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Observation of Insulin Exocytosis by a Pancreatic β Cell Line with Total Internal Reflection Fluorescence Microscopy 被引量:7
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作者 Zhao-ying Fu Ya-ping Wang Yu Chen 《Chinese Medical Sciences Journal》 CAS CSCD 2011年第1期60-63,共4页
INSULIN secretion was traditionally measured with biochemical and immunological methods such as enzyme linked immunosorbant assay and radioimmunoassay. However, these methods can only tell the amount of insulin secret... INSULIN secretion was traditionally measured with biochemical and immunological methods such as enzyme linked immunosorbant assay and radioimmunoassay. However, these methods can only tell the amount of insulin secreted; they give no information about the secretion process or mechanism of exocytosis. In recent years, an imaging technique known as total internal reflection fluorescence (TIRF) microscopy has been employed to study insulin secretion. 展开更多
关键词 total internal reflection fluorescence microscopy EXOCYTOSIS INSULIN KISS-AND-RUN
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A Spectrometric Setup for Synchronous Total Internal Reflection Fluorescence Measurement at the Solid/Liquid Interface 被引量:1
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作者 Yao Qun LI Jia Ju XU +2 位作者 Run Tang WANG Li Jun YU Zhao LI 《Chinese Chemical Letters》 SCIE CAS CSCD 2002年第6期571-572,共2页
A spectrometric setup to perform total internal reflection fluorescence (TIRF) and synchronous TIRF measurements at solid/liquid interfaces is presented. The combination of TIRF and synchronous fluorescence was propo... A spectrometric setup to perform total internal reflection fluorescence (TIRF) and synchronous TIRF measurements at solid/liquid interfaces is presented. The combination of TIRF and synchronous fluorescence was proposed to analyze simultaneously different components at interfaces. The TIRF excitation, emission and synchronous spectra of a watersoluble porphyrin were obtained from water/glass interface using this setup without the existence of a surfactant. 展开更多
关键词 total internal reflection fluorescence synchronous fluorescence spectrofluorimeter meso-tetra(4-sulfonatophenyl)porphyrin.
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Optical anisotropy of black phosphorus by total internal reflection 被引量:1
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作者 Wei Xin Hao Bo Jiang +7 位作者 Teng Qian Sun Xiao Guang Gao Shao Nan Chen Bo Zhao Jian Jun Yang Zhi Bo Liu Jian Guo Tian Chun Lei Guo 《Nano Materials Science》 CAS 2019年第4期304-309,共6页
Although abundant research on the anisotropy of van der Waals(vd W)materials has been published,we undertake an in-depth study of their optical properties as they have an important guiding role for light control in tw... Although abundant research on the anisotropy of van der Waals(vd W)materials has been published,we undertake an in-depth study of their optical properties as they have an important guiding role for light control in two-dimensional(2D)nanospace.As an example,we study the reflectance of few-layered black phosphorus(BP)in the total internal reflection(TIR)mode in detail.We demonstrate that its optical anisotropy can be changed on a large scale by varying the incident angles,polarization states,and the in-plane rotation angles of the BP samples.Theoretical analysis indicates that the phenomena observed are common to all the atom-thick biaxial crystals,so these conclusions can be widely applied to other anisotropic 2D materials.This research furthers the current understanding of the properties of BP more comprehensively,and provides guidance for developing new optoelectronic applications,especially when BP and other atom-thick biaxial crystals are integrated with TIR devices. 展开更多
关键词 Black phosphorus Optical anisotropy total internal reflection Biaxial crystal
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Orientation Angle of Molecules at Hexadecane-Water Interface Studied with Total Internal Reflection Second Harmonic Generation
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作者 Li-bo Zhang Hui Fang +2 位作者 Shun-li Chen Xue-feng Zhu Wei Gan 《Chinese Journal of Chemical Physics》 SCIE CAS CSCD 2016年第6期650-656,I0001,共8页
The orientation angle is an important parameter that reflects the structure of molecules at interfaces. In order to obtain this parameter, second order nonlinear spectroscopic techniques including second harmonic gene... The orientation angle is an important parameter that reflects the structure of molecules at interfaces. In order to obtain this parameter, second order nonlinear spectroscopic techniques including second harmonic generation (SHG) and sum frequency generation-vibrational spec- troscopy (SFG-VS) have been successfully applied through analysis of the nonlinear signal from various polarizations. In some SHG and SFG-VS experiments, total internal reflection (TIR) configuration has been adopted to get enhanced signals. However, the reports on the detailed procedure of the polarization analysis and the calculation of the orientation angle of interracial molecules under TIR configuration are still very few. In this paper, we mea- sured the orientation angles of two molecules at the hexadecane-water interface under TIR and Non-TIR experimental configurations. The results measured from polarization analysis in TIR configuration consist with those obtained from Non-TIR configuration. This work demonstrates the feasibility and accuracy of polarization analysis in the determination of the orientation angle of molecules at the interfaces under TIR-SHG configuration. 展开更多
关键词 Second harmonic generation total internal reflection HYPERPOLARIZABILITY Polarization Orientation angle
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Adsorption Behavior of a Water-soluble Porphyrin at the Glass-water Interface as Studied by Synchronous Total Internal Reflection Fluorescence Spectroscopy
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作者 MinNaYAO YaoQunLI 《Chinese Chemical Letters》 SCIE CAS CSCD 2004年第1期109-111,共3页
Total internal reflection fluorescence spectroscopy (TIRF) and synchronous scanning technique were combined to study the adsorption behavior of the meso-tetrakis (4-sulfonatophenyl) porphyrin (TPPS) at the glass-water... Total internal reflection fluorescence spectroscopy (TIRF) and synchronous scanning technique were combined to study the adsorption behavior of the meso-tetrakis (4-sulfonatophenyl) porphyrin (TPPS) at the glass-water interface without any surfactant. The pH dependence of synchronous fluorescence signal at the interface was analyzed. Both unprotonated (TPPS4-) and diprotonated (H2TPPS2-) forms of TPPS were observed at the interface. But the interface favored the adsorption of. The apparent estimated pKa2 value shifted from 5.00 in the bulk solution to 2.7 at the interface. STIRF provides a good technique to study multi-component systems at the interface. 展开更多
关键词 total internal reflection fluorescence synchronous fluorescence water- soluble porphyrin pH dependence glass-water interface.
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Determination of Proteins by Measuring Total Internal-reflected Resonance Light Scattering Signals on Water/Tetrachloromethane Interface with Evans Blue and Cetyltrimethylammonium Bromide
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作者 HUANGCheng-zhi FENGPing LIYuan-fang ZHAOHua-wen 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2005年第4期426-430,共5页
A sensitive and selective assay of proteins is proposed based on measuring the total internal-reflected resonance light scattering(TIR-RLS) signals produced on the water/tetrachloromethane(H_2O/CCl_4) interface. In an... A sensitive and selective assay of proteins is proposed based on measuring the total internal-reflected resonance light scattering(TIR-RLS) signals produced on the water/tetrachloromethane(H_2O/CCl_4) interface. In an aqueous medium with pH value in the range of 3.29—3.78, electrostatic attraction occurs between the negatively charged Evans Blue(EB) and positively charged proteins, forming hydrophobic ion associates and resulting in EB-protein adsorption on H_2O/CCl_4 interface. The presence of cetyltrimethylammonium bromide prompts this adsorption, resulting in strongly enhanced TIR-RLS signals. The intensity of the enhanced TIR-RLS at 360—370 nm was found to be proportional to the concentration of proteins. For bovine serum albumin and human serum albumin, the linear range of detection is 0.07—1.2 μg/mL and the limits of detection are 6.68 and 6.30 ng/mL(3σ), respectively, while for lysozyme, the linear range of detection is 0.06—1.0 μg/mL and the limit of detection is 6.0 ng/mL(3σ). The content of the total albumin in a human urine sample could be directly determined by using the standard addition method with a percent recovery of 97.6%—104.1%, and the RSD ranging from 1.9% to 4.2%. 展开更多
关键词 total internal-reflected resonance light scattering(tir-RLS) Protein Evans Blue(EB) Cetyltrimethylammonium bromide(CTMAB)
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Total Internal Reflection(TIR)Behavior of Heterogeneous Interface Shear Waves in Layered Soft Structure
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作者 Ming Zhao Wentao Jiang +3 位作者 Qingyuan Wang Ying Liang Haidong Fan Xiaobao Tian 《Acta Mechanica Solida Sinica》 SCIE EI CSCD 2024年第5期662-672,共11页
The total internal reflection(TIR)behavior of interface shear waves is crucial for ensuring the reliability of dielectric elastomer(DE)devices.However,due to the complex force-electric coupling and large deformation o... The total internal reflection(TIR)behavior of interface shear waves is crucial for ensuring the reliability of dielectric elastomer(DE)devices.However,due to the complex force-electric coupling and large deformation of DEs,the TIR behavior of shear waves in heterogeneous force-electric interface models is still unclear.This study modeled an elastic/DE bi-material interface to analyze the trajectory of out-of-plane shear waves.Employing Dorfmann and Ogden’s nonlinear electroelastic framework and the related linear small incremental motion theory,a method has been developed to control the TIR behavior of interface shear waves.It has been found that the TIR behavior is significantly influenced by the strain-stiffening effect induced by biasing fields.Consequently,a biasing field principle involving preset electric displacement and pre-stretch has been proposed for TIR occurrence.By controlling the pre-stretch and preset electric displacement,active regulation of TIR behavior can be achieved.These results suggest a potential method for achieving autonomous energy shielding to improve the reliability of DE devices. 展开更多
关键词 total internal reflection(tir) Heterogeneous interface model Dielectric elastomers(DEs) Strain-stiffening
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Practical applications of total internal reflection fluorescence microscopy for nanocatalysis
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作者 Chengyang Yan Xuanhao Mei +1 位作者 Xue Gong Weilin Xu 《Industrial Chemistry & Materials》 2024年第1期85-99,共15页
Fluorescence microscopy has evolved from a purely biological tool to a powerful chemical instrument for imaging and kinetics research into nanocatalysis.And the demand for high signal-to-noise ratio and temporal–spat... Fluorescence microscopy has evolved from a purely biological tool to a powerful chemical instrument for imaging and kinetics research into nanocatalysis.And the demand for high signal-to-noise ratio and temporal–spatial resolution detection has encouraged rapid growth in total internal reflection fluorescence microscopy(TIRFM).By producing an evanescent wave on the glass–water interface,excitation can be limited to a thin plane to ensure the measured accuracy of kinetics and image contrast of TIRFM.Thus,this unique physical principle of TIRFM makes it suitable for chemical research.This review outlines applications of TIRFM in the field of chemistry,including imaging and kinetics research.Hence,this review could provide guidance for beginners employing TIRFM to solve current challenges creatively in chemistry. 展开更多
关键词 total internal reflection fluorescence microscopy NANOCATALYSIS IMAGING Kinetics analysis
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Observation of tiny polarization rotation rate in total internal reflection via weak measurements 被引量:2
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作者 CHENGQUAN MI SHIZHEN CHEN +3 位作者 XINXING ZHOU KAI TIAN HAILU LUO SHUANGCHUN WEN 《Photonics Research》 SCIE EI 2017年第2期92-96,共5页
In this paper,we examine the tiny polarization rotation effect in total internal reflection due to the spin–orbit interaction of light.We find that the tiny polarization rotation rate will induce a geometric phase gr... In this paper,we examine the tiny polarization rotation effect in total internal reflection due to the spin–orbit interaction of light.We find that the tiny polarization rotation rate will induce a geometric phase gradient,which can be regarded as the physical origin of photonic spin Hall effect.We demonstrate that the spin-dependent splitting in position space is related to the polarization rotation in momentum space,while the spin-dependent splitting in momentum space is attributed to the polarization rotation in position space.Furthermore,we introduce a quantum weak measurement to determine the tiny polarization rotation rate.The rotation rate in momentum space is obtained with 118 nm,which manifests itself as a spatial shift,and the rotation rate in position space is achieved with 38 μrad∕λ,which manifests itself as an angular shift.The investigation of the polarization rotation characteristics will provide insights into the photonic spin Hall effect and will enable us to better understand the spin–orbit interaction of light. 展开更多
关键词 rate Observation of tiny polarization rotation rate in total internal reflection via weak measurements
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Research of Asymmetric Y-Branching Total Internal Reflection All-Optical Switch
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作者 Kejian Chen, Hengzheng Zhan, Yi Tang, Ailing Yang, Xihua Li, Jianyi Yang, Xiaoqing Jiang, Minghua Wang Department of Information Science and Electronic Engineering, Zhejiang University, Hangzhou 310027,P.R.China 《光学学报》 EI CAS CSCD 北大核心 2003年第S1期137-138,共2页
Y-branching TIR all-optical switch have been fabricated. When the switching optical intensity is 149.9W/mm2, the extinction ratio is 18dB. A theoretical model was also proposed which provided a good fit to the experim... Y-branching TIR all-optical switch have been fabricated. When the switching optical intensity is 149.9W/mm2, the extinction ratio is 18dB. A theoretical model was also proposed which provided a good fit to the experimental data. 展开更多
关键词 of on In in IS as Research of Asymmetric Y-Branching total internal reflection All-Optical Switch GAAS
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CD146 detection with real-time total internal reflection imaging ellipsometry
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作者 Li Liu1,3 ,Yu Niu 1,3,YongHong Meng1,She Chen1,XiYun Yan2 , Gang Jin1 1Institute of Mechanics, Chinese Academy of Sciences, Beijing 100190, China 2Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China 3Graduate School, Chinese Academy of Sciences, Beijing 100049, China 《生物物理学报》 CAS CSCD 北大核心 2009年第S1期294-295,共2页
Adhesion molecule CD146 (100-130kDa) belongs to the immunoglobulin super family and it is originally identified as a biomarker for melanoma. Recently, CD146 is found as
关键词 CD146 detection with real-time total internal reflection imaging ellipsometry TIME REAL
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傅里叶变换衰减全反射红外光谱法(ATR-FTIR)的原理与应用进展 被引量:111
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作者 黄红英 尹齐和 《中山大学研究生学刊(自然科学与医学版)》 2011年第1期20-31,共12页
傅里叶变换衰减全反射红外光谱(ATR-FTIR)在难以制备的样品、无损检测及表面信息的获取等方面具有独特的优势。本文对傅里叶变换衰减全反射红外光谱(ATR-FTIR)的基本原理、特点及应用进展进行了较为全面的综述,对从事此方面相关工作的... 傅里叶变换衰减全反射红外光谱(ATR-FTIR)在难以制备的样品、无损检测及表面信息的获取等方面具有独特的优势。本文对傅里叶变换衰减全反射红外光谱(ATR-FTIR)的基本原理、特点及应用进展进行了较为全面的综述,对从事此方面相关工作的研究具有一定的参考价值。 展开更多
关键词 衰减全反射 傅里叶变换红外光谱 原理 应用 无损分析 表面分析
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C_(6v)对称TIR-PCF结构参数对基模零色散点的调节数值研究 被引量:2
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作者 汤炳书 《计算物理》 CSCD 北大核心 2008年第1期101-105,共5页
用多极方法数值研究构成六重对称全内反射型光子晶体光纤各种结构参数对基模零色散点的调节.数值结果表明,在相对孔径较大时,包层空气孔柱层数不影响零色散点,在其它结构参数相同的条件下,空气孔直径越大,零色散点波长越短,而在其它结... 用多极方法数值研究构成六重对称全内反射型光子晶体光纤各种结构参数对基模零色散点的调节.数值结果表明,在相对孔径较大时,包层空气孔柱层数不影响零色散点,在其它结构参数相同的条件下,空气孔直径越大,零色散点波长越短,而在其它结构参数相同的条件下,空气孔孔距越大,零色散点波长越长,当空气孔直径与空气孔孔距都很小时,零色散点出现在长波区域,当空气孔直径与空气孔孔距大到一定程度时,色散曲线在零色散线出现振荡特性,并出现多个零色散点,在包层空气柱层数一定的条件下,中央纤芯缺省空气孔层数对零色散点的调节范围大. 展开更多
关键词 六重对称全内反射型光子晶体光纤 多极方法 零色散点 结构参数
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FTIR装置调Q开关性能参数及其测试的研究
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作者 刘晖 朱煜 +1 位作者 朱日宏 陈进榜 《光子学报》 EI CAS CSCD 1998年第11期1038-1040,共3页
本文分析了受抑全内反射(Frustratedtotalinternalreflection)的理论基础,研究光线透射率、反射率相对于棱镜相对间隙d/λ之间的关系,建立了一套实验装置对其进行验证.
关键词 受抑全内反射 相对间隙 Ftir 调Q开关 性能
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TIRF研究罗丹明-640在石英表面的吸附(英文) 
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作者 于安池 李密 赵新生 《物理化学学报》 SCIE CAS CSCD 北大核心 1998年第8期682-685,共4页
利用全反射激发荧光(TIRF)技术对罗丹明-640分子在石英表面上的吸附进行了研究.实验表明TIRF可以比较全面提供表面吸附的热力学和动力学信息.罗丹明-640分子在石英表面上的吸附自由能约为-32kJ·mol-1.
关键词 吸附 荧光 tirF 石英表面 罗丹明640 固液界面
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C_(6v)对称六角与圆形空气柱TIR-PCF基模色散比较 被引量:1
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作者 汤炳书 《光通信研究》 北大核心 2016年第3期40-42,共3页
设计了一种由六角形空气柱包层组成的C_(6v)(六重旋转对称)全内反射型PCF(光子晶体光纤),采用全矢量有限元法研究其基模色散,数值研究结果表明,在其他参数不变的情况下,包层空气柱由圆形变成六角形后两基模偏振态会出现分离现象,存在较... 设计了一种由六角形空气柱包层组成的C_(6v)(六重旋转对称)全内反射型PCF(光子晶体光纤),采用全矢量有限元法研究其基模色散,数值研究结果表明,在其他参数不变的情况下,包层空气柱由圆形变成六角形后两基模偏振态会出现分离现象,存在较大的模式双折射与较高的PMD(偏振模色散),基模的波长色散在X偏振时与包层圆形空气柱时有相同的变化曲线,说明包层空气柱形状对基模色散特性有较大影响。 展开更多
关键词 六重旋转对称全内反射型光子晶体光纤 全矢量有限元法 基模色散
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基于FTIR的多点触摸实时互动桌面系统 被引量:5
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作者 赵秋均 王毅刚 +1 位作者 黄岸仲 吴钰辉 《计算机工程》 CAS CSCD 北大核心 2011年第14期291-292,F0003,共3页
针对2010年上海世博会主题馆设计需求,提出并实现一种低成本的多点触摸实时互动桌面系统。以受抑全内反射(FTIR)技术为核心,介绍多点触摸屏的设计和实现。基于计算机视觉,采用改进的最小距离优先算法和字符串相似度算法,实现对交互手势... 针对2010年上海世博会主题馆设计需求,提出并实现一种低成本的多点触摸实时互动桌面系统。以受抑全内反射(FTIR)技术为核心,介绍多点触摸屏的设计和实现。基于计算机视觉,采用改进的最小距离优先算法和字符串相似度算法,实现对交互手势的识别。通过基于软件的几何校准和边缘融合,对多投影机画面进行无缝拼接,形成一个逻辑上统一的大屏幕。应用结果表明,该系统具有较好的实时性、交互性和娱乐性。 展开更多
关键词 受抑全内反射 多点触摸 手势识别 互动桌面 屏幕拼接
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FTIR结构中光子隧穿的位相时间和横向位移
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作者 闫爱民 李春芳 《量子光学学报》 CSCD 2001年第2期63-66,共4页
计算了TE光子穿越受阻全内反射 (Frustratedtotalinternalreflection———FTIR)结构的隧穿时间 (位相时间 )以及光子透射过势垒后产生的横向位移。利用金属膜理论中阐述的复数折射角的定义得到了这种典型势垒结构中单层均匀媒质的特性... 计算了TE光子穿越受阻全内反射 (Frustratedtotalinternalreflection———FTIR)结构的隧穿时间 (位相时间 )以及光子透射过势垒后产生的横向位移。利用金属膜理论中阐述的复数折射角的定义得到了这种典型势垒结构中单层均匀媒质的特性矩阵M ,通过计算入射波电场和透射波电场的复数振幅比 ,得到了光子隧穿势垒的复透射系数及产生的横向位移。最后 ,对所得的计算结果进行了分析和讨论。 展开更多
关键词 位相时间 横向位移 受阻全内反射 Ftir结构 光子 隧道效应 透射矩阵法 势垒
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单次反射ATR-FTIR红外光谱法在涂膜表面成分分析中的应用 被引量:1
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作者 赵蓉旭 《中国涂料》 CAS 2015年第1期67-71,共5页
运用傅里叶变换衰减全反射红外光谱法(ATR-FTIR)中的单次反射法对水性偏氯乙烯-丙烯酸酯共聚物薄膜进行辅助定性研究,考察了具有不同偏氯乙烯单体含量的共聚物薄膜的红外谱图变化。同时运用单次反射ATR-FTIR法对施工现场取回的红色脱落... 运用傅里叶变换衰减全反射红外光谱法(ATR-FTIR)中的单次反射法对水性偏氯乙烯-丙烯酸酯共聚物薄膜进行辅助定性研究,考察了具有不同偏氯乙烯单体含量的共聚物薄膜的红外谱图变化。同时运用单次反射ATR-FTIR法对施工现场取回的红色脱落涂膜进行定性分析。 展开更多
关键词 单次反射衰减全反射 ATR-Ftir 偏氯乙烯-丙烯酸酯共聚物
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Three-dimensional tracking of GLUT4 vesicles in TIRF microscopy
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作者 Xiang-ping WU Jie-yue LI +2 位作者 Ying-ke XU Ke-di XU Xiao-xiang ZHENG 《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 SCIE EI CAS CSCD 2008年第2期232-240,共9页
TIRF microscopy has provided a means to view mobile granules within 100 nm in size in two dimensions.However quantitative analysis of the position and motion of those granules requires an appropriate tracking method.I... TIRF microscopy has provided a means to view mobile granules within 100 nm in size in two dimensions.However quantitative analysis of the position and motion of those granules requires an appropriate tracking method.In this paper,we present a new tracking algorithm combined with the unique features of TIRF.Firstly a fluorescence correction procedure was processed to solve the problem of fluorescence bleaching over time.Mobile granules were then segmented from a time-lapse image stack by an adaptive background subtraction method.Kalman filter was introduced to estimate and track the granules that allowed reducing searching range and hence greater reliability in tracking process.After the tracked granules were located in x-y plane,the z-position was indirectly inferred from the changes in their intensities.In the experiments the algorithm was applied in tracking GLUT4 vesicles in living adipose cells.The results indicate that the algorithm has achieved robust estimation and tracking of the vesicles in three dimensions. 展开更多
关键词 GLUT4 total internal reflection fluorescence (tirF) microscopy Adaptive background subtraction Kalman filter Fluorescence correction
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