffusion under low precipitation and temperature conditions,while the^(137)Cs concentration in the soil layer of the SL was generally uniform in the 0-0.18 m soil layer due to tillage-induced mixing.Low^(137)Cs inventories appeared at the summit and toe slope positions in the SL due to soil loss by tillage erosion and water erosion,respectively,while the highest^(137)Cs inventories appeared at the middle slope posi-tions due to soil accumulation under relatively flat landform conditions.In the GL,the^(137)Cs data showed that higher soil erosion rates appeared at the summit due to freeze-thaw erosion and steep slope gradients and at the toe slope position due to wind erosion,gully erosion,freeze-thaw erosion and overgrazing.The^(137)Cs inventory generally increased from upper to lower slope positions within each terrace(except the lowest terrace).The^(137)Cs data along the terrace toposequence showed abrupt changes in soil erosion rates between the lower part of the upper terrace and the upper part of the immediate terrace over a short distance and net deposition on the lower and toe terraces.Hence,tillage erosion played an important role in the soil loss at the summit slope positions of each terrace,while water erosion dominantly transported soil from the upper terrace to the lower terrace and resulted in net soil deposition on the flat lower terrace.The SOC inventories showed similar spatial patterns to the^(137)Cs inventories in the SL,TL and GL,and significant correlations were found between the SOC and^(137)Cs inventories in these slope landscapes.The total N inventories showed similar spatial patterns to the inventories of,37Cs and SOC,and significant correlations were also found between the total N and^(137)Cs inventories in the SL,TL and GL Therefore,^(137)Cs can successfully be used for tracing soil,SOC and total N dynamics within slope landscapes in the southeastern Tibetan Plateau.展开更多
Forty soils from top layer (0-20 cm) were sampled in different regions of China and Kjeldahl, HF-Kjeldahl and double treatment methods were used to determine total N, total N plus fixed ammonium, and total N and the r...Forty soils from top layer (0-20 cm) were sampled in different regions of China and Kjeldahl, HF-Kjeldahl and double treatment methods were used to determine total N, total N plus fixed ammonium, and total N and the residual fixed ammonium left in soil after determination of total N, respectively, to evaluate if Kjeldahl’s method could include the fixed N by soil minerals. The fixed N by soil minerals was measured by Silva-Bremner procedure to make comparison. Results showed that total N determined by Kjeldahl’s method averaged 1.622 g kg-1, while that by HF- Kjeldahl’s method 1.633 g kg-1, and that by double procedure 1.666 g kg-1. Obviously results obtained by the last two methods, particularly the double treatment method, were higher than Kjeldahl’s, showing that Kjeldahl’s method could not or not fully release N fixed by 2:1 minerals in soil, and therefore the determined results would not be the true “total N” for soils that contained large amount of the fixed N. The mineral fixedN averaged 166 mg kg-1, accounting for 10.1% of the total N while the residual fixed N amounted to 30.4 mg kg-1, equivalent to 1.9% of the total N or 18.3% of the total fixed N. The residual fixed N was correlated neither to organic matter nor to total N, but closely related to the total fixed N with a correlation coefficient of 0.598 (n=40), showing that the fixed N was the sole source of the residues. Soils having high residues of the fixed N were just those containing high fixed N, and soils containing high fixed N were just those containing high amount of 2:1 minerals. As a result, Kjeldahl’s method could not give a true value of the total N for such soils. However, for those containing small or little amount of 2:1 minerals, there was no significant difference in results measured by these methods.展开更多
Polarized spectra-hyperspectral data fusion technique was used to estimate the soluble sugar(SS),total nitrogen(N),and their ratio(SS/N),of greenhouse tomato leaves.Fresh tomato leaves of five different growth stages(...Polarized spectra-hyperspectral data fusion technique was used to estimate the soluble sugar(SS),total nitrogen(N),and their ratio(SS/N),of greenhouse tomato leaves.Fresh tomato leaves of five different growth stages(seedling,flowering,initial fruiting,mid-fruiting and picking stage)and five different nitrogen treatments(severe stress 25%,moderate stress 50%,mild stress 75%,normal 100%,and excess 150%)at every stage were collected for spectra acquisition and SS and N determination.Polarized reflectance spectra were acquired with a polarization reflectance spectrum spectro-goniophotometer system and four polarization degree features were extracted.Hyperspectral data were collected with a hyperspectral imaging system and four reflectance spectrum features and eight image features were extracted.Initially,models were built with polarization degree features,image features,and spectral features respectively.Linear and nonlinear fusion methods were comparatively used for modeling based on normalized data of the three sources.The results suggest that the performances of SS/N models are better than those of N and SS models,and the prediction capability of the Support Vector Machine(SVM)models of N and SS/N are superior to those obtained with single kind feature.This work indicates that the polarized spectrum-hyperspectral multidimensional information detecting method can feasibly judge the tomato nutrient stress conditions.Multi-features data fusion analysis technique can enhance the prediction accuracy of spectral diagnostics technology in precision agriculture.展开更多
A located field experiment was carried out to study the effects of different amount of chemical fertilizer usage on rice yield,economic benefits of rice,soil carbon(C) and total nitrogen(TN) under ploughing back o...A located field experiment was carried out to study the effects of different amount of chemical fertilizer usage on rice yield,economic benefits of rice,soil carbon(C) and total nitrogen(TN) under ploughing back of Chinese milk vetch for 5consecutive years.Six treatments were included in the experiment,they are CK(unfertilized),CF(100% chemical fertilizer with the amount of N,P2O5,K2 O being150,75,120 kg/hm^2respectively),A1(22 500 kg/hm^2 Chinese milk vetch and 100%chemical fertilizer),A2(Chinese milk vetch and 80% nitrogen and potassium fertilizer and 100% phosphate fertilizer),A3(Chinese milk vetch and 60% nitrogen and potassium fertilizer and 100% phosphate fertilizer),A4(Chinese milk vetch and 40% nitrogen and potassium fertilizer and 100% phosphate fertilizer).The results were as follows:application of fertilizer could increase the yield of rice,while Chinese milk vetch combined with fertilizer application had a much more increase effect in rice yield.Under the condition of milk vetch application with 22 500 kg/hm^2,the early rice yield of the treatment A1 was significantly increased by 7.7% compared with that of CF.And the yield of treatment A3 was basically identical to or slight increase in comparison with that of CF.Decreasing amount of fertilizers cloud improve output value of rice in the case of the utilization of Chinese milk vetch.The treatment A1 increased output value of rice by 5.92% in comparison of CF,and treatment A2 was by 4.08% in the next.Treatment A4 showed much better effect in increasing soil organic carbon and total nitrogen in the paddy soil than those of treatments applying mineral fertilizer only.There was a significant reduction on soil organic carbon and TN in treatment A2 in comparison with that of CF.In general,amount of application of milk vetch with 22 500 kg/hm^2 could replace chemical fertilizer partially,it also could improve rice yield,decrease the production cost,and raise the utilization efficiency of nutrients.展开更多
All stereoisomers of asperphenamate 1a and patriscabratine 2a were achieved with a high yield,and total synthesis of 2a is firstly described here.The absolute configuration of patriscabratine was determined as(S,S).Th...All stereoisomers of asperphenamate 1a and patriscabratine 2a were achieved with a high yield,and total synthesis of 2a is firstly described here.The absolute configuration of patriscabratine was determined as(S,S).The compounds 1a-d and 2a-d have been tested by MTT assay in T47D,MDA-MB231,HL60,Hela and SGC-7901 cell lines in vitro.Among them,the(R,S) stereoisomer shows the strongest anticancer effects,while the(S,R) shows the weakest one.展开更多
To verify the performance of the neutron total cross-sectional spectrometer, the neutron total cross section of carbon is initially measured in the energy range of 1 eV to 20 MeV using the time-of-flight method. The m...To verify the performance of the neutron total cross-sectional spectrometer, the neutron total cross section of carbon is initially measured in the energy range of 1 eV to 20 MeV using the time-of-flight method. The measurement is performed at the Back-n white neutron source with a 76-m time-of-flight path using the China Spallation Neutron Source. A multilayer fast fission chamber with 235U and 238U is employed as the neutron detector. The diameter and thickness of the natural graphite sample are 70 mm and 40 mm, respectively. Signal waveforms are collected using a data acquisition system. Off-line data processing was used to obtain the neutron time-of-flight spectra and transmissions. The uncertainty of the counting statistics is generally approximately 3% for each bin in the energy range of 1–20 MeV. It is determined that the results for the neutron total cross section of carbon obtained using ^235U cells are in good agreement with the results obtained using 238U cells within limits of statistical uncertainty. Moreover, the measured total cross sections show good agreement with the broadening evaluated data.展开更多
基金Supported by Public Project of Science and Technology Ministry(SYKYYW200903)The Ecological Carrying Capacity and Region Ecological Security Regulation of Northeast Industrial Base(2004CB418507)~~
文摘[Objective]The aim was to reveal the spatial distribution characteristics of total nutrients in soil and provide a theoretical basis for farmland management and improvement of crop yield. [Method]GIS technique was used to analyze the spatial distribution characteristics of total C,total N,total P and total K for different soil layers in Liaoning Province. [Result]The results showed that the content of total C,total N,total P decrease from east to west,but the content of total K was high in north district of Liaoning Province. The content of total C,total N,total P and total K was higher in soil surface (0-20 cm) than the lower (20-40 cm). Total K varied less with soil depth,and its mean content was respectively 17.64 g/kg and 17.08 g/kg for soil surface and soil lower layer. [Conclusion]The results of the distribution of soil total nutrients in different soil layers supplied a theory basis for farmland management.
基金Key Project of Chinese Academy of Sciences, No.ZKHZ-3-06
文摘Soil sediment samples of 10 layers with a spacing of 10 cm each were collected in different floodplain zones adjacent to Huolin River in the Xianghai Nature Reserve, and contents of total N, total P and organic matters were analyzed. The results showed that contents of total N, total P and organic matters were generally decreasing with the increase of distance from sample locations to the river channel, and contents of the three items were generally higher in the upper soil layer than that in the lower soil layer. The content variations displayed how flooding functions influenced nutrient matter content variations in floodplain soils since the flood inundation frequencies of the sample locations varied. The correlation analysis displayed that there were remarkable relativities between total N, total P and organic matters within definite spatial distance from the Huolin River channel.
文摘Soil organic carbon and nitrogen are used as indexes of soil quality assessment and sustainable land use management. At the same time, soil C/N ratio is a sensitive indicator of soil quality and for assessing the carbon and nitrogen nutrition balance of soils. We studied the characteristics of soil organic carbon and total nitrogen by investigating a large number of apple orchards in major apple production areas in China. High apple orchard soil organic carbon content was observed in the provinces of Heilongjiang, Xinjiang, and Yunnan, whereas low content was found in the provinces of Shandong, Henan, Hebei, and Shaanxi, with the values ranging between 6.44 and 7.76 g·kg-1. Similar to soil organic carbon, soil total nitrogen content also exhibited obvious differences in the 12 major apple producing provinces. Shandong apple orchard soil had the highest total nitrogen content (1.26 g·kg-1), followed by Beijing (1.23 g·kg-1). No significant difference was noted between these two regions, but their total nitrogen content was significantly higher than the other nine provinces, excluding Yunnan. The soil total nitrogen content for Xinjiang, Heilongjiang, Hebei, Henan, and Gansu was between 0.87 and 1.03 g·kg-1, which was significantly lower than that in Shandong and Beijing, but significantly higher than that in Liaoning, Shanxi, and Shaanxi. Six provinces exhibited apple orchard soil C/N ratio higher than 10, including Heilongjiang (15.42), Xinjiang (13.38), Ningxia (14.45), Liaoning (12.24), Yunnan (11.03), and Gansu (10.63). The soil C/N ratio was below 10 in the remaining six provinces, in which the highest was found in Shaanxi (9.47), followed by Beijing (8.98), Henan (7.99), and Shanxi (7.62), and the lowest was found in Hebei (6.80) and Shandong (6.05). Therefore, the improvement of soil organic carbon should be given more attention to increase the steady growth of soil C/N ratio.
基金supported by the Second Tibetan Plateau Scientific Expedition and Research Program(STEP2019QZKK0307)+1 种基金the Sichuan Science and Technology Program(2022YFS0500)the National Natural Science Foundation of China(No.41401313).
文摘The southeastern Tibetan Plateau,which profoundly affects East Asia by helping to maintain the stability of climate systems,biological diversity and clean water,is one of the regions most vulnerable to water erosion,wind erosion,tillage erosion,freeze-thaw erosion and overgrazing under global climate changes and intensive human activities.Spatial variations in soil erosion in terraced farmland(TL),sloping farmland(SL)and grassland(GL)were determined by the^(137)Cs tracing method and compared with spatial variations in soil organic carbon(SOC)and total nitrogen(total N).The^(137)Cs concentration in the GL was higher in the 0-0.03 m soil layer than in the other soil layers due to weak migration and diffusion under low precipitation and temperature conditions,while the^(137)Cs concentration in the soil layer of the SL was generally uniform in the 0-0.18 m soil layer due to tillage-induced mixing.Low^(137)Cs inventories appeared at the summit and toe slope positions in the SL due to soil loss by tillage erosion and water erosion,respectively,while the highest^(137)Cs inventories appeared at the middle slope posi-tions due to soil accumulation under relatively flat landform conditions.In the GL,the^(137)Cs data showed that higher soil erosion rates appeared at the summit due to freeze-thaw erosion and steep slope gradients and at the toe slope position due to wind erosion,gully erosion,freeze-thaw erosion and overgrazing.The^(137)Cs inventory generally increased from upper to lower slope positions within each terrace(except the lowest terrace).The^(137)Cs data along the terrace toposequence showed abrupt changes in soil erosion rates between the lower part of the upper terrace and the upper part of the immediate terrace over a short distance and net deposition on the lower and toe terraces.Hence,tillage erosion played an important role in the soil loss at the summit slope positions of each terrace,while water erosion dominantly transported soil from the upper terrace to the lower terrace and resulted in net soil deposition on the flat lower terrace.The SOC inventories showed similar spatial patterns to the^(137)Cs inventories in the SL,TL and GL,and significant correlations were found between the SOC and^(137)Cs inventories in these slope landscapes.The total N inventories showed similar spatial patterns to the inventories of,37Cs and SOC,and significant correlations were also found between the total N and^(137)Cs inventories in the SL,TL and GL Therefore,^(137)Cs can successfully be used for tracing soil,SOC and total N dynamics within slope landscapes in the southeastern Tibetan Plateau.
基金the National Natural Sci。ence Foundation of China(30230230,30070429 , 40201028)
文摘Forty soils from top layer (0-20 cm) were sampled in different regions of China and Kjeldahl, HF-Kjeldahl and double treatment methods were used to determine total N, total N plus fixed ammonium, and total N and the residual fixed ammonium left in soil after determination of total N, respectively, to evaluate if Kjeldahl’s method could include the fixed N by soil minerals. The fixed N by soil minerals was measured by Silva-Bremner procedure to make comparison. Results showed that total N determined by Kjeldahl’s method averaged 1.622 g kg-1, while that by HF- Kjeldahl’s method 1.633 g kg-1, and that by double procedure 1.666 g kg-1. Obviously results obtained by the last two methods, particularly the double treatment method, were higher than Kjeldahl’s, showing that Kjeldahl’s method could not or not fully release N fixed by 2:1 minerals in soil, and therefore the determined results would not be the true “total N” for soils that contained large amount of the fixed N. The mineral fixedN averaged 166 mg kg-1, accounting for 10.1% of the total N while the residual fixed N amounted to 30.4 mg kg-1, equivalent to 1.9% of the total N or 18.3% of the total fixed N. The residual fixed N was correlated neither to organic matter nor to total N, but closely related to the total fixed N with a correlation coefficient of 0.598 (n=40), showing that the fixed N was the sole source of the residues. Soils having high residues of the fixed N were just those containing high fixed N, and soils containing high fixed N were just those containing high amount of 2:1 minerals. As a result, Kjeldahl’s method could not give a true value of the total N for such soils. However, for those containing small or little amount of 2:1 minerals, there was no significant difference in results measured by these methods.
基金The authors are grateful to the financial support by China national key research and development plan(2017YFD0700504)China Postdoctoral Science Foundation(2016M601743)+3 种基金Senior talent research start-up fund of Jiangsu University(14JDG151)Natural Science Foundation of China Youth Fund(61901194)Natural Science Youth Foundation of Jiangsu Province(BK20180863,BK20180861)Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD-2018-87).
文摘Polarized spectra-hyperspectral data fusion technique was used to estimate the soluble sugar(SS),total nitrogen(N),and their ratio(SS/N),of greenhouse tomato leaves.Fresh tomato leaves of five different growth stages(seedling,flowering,initial fruiting,mid-fruiting and picking stage)and five different nitrogen treatments(severe stress 25%,moderate stress 50%,mild stress 75%,normal 100%,and excess 150%)at every stage were collected for spectra acquisition and SS and N determination.Polarized reflectance spectra were acquired with a polarization reflectance spectrum spectro-goniophotometer system and four polarization degree features were extracted.Hyperspectral data were collected with a hyperspectral imaging system and four reflectance spectrum features and eight image features were extracted.Initially,models were built with polarization degree features,image features,and spectral features respectively.Linear and nonlinear fusion methods were comparatively used for modeling based on normalized data of the three sources.The results suggest that the performances of SS/N models are better than those of N and SS models,and the prediction capability of the Support Vector Machine(SVM)models of N and SS/N are superior to those obtained with single kind feature.This work indicates that the polarized spectrum-hyperspectral multidimensional information detecting method can feasibly judge the tomato nutrient stress conditions.Multi-features data fusion analysis technique can enhance the prediction accuracy of spectral diagnostics technology in precision agriculture.
基金Supported by Special Fund for Agro-scientific Research in the Public Interest(201103005-08)National Science and Technology Support Program during the 12thFive-year Plan(2012BAD05B05-3)International Plant Nutrition Institute S&T Program(Hunan-16)~~
文摘A located field experiment was carried out to study the effects of different amount of chemical fertilizer usage on rice yield,economic benefits of rice,soil carbon(C) and total nitrogen(TN) under ploughing back of Chinese milk vetch for 5consecutive years.Six treatments were included in the experiment,they are CK(unfertilized),CF(100% chemical fertilizer with the amount of N,P2O5,K2 O being150,75,120 kg/hm^2respectively),A1(22 500 kg/hm^2 Chinese milk vetch and 100%chemical fertilizer),A2(Chinese milk vetch and 80% nitrogen and potassium fertilizer and 100% phosphate fertilizer),A3(Chinese milk vetch and 60% nitrogen and potassium fertilizer and 100% phosphate fertilizer),A4(Chinese milk vetch and 40% nitrogen and potassium fertilizer and 100% phosphate fertilizer).The results were as follows:application of fertilizer could increase the yield of rice,while Chinese milk vetch combined with fertilizer application had a much more increase effect in rice yield.Under the condition of milk vetch application with 22 500 kg/hm^2,the early rice yield of the treatment A1 was significantly increased by 7.7% compared with that of CF.And the yield of treatment A3 was basically identical to or slight increase in comparison with that of CF.Decreasing amount of fertilizers cloud improve output value of rice in the case of the utilization of Chinese milk vetch.The treatment A1 increased output value of rice by 5.92% in comparison of CF,and treatment A2 was by 4.08% in the next.Treatment A4 showed much better effect in increasing soil organic carbon and total nitrogen in the paddy soil than those of treatments applying mineral fertilizer only.There was a significant reduction on soil organic carbon and TN in treatment A2 in comparison with that of CF.In general,amount of application of milk vetch with 22 500 kg/hm^2 could replace chemical fertilizer partially,it also could improve rice yield,decrease the production cost,and raise the utilization efficiency of nutrients.
文摘All stereoisomers of asperphenamate 1a and patriscabratine 2a were achieved with a high yield,and total synthesis of 2a is firstly described here.The absolute configuration of patriscabratine was determined as(S,S).The compounds 1a-d and 2a-d have been tested by MTT assay in T47D,MDA-MB231,HL60,Hela and SGC-7901 cell lines in vitro.Among them,the(R,S) stereoisomer shows the strongest anticancer effects,while the(S,R) shows the weakest one.
基金supported by the National Key Research and Development Plan(No.2016YFA0401603)the National Natural Science Foundation of China(No.11675155)
文摘To verify the performance of the neutron total cross-sectional spectrometer, the neutron total cross section of carbon is initially measured in the energy range of 1 eV to 20 MeV using the time-of-flight method. The measurement is performed at the Back-n white neutron source with a 76-m time-of-flight path using the China Spallation Neutron Source. A multilayer fast fission chamber with 235U and 238U is employed as the neutron detector. The diameter and thickness of the natural graphite sample are 70 mm and 40 mm, respectively. Signal waveforms are collected using a data acquisition system. Off-line data processing was used to obtain the neutron time-of-flight spectra and transmissions. The uncertainty of the counting statistics is generally approximately 3% for each bin in the energy range of 1–20 MeV. It is determined that the results for the neutron total cross section of carbon obtained using ^235U cells are in good agreement with the results obtained using 238U cells within limits of statistical uncertainty. Moreover, the measured total cross sections show good agreement with the broadening evaluated data.