Cornelian cherry is used in the food and pharmaceutical industry as an ornament, in traditional medicine, and in the manufacture of household items. It is widely used in medicine for the prevention and treatment...Cornelian cherry is used in the food and pharmaceutical industry as an ornament, in traditional medicine, and in the manufacture of household items. It is widely used in medicine for the prevention and treatment of many diseases. Therefore, it is important to research the chemical composition of these species. In the article, based on our research, the analysis of the quantitative calculation of flavonoids and amino acids of the fruits of the “Elegant” and “Svetlyachok” cherry varieties was carried out. It was found that the amount of amino acids in the fruits of Cornus mas L., introduced in Uzbekistan, is higher in the variety “Svetlyachok” 2.643235 mg/g. In “Elegant” variety it was 1.794235 mg/g. The amount of 4 different flavonoids in the fruit was also determined. It has been established that the Elegant variety has a high concentration of lutein and rutin, and the svetlyachok has a high concentration of apigenin and quercetin.展开更多
[Objectives]To explore the process of simultaneous extracting total flavonoids and chlorogenic acid from Flos Lonicerae by pressure assisted extraction.[Methods]Based on the single factor experiment,orthogonal experim...[Objectives]To explore the process of simultaneous extracting total flavonoids and chlorogenic acid from Flos Lonicerae by pressure assisted extraction.[Methods]Based on the single factor experiment,orthogonal experiments were carried out to investigate the effects of solvent concentration,solid-to-liquid ratio,extraction pressure,extraction time and extraction temperature on the extraction rate of total flavonoids and chlorogenic acid.[Results]The results showed that the extraction pressure had a significant effect on the extraction of two kinds of chemical constituents from F.Lonicerae.The optimum process conditions were as follows:solvent concentration was 60%,solid-liquid ratio was 1∶20(g/mL),extraction pressure was 2 MPa,extraction time was 30 min,extraction temperature was 70℃.The process conditions are stable,and the extraction efficiency is higher than that of ultrasonic assisted extraction method.Under the test conditions,the extraction rates of total flavonoids and chlorogenic acids were respectively 15.66%and 3.89%.[Conclusions]This study provides a new theoretical basis for the development and utilization of F.Lonicerae.展开更多
Background:Reduced protein diet manifested potential to mitigate heat production based on the concept of ideal amino acid profile.The hypothesis of this study was that lactating sows fed a low crude protein(LCP)diet w...Background:Reduced protein diet manifested potential to mitigate heat production based on the concept of ideal amino acid profile.The hypothesis of this study was that lactating sows fed a low crude protein(LCP)diet with supplemental amino acid produce less heat compared to those fed a high crude protein(HCP)diet under both thermal neutral(TN)and heat stress(HS)conditions.Methods:Thirty-two lactating sows were allotted to HCP(193 g CP/kg)and LCP(140 g CP/kg)diets under thermal neutral(TN,21±1.5°C)or cycling heat stress(HS,32±1.5°C daytime and 24±1.5°C nighttime)conditions.Diets contained 0.90%SID lysine and 10.8 MJ/kg net energy.Positive pressure indirect calorimeters were used to measure gas exchange in individual sows with litters,and individual piglets on days 4,8,14 and 18.Sow and litter weights were recorded on days 1,10 and 21.Results:Sow total heat production(THP)was calculated by subtracting litter THP from sow+litter THP based on BW0.75.Sow BW and body protein(BP)loss was greater for LCP diet compared to HCP diet in peak lactation(P<0.05 and P<0.01,respectively)and throughout the entire lactation period(P<0.05 and P=0.056,respectively)under HS conditions.Heat-stressed sows fed HCP diet had higher(P<0.05)rectal temperature at 13:00(P<0.05)and 19:00(P<0.01),and higher respiration rate at 07:00(P<0.05),13:00(P<0.05)and 19:00(P<0.05)compared to TN sows fed HCP diet.In sows fed LCP diet,those under HS tended to have higher(P=0.098)rectal temperature at 13:00 and had higher(P<0.05)respiration rate at 07:00,13:00 and 19:00 compared to TN sows.The relationship between daily THP and days in lactation of sows fed LCP diet was quadratic(P<0.05),with an ascending trend until day 14 and a descending trend from days 14 to 18.Sows fed LCP diet had lower daily THP at day 18(P<0.001)compared to those fed the HCP diet under HS conditions.Conclusion:Reduction in THP in sows fed LCP diet was largely associated with THP on day 18 of lactation under HS conditions.Feeding LCP diets alleviated the increased body temperature in sows under HS conditions throughout lactation,which was accompanied by a reduction in respiration rate.Total heat production is associated with days in lactation,in particular under HS conditions with THP appearing to peak between days 14 and 18.展开更多
Objective:The objective was to study the effect of total flavonoids of Herba Taxilli(TFHT)on osteoporotic(OP)rats induced by retinoic acid(RA)and explore its mechanism.Methods:Three-month-old female Sprague Dawley rat...Objective:The objective was to study the effect of total flavonoids of Herba Taxilli(TFHT)on osteoporotic(OP)rats induced by retinoic acid(RA)and explore its mechanism.Methods:Three-month-old female Sprague Dawley rats were randomly divided into six groups,each with ten rats,i.e.normal control group,model group,and positive control group and three TFHT groups(at high-,middle-,and low-doses).Except the normal control group,rats of all the other groups were administered RA by gavage(70 mg/kg/day)every morning for 14 days to establish an OP model;at the same time,all the rats were given Corresponding therapeutic drug by gavage for 28 days:rats in the three TFHT groups were given TFHT(400,200,and 100 mg/kg/day,respectively)and rats in the positive control group were given Long mu Zhuang gu Granule 300 mg/kg/day.Rats in the model group and normal group were given an equal volume of 1%sodium carboxymethyl cellulose sodium suspension.After the experiment,blood was taken to measure the levels of serum calcium(Ca^(2+)),alkaline phosphatase(ALP),and tartrate-resistant acid phosphatase(TRAP);the spleen,uterus,and ovary were dissected to determine the organ coefficient;the left hind limb femur was dissected to determine bone mineral density(BMD),bone dry weight(BDW),bone wet weight(BWW),bone diameter(BD),bone length(BL),bone dry weight/rat weight(BDW/W),bone wet weight/rat weight(BWW/W),bone dry weight/bone wet weight(BDW/BWW),bone dry weight/bone length(BDW/L),and bone wet weight/bone diameter(BWW/D);and the right hind limb femur was dissected to observe the bone path morphological changes.Results:Compared with the model group,the serum Ca^(2+)content,uterus plus ovary coefficient,BMD,BDW,BDW/W,BWW/W,BDW/BWW,BDW/L,and BWW/D in the TFHT groups increased,whereas the spleen coefficient,ALP,and TRAP decreased in the TFHT groups,and the differences were statistically significant(P<0.05 or P<0.01).Conclusion:TFHT was found effective in treating OP in rats induced by RA;the main mechanisms were increasing the serum Ca^(2+)content and reducing the level of ALP and TRAP.展开更多
Rosa roxburghii Tratt., is an important commercial horticultural crop in China due to its nutritional and medicinal value. To date, the economic production of a single predominant cultivar of R. roxburghii(‘Guinong 5...Rosa roxburghii Tratt., is an important commercial horticultural crop in China due to its nutritional and medicinal value. To date, the economic production of a single predominant cultivar of R. roxburghii(‘Guinong 5') consumes 30 000 hm^2 in China. Here, we characterize the amino acid composition of ‘Guinong 5' and two novel genotypes(AL11, GP6) that prove valuable and deserve wide application. Here, 17 amino acids(acid hydrolysis of proteins) were detected in ‘Guinong 5', AL11 and GP6 fruits, with Glutamine and Asparagine being abundant, and L-cystine being absent. The essential amino acids and total amino acids were the highest in AL11, while Asparagine was most abundant in GP6. For the free amino acid, 18 amino acids and 9 metabolites were detected in ‘Guinong 5', AL11 and GP6 fruits. γ-aminobutyric acid, L-arginine and ornithine were the most abundant in ‘Guinong 5', while phosphoserine and taurine were the highest in AL11. In comparison, GP6 had the highest levels of L-asparagine, an essential amino acid, and the highest total N containing compounds.展开更多
Knowledge of the amounts and digestibility of amino acids in pig feedstuffs is essential for calculating the appropriate inclusion level in a complete diet.Wet chemical analysis and in vivo digestibility trials are ti...Knowledge of the amounts and digestibility of amino acids in pig feedstuffs is essential for calculating the appropriate inclusion level in a complete diet.Wet chemical analysis and in vivo digestibility trials are time-consuming and costly and cannot be used for routine assessment.Near-infrared spectroscopy(NIRS)offers a rapid,cost effective and environmentally friendly method for evaluating feedstuffs.Calibrations models were developed using NIRS to predict the content of crude protein and 18 amino acids from a wide range of feedstuffs used in pig production(n=607).The samples ranged from single feed ingredients(containing amino acids from 0.3 to 129.8 g/kg of dry matter)to feed mixtures(containing amino acids from 1.2 to 53.2 g/kg of dry matter).The predictive ability of the calibrations was tested with an independent dataset(n=150)and with cross-validation.Furthermore,we compare these calibrations with calibrations developed on more narrowly defined groups of samples and with predictions from regression analysis of crude protein.The models were able to predict the concentrations of crude protein and 18 amino acids with good levels of precision and high coefficients of determination for calibration(RSQ^(CAL))from 0.91 to 0.99 and validation(RSQ^(VAL))from 0.87 to 0.97.Calibration models were able to predict all amino acids except tryptophan and valine with greater accuracy than those from protein regression.We also developed calibration models to predict the apparent ileal and total tract digestibility of protein and amino acids.With the exception of tryptophan,RSQ values(>0.7)and standard error of cross validation(SECV)values(<5%)were obtained for the digestibility of most of the amino acids.In conclusion,NIRS can be used to predict crude protein and amino acid concentrations from a wide range of single ingredients and feed mixtures used for pig diets without separate models for each feedstuff.The digestibility of protein and amino acids can be predicted with an acceptable accuracy to be useful in formulating pig diets.展开更多
The purpose of this paper was to identify content changes in the main nutritional indicators of three common leafy vegetables, and to provide a theoretical basis for the protection of leafy vegetables from acid rain. ...The purpose of this paper was to identify content changes in the main nutritional indicators of three common leafy vegetables, and to provide a theoretical basis for the protection of leafy vegetables from acid rain. The experiment investigated the effects of simulated acid rain on four main nutritional indicators, including soluble sugar, total free amino acid, soluble protein and vitamin C during the application of simulated acid rain(SAR) in pakchoi(Brassica rapa chinensis), rape(Brassica campestris L.) and lettuce(Lactuca sativa Linn. var. ramosa Hort). The vegetables were respectively exposed to SAR of pH=7.0, 5.6, 5.0, 4.0, 3.0 and a control level of pH=6.5. The concentrations of the four main nutritional indicators were determined at harvest. The results show that nutritional quality of the three leafy vegetable species decreased with the declining of pH values of SAR. The higher the acidity of SAR was, the more significant the inhibitions were. Nutritional quality of lettuce was the most affected by simulated acid rain, followed by pakchoi and rape. The change range of soluble protein content was higher than those of the other three indicators’ contents, which indicates that soluble protein is most sensitive to simulated acid rain.展开更多
文摘Cornelian cherry is used in the food and pharmaceutical industry as an ornament, in traditional medicine, and in the manufacture of household items. It is widely used in medicine for the prevention and treatment of many diseases. Therefore, it is important to research the chemical composition of these species. In the article, based on our research, the analysis of the quantitative calculation of flavonoids and amino acids of the fruits of the “Elegant” and “Svetlyachok” cherry varieties was carried out. It was found that the amount of amino acids in the fruits of Cornus mas L., introduced in Uzbekistan, is higher in the variety “Svetlyachok” 2.643235 mg/g. In “Elegant” variety it was 1.794235 mg/g. The amount of 4 different flavonoids in the fruit was also determined. It has been established that the Elegant variety has a high concentration of lutein and rutin, and the svetlyachok has a high concentration of apigenin and quercetin.
基金Supported by Scientific Research Project of Hunan Provincial Department of Education in 2017(17C1603)
文摘[Objectives]To explore the process of simultaneous extracting total flavonoids and chlorogenic acid from Flos Lonicerae by pressure assisted extraction.[Methods]Based on the single factor experiment,orthogonal experiments were carried out to investigate the effects of solvent concentration,solid-to-liquid ratio,extraction pressure,extraction time and extraction temperature on the extraction rate of total flavonoids and chlorogenic acid.[Results]The results showed that the extraction pressure had a significant effect on the extraction of two kinds of chemical constituents from F.Lonicerae.The optimum process conditions were as follows:solvent concentration was 60%,solid-liquid ratio was 1∶20(g/mL),extraction pressure was 2 MPa,extraction time was 30 min,extraction temperature was 70℃.The process conditions are stable,and the extraction efficiency is higher than that of ultrasonic assisted extraction method.Under the test conditions,the extraction rates of total flavonoids and chlorogenic acids were respectively 15.66%and 3.89%.[Conclusions]This study provides a new theoretical basis for the development and utilization of F.Lonicerae.
基金supported by the USDA-NIFA(award number 2014–67015-21832).
文摘Background:Reduced protein diet manifested potential to mitigate heat production based on the concept of ideal amino acid profile.The hypothesis of this study was that lactating sows fed a low crude protein(LCP)diet with supplemental amino acid produce less heat compared to those fed a high crude protein(HCP)diet under both thermal neutral(TN)and heat stress(HS)conditions.Methods:Thirty-two lactating sows were allotted to HCP(193 g CP/kg)and LCP(140 g CP/kg)diets under thermal neutral(TN,21±1.5°C)or cycling heat stress(HS,32±1.5°C daytime and 24±1.5°C nighttime)conditions.Diets contained 0.90%SID lysine and 10.8 MJ/kg net energy.Positive pressure indirect calorimeters were used to measure gas exchange in individual sows with litters,and individual piglets on days 4,8,14 and 18.Sow and litter weights were recorded on days 1,10 and 21.Results:Sow total heat production(THP)was calculated by subtracting litter THP from sow+litter THP based on BW0.75.Sow BW and body protein(BP)loss was greater for LCP diet compared to HCP diet in peak lactation(P<0.05 and P<0.01,respectively)and throughout the entire lactation period(P<0.05 and P=0.056,respectively)under HS conditions.Heat-stressed sows fed HCP diet had higher(P<0.05)rectal temperature at 13:00(P<0.05)and 19:00(P<0.01),and higher respiration rate at 07:00(P<0.05),13:00(P<0.05)and 19:00(P<0.05)compared to TN sows fed HCP diet.In sows fed LCP diet,those under HS tended to have higher(P=0.098)rectal temperature at 13:00 and had higher(P<0.05)respiration rate at 07:00,13:00 and 19:00 compared to TN sows.The relationship between daily THP and days in lactation of sows fed LCP diet was quadratic(P<0.05),with an ascending trend until day 14 and a descending trend from days 14 to 18.Sows fed LCP diet had lower daily THP at day 18(P<0.001)compared to those fed the HCP diet under HS conditions.Conclusion:Reduction in THP in sows fed LCP diet was largely associated with THP on day 18 of lactation under HS conditions.Feeding LCP diets alleviated the increased body temperature in sows under HS conditions throughout lactation,which was accompanied by a reduction in respiration rate.Total heat production is associated with days in lactation,in particular under HS conditions with THP appearing to peak between days 14 and 18.
文摘Objective:The objective was to study the effect of total flavonoids of Herba Taxilli(TFHT)on osteoporotic(OP)rats induced by retinoic acid(RA)and explore its mechanism.Methods:Three-month-old female Sprague Dawley rats were randomly divided into six groups,each with ten rats,i.e.normal control group,model group,and positive control group and three TFHT groups(at high-,middle-,and low-doses).Except the normal control group,rats of all the other groups were administered RA by gavage(70 mg/kg/day)every morning for 14 days to establish an OP model;at the same time,all the rats were given Corresponding therapeutic drug by gavage for 28 days:rats in the three TFHT groups were given TFHT(400,200,and 100 mg/kg/day,respectively)and rats in the positive control group were given Long mu Zhuang gu Granule 300 mg/kg/day.Rats in the model group and normal group were given an equal volume of 1%sodium carboxymethyl cellulose sodium suspension.After the experiment,blood was taken to measure the levels of serum calcium(Ca^(2+)),alkaline phosphatase(ALP),and tartrate-resistant acid phosphatase(TRAP);the spleen,uterus,and ovary were dissected to determine the organ coefficient;the left hind limb femur was dissected to determine bone mineral density(BMD),bone dry weight(BDW),bone wet weight(BWW),bone diameter(BD),bone length(BL),bone dry weight/rat weight(BDW/W),bone wet weight/rat weight(BWW/W),bone dry weight/bone wet weight(BDW/BWW),bone dry weight/bone length(BDW/L),and bone wet weight/bone diameter(BWW/D);and the right hind limb femur was dissected to observe the bone path morphological changes.Results:Compared with the model group,the serum Ca^(2+)content,uterus plus ovary coefficient,BMD,BDW,BDW/W,BWW/W,BDW/BWW,BDW/L,and BWW/D in the TFHT groups increased,whereas the spleen coefficient,ALP,and TRAP decreased in the TFHT groups,and the differences were statistically significant(P<0.05 or P<0.01).Conclusion:TFHT was found effective in treating OP in rats induced by RA;the main mechanisms were increasing the serum Ca^(2+)content and reducing the level of ALP and TRAP.
基金the National Natural Science Foundation of China(31660549)the High-level Innovative Talents Training Plan in Guizhou Province(20164016)Science and Technology Plan Projects in Guizhou Province(20153026-1)
文摘Rosa roxburghii Tratt., is an important commercial horticultural crop in China due to its nutritional and medicinal value. To date, the economic production of a single predominant cultivar of R. roxburghii(‘Guinong 5') consumes 30 000 hm^2 in China. Here, we characterize the amino acid composition of ‘Guinong 5' and two novel genotypes(AL11, GP6) that prove valuable and deserve wide application. Here, 17 amino acids(acid hydrolysis of proteins) were detected in ‘Guinong 5', AL11 and GP6 fruits, with Glutamine and Asparagine being abundant, and L-cystine being absent. The essential amino acids and total amino acids were the highest in AL11, while Asparagine was most abundant in GP6. For the free amino acid, 18 amino acids and 9 metabolites were detected in ‘Guinong 5', AL11 and GP6 fruits. γ-aminobutyric acid, L-arginine and ornithine were the most abundant in ‘Guinong 5', while phosphoserine and taurine were the highest in AL11. In comparison, GP6 had the highest levels of L-asparagine, an essential amino acid, and the highest total N containing compounds.
基金the Feed-a-Gene Project and has received funding from the European Union’s H2020 Program under grant agreement no 633531The funding body had no role in the design of the study and collection,analysis,and interpretation of data or in writing the manuscrip。
文摘Knowledge of the amounts and digestibility of amino acids in pig feedstuffs is essential for calculating the appropriate inclusion level in a complete diet.Wet chemical analysis and in vivo digestibility trials are time-consuming and costly and cannot be used for routine assessment.Near-infrared spectroscopy(NIRS)offers a rapid,cost effective and environmentally friendly method for evaluating feedstuffs.Calibrations models were developed using NIRS to predict the content of crude protein and 18 amino acids from a wide range of feedstuffs used in pig production(n=607).The samples ranged from single feed ingredients(containing amino acids from 0.3 to 129.8 g/kg of dry matter)to feed mixtures(containing amino acids from 1.2 to 53.2 g/kg of dry matter).The predictive ability of the calibrations was tested with an independent dataset(n=150)and with cross-validation.Furthermore,we compare these calibrations with calibrations developed on more narrowly defined groups of samples and with predictions from regression analysis of crude protein.The models were able to predict the concentrations of crude protein and 18 amino acids with good levels of precision and high coefficients of determination for calibration(RSQ^(CAL))from 0.91 to 0.99 and validation(RSQ^(VAL))from 0.87 to 0.97.Calibration models were able to predict all amino acids except tryptophan and valine with greater accuracy than those from protein regression.We also developed calibration models to predict the apparent ileal and total tract digestibility of protein and amino acids.With the exception of tryptophan,RSQ values(>0.7)and standard error of cross validation(SECV)values(<5%)were obtained for the digestibility of most of the amino acids.In conclusion,NIRS can be used to predict crude protein and amino acid concentrations from a wide range of single ingredients and feed mixtures used for pig diets without separate models for each feedstuff.The digestibility of protein and amino acids can be predicted with an acceptable accuracy to be useful in formulating pig diets.
基金Supported by the National Basic Research Program of China(No.2005CB422207)
文摘The purpose of this paper was to identify content changes in the main nutritional indicators of three common leafy vegetables, and to provide a theoretical basis for the protection of leafy vegetables from acid rain. The experiment investigated the effects of simulated acid rain on four main nutritional indicators, including soluble sugar, total free amino acid, soluble protein and vitamin C during the application of simulated acid rain(SAR) in pakchoi(Brassica rapa chinensis), rape(Brassica campestris L.) and lettuce(Lactuca sativa Linn. var. ramosa Hort). The vegetables were respectively exposed to SAR of pH=7.0, 5.6, 5.0, 4.0, 3.0 and a control level of pH=6.5. The concentrations of the four main nutritional indicators were determined at harvest. The results show that nutritional quality of the three leafy vegetable species decreased with the declining of pH values of SAR. The higher the acidity of SAR was, the more significant the inhibitions were. Nutritional quality of lettuce was the most affected by simulated acid rain, followed by pakchoi and rape. The change range of soluble protein content was higher than those of the other three indicators’ contents, which indicates that soluble protein is most sensitive to simulated acid rain.