Patients with primary biliary cirrhosis have increased serum total antioxidant capacity measured with the crocin bleaching assay

AIM： The balance between oxidants and antioxidants can play an important role in the initiation and development of liver diseases. Recently, we have described a new automated method for the determination of total antioxidant capacity （TAC） in human serum and plasma.METHODS： We measured TAC and corrected TAC （CTACabstraction of interactions due to endogenous uric acid,bilirubin and albumin） in 52 patients with chronic liver diseases （41 patients with primary biliary cirrhosis （PBC）,10 patients with chronic hepatitis C and 13 patients with viral HCV cirrhosis） as well as in 10 healthy controls. In 23 PBC patients measurement were also done 6 mo after treatment with ursodeoxycholic acid （UDCA）. The TAC assay was based on a modification of the crocin bleaching assay. The results were correlated with routine laboratory measurements and the histological stage of PBC.RESULTS： There were no significant differences in TAC between the various groups. However, CTAC was considerably increased in the PBC group compared to controls and cirrhotics. Analysis of these patients according to disease stages showed that this increase was an early phenomenon observed only in stages I and II compared to controls, cirrhotics and patients with chronic hepatitis C）.After 6 mo of treatment with UDCA, levels of CTAC decreased to those similar to that of controls.CONCLUSION： Patients in the early stages of PBC present with high levels of corrected total antioxidant capacity and this maybe related to the pathophysiology of the disease. UDCA treatment restores the levels of CTAC to control levels.

Changes of total antioxidant capacity and total oxidant status of aqueous humor in diabetes patients and correlations with diabetic retinopathy

AIM:To measure changes of total oxidant status(TOS)and total antioxidant capacity(TAC) of aqueous humor(AH) in diabetic retinopathy(DR) patients,and to determine if there were any differences in TOS and TAC of AH in diabetic patients without retinopathy compared with non-diabetic patients.·METHODS:One hundred and three eyes of 103patients who were enrolled for cataract surgery were included in this study.Patients were grouped according to presence of diabetes and stage of DR.Prior to cataract surgery,0.1mL to 0.2mL of AH was aspirated and analyzed for TAC and TOS level using a colorimetric method.·RESULTS:TOS levels were highest among proliferative diabetic retinopathy(PDR) patients and lowest in patients with only cataracts.Results were statistically significant between all groups(P 【0.05).Whereas result between diabetic without retinopathy patients and non-proliferative diabetic retinopathy(NPDR) patients was not statistically significant(P =0.757).TAC levels were highest in patients with only cataract and lowest among PDR patients and results were statistically significant between all groups(P 【0.05).·CONCLUSION:Aqueous humor TAC levels are low in diabetic patients and reduced further in DR patients,TOS levels are increased in diabetic patients and this is exacerbated in DR patients.

The effect of fitness level on cardiac autonomic regulation, IL-6,total antioxidant capacity, and muscle damage responses to a single bout of high-intensity interval training

Purpose:The aim of this study was to investigate the influence of the cardiorespiratory fitness level on the response to high-intensity interval training(HIIT)with an individually adjusted running speed of the same relative intensity.The evaluation focused on acute cardiorespiratory response,postexercise cardiac autonomic modulation(heart rate variability(HRV))and biochemical markers of inflammation,oxidative stress,and muscle damage.Methods:Thirty participants were divided into 3 subgroups:well trained,moderately trained,and untrained.All the participants performed30 min HIIT composed of 6×2 min interval exercise with work-to-relief ratio=1 and work intensity 100%of individual velocity at maximal oxygen consumption(VO2max).Acute cardiorespiratory variables,postexercise HRV,lactate,interleukin-6(IL-6),total antioxidant capacity(TAC),creatine kinase,and myoglobin up to 4h after HIIT were monitored.Results:The differences in relatively expressed cardiorespiratory variables(heart rate,VO2)during HIIT were at most moderate,with the most pronounced between-group differences in absolute VO2 values.The disruption of the postexercise HRV was the most pronounced in untrained individuals,and this difference persisted 1 h after HIIT.The highest postexercise IL-6 and TAC concentrations and the lowest changes in creatine kinase and myoglobin were revealed in well-trained individuals.Conclusion:The higher fitness level was associated with the less pronounced postexercise cardiac autonomic changes and their faster restoration,even when there were similar acute cardiorespiratory responses.These findings were simultaneously accompanied by the higher postexercise IL-6 and TAC concentrations and less significant changes in muscle damage biochemical markers in well-trained individuals.

A Novel Assay for Measuring Total Antioxidant Capacity in Whole Blood and Other Biological Samples

A new method for rapidly determining total antioxidant capacity (TAC) in a biological sample has been devised and evaluated. A surfactant present in the reagent causes the lysis of cells in the sample. Glutathione, ascorbic acid, protein thiols and other main reducing substances in the sample react with a specific redox chromogen (2,6-dichlorophenolindophenol) and produce a change in the color of the reagent, which is determined photometrically at 630 nm. This new method quantifies the overall capacity of the sample to reduce a redox chromogen and it requires the minimum volumes and time. The assay can be used on a wide variety of biological samples including whole blood, serum, plasma, tissue extracts and homogenates, food, wine, fruit juice and other beverages. Results are expressed in terms of mmol/L glutathione equivalents. TAC can be assayed manually, on automatic analyzers or micro-plate readers.

Variability in total antioxidant capacity, antioxidant leaf pigments and foliage yield of vegetable amaranth

Twenty vegetable amaranth genotypes were evaluated for total antioxidant capacity, antioxidant leaf pigments, vitamins, and selection of suitable genotypes for extraction of juice in a randomized complete block design（RCBD） with three replications. Vegetable amaranth was rich in chlorophyll, β-cyanins, β-xanthins, betalains, carotene, ascorbic acid and total antioxidant. The genotypes VA14, VA16, VA18, VA15, and VA20 could be selected as amaranth vegtable varieties with high yields and abundance antioxidant leaf pigments and vitamins to produce juice. The genotypes VA13 and VA19 had above-average foliage yield and high antioxidant profiles while the genotypes VA2, VA3, VA9, VA11, VA12, and VA17 had a high antioxidant profiles and below-average foliage yield. These genotypes could be used as a donor parent for integration of potential high antioxidant profiles genes into other genotypes. The correlation study revealed a strong positive association among all the antioxidant leaf pigments, total antioxidant capacity and foliage yield. Selection based on total antioxidant capacity, antioxidant leaf pigments could economically viable to improve the yield potential of vegetable amaranth genotypes. Total carotene and ascorbic acid exhibited insignificant genotypic correlation with all the traits except total antioxidant capacity. This indicates that selection for antioxidant vitamins might be possible without compromising yield loss.

Effect of Colchicine on Total Antioxidant Capacity,Antioxidant Enzymes and Oxidative Stress Markers in Patients with Knee Osteoarthritis

Background/Aim: We aimed to investigate the effects of colchicine on clinical recovery, as well as oxidative stress markers and total antioxidant capacity (TAC) in whole blood of patients with knee osteoarthritis (OA). Materials and methods: Sixty patients with grade 2 - 3 knee OA according to ACR knee OA criteria whom examination of the knee joint synovial fluid by polarized light microscopy demonstrated CPDD crystals existence were included in the study. Fifty healthy subjects were included as a control group. Patients were divided randomly into two groups. The first group (paracetamol group) was given only paracetamol 3 gr daily p.o and the second group (colchicine and paracetamol group) was given colchicine 1,5 gr and paracetamol 3 gr daily p.o for six months. For outcome measures WOMAC and VAS were used. Superoxide dismutase (SOD), Catalase (CAT) ezyme activities and Glutathione (GSH) and Malondialdehyde (MDA) levels and TAC all were measured. Results: WOMAC scores were improved in both patient groups compared with pre-treatment evaluation (p 0.05). TAC was signifcantly increased only in colchicine/paracetamol group. Oxidant parameter MDA levels were significantly decreased in both paracetamol group and colchicine/paracetamol group. CAT, SOD enzyme activities and GSH levels did not change before and after treatment protocols in both patient groups. Conclusion: Both paracetamol 3000 mg/day and 3000 mg paracetamol plus 1,5 gr/day colchicine is effective in the treatment of patients with knee osteoarthritis. But the addition of colchicine to paracetamol produced significantly greater symptomatic benefit than paracetamol alone. Our study also showed that colchicine lowers whole blood MDA which is a lipid peroxidation compound and elevates TAC levels in patients with knee OA. This may show probable disease modifying effect of colchicine in knee OA which require further long period laboratory and radiologic investigations.

Effects of Heat Treatment on Flavonoids Content and Antioxidant Capacity of Buckwheat (Fagopyrum tataricum Gaert) Flour Ethanolic Extracts

The chemical composition, total flavonoids and antioxidant capacity of 50% ethanolic extracts from raw and cooked buckwheat flour from southwest of China were determined. The heat treatment used to gelatinize the flour starch significantly reduced the total flavonoids in the extract from 83.52 to 67.36 mg 100 g 1 of dry matter in the raw and cooked flour, respectively. The antioxidant capacity of the extracts was evaluated using autooxidation in a β carotene linoleate model system (AA or AAC), DPPH radical scavenging assay (IP) and oxygen radical absorbance capacity (ORAC) assay. Values for raw buckwheat flour extract were 97.84, 874.31, 76.45 and 94.92% for AA, AAC, ORAC and IP, respectively, while values in cooked buckwheat flour were 86.14, 842.88, 63.93 and 94.74% for AA, AAC, ORAC and IP, respectively. Values before cooked and raw buckwheat flour extracts were significantly different ( P <0.05) for AA, AAC, and ORAC.

Volatile components,total phenolic compounds,and antioxidant capacities of worm-infected Gomphidius rutilus

This study evaluated the effects of worm infection on the volatile components,total phenolic compounds,and antioxidant capacities of Gomphidius rutilus.G.rutilus without worms(GW),G.rutilus infected by a small amount of worms(GS;infected area<50%),and G.rutilus infected by a large amount of worms(GL;infected area>50%)were investigated.The volatile components of G.rutilus were analyzed by simultaneous distillation–extraction(SDE)and headspace solid-phase microextraction(HS-SPME)using gas chromatography–mass spectrometry(GC–MS).A total of 17 and 19 types of volatile compounds were detected,including ketones,alcohols,benzene,alkenes,aldehydes,esters,acids,and alkanes.Alcohols comprised the most abundant compound in GL,GS,and GW.The relative content of 1-octen-3-ol was the highest in all mushrooms.The concentration of eight-carbon(C8)compounds relative to the total volatile compounds varied widely,ranging from 40%(GW)to 64.34%(GS)and 84.42%(GS)and to 91.59%(GL),respectively,among the three samples.The antioxidant capability and the total phenolic contents of G.rutilus were evaluated in this study.The highest total phenolic content(TPC)of 192.23 mg GAE/g was found in GL,which differed significantly(P<0.05)from the latter two samples,whereas the lowest value of 156.11 mg GAE/g was found in GW.ABTS radical cation scavenging activity,FRAP ferric reducing antioxidant capacity(FRAP)radical scavenging activity,and oxygen radical absorbance capacity(ORAC)were investigated to screen the antioxidant properties of extracts.The contents of total phenolic compounds and their antioxidant capacities in vitro showed significant correlations(P<0.01).Among the three types of samples,the phenolic compounds of GL exhibited the highest antioxidant capacity,showing the values of 0.089 mM TE/g for ABTS,0.949 mM Fe^2+E/g for FRAP,and 1.952 M TE/g for ORAC.However,regarding the total antioxidant capacity,GS exhibited the highest antioxidant capacity,showing the values of 0.002648 mM TE/g for ABTS,0.004437 mM Fe^2+E/g for FRAP,and 0.256μM TE/g for ORAC.In conclusion,HS-SPME was more suitable for the extraction of volatile aroma components from G.rutilus.GL had the most abundant aroma components.GL had the highest TPC and antioxidant capacity compared with those of GS and GW,whereas GS showed the opposite results.Interestingly,GS was found to have the highest total antioxidant capacity in vitro.Based on these measured indicators,worm infection had no negative effect on the quality of G.rutilus.Therefore,worm-infected G.rutilus can also be consumed by humans.

Quantification of phenolic compounds and antioxidant capacity of an underutilized Indian fruit:Rayan[Manilkara hexandra(Roxb.)Dubard]

The fruit of Manilkara hexandra(Roxb.)Dubard is one of the most underutilized fruits of India and in Gujarat state.It is popularly known as‘Rayan’.The fruit and seed of Rayan were analysed for their total phenolic and flavonoid content,phenolic compounds and total antioxidant capacity with six different assay methods.The results indicated that the methanolic extract of Rayan fruit being a good source of phenolic(811.3 mg GAE/100 g fw)and flavonoid(485.56 mg RE/100 g fw)content.Also,eleven known phenolic compounds were tentatively identified for the first time from the fruit and seed of Rayan.The LC–MS/MS analysis of fruit revealed the presence of major phenolic compounds such as gallic acid,quercetin and kaempferol,while quercetin,gallic acid and vanillic acid in seed.The presence of quercetin suggests health benefits.The fruit of Rayan was also proved to be a better source of antioxidants as measured by FRAP,RPA,DPPHRSA,ABTSRSA and HRSA except NORSA in comparison with that of seed.The current study explains that M.hexandra is a relatively good source of antioxidants such as phenols and flavonoids for diet.

Dynamic Analysis of Nitric Oxide and Total Oxidant Capacity in Cow Uterine Secretion with Subclinical Endometritis

Subclinical endometritis is a physiological inflammation that serves to clear persistent contaminants from the uterus. To investigate the alteration of antioxidant, such as vitamin E（VE） and vitamin C（VC）, total oxidant capacity（TOC） and nitric oxide（NO） in cows with normal and subclinical endometritis（SCE）, we examined the concentrations of NO, VC and VE, TOC and polymorphonuclear neutrophils（PMN） percentage in uterine secretion. The cows were divided into two groups, normal（n=20） and subclinical endometritis（SCE, n=60）, based on endometrial cytology（presence of PMN≥5%）. Uterine secretion and blood were collected as described previously. Griess reaction was used to determine the concentration of NO. The concentrations of TOC, VC and VE were detected by a commercially available assay kit. The results showed that the concentrations of NO, TOC and PMN percentage were significantly higher（P〈0.01, P〈0.05, and P〈0.01, respectively） in uterine secretion with SCE compared to those from normal; however, the levels of VC and VE were significantly lesser（P〈0.01）. In conclusion, the concentrations of NO, TOC, VC, VE and PMN percentage differed between normal and SCE cows. Meanwhile, the relationship between the concentration of NO and PMN percentage from uterine secretion in cows with subclinical endometritis were positively correlated. Consequently, these alterations in NO, TOC, VC, VE levels and PMN percentage contributed to as a diagnostic index of the uterine inflammation, with the aim to increase the reproduction of the cows and the decrease economic losses.

Correlation between the Amplitude of Glucose Excursion and the Oxidative/Antioxidative System in Subjects with Different Types of Glucose Regulation

Objective To investigate effects of glucose excursion on the oxidative/antioxidative system in subjects with different types of glucose regulation. Methods A total of 30 individuals with normal glucose regulation (NGR), 27 subjects with impaired glucose regulation (IGR) and 27 subjects with newly diagnosed type 2 diabetes mellitus (T2DM) were selected and recruited for 3 days’ continuous glucose monitor system (CGMS) assessment. The data from CGMS was used to calculate the mean amplitude of glycemic excursion (MAGE), mean blood glucose (MBG) and its standard deviation (SDBG), area under the ROC curve when the blood glucose 5.6 mmol/L within 24 h (AUC 5.6), mean of daily differences (MODD), and mean postprandial glucose excursion (MPPGE). In all groups, the content or activity of malondialdehyde (MDA), total antioxidation capacity (TAOC) and glutathione peroxidase (GSH‐Px) were detected. Results Glucose excursion parameters of subjects with T2DM or IGR were higher than those of NGR subjects (P0.05 or 0.01). Moreover, Glucose excursion parameters of T2DM subjects were higher than those of IGR subjects (P0.05 or 0.01). Subjects with T2DM or IGR had significant higher MDA levels and lower GSH‐Px/MDA and TAOC/MDA levels compared to NGR subjects (P0.01). T2DM subjects had even higher MDA levels and lower GSH‐Px/MDA levels than IGR (P0.05 or 0.01). According to the median of normal population for MAGE, T2DM and IGR subjects were divided into MAGE2.6mmol/L Group and MAGE≤2.6mmol/L Group. MAGE2.6mmol/L Group had higher levels of MDA and lower levels of GSH‐Px/MDA than MAGE≤2.6mmol/L Group (P0.05). There was no significant difference between the two groups (P0.05) in terms of the levels of TAOC/MDA. Pearson correlation analysis showed that MDA was positively correlated with FPG, 2hPG, MAGE, and SBP. GSH‐Px/MDA was negatively correlated with MAGE and TC. TAOC/MDA was negatively correlated with FPG. Partial correlation analysis showed that the relationship between MDA and MAGE, GSH‐Px/MDA, and MAGE remained significant after adjustments for the other differences among groups. Conclusion Glucose excursion contributed significantly to promoting lipid peroxidation and decreasing antioxidation capacity than chronic sustained hyperglycemia did in the subjects with different types of glucose regulation.

Determination of Total Flavonoids and Its Antioxidant Ability in Houttuynia cordata

The total flavonoids of Houttuynia cordata are determined by UV-Vis spectrophotometry. The ex-traction condition of flavonoids from Houttuynia cordata includes: the extraction temperature is 70℃, solid-liquid ratio is 1:20, solvent is 80% ethanol, extraction time is 65 min. We studied the antioxidation of flavonoids in Houttuynia cordata, and results showed that flavonoids had strong reducing capacity, hydroxyl free radical scavenging activity and the superoxide anion radical scavenging ability, thus can provide a basis for the development of natural antioxidants.

Total Antioxidant Status and Other Markers to Distinguish Severely Obese Volunteers with and without Metabolic Syndrome

Obesity (Ob) is an accepted major risk factor for the metabolic syndrome (MetS), a combination of at least three of five risk factors, which predispose to high oxidative stress (OS), but all obese do not show symptoms of MetS. There is dearth of data comparing OS homeostasis of severely obese adults with and without MetS, and need for biomarkers to help in differential diagnosis. Erythrocytic lipid and protein damage markers, malondialdehyde (MDA) and protein carbonyl (PCO), antioxidant enzymes erythrocytic superoxide dismutase(SOD), catalase (CAT), plasma glutathione peroxidase (GPX), and total antioxidant capacity (TAC) as ferric-reducing-ability-of-plasma (FRAP) were compared to understand OS homeostasis among 102 severely Ob (body mass index > 30), 102 Ob with severe (z-score > 2) MetS as per National Cholesterol Education Program-Adult Treatment Panel III guidelines and 100 healthy non-obese Controls. MDA/PCO and all antioxidant enzymes were lowest for ObMetS, followed by Ob, indicating greater damage to protein moieties of the erythrocytic membrane. Multiple regression analysis confirmed z-scores > 2 as significant predictor of lowered enzymes and TAC. Receiver Operator Curve analysis predicted that TAC was the most potential biomarker for the diagnosis and prognosis of MetS with an Odds Ratio of 88.5 indicating the high probability that FRAP would be low for ObMetS (z-score > 2) than for Ob with BMI > 30, but z-scores < 1. TAC is qualified as the most effective biomarker to distinguish between severely obese respondents with and without metabolic syndrome, and as a useful candidate for study of homeostatic breakdown in metabolic syndrome and the importance of z-score in assessment of MetS in obese respondents.

Evaluation of the Chemical Profiling,Total Phenolic Composition,the Antioxidant and Antimicrobial Properties of the Essential Oils of Mentha piperita L.,Salvia officinalis L.,and Thymus vulgaris L.

The purpose of this study was to determine the chemical composition,total phenolic composition,the antioxidant and antimicrobial properties of the essential oils(EOs)of Mentha piperita L.,Salvia officinalis L.,and Thymus vulgaris L.The essential oils of M.piperita L.,S.officinalis L.,and T.vulgaris L.were analyzed by means of gas chromatography-mass spectrometry(GC-MS)to demonstrate their chemical composition.The antioxidant properties of the EOs were evaluated with the 2,2-diphenyl-picrylhydrazyl(DDPH)free radical scavenging assay,their total phenolic compound contents were determined by the Folin Ciocalteau method,and their antimicrobial activities were evaluated by the disc diffusion assay.The major compounds in the contents of the essential oils of M.piperita L.,S.officinalis L.,and T.vulgaris L.were found to be eucalyptol,1R-α-pinene,and o-cymene,respectively.In the 2,2-diphenyl-picrylhydrazyl(DDPH)assay,the EO of M.piperita L.(8,930.01μMTE/g)demonstrated the highest antioxidant activity,followed by the activities of the EOs of T.vulgaris L.(157.76μMTE/g)and S.officinalis L.(115.54μMTE/g).The total phenolic compound contents of M.piperita L.,T.vulgaris L.,and S.officinalis L.were measured as 135.074,0.242,and 0.221 mMGAE/g.All essential oils showed antioxidant activities and antimicrobial activities.The highest antimicrobial activity against S.aureus,A.nigeris,and C.albicans was determined in the EO of M.piperita L.within diameters of 42,32,and 28 mm,respectively.These properties of essential oils are used in the pharmaceutical and food industries.The essential oils are approved as official medications in many countries and take place in their pharmacopoeias。

产褥期感染危险因素分析及TAC、IFN-γ及α1-AG预测价值

目的:分析产褥期感染危险因素并探讨总抗氧化能力(TAC)、干扰素γ(IFN-γ)及α1酸性糖蛋白(α1-AG)预测价值。方法:回顾性收集2020年6月-2023年1月本院接诊的250例产妇临床资料,根据产褥期感染发生情况分为感染组(n=31)和未感染组(n=219),收集临床资料,分析产褥期感染危险因素,检测血清TAC、IFN-γ、α1-AG水平并采用受试者工作特征曲线(ROC)分析其预测产褥期感染价值。结果:单因素分析,两组年龄、孕周、产次、内分泌疾病比较无差异(P>0.05);两组产程、妇科炎症、妊娠高血压疾病、妊娠期糖尿病、产后出血、居住地、分娩方式、胎膜早破、卧床时间比较有差异(P<0.05);感染组血清IFN-γ(150.05±36.25 pg/ml)、α1-AG(156.35±40.91 mg/dl)高于未感染组(134.71±30.56 pg/ml、116.25±38.36 mg/dl),TAC(8.20±1.50 kU/ml)低于未感染组(10.83±2.45 kU/ml)(均P<0.05);白细胞计数、C反应蛋白及降钙素原水平产前两组无差异(P>0.05),产后两组均升高且感染组高于未感染组(P<0.05)。多因素非条件logistic分析,产程长、妇科炎症、妊娠高血压疾病、妊娠期糖尿病、产后出血、居住地为乡村、分娩方式为剖宫产、胎膜早破、卧床时间≥3d、TAC降低、IFN-γ升高及α1-AG升高均是产褥期感染发生的独立危险因素(P<0.05);ROC结果显示,预测产褥期感染血清TAC的曲线下面积(AUC)为0.808,灵敏度76.3%,特异度79.5,截断值9.56 kU/ml;血清IFN-γ的AUC为0.651,灵敏度61.5%,特异度69.4%,截断值141.24 pg/ml;血清α1-AG的AUC为0.753,灵敏度73.4%,特异度70.8%,截断值130.35 mg/dl。结论:产程长、妇科炎症、妊娠高血压疾病、妊娠期糖尿病、产后出血、居住地为乡村、剖宫产、胎膜早破、卧床时间长均是产褥期感染的独立危险因素,血清TAC、IFN-γ、α1-AG在产褥期感染者血清中表达异常且可对产褥期感染的发生有一定预测价值。

间苯三酚联合益气养阴汤治疗HDP对患者血清HMGB1、TAC、MDA水平及分娩结局影响

目的:分析益气养阴汤联合间苯三酚治疗妊娠期高血压(HDP)对患者血清高迁移率蛋白-1(HMGB1)、总抗氧化能力(TAC)、丙二醛(MDA)水平及分娩结局影响。方法:选取2021年1月-2022年12月本院收治的HDP患者96例,按随机数字表法分为对照组(48例,给予间苯三酚治疗)和联合组(48例,在对照组基础上给予益气养阴汤治疗)。比较两组疗效、血清HMGB1、TAC、MDA水平、脂代谢指标、血压、不良反应及分娩结局。结果:治疗后联合治疗组总有效率(95.8%)高于对照组(77.1%),两组舒张压、收缩压、平均动脉压均低于治疗前且联合组低于对照组,两组血清HMGB1、MDA水平均低于治疗前且联合组(1.85±0.47mg/L、23.56±3.56μmol/L)低于对照组(3.36±0.82 mg/L、32.47±5.16μmol/L),两组TAC水平均升高且联合组(11.26±2.65U/ml)高于对照组(9.24±2.03U/ml),两组甘油三酯、总胆固醇、低密度脂蛋白胆固醇水平均降低且联合组低于对照组,两组高密度脂蛋白胆固醇水平均上调且联合组高于对照组(均P<0.05)。联合组不良妊娠结局总发生率(8.3%)低于对照组(37.5%)(P=0.001),不良反应总发生率(6.3%)与对照组(12.5%)无差异(P=0.484)。结论:益气养阴汤联合间苯三酚治疗HDP,可更好控制患者血压水平,改善氧化应激和脂代谢水平,改善母婴结局,提高临床治疗效果,安全性好。

缺血性卒中患者血清尿酸水平与TAC、MDA及临床预后的相关性

目的探讨缺血性卒中患者血清尿酸水平与总抗氧化能力(total antioxidant capacity,TAC)、丙二醛(malondialdehyde,MDA)及临床预后的相关性。方法收集162例缺血性卒中患者的临床资料,检测血清尿酸、TAC、MDA水平。记录住院14d时改良Rankin评分(mRS>2分定义为预后不良,mRS≤2分为预后良好)。结果转归不良组糖尿病病史、既往卒中或短暂性脑缺血发作(transient ischemic stroke,TIA)病史发生率高于转归良好组,基线舒张压、尿酸、TAC、三酰甘油、高密度脂蛋白水平低于转归良好组,MDA和空腹血糖水平高于转归良好组,差异有统计学意义(P<0.05)。Logistic回归分析结果显示,既往卒中或TIA病史、MDA是缺血性脑卒中预后的独立危险因素;尿酸、高密度脂蛋白、TAC是预后的保护因素。相关分析结果显示,尿酸水平与TAC和预后呈正相关,与MDA呈负相关。结论尿酸水平较高的缺血性脑卒中患者氧化应激水平较低,临床预后较好。

依帕司他应用于早期糖尿病视网膜病变对MDA、SOD、TAC水平及内皮功能的保护研究

目的 研究依帕司他应用于早期糖尿病视网膜病变对血清丙二醛(MDA)、超氧化物歧化酶(SOD)、总抗氧化能力(TAC)水平及其内皮功能保护。方法 选取2016年6月~2018年8月在青岛市第八人民医院接受治疗的126例早期糖尿病视网膜患者,按照随机数表法分为观察组(63例)和对照组(63例)。对照组使用前列地尔注射液治疗,观察组在对照组基础上应用依帕司他辅助治疗。观察两组患者治疗后临床疗效;分析两组治疗前后血清MDA、SOD、TAC、糖化血红蛋白(HbA1c)水平变化和内皮功能各项参数变化;以及观察两组患者在治疗期间和治疗后不良反应发生情况。结果 治疗后,观察组总有效率(87.30%)明显高于对照组(73.02%),差异有统计学意义(P<0.05);观察者MDA水平明显低于对照组[(9.16±2.12)mol/L vs.(12.06±3.02)mol/L],SOD、TAC水平明显高于对照组[(94.16±9.88)U/L vs.(82.96±10.02)U/L;(20.98±3.88)Ku/L vs.(19.22±4.12)Ku/L],差异有统计学意义(P<0.05);观察组内皮功能参数NO、vWF、TM水平明低于对照组,差异有统计学意义(P<0.05);观察组患者Hb A1c水平明显明显低于对照组(6.97±1.13)%vs.(7.56±1.50)%(P<0.05)。结论 依帕司他应用于早期糖尿病视网膜病变能够有效提高临床疗效,显著降低MDA水平及并提高SOD、TAC水平,提高内皮功能保护能力,调节患者血糖水平。

糖尿病性白内障患者血清和泪液中SOD、LPO和TAC水平检测分析

目的探讨糖尿病性白内障患者血清和泪液中超氧化物歧化酶(superoxide dismutase,SOD)、过氧化脂质(lipid peroxide,LPO)、总抗氧化能力(total antioxidant capacity,TAC)等氧化应激指标的变化情况。方法通过采集患者和健康对照组中血清和泪液标本,采用酶联免疫吸附法检测样本中的SOD、LPO和TAC水平。结果糖尿病性白内障患者中血清和泪液中的SOD分别为(34.625±9.537)μU/L和(0.042±0.018)μU/L,均低于健康对照组中的(61.253±23.672)μU/L和(0.110±0.085)μU/L,差异均有统计学意义(t=18.256,13.514,P<0.05);血清及泪液LPO水平分别为(7.348±2.920)μmol/L和(1.423±0.481)μmol/L,高于对照组(5.125±1.376)μmol/L和(0.791±0.263)μmol/L,差异有统计学意义(t=9.624,14.924,P<0.05);患者血清和泪液中TAC分别为(10.124±2.191)kU/L和(5.032±1.424)kU/L,低于健康对照组中的(16.854±4.515)kU/L和(9.453±2.511)kU/L,差异有统计学意义(t=17.819,16.713,P<0.05)。结论糖尿病性白内障患者血清和泪液中SOD和TAC的水平下降,而LPO水平升高,氧化应激可能参与糖尿病性白内障发生发展进程。

HbA1c、TAC、FRU及SHBG联合诊断对妊娠期糖尿病早诊断的临床价值

目的糖化血糖蛋白(glycated glycemic protein,HbA1c)、总抗氧化能力(total antioxidant capacity,TAC)、果糖胺(fructosamine,FRU)及性激素结合球蛋白(sex hormone binding globulin,SHBG)联合诊断对妊娠期糖尿病早诊断的临床价值。方法随机选择2015年4月-2017年6月唐山市妇幼保健院产科行产前检查的192例孕妇。将其中确诊为妊娠期糖尿病孕妇56例列为研究对象(观察组),并根据患者意愿,将56例产妇分为治疗组和未治疗组。记录产妇HbA1c、TAC、FRU及SHBG的检测含量,并统计患者检测的准确率、灵敏度、特异性及阳性预测值。在妊娠后对新生儿状态进行随访,统计新生儿相关状态,分析诊断指标和新生儿状态之间相关性。结果观察组产妇的HbA1c、TAC、FRU及SHBG含量明显高于对照组,差异有统计学意义(P<0.05)。56例妊娠期糖尿病患者检测发现,HbA1c+TAC+FRU+SHBG联合检测的灵敏度、特异度、准确度及阳性预测值明显高于单项检测,差异有统计学意义(P<0.05)。治疗后,治疗组产妇的HbA1c、TAC、FRU及SHBG含量明显低于未治疗组,差异有统计学意义(P<0.05)。治疗组的新生儿不良反应发生率为7.14%,明显低于未治疗组新生儿不良反应发生率(17.85%),差异有统计学意义(P<0.05)。结论采用多指标联合诊断,能够提高妊娠期糖尿病的诊断特异性和敏感性,为临床早期干预提供依据,同时通过及时治疗,降低新生儿不良反应发生率,值得临床参考。