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Elementary study of differential expression of total soluble proteins in different life phases of Porphyra yezoensis
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作者 孟春晓 高政权 +2 位作者 王依涛 罗韬 叶乃好 《Marine Science Bulletin》 CAS 2010年第2期32-37,共6页
Total soluble proteins of different life stages, filamentous sporophytes cultivated in high temperatures, and blade gametophytes harvested in different seasons, were identified by SDS-PAGE. The types and amounts of ex... Total soluble proteins of different life stages, filamentous sporophytes cultivated in high temperatures, and blade gametophytes harvested in different seasons, were identified by SDS-PAGE. The types and amounts of expressed proteins also varied amongst the samples. The fewest soluble proteins were present in filamentous sporophytes. There were more types and amounts of soluble protein in conchospores than in filamentous sporophytes, but fewer than in bulgy sporophytes. More types of protein were detected in filamentous sporophytes cultivated in high temperatures than in those growing in normal situations. The most types and amounts of protein were found in blade gametophytes in all samples. Blade gametophytes harvested last year and stored at -20 ℃ showed only minor differences in expression of proteins when compared with those harvested in different seasons. 展开更多
关键词 Porphyra yezoensis total soluble proteins differential expression
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Effect of Wheat Based Diet Supplemented with Xylanase on Blood Sugar and Total Protein in Serum of Geese 被引量:5
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作者 王佳丽 史东辉 杨桂芹 《Agricultural Science & Technology》 CAS 2008年第2期98-100,121,共4页
[Objective] The effects of wheat based diet supplemented with xylanase on blood sugar and total protein in serum of geese were studied. [ Method ] By using the randomized design of single factor, the 1-day-old healthy... [Objective] The effects of wheat based diet supplemented with xylanase on blood sugar and total protein in serum of geese were studied. [ Method ] By using the randomized design of single factor, the 1-day-old healthy goslings were divided into 6 groups and fed with corn based diet, wheat based diet and wheat based diet supplemented with xylanase at different concentrations respectively, the contents of blood sugar and total protein in serum were determined. [ Result] The wheat based diet supplemented with xylanase could increase the blood sugar and total protein in serum of geese and wheat based diet supplemented with 0.2% xylanase generated the best effect, which was higher than those of corn based diet group. As for the concentration of protein in serum, wheat based diet supplemented with 0.2% xylanase was significantly different from corn based diet and wheat based diet. [ Conclusion] The wheat based diet supplemented with xylanase could enhance geese production. 展开更多
关键词 XYLANASE GEESE Blood sugar total protein in serum
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Effects of Cold Acclimation and CaCl_2 on Total Soluble Protein, CaM and Freezing Resistance of Populus tomentosa Seedlings 被引量:6
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作者 林善枝 张志毅 《Forestry Studies in China》 CAS 2002年第1期5-12,共8页
Populus tomentosa seedlings used for cold acclimation at -3℃ were pretreated with or without 10?mmol·L -1 CaCl 2, 3?mmol·L -1 of Ca 2+ chelator EGTA, 0 1?mmol·L -1 of Ca 2+ ... Populus tomentosa seedlings used for cold acclimation at -3℃ were pretreated with or without 10?mmol·L -1 CaCl 2, 3?mmol·L -1 of Ca 2+ chelator EGTA, 0 1?mmol·L -1 of Ca 2+ channel inhibitor LaCl 3,and 0 05?mmol·L -1 of CaM antagonist CPZ. The changes in the contents of total soluble protein and CaM, and freezing resistance in all pretreated seedlings in various periods ( viz: following cold acclimation, chilling stress and recovery) were investigated. Results showed that cold acclimation increased the contents of total soluble protein and CaM, and freezing resistance of seedlings, which could be strongly reduced by the pretreatments of EGTA CPZ and LaCl 3 Cold acclimation combined with CaCl 2 pretreatment enhanced the effect of cold acclimation on freezing resistance, and obviously increased the contents of total soluble protein and CaM, reduced the declining degree of the contents of total soluble protein and CaM caused by chilling stress as compared with cold acclimation, augmented the increase in the level of total soluble protein and CaM during the recovery periods. Further analysis found that an increase in total soluble protein content during cold acclimation with or without CaCl 2 pretreatment mainly resulted from the increase in content of heat stable protein in total soluble protein. It is suggested that Ca 2+ calmodulin may be involved in the synthesis of total soluble protein, and the induction of freezing resistance of seedlings. 展开更多
关键词 Populus tomentosa cold acclimation CaCl 2 total soluble protein CAM freezing resistance
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Cold Acclimation-Induced Changes in Total Soluble Protein, RNA, DNA, RNase and Freezing Resistance in Populus tomentosa Cuttings 被引量:3
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作者 林善枝 张志毅 《Forestry Studies in China》 CAS 2002年第2期9-15,共7页
The changes in the contents of total soluble protein and RNA, the activity of RNase in leaves and branches of Populus tomentosa cuttings at various periods (viz: cold acclimation, deacclimation, chilling stress an... The changes in the contents of total soluble protein and RNA, the activity of RNase in leaves and branches of Populus tomentosa cuttings at various periods (viz: cold acclimation, deacclimation, chilling stress and the recovery after chilling stress), and the survival rate and the freezing resistance of cuttings during cold acclimation at -3℃ were investigated. Results showed that cold acclimation not only increased the contents of total soluble protein and RNA, the survival rates and the freezing resistance of cuttings, decreased the activity of RNase, but also reduced the declining degree of total soluble protein and RNA contents, and the increasing level of RNase caused by chilling stress as compared with the controls. In addition, cold acclimation augmented the increase in the level of total soluble protein and RNA, and facilitated the decrease of RNase during the recovery periods. Further analysis found that the DNA content of all treatments kept relative stability at various periods. The changes in total soluble protein, RNA and RNase were closely related to the freezing resistance of cuttings. It appears that the increase of RNA content caused by cold acclimation induced decrease of RNase activity may be involved in the accumulation of total soluble protein and the induction of freezing resistance of cuttings. 展开更多
关键词 Populus tomentosa cold acclimation freezing resistance total soluble protein nucleic acid
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Effect of Light Emitting Diodes (LEDs) on Growth, Mineral Composition, and Nutritional Value of Wheat & Lentil Sprouts
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作者 Abdul Momin Amana Khatoon +10 位作者 Wajahat Khan Dilsat Bozdoğan Konuşkan Muhammad Mudasar Aslam Muhammad Jamil Shafiq Ur Rehman Baber Ali Alevcan Kaplan Sana Wahab Muhammad Nauman Khan Sezai Ercisli Mohammad Khalid Al-Sadoon 《Phyton-International Journal of Experimental Botany》 SCIE 2024年第6期1117-1128,共12页
Sprouts are ready-to-eat and are recognized worldwide as functional components of the human diet.Recent advances in innovative agricultural techniques could enable an increase in the production of healthy food.The use... Sprouts are ready-to-eat and are recognized worldwide as functional components of the human diet.Recent advances in innovative agricultural techniques could enable an increase in the production of healthy food.The use of light-emitting diode(LED)in indoor agricultural production could alter the biological feedback loop,increasing the functional benefits of plant foods such as wheat and lentil sprouts and promoting the bioavailability of nutrients.The effects of white(W),red(R),and blue(B)light were investigated on the growth parameters and nutritional value of wheat and lentil sprouts.In the laboratory,seeds were sown under three different LED treat-ments:white,red,and blue light,while normal incandescent light served as a control.Percentage seed germina-tion improved by 18.34%and 12.67%for wheat and 18.34%and 12.67%for lentil sprouts under LED treatments R and B,respectively.An increase in total soluble protein and sugar by 33.4%and 9.23%in wheat and by 31.5%and 5.87%in lentils was observed under the R LED treatment.Vitamin C concentrations in wheat and lentils were significantly increased by R LED compared to all other treatments.Other parameters,including potassium and sodium concentrations,were significantly increased under red and blue light compared to the control;white light,on the other hand,significantly decreased all these parameters.According to the experimental data,red and blue LED light could be beneficial in the production of functional wheat and lentil sprouts with high nutrient concentrations. 展开更多
关键词 Growth parameters LED nutritional value SPROUTS total soluble proteins
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Effects of Nitrogen Fertigation Rate on Protein Components in Grains and Processing Quality of Different Wheat Varieties 被引量:5
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作者 池忠志 赵广才 +2 位作者 郑家国 杨玉双 姜心禄 《Agricultural Science & Technology》 CAS 2012年第2期370-374,共5页
[Objective] This study aimed to explore the effects of nitrogen fertigation rate on the protein components in grains and processing quality of different wheat varieties. [Method] Under the condition of higher soil fer... [Objective] This study aimed to explore the effects of nitrogen fertigation rate on the protein components in grains and processing quality of different wheat varieties. [Method] Under the condition of higher soil fertility, different amounts of N-fertilizer were applied in the plots, and then the contents of total protein and its components, percentage of the component content to total protein content as well as the processing quality of grains of two strong-gluten wheat varieties (Linyou145 and Zhengmai9023) and two weak-gluten wheat varieties (Ningmai9 and Baofeng949) were determined. [Result] The contents of total protein and globulin, gliadin and glutenin were improved significantly with the increase of the N-fertilizer amount; but the content of albumin did not show remarkable increase; in addition, the percentage of each protein component was relative stable and did not increase significantly. Increase in the amount of N-fertilizer improved the sedimentation value, wet gluten content, loaf volume and loaf score, decreased the volume weight of grain. [Conclusion] This study provideed theoretical support for high-quality wheat production. 展开更多
关键词 Nitrogen fertilizer amount total protein content Contents of protein components Percentage of protein components
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Identification of Expression of CpTI Gene in Transgenic Poplars at Protein Level
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作者 林元震 张谦 +1 位作者 林善枝 张志毅 《Forestry Studies in China》 CAS 2002年第2期33-37,共5页
The contents of total soluble protein and cowpea trypsin inhibitor (CpTI) in the browse and metaphylla of transgenic hybrid triploid poplars [ (Populus tomentosa×P.bolleana)×P.tomentosa ] transformed with ... The contents of total soluble protein and cowpea trypsin inhibitor (CpTI) in the browse and metaphylla of transgenic hybrid triploid poplars [ (Populus tomentosa×P.bolleana)×P.tomentosa ] transformed with CpTI gene were determined in order to study the products of CpTI gene expression at protein level. The results indicated that the amount of total soluble protein was greater in transgenic poplars than in non transgenic poplars, but was more in the metaphylla than in browse. The expression of CpTI gene resulted in an obvious increase in CpTI content, whereas CpTI was not detected in non transgenic poplars. It was found that there were high amount of total soluble protein and CpTI in 3 clones of TG07, TG04 and TG71 compared with other transgenic clones. In addition, the analysis of protein by SDS PAGE showed that a specific protein band of about 11.3?kD corresponding to the 80 amino acids encoded by the CpTI gene was observed in transgenic poplars on the gel of protein, which was not detected in non transgenic poplars. 展开更多
关键词 Populus tomentosa TRANSFORMATION CPTI CpTI gene total soluble protein
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17α,20β-DIHYDROXY-4-PREGNENE-3-ONE SUPPRESSES PLASMA IgM LEVELS IN RAINBOW TROUT (Oncorhynchus mykiss)
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作者 侯亚义 韩晓冬 铃木让 《Zoological Research》 CAS CSCD 2000年第2期97-102,共6页
The relationships between 17α,20β dihydroxy 4 pregnene 3 one (17α,20β DP) and plasma IgM and total protein levels were investigated in rainbow trout, Oncorhynchus mykiss .IgM and total protein levels decre... The relationships between 17α,20β dihydroxy 4 pregnene 3 one (17α,20β DP) and plasma IgM and total protein levels were investigated in rainbow trout, Oncorhynchus mykiss .IgM and total protein levels decreased in both sexes of mature rainbow trout when 17α,20β DP levels increased during the spawning season,while the elevated 17α,20β DP suppressed IgM levels and reversibly enhanced total protein concentrations in immature trout.This represents the first report of the effect of 17α,20β DP on fish immunity.These data suggest that the increase of 17α,20β DP in spawning season may be related to infectious skin diseases. 展开更多
关键词 Rainbow trout Immunoglobulin M 17α 20β-dihydroxy-4-pregnene-3-one Plasma total protein concentration
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Atmospheric pressure gliding arc discharge plasma treatments for improving germination, growth and yield of wheat 被引量:5
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作者 N C ROY M M HASAN +3 位作者 A H KABIR M A REZA M R TALUKDER A N CHOWDHURY 《Plasma Science and Technology》 SCIE EI CAS CSCD 2018年第11期90-100,共11页
Wheat (Triticum aestivum) seeds were treated with atmospheric pressure gliding arc discharge plasmas to investigate the effects on water absorption, seed germination rate, seedling growth and yield in wheat. The sur... Wheat (Triticum aestivum) seeds were treated with atmospheric pressure gliding arc discharge plasmas to investigate the effects on water absorption, seed germination rate, seedling growth and yield in wheat. The surface architectures and functionalities of the seeds were found to modify due to plasma treatments. 6 rain treatment was provided 95%-100% germination rate. For the treatment duration of 3 and 9 rain the growth activity, dry matter accumulation, leaves chlorophyll contents, longest spikes, number of spikes/spikelet and total soluble protein content in shoots were improved. The grain yield of wheat was increased ,--20% by 6 min treatment with H2O/O2 plasma with respect to control. 展开更多
关键词 atmospheric pressure gliding arc discharge plasmas WHEAT GERMINATION growth improvement total soluble protein
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Rice plant growth and yield: foliar application of plasma activated water 被引量:1
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作者 M M RASHID Mamunur RASHID +1 位作者 M M HASAN M R TALUKDER 《Plasma Science and Technology》 SCIE EI CAS CSCD 2021年第7期134-143,共10页
Plasma activated water(PAW)was prepared for 10 min to be applied one tofive times as a foliar spray to rice plants,to investigate plant growth,yield and the concentrations of total soluble protein and sugar in the rice... Plasma activated water(PAW)was prepared for 10 min to be applied one tofive times as a foliar spray to rice plants,to investigate plant growth,yield and the concentrations of total soluble protein and sugar in the rice grains produced.The results reveal that(1)the plant height,stem diameter,dry weight,chlorophyll and total carotene concentrations were improved by∼15%,∼25%,∼24%,∼47%and∼45%,respectively,with respect to control,(2)defense mechanisms of the plants treated with PAW were improved,(3)concentrations of total soluble protein and sugar were enhanced in the rice grains of PAW treated plants and(4)yield was increased by∼14%. 展开更多
关键词 plasma agriculture plasma activated water rice plant growth rice yield total soluble protein
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THE PREPARATION OF CROSSLINKED N,O-CARBOXYMETHYL CHITOSAN RESINSAND THEIR ADSORPTION PROPERTIESFOR TRIGLYCERIDE IN SERUM 被引量:2
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作者 Yu Yihau He Binglin Gu Hanqing 《Chinese Journal of Reactive Polymers》 1997年第1期83-86,共4页
Crosslinked N, O-carboxymethyl chitosan resins which can selectively adsorb triglyceride, were prepared by the reaction of N, O- carboxymethyl chitosan with glutaraldehyde solution. Adsorption experiments were perform... Crosslinked N, O-carboxymethyl chitosan resins which can selectively adsorb triglyceride, were prepared by the reaction of N, O- carboxymethyl chitosan with glutaraldehyde solution. Adsorption experiments were performed by adding the resins to the serum. The results showed that this type of adsorbent could cut down the concentration of triglyceride in serum by 56. 4% (3. 35mg/gresin ) at most, while concentration of the total protein (TP ) decreased only by 10. 9% at least, so this novel adsorbent can be used to cure hypertriglyceridemia by hemoperfusion in the future. 展开更多
关键词 Crosslinked N O-carboxymethyl chitosan TRIGLYCERIDE Serum total protein (TP ) HYPERTRIGLYCERIDEMIA Adsorption
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A algorithm for prediction of exudative retinal detachment risk of patients with pregnancy-induced hypertension
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作者 Li Hu Dong-Hao Li Shuang-Yong Wang 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2022年第8期1310-1315,共6页
AIM:To investigate the risk of exudative retinal detachment(ERD)morbidity in patients with pregnancyinduced hypertension(PIH)by using the logistic regression combined with the receiver operating characteristic(ROC)cur... AIM:To investigate the risk of exudative retinal detachment(ERD)morbidity in patients with pregnancyinduced hypertension(PIH)by using the logistic regression combined with the receiver operating characteristic(ROC)curve.METHODS:A total of 46 patients with ERD and 142 patients with non-ERD were diagnosed as PIH from January 2017 to February 2020.A retrospective comparison of the clinical manifestations and laboratory tests were conducted.The risk of ERD morbidity with PIH was predicted by using logistic regression combined with an ROC curve model.RESULTS:There was no significant difference in age and body mass index between the two groups before pregnancy(P>0.05).However,significant differences were found in gestational weeks,duration of hypertension,maximum and minimum systolic and diastolic blood pressure(BP),and plasma total protein(PTP)concentration between the two groups(P<0.05).Binary logistic regression analysis showed that the maximum systolic BP(OR=1.050,95%CI:1.016-1.085)and PTP concentration(OR=0.764,95%CI:0.702-0.832)were independent prediction risks of ERD in PIH.The sensitivities of maximum systolic BP,PTP concentration and combined diagnosis were 0.717,0.870,and 0.870,respectively;the specificities were 0.617,0.837,and 0.908,respectively;the area under the curve(AUC)was 0.707(95%CI:0.622-0.792),0.917(95%CI:0.868-0.967),and 0.933(95%CI:0.890-0.975),respectively;the AUC of combined diagnosis was higher than that of single diagnosis(P<0.01).CONCLUSION:Logistic regression and ROC curve model combined with maximum systolic BP and PTP can improve the early identification of high-risk PIH patients in the hospital. 展开更多
关键词 pregnancy-induced hypertension exudative retinal detachment plasma total protein blood pressure
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Tau protein,phosphorylated tau protein,and beta-amyloid 42 levels in patients with neurodegenerative diseases complicated by cognitive deficits A non-randomized,concurrent,case-control investigation
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作者 Radomír Talb Jií Masopust +3 位作者 Ctirad Andrys Pavel touraè Jakub Hort Martin Vali 《Neural Regeneration Research》 SCIE CAS CSCD 2009年第11期951-957,共7页
BACKGROUND: The differential diagnosis of many neurodegenerative disorders depends primarily on clinical symptoms together with imaging methods. Recently, increased importance has been placed on the use of biomarkers... BACKGROUND: The differential diagnosis of many neurodegenerative disorders depends primarily on clinical symptoms together with imaging methods. Recently, increased importance has been placed on the use of biomarkers for diagnosing various neurodegenerative disorders. OBJECTIVE: To assess the feasibility of tau-protein, phosphorylated tau-protein, beta-amyloid 42 (Aβ42), and 14-3-3 protein as biomarkers for diagnosing several neurodegenerative diseases complicated by cognitive deficits. DESIGN, TIME AND SETTING: A non-randomized, concurrent, case-control investigation was performed in three medical centers in the Czech Republic (Department of Neurology at the University Hospital in Hradec Kralove, Department of Neurology at the 2rd Medical Faculty, and the University Hospital Motol) between October 2000 and November 2006. PARTICIPANTS: Eighteen patients with probable AIzheimer's disease, 4 patients with Creutzfeldt-Jakob disease, 10 patients with frontotemporal dementia, 9 patients with clinically isolated syndrome suggestive of multiple sclerosis, and 7 patients with multiple sclerosis, as well as 38 race-, nationality-, and age-matched cognitively intact controls, were included in the study. Diagnoses were established based on the following criteria: the criteria for Alzheimer's disease proposed by the National Institute of Neurological and Communicative Disorders and Stroke/Alzheimer's Disease and Related Disorders Association, WHO criteria for Creutzfeldt-Jakob disease, Neary criteria for frontotemporal dementia, and McDonald's criteria for multiple sclerosis. All included patients were confirmed to suffer from various degrees of dementia. METHODS: Enzyme-linked immunosorbent assay was used to measure concentrations of tau-protein, phosphorylated tau-protein, and Aβ42 in cerebrospinal fluid (CSF) samples collected by standard lumbar puncture from each patient. Moreover, 14-3-3 protein was assessed by Western blot in CSF of Creutzfeldt-Jakob disease patients. Cognitive status was assessed using the Mini Mental Scale Examination (MMSE) in all subjects. MAIN OUTCOME MEASURES: Establishment of biomarkers with greatest specificity and sensitivity for the investigated disorders according to Receiver Operating Characteristic curves, which were based on values from patients and controls; correlation between concentrations of given biomarkers and demographic parameters, diagnosis, duration of disease, and level of cognitive deficit. RESULTS: Increased concentrations of total tau protein and phosphorylated tau protein, and decreased levels of Aβ42, in CSF of Alzheimer's disease patients reached the required sensitivity/specificity ratio of 80% or greater. A marked elevation in CSF concentrations of total tau protein showed even greater sensitivity than 14-3-3 protein in Creutzfeldt-Jakob disease. There was no association between selected biomarkers and frontotemporal dementia or multiple sclerosis. Phosphorylated tau-protein was the only biomarker that noticeably correlated with MMSE scores for Alzheimer's disease.CONCLUSION: Levels of total tau protein, phosphorylated tau protein, and A!342 in the CSF could differentiate patients with Alzheimer's disease and Creutzfeldt-Jakob disease from healthy controls and patients with other neurodegenerative disorders. The diversity of absolute values demonstrates the necessity to establish a specific standard for each laboratory. 展开更多
关键词 Alzheimer's disease Creutzfeldt-Jakob disease multiple sclerosis beta-amyloid 42 total tau protein phosphorylated tau protein
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New and Newer Vaccines for SARS-CoV-2 Variants. Are the Major Vaccine Developers on the Right Track, Or Is Delipidation the Answer?
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作者 B. E. Cham 《Advances in Infectious Diseases》 2021年第2期165-170,共6页
Background: Global Covid-19 pandemic has led to remarkable scientific achievements resulting in the development and rapid implementation of vaccines towards the original wild-type SARS-CoV-2 virus. Most Covid-19 vacci... Background: Global Covid-19 pandemic has led to remarkable scientific achievements resulting in the development and rapid implementation of vaccines towards the original wild-type SARS-CoV-2 virus. Most Covid-19 vaccines are targeted to only one protein (the Spike protein) on the virus. SARS-CoV-2 that causes Covid-19 naturally undergoes multiple mutations over time. Such mutations can be inconsequential or have dire consequences. The lack of effectiveness of current vaccines towards mutated variants of Covid-19 is of major concern. The objective of this study is to describe an optimal solvent system that creates, via delipidation, a non-synthetic, host-derived or nonhost-derived modified viral particle that has its lipid envelope removed, exposing hidden undenatured proteins from within the virus, that generate a positive immunologic response when administered into a host, thereby providing a vaccine that offers strong and broad protection against the virus. Methods: Lipid removal from viruses by specific procedures renders the exposure of hidden proteins. Protection by antibodies to all of the virus’ protein types has shown to be far superior to protection by antibodies that are created by a single protein type. Results: Published studies with the Hepatitis virus, Pestivirus and HIV virus have reported the wide range of applications with this delipidation approach resulting in effectively long-term and broad protection vaccines. Conclusion: Mutations are rendering existing vaccines less effective. New approaches to obtain a more permanent vaccine that minimizes the effects of mutation are obtainable by delipidation of the viral particle and thereby creating vaccines that are more permanent with broad protection. 展开更多
关键词 SARS-CoV-2 Covid-19 Spike Protein Variant Mutation VACCINE Virus BUTANOL Diisopropylether Delipidation total Protein
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Serum Protein Level Is Related to ADL in Patients with Parkinson’s Disease
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作者 Lihe Huang Xuezhen Deng +3 位作者 Jieyi Yang Xuemei He Yanchun Zhu Lijian Chen 《Open Journal of Nursing》 2022年第11期738-744,共7页
Objectives: To establish an ADL prediction model for Parkinson’s inpatients as an auxiliary evaluation scheme. Methods: The data of Parkinson’s patients hospitalized in the Department of Neurology of Affiliated Brai... Objectives: To establish an ADL prediction model for Parkinson’s inpatients as an auxiliary evaluation scheme. Methods: The data of Parkinson’s patients hospitalized in the Department of Neurology of Affiliated Brain Hospital of Guangzhou Medical University from 2019 to 2022, which suited the criteria were collected, and a multiple linear regression model was established with serum total protein, serum albumin, age, BMI and education level as independent variables and BI scores as dependent variables. Results: A total of 95 PD patients were included (mean 70.05 ± 10.87 years): 53 males and 42 females. The correlation analysis showed that the serum total protein (r = 0.398, P Conclusion: The ADL multiple linear regression model can be used as an important means to evaluate the ADL ability of PD patients in hospital. 展开更多
关键词 Parkinson’s Disease NURSING Serum total Protein Serum Albumin Activities of Daily Living
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Morphological and biochemical responses of Oryza sativa L.(cultivar MR219) to ion beam irradiation 被引量:5
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作者 Anna Pick Kiong LING Ying Chian UNG +3 位作者 Sobri HUSSEIN Abdul Rahim HARUN Atsushi TANAKA Hase YOSHIHIRO 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2013年第12期1132-1143,共12页
Objective: Heavy ion beam, which has emerged as a new mutagen in the mutation breeding of crops and ornamental plants, is expected to result in the induction of novel mutations. This study investigates the morphologic... Objective: Heavy ion beam, which has emerged as a new mutagen in the mutation breeding of crops and ornamental plants, is expected to result in the induction of novel mutations. This study investigates the morphological and biochemical responses of Oryza sativa toward different doses of carbon ion beam irradiation. Methods: In this study, the dry seeds of O. sativa were irradiated at 0, 20, 40, 60, 80, 100, and 120 Gy, followed by in-vitro germination under controlled conditions. Morphological and biochemical studies were conducted to investigate the morphological and physiological responses of O. sativa towards ion beam irradiation. Results: The study demonstrated that low doses(10 Gy) of ion beam have a stimulating effect on the height, root length, and fresh weight of the plantlets but not on the number of leaves. Meanwhile, doses higher than 10 Gy caused reductions in all the morphological parameters studied as compared to the control samples. The highest total soluble protein content [(2.11±0.47) mg/g FW] was observed in plantlets irradiated at 20 Gy. All irradiated plantlets were found to have 0.85% to 58.32% higher specific activity of peroxidase as compared to the control samples. The present study also revealed that low doses of ion beam(10 and 20 Gy) had negligible effect on the total chlorophyll content of O. sativa plantlets while 40 Gy had a stimulating effect on the chlorophyll content. Plantlets irradiated between 40 to 120 Gy were shown to be 0.38% to 9.98% higher in total soluble nitrogen content which, however, was not significantly different from the control samples. Conclusions: Carbon ion beam irradiation administered at low to moderate doses of 10 to 40 Gy may induce O. sativa mutants with superior characteristics. 展开更多
关键词 In vitro mutagenesis Ion beam irradiation total chlorophyll content total soluble protein content Mutation breeding
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Kallikrein-related peptidases 6 and 10 are elevated in cerebrospinal fluid of patients with Alzheimer’s disease and associated with CSF-TAU and FDG-PET 被引量:3
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作者 Oliver Goldhardt Inanna Warnhoff +7 位作者 Igor Yakushev Ilijana Begcevic Hans Förstl Viktor Magdolen Antoninus Soosaipillai Eleftherios Diamandis Panagiotis Alexopoulos Timo Grimmer 《Translational Neurodegeneration》 SCIE CAS 2019年第1期304-316,共13页
Background:Alterations in the expression of human kallikrein-related peptidases(KLKs)have been described in patients with Alzheimer’s disease(AD).We elucidated the suitability of KLK6,KLK8 and KLK10 to distinguish AD... Background:Alterations in the expression of human kallikrein-related peptidases(KLKs)have been described in patients with Alzheimer’s disease(AD).We elucidated the suitability of KLK6,KLK8 and KLK10 to distinguish AD from NC and explored associations with established AD biomarkers.Methods:KLK levels in cerebrospinal fluid(CSF),as determined by ELISA,were compared between 32 AD patients stratified to A/T/(N)system with evidence for amyloid pathology and of 23 normal controls with normal AD biomarkers.Associations between KLK levels and clinical severity,CSF and positron emission tomography(PET)based AD biomarkers were tested for.Results:Levels of KLK6 and KLK10 were significantly increased in AD.KLK6 differed significantly between AD A+/T+/N+and AD A+/T−/N+or NC with an AUC of 0.922.CSF pTau and tTau levels were significantly associated with KLK6 in AD.Conclusions:KLK6 deserves further investigations as a potential biomarker of Tau pathology in AD. 展开更多
关键词 Alzheimer’s disease(AD) Kallikrein-like peptidase(KLK) KLK6 KLK8 KLK10 Cerebral amyloid load Cerebrospinal fluid(CSF) Amyloid 1-42 Aβ1-42 Aβ42 Tau protein total tau tTau Phospho tau pTau Positron emission tomography(PET)
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A comparative study to evaluate the effect of limited access dressing on diabetic ulcers
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作者 Thittamaranahalli Muguregowda Honnegowda Pramod Kumar +6 位作者 Krishnananda Prabhu Ashwini Kumar Pragna Rao E.G.Padmanabha Udupa Shobha Kamath Antony Sylvan D’Souza Krishna Kishore Mahato 《Plastic and Aesthetic Research》 2015年第1期266-271,共6页
Aim:Emerging evidence favors the important role of antioxidants,matrix metalloproteinases(MMPs),and nitric oxide(NO)in the healing of diabetic wounds.There is a lack of substantial evidence regarding the effects of ne... Aim:Emerging evidence favors the important role of antioxidants,matrix metalloproteinases(MMPs),and nitric oxide(NO)in the healing of diabetic wounds.There is a lack of substantial evidence regarding the effects of negative pressure on antioxidants,MMPs and NO in chronic wounds associated with diabetes.Methods:A total of 55 type 2 diabetic patients with leg ulcers were divided into 2 groups:a limited access dressing(LAD)group(n=27)and a conventional dressing group(n=28).Levels of hydroxyproline,total protein,MMP-2 and MMP-9,NO and antioxidants including reduced glutathione(GSH)and the oxidative biomarker malondialdehyde(MDA)were measured in the granulation tissue at days 0 and 10.Changes in levels between the LAD and conventional groups were determined by the Student’s t-test.Results:After 10 days of treatment,the LAD vs.conventional dressing group showed increase in the levels of hydroxyproline(mean±standard deviation=55.2±25.1 vs.29.2±1,P<0.05),total protein(12.8±6.5 vs.8.34±3.2,P<0.05),NO(1.13±0.52 vs.0.66±0.43,P<0.05),GSH(7.0±2.4 vs.6.6±2.2,P<0.05)and decrease in MMP-2(0.47±0.33 vs.0.62±0.30,P<0.05),MMP-9(0.32±0.20 vs.0.53±0.39,P<0.05)and MDA(6.8±2.3 vs.10.4±3.4,P<0.05).Conclusion:When compared to conventional dressings,LAD induces biochemical changes by significantly increasing the levels of hydroxyproline,total protein,NO and antioxidants levels,and significantly reducing MMPs(MMP-2 and MMP-9)and an oxidative biomarker in diabetic wounds.These biochemical changes are thought to favor diabetic wound healing. 展开更多
关键词 ANTIOXIDANTS diabetic wounds HYDROXYPROLINE limited access dressing matrix metalloproteinases nitric oxide reactive oxygen species and oxidative stress biomarker total protein
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