Objective:To explore and validate the potential targets of Paeoniae Radix Alba(P.Radix,Bai Shao)in protecting against chemical liver injury through network pharmacology,molecular docking technology,and in vitro cell e...Objective:To explore and validate the potential targets of Paeoniae Radix Alba(P.Radix,Bai Shao)in protecting against chemical liver injury through network pharmacology,molecular docking technology,and in vitro cell experiments.Methods:Network pharmacology was used to identify the common potential targets of P.Radix and chemical liver injury.Molecular docking was used to fit the components,which were subsequently verified in vitro.A cell model of hepatic fibrosis was established by activating hepatic stellate cell(HSC)-LX2 cells with 10 ng/mL transforming growth factor-β1.The cells were exposed to different concentrations of total glucosides of paeony(TGP),the active substance of P.Radix,and then evaluated using the cell counting kit-8 assay,enzyme-linked immunosorbent assay,and western blot.Results:Analysis through network pharmacology revealed 13 key compounds of P.Radix,and the potential targets for preventing chemical liver injury were IL-6,AKT serine/threonine kinase 1,jun protooncogene,heat shock protein 90 alpha family class A member 1(HSP90AA1),peroxisome proliferator activated receptor gamma(PPARG),PTGS2,and CASP3.Gene Ontology(GO)enrichment analysis indicated the involvement of response to drugs,membrane rafts,and peptide binding.Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis revealed that the main pathways involved lipid and atherosclerosis and chemical carcinogenesis-receptor activation.Paeoniflorin and albiflorin exhibited strong affinity for HSP90AA1,PTGS2,PPARG,and CASP3.Different concentrations of TGP can inhibit the expression of COL-I,COL-III,IL-6,TNF-a,IL-1β,HSP-90a,and PTGS2 while increasing the expression of PPAR-γand CASP3 in activated HSC-LX2 cells.Conclusion:P.Radix primarily can regulate targets such as HSP90AA1,PTGS2,PPARG,CASP3.TGP,the main active compound of P.Radix,protects against chemical liver injury by reducing the inflammatory response,activating apoptotic proteins,and promoting the apoptosis of activated HSCs.展开更多
目的 白芍中药产品种类繁多,为提高白芍的质量控制水平,建立同时测定白芍药材及制剂中9个成分的一测多评(quantitative analysis of multi-components with a single-marker, QAMS)方法。方法 采用高效液相色谱(HPLC)法,以芍药苷为参照...目的 白芍中药产品种类繁多,为提高白芍的质量控制水平,建立同时测定白芍药材及制剂中9个成分的一测多评(quantitative analysis of multi-components with a single-marker, QAMS)方法。方法 采用高效液相色谱(HPLC)法,以芍药苷为参照物,计算其他8种成分没食子酸、羟基芍药苷、儿茶素、芍药内酯苷、没食子酰芍药苷、五没食子酰葡萄糖、苯甲酸和苯甲酰芍药苷的相对校正因子(fk/s),并对fk/s进行耐用性考察。收集18批不同产地来源的白芍药材及其炮制品以及不同批号的白芍总苷胶囊,分别采用外标法和QAMS法测定9个成分含量,比较两者的差异,验证QAMS法的可行性和准确性。结果 各个成分的fk/s重复性良好,对18批样品运用QAMS法和外标法检测得到的各成分含量无显著性差异(RSD<4%);不同产地来源及炮制品的白芍药材中9个成分含量均存在较大差异,说明应提高白芍中药的质量控制标准;不同批次间白芍总苷胶囊的主要成分含量差异不大,说明制剂工艺相对稳定。结论 研究所建立的QAMS法准确性高,可用于白芍药材及其相关制剂的多成分同时定量分析,为白芍中药的全面质量控制提供方法参考。展开更多
基金supported by the National Natural Science Foundation of China(82074036).
文摘Objective:To explore and validate the potential targets of Paeoniae Radix Alba(P.Radix,Bai Shao)in protecting against chemical liver injury through network pharmacology,molecular docking technology,and in vitro cell experiments.Methods:Network pharmacology was used to identify the common potential targets of P.Radix and chemical liver injury.Molecular docking was used to fit the components,which were subsequently verified in vitro.A cell model of hepatic fibrosis was established by activating hepatic stellate cell(HSC)-LX2 cells with 10 ng/mL transforming growth factor-β1.The cells were exposed to different concentrations of total glucosides of paeony(TGP),the active substance of P.Radix,and then evaluated using the cell counting kit-8 assay,enzyme-linked immunosorbent assay,and western blot.Results:Analysis through network pharmacology revealed 13 key compounds of P.Radix,and the potential targets for preventing chemical liver injury were IL-6,AKT serine/threonine kinase 1,jun protooncogene,heat shock protein 90 alpha family class A member 1(HSP90AA1),peroxisome proliferator activated receptor gamma(PPARG),PTGS2,and CASP3.Gene Ontology(GO)enrichment analysis indicated the involvement of response to drugs,membrane rafts,and peptide binding.Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis revealed that the main pathways involved lipid and atherosclerosis and chemical carcinogenesis-receptor activation.Paeoniflorin and albiflorin exhibited strong affinity for HSP90AA1,PTGS2,PPARG,and CASP3.Different concentrations of TGP can inhibit the expression of COL-I,COL-III,IL-6,TNF-a,IL-1β,HSP-90a,and PTGS2 while increasing the expression of PPAR-γand CASP3 in activated HSC-LX2 cells.Conclusion:P.Radix primarily can regulate targets such as HSP90AA1,PTGS2,PPARG,CASP3.TGP,the main active compound of P.Radix,protects against chemical liver injury by reducing the inflammatory response,activating apoptotic proteins,and promoting the apoptosis of activated HSCs.
文摘目的 白芍中药产品种类繁多,为提高白芍的质量控制水平,建立同时测定白芍药材及制剂中9个成分的一测多评(quantitative analysis of multi-components with a single-marker, QAMS)方法。方法 采用高效液相色谱(HPLC)法,以芍药苷为参照物,计算其他8种成分没食子酸、羟基芍药苷、儿茶素、芍药内酯苷、没食子酰芍药苷、五没食子酰葡萄糖、苯甲酸和苯甲酰芍药苷的相对校正因子(fk/s),并对fk/s进行耐用性考察。收集18批不同产地来源的白芍药材及其炮制品以及不同批号的白芍总苷胶囊,分别采用外标法和QAMS法测定9个成分含量,比较两者的差异,验证QAMS法的可行性和准确性。结果 各个成分的fk/s重复性良好,对18批样品运用QAMS法和外标法检测得到的各成分含量无显著性差异(RSD<4%);不同产地来源及炮制品的白芍药材中9个成分含量均存在较大差异,说明应提高白芍中药的质量控制标准;不同批次间白芍总苷胶囊的主要成分含量差异不大,说明制剂工艺相对稳定。结论 研究所建立的QAMS法准确性高,可用于白芍药材及其相关制剂的多成分同时定量分析,为白芍中药的全面质量控制提供方法参考。
