目的:观察番石榴叶总黄酮对脂肪乳剂所致高脂血症大鼠血脂、脂代谢酶及血液流变学的影响。方法:采用脂乳剂灌胃法建立大鼠高脂血症模型,将50只大鼠随机分为空白组、模型组、阿托伐他汀钙片(3.3 mg·kg^(-1))组、番石榴叶总黄酮高剂...目的:观察番石榴叶总黄酮对脂肪乳剂所致高脂血症大鼠血脂、脂代谢酶及血液流变学的影响。方法:采用脂乳剂灌胃法建立大鼠高脂血症模型,将50只大鼠随机分为空白组、模型组、阿托伐他汀钙片(3.3 mg·kg^(-1))组、番石榴叶总黄酮高剂量(200 mg·kg^(-1))组、番石榴叶总黄酮低剂量(100 mg·kg^(-1))组,每组各10只,连续给药6周。检测血清中三酰甘油(triglyceride,TG)、总胆固醇(triglyceride,TC)、高密度脂蛋白胆固醇(high density lipid protein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipid protein cholesterol,LDL-C)、超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、丙二醛(malondialdehyde,MDA)、总抗氧化能力(total antioxidative capability,T-AOC)、谷草转氨酶(aspartate aminotransferase,AST)、谷丙转氨酶(alanine transaminase,ALT)、碱性磷酸酶(alkaline phosphatase,ALP)水平,同时检测全血及血浆的血液流变学相关指标。结果:与模型组比较,番石榴叶总黄酮高剂量组、番石榴叶总黄酮低剂量组及阿托伐他汀钙片组均可不同程度降低大鼠血清中TC、TG、LDL-C、MDA、AST、ALT、ALP水平,升高HDL-C、SOD、GSX-PX、T-AOC水平,降低血液流变学各项指标(P<0.05)。结论:番石榴叶总黄酮可有效调节高脂血症大鼠血脂水平,提高脂代谢酶活性,改善血液流变学指标,有效防治高脂血症。展开更多
[Objectives]To study the protective effects and mechanism of POLYGONI PERFOLIATI HERBA on rats with nonalcoholic steatohepatitis( NASH). [Methods] Sixty rats were randomly divided into normal group,model group,metform...[Objectives]To study the protective effects and mechanism of POLYGONI PERFOLIATI HERBA on rats with nonalcoholic steatohepatitis( NASH). [Methods] Sixty rats were randomly divided into normal group,model group,metformin group( 0. 5 mg/kg),and high dose,medium dose,and low dose groups of total flavonoids of POLYGONI PERFOLIATI HERBA( 600,300,150 mg/kg). The standard feed was given to the normal group,and the model group and the total flavonoids groups were fed with high-fat diet for 13 weeks to establish the NASH rat model. At the 8 th week,the metformin group and the POLYGONI PERFOLIATI HERBA total flavonoids groups were given with the corresponding drug treatment for 6 weeks,blood was taken from the eyeball to collect liver tissue. Biochemical method was used to determine ALT,AST,TC,TG,HDL-c,LDL-c,SOD,MDA,GSH-Px activity or content in serum and HOMA-IR,and ELISA was used to measure the TNF-α,IL-1β and IL-6 content in liver tissue; Western blotting was used to detect the expression levels of AMPK,p-AMPK and ACC in liver tissue. [Results] Total flavonoids of POLYGONI PERFOLIATI HERBA could significantly decrease the activity or content of ALT,AST and MDA in serum of NASH rats( P < 0. 05,P < 0. 01),and enhance the activity of SOD and GSH-Px( P < 0. 05,P < 0. 01),reduce serum TC,TG,LDL-c levels and insulin resistance index,increase HDL-c levels( P < 0. 05,P < 0. 01); down-regulate liver IL-1β,IL-6,TNF-α,ACC levels and up-regulate p-AMPK expression( P < 0. 05,P < 0. 01). [Conclusions]The total flavonoids of POLYGONI PERFOLIATI HERBA have a good protective effect on NASH rats,and its mechanism may be related to the functions of regulating the lipid metabolism,alleviating insulin resistance,inhibiting oxidative stress,inhibiting inflammatory reaction and regulating AMPK and ACC protein expression.展开更多
文摘目的:观察番石榴叶总黄酮对脂肪乳剂所致高脂血症大鼠血脂、脂代谢酶及血液流变学的影响。方法:采用脂乳剂灌胃法建立大鼠高脂血症模型,将50只大鼠随机分为空白组、模型组、阿托伐他汀钙片(3.3 mg·kg^(-1))组、番石榴叶总黄酮高剂量(200 mg·kg^(-1))组、番石榴叶总黄酮低剂量(100 mg·kg^(-1))组,每组各10只,连续给药6周。检测血清中三酰甘油(triglyceride,TG)、总胆固醇(triglyceride,TC)、高密度脂蛋白胆固醇(high density lipid protein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipid protein cholesterol,LDL-C)、超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、丙二醛(malondialdehyde,MDA)、总抗氧化能力(total antioxidative capability,T-AOC)、谷草转氨酶(aspartate aminotransferase,AST)、谷丙转氨酶(alanine transaminase,ALT)、碱性磷酸酶(alkaline phosphatase,ALP)水平,同时检测全血及血浆的血液流变学相关指标。结果:与模型组比较,番石榴叶总黄酮高剂量组、番石榴叶总黄酮低剂量组及阿托伐他汀钙片组均可不同程度降低大鼠血清中TC、TG、LDL-C、MDA、AST、ALT、ALP水平,升高HDL-C、SOD、GSX-PX、T-AOC水平,降低血液流变学各项指标(P<0.05)。结论:番石榴叶总黄酮可有效调节高脂血症大鼠血脂水平,提高脂代谢酶活性,改善血液流变学指标,有效防治高脂血症。
基金Supported by Project of National Natural Science Foundation(81460602)Natural Science Foundation Project of Guangxi(2017GXNSFAA198326)
文摘[Objectives]To study the protective effects and mechanism of POLYGONI PERFOLIATI HERBA on rats with nonalcoholic steatohepatitis( NASH). [Methods] Sixty rats were randomly divided into normal group,model group,metformin group( 0. 5 mg/kg),and high dose,medium dose,and low dose groups of total flavonoids of POLYGONI PERFOLIATI HERBA( 600,300,150 mg/kg). The standard feed was given to the normal group,and the model group and the total flavonoids groups were fed with high-fat diet for 13 weeks to establish the NASH rat model. At the 8 th week,the metformin group and the POLYGONI PERFOLIATI HERBA total flavonoids groups were given with the corresponding drug treatment for 6 weeks,blood was taken from the eyeball to collect liver tissue. Biochemical method was used to determine ALT,AST,TC,TG,HDL-c,LDL-c,SOD,MDA,GSH-Px activity or content in serum and HOMA-IR,and ELISA was used to measure the TNF-α,IL-1β and IL-6 content in liver tissue; Western blotting was used to detect the expression levels of AMPK,p-AMPK and ACC in liver tissue. [Results] Total flavonoids of POLYGONI PERFOLIATI HERBA could significantly decrease the activity or content of ALT,AST and MDA in serum of NASH rats( P < 0. 05,P < 0. 01),and enhance the activity of SOD and GSH-Px( P < 0. 05,P < 0. 01),reduce serum TC,TG,LDL-c levels and insulin resistance index,increase HDL-c levels( P < 0. 05,P < 0. 01); down-regulate liver IL-1β,IL-6,TNF-α,ACC levels and up-regulate p-AMPK expression( P < 0. 05,P < 0. 01). [Conclusions]The total flavonoids of POLYGONI PERFOLIATI HERBA have a good protective effect on NASH rats,and its mechanism may be related to the functions of regulating the lipid metabolism,alleviating insulin resistance,inhibiting oxidative stress,inhibiting inflammatory reaction and regulating AMPK and ACC protein expression.
