Matching traditional Chinese medicine and western medicine-based research:Advanced nutraceutical development for proactive gastric cancer prevention

Gastric cancer(GC),the third leading cause of cancer-related death globally,is complex and heterogeneous.This review explores multidisciplinary investigations of traditional Chinese medicine(TCM)combined with Western medical practices,emphasizing the development of nutraceuticals for cancer prevention.Using advanced analytical chemistry and food chemistry techniques,this study investigated how TCM components may be optimized for nutraceutical development.Focusing on molecular interactions with GC pathways,particularly the NF-κB,PI3K/Akt,and Wnt/β-catenin pathways,we examined the effects of TCM polyherbal formulas,extracts,and isolated compounds.These agents modulate apoptosis and cellular proliferation,underscoring their potential in preventive strategies.The convergence of nutraceutical and medicine food homology studies highlights a significant shift towards integrating TCM-derived compounds in a preventive health framework.This approach aims not only to enhance efficacy and reduce side effects but also to champion a preventive paradigm using personalized medicine to advance proactive health maintenance and disease prevention.The combination of TCM and western medical practices offers promising avenues for future research and practical applications in GC prevention.

Application of Classical Prescriptions of Rhizome Chinese Herbal Medicines for Medicine and Food Homology and Development Status of Its Modern Food

The efficacy,application and compatibility of five kinds of Chinese Herbal Medicines medicine(including yam,licorice,Platyc-odonisi Radix,Polygonati Rhizoma,and Pueraria Lobata)for medicine and food homology in the past five years were systematically searched and summarized,and the current situation of their food development was reviewed to provide theoretical basis for the research and development of this kind of CHMs for health care.

Determination of vanadium in food and traditional Chinese medicine by graphite furnace atomic absorption spectroscopy

Various experimental conditions were described for the vanadium determinationby graphite furnace atomic absorption spectroscopy (GFAAS). The experiments showed that whenatomization took place under the conditions where the combination of a pyrolytic coating graphitetube and fast raising temperature were used and the temperature was stable, the signal peak shapescould be improved, the sensitivity was enhanced, and the memory effect was removed. The vanadium infood and traditional Chinese medicinal herbs can be accurately determined using the standard curvemethod.

Approaches and Perspectives of the Westward Spread of Traditional Chinese Medicine: A Case Study of the Radicis Chynae

Some Western scholars have re-examined the concept of“Chinese medicine”and its knowledge system under the influence of global history research methods in recent years,in an attempt to understand the factors that led to the spread of Chinese medicine around the world,and what kind of Chinese medicine is constituted outside of China.Thus,researchers have studied the initial stage of traditional Chinese medicine(TCM)’s entry into the western world,tracing its roots and observing new knowledge systems formed in the process of cross-cultural communication.Responding to the research of Western scholars,this paper studies the Radicis Chynae(《中国根书简》Letters on Chinese Root),a monograph written by Andreas Vesalius,a famous European anatomist who lived in the 16th century.The author of this article examined the understanding and interpretation of Chinese medicine by the European intelligentsia from 16th to 19th century,investigated the influence of the westward spread of TCM on the scientific revolution and medical progress in Europe,and analyzed its relationship with the rise of Sinology in Europe.This article discusses the knowledge interaction between Chinese medicine and the formation of modern European medicine from the perspective of global history and cross-culture.

East to west:research progress in traditional Chinese medicine for antiaging strategies

Delaying the onset of aging is considered an important tool for dealing with the global crisis of the aging population.Accordingly,several antiaging studies have been conducted using strategies of modern medicine and traditional Chinese medicine(TCM).In this review,we summarize the current state of antiaging research related to both modern medicine and TCM and provide suggestions for further research.Various theories related to the aging process have been proposed,including the free radical theory,mitochondrial DNA damage theory,telomere theory,cross-linking theory,and intestinal flora theory,and a number of antiaging chemical treatments have been developed using modern medicine.These theories and achievements have resulted in major advancements in antiaging research.TCM is based on practical experience and has a long history with unique advantages in antiaging research.Some antiaging practices such as moderate diet consumption,acupuncture,Tai Chi(a traditional Chinese martial art that can relax the nerves to slow down the aging process),and Wuqin Xi(a traditional Chinese exercise to strengthen the body)have been adopted to maintain health.Several TCMs and prescriptions,particularly those related to medicine food homology,have been used in antiaging treatment,and their antiaging properties have been demonstrated to be effective based on pharmacological experiments and clinical applications.

Introducing Chinese food therapy： a review of origin, developing course and modern application

Objective： This review introduced the origin, developing course and modern application of Chinese food therapy （CFT） in traditional Chinese medicine to enrich our understanding of relevant concepts and ways. Methods： PubMed, EMBASE, CBM, CNKI, VIP and Wanfang Databases were searched for papers on a range of terms relating to CFT. 41 references were finally selected for this review. Results： The development of CFT is comprised of five important periods in Chinese history. They axe as following： the infancy period represented by the idea of homogeny of medicine and food; theoretical development period represented by Inner Canon of Yellow Emperor, Shennong 1＇ Classic of Materia Medica and Treatise on Febrile and Miscellaneous Diseases; specialized volumes on CFT represented by Dietotherapy Worth a Thousand Gold; representative monographs on CFT represented by Dietetic Materia Medica and Principles of Correct Diet and the perfect period represented by integration of ancient food therapy practice and modern nutriology. Conclusion： CFT harbors a long and profound history, making great contribution to people＇s health maintenance and illness prevention. With the further development of integrative medicine, CFT may play a more important role in clinics and family health care based on integration of syndrome differentiation and disease differentiation.

Puerarin ameliorates allodynia and hyperalgesia in rats with peripheral nerve injury

Puerarin is a major active ingredient of the traditional Chinese plant medicine,Radix Puerariae,and commonly used in the treatment of myocardial and cerebral ischemia.However,the effects of puerarin on neuropathic pain are still unclear.In this study,a neuropathic pain animal model was created by partial sciatic nerve ligation.Puerarin（30 or 60 mg/kg） was intraperitoneally injected once a day for 7 days.Mechanical allodynia and thermal hyperalgesia were examined at 1 day after model establishment.Mechanical threshold and paw withdrawal latency markedly increased in a dose-dependent manner in puerarin-treated rats,especially at 7 days after model establishment.At 7 days after model establishment,quantitative real-time reverse transcriptase-polymerase chain reaction results showed that puerarin administration reversed m RNA expression of transient receptor potential vanilloid 1（Trpv1） and transient receptor potential ankyrin 1（Trpa1） in a dose-dependent manner in dorsal root ganglion neurons after peripheral nerve injury.These results suggest that puerarin dose-dependently ameliorates neuropathic pain by suppressing Trpv1 and Trpa1 up-regulation in dorsal root ganglion of neuropathic pain rats.

Reduction in nerve root compression by the nucleus pulposus after Feng's Spinal Manipulation

Ninety-four patients with lumbar intervertebral disc herniation were enrolled in this study. Of these, 48 were treated with Feng＇s Spinal Manipulation, hot fomentation, and bed rest （treatment group）. The remaining 46 patients were treated with hot fomentation and bed rest only （control group）. After 3 weeks of treatment, clinical parameters including the angle of straight-leg raising, visual analogue scale pain score, and Japanese Orthopaedic Association score for low back pain were improved. The treatment group had significantly better improvement in scores than the control group. Magnetic resonance myelography three-dimensional reconstruction imaging of the vertebral canal demonstrated that filling of the compressed nerve root sleeve with cerebrospinal fluid increased significantly in the treatment group. The diameter of the nerve root sleeve was significantly larger in the treatment group than in the control group. However, the sagittal diameter index of the herniated nucleus pulposus and the angle between the nerve root sleeve and the thecal sac did not change significantly in either the treatment or control groups. The effectiveness of Feng＇s Spinal Manipulation for the treatment of symptoms associated with lumbar intervertebral disc herniation may be attributable to the relief of nerve root compression, without affecting the herniated nucleus pulposus or changing the morphology or position of the nerve root.

Photoprotective Effects of Hydroxychloroqine and TCMs on Human Keratinocytes Damaged from Ultraviolet Irradiation

Objective: To investigate damage effects of ultraviolet irradiation on eternal keratinocyte-HaCaT cells and to evaluate photo-protective efficiency of hydroxychloroqine and Traditional Chinese Medicines(epigallocatechingallate[EGCG], baikal skullcap root and szechwan lovge rhizome) on HaCaT cells damaged by middle wave ultraviolet(UVB) irradiation. Methods: Subconfluent HaCaT cells were sham or UVB irradiated and treated with above TCM agents. The damage degree of HaCaT cells was observed by a light microscop. Cell growth was recorded by cell count and cellular activity was detected by MTT method. The secretion amount of IL-6 and TNF-α was measured by ELISA. Results: The irradiation damage of HaCaT cells was depended on the irradiated dosages and cellular activity was reduced by 36%-80%, with a maximum decrease over 90% after 72 h. The intervention of the above drugs may increase the cellular activity by 10%-72%. The photo-protective efficiency was more apparent in EGCG (from 1.19±0.07 to 1.28±0.06, P<0.01) than that in hydroxychloroqine (from 0.43±0.04 to 0.96±0.04, P<0.05). The other two tested drugs also showed photo-protective effect(from 0.44±0.07 to 1.21±0.02, P<0.05). As to cytokine secretion, EGCG could decline the secretion amount of IL-6 and TNF-α apparently. Hydroxychloroqine and baikal skullcap root baikal skullcap root could only reduce the secretion of IL-6. The secretion of IL-6 and TNF-α could not be inhibited by szechwan lovge rhizome. Conclusion: The injury effect of UVB irradiation on cultured keratinocytes is dose-dependent and the tested drugs have photo-protective potency. Inhibition of cytokine secretion may be one of the mechanisms related to reducing the damage effect of UVB irradiation.

Application and research advancement on the microwave-assisted extraction

This paper summarized application and research advancement of the microwave-assisted extraction in the agriculture, food industry, environmental analytical chemistry, traditional Chinese medicine industry, and so on. The microwave-assisted extraction was manifested to be a simple device, wide area of application, high extraction efficiency, good reproducibility and low consumption of agent and time as well as low environmental pollution. At present, industrialization question of the microwave-assisted extraction technology has been attached importance, which will impel the microwave-assisted extraction technology to more development in the future

The dynamic changes and mechanisms of Rehmanniae radix processing based on Maillard reaction

Background:Traditional Chinese medicines are usually processed before they are used for clinical treatment.This is done in a way associated with the Maillard reaction.Methods:Based on the Maillard reaction,this paper analyzed the degree of processing of rehmannia root(Rehmanniae radix)relative to the dynamic variation rules of Maillard reaction index parameters,including pH,A420,amino acids,and 5-hydroxymethylfurfural.Furthermore,this study introduced thermal analysis techniques and pyrolysis kinetics to assess the influence of the correlation between processing raw rehmannia root and the Maillard reaction during carbonization.It then went through the whole process of transforming the raw material to end-product in order to explain the scientific connotation of processing it.Results:The results showed that each time rehmannia root was processed,its pH value and amino acid content decreased,while the A420 value and 5-HMF increased.Processing with wine shows a significant difference in these experimental indexes.The position and intensity of the maximum thermal weight loss rate peak of processed rehmannia root at different degrees of processing are different.Comprehensive quantitative 221±0.2°C for processed rehmannia root carbonization was the processing temperature limit.Moreover,the kinetic solution verified that the activation energy corresponding to the carbonization temperature was close to the maximum value of the activation energy of the whole carbonization process,and the optimal mechanism function was g(α)=((1−α)−1/3−1)2.Conclusion:The Maillard reaction occurred during the processing of rehmannia root mixed with carbonization.With each increase of the number of steaming and drying cycles involved in the processing,the level of Maillard reaction increased significantly.The wine-steaming method had a significant effect on the quality of the processed product.

The role and status of fuzheng in cancer prevention and treatment

Traditional Chinese medicine(TCM) treatment of cancer has a long history, and is an important part of cancer prevention and control in China. Fuzheng, also called reinforcing healthy qi and supplementing the root, is the most fundamental principle of TCM in cancer prevention and control. In recent decades, this treatment has been thoroughly studied and widely applied, and played a crucial role in cancer prevention and treatment. With regard to the treatment of malignant tumors, Chinese medicine is mainly used in the following areas: improving symptoms, enhancing the quality of life, reducing postoperative recurrence and metastasis, increasing efficacy and decreasing toxicity together with radiotherapy and chemotherapy treatments, and to some extent prolonging the survival of advanced tumors.

基于CiteSpace和VOSviewer对药食同源中药山楂相关研究的可视化分析

目的分析中药山楂在中医药领域发表的相关文献,梳理该药的研究现状、研究热点及发展趋势。方法计算机检索中国知网和Web of Science数据库,导出1994年1月至2023年12月主题为山楂的中医药领域中英文文献。使用CiteSpace和VOSviewer软件对年发文量,发文作者、机构,文献被引频次,关键词共现、聚类和突现等进行可视化分析。结果共纳入文献2526篇,其中中文文献2301篇,英文文献225篇。中英文文献的年发文量呈波动上升趋势,发文量最多的作者分别是邵峰和Hosseinimehr Seyed Jalal,发表量最大的机构分别是北京中医药大学和University of Michigan。被引频次较多的中文文献主要为山楂的化学成分、药理作用等方面的研究,英文文献主要为山楂活性成分抗氧化作用方面的研究。中文文献高频关键词为山楂、高脂血症等,英文文献高频关键词为山楂、黄酮类化合物等。中文关键词共聚为13类,英文关键词共聚为18类。中文文献有19个突现词,英文文献有14个突现词。结论山楂的临床研究、有效成分测定及药理作用和基于数据挖掘的研究受到较多关注。山楂单体成分治疗疾病的作用和机制、山楂治疗癌症的有效成分和机制有待进一步研究。

Electroacupuncture upregulated platelet derived growth factor expression in spared dorsal root ganglion of cats

A bilateral spared dorsal root ganglion model was established in healthy adult cats by bilateral resection of L1-5 and L7-S2 dorsal root ganglia. L6 dorsal root ganglia were spared. Zusanfi （ST36） and Xuanzhong （BL39） or Futu （ST32） and Sanyinjiao （SP6） were alternatively electro-stimulated on the right leg. Immunohistochemical staining of anti-serum platelet-derived growth factor demonstrated that the number of total neurons and medium-small sized platelet-derived growth factor positive neurons was significantly decreased on the 7~ day following injury. After 7 days of acupuncture, the total number of positive and large neurons staining for platelet-derived growth factor on the acupuncture side significantly increased compared to the non-acupuncture side. After acupuncture for 14 days, the total positive and medium-small sized neurons significantly increased compared with the non-acupuncture side. Results indicate that acupuncture promoted the synthesis of platelet-dedved growth factor in spared dorsal root ganglia.

Actinidia chinensis Planch.root extract downregulates the Wnt/β-catenin pathway to treat gastric cancer:a mechanism study based on network pharmacology

Background:Actinidia chinensis Planch.roots(AcRoots)have been applied as an anti-inflammatory and antitumor drug in the treatment of gastric cancer(GC).However,their mechanisms against GC cells remain unclear.To investigate the anticancer effect of AcRoots in GC and the possible underlying mechanism by using network pharmacology.Methods:Differentially expressed genes between gastric precancerous lesions and cancer were analyzed in Gene Expression Omnibus datasets,and these genes were overlapped with potential targets of AcRoots.Potential targets and pathways for AcRoots treatment of GC predicted by network pharmacology.Furthermore,we used the GC cell line HGC27 to explore the molecular mechanisms in the context of hub genes in apoptosis,invasion,metastasis,and epithelial to mesenchymal transition-promoting factors.Molecular docking between hub targets and active drug components was also performed.Results:Network pharmacological analysis suggested that the potential mechanism was related to the Wnt pathway and predicted nine hub genes.In in vitro studies,AcRoots significantly decreased HGC27 cell viability and promoted apoptosis by upregulating caspase3 and downregulating Bcl2.Moreover,it suppressed invasion and metastasis as well as the expression of epithelial to mesenchymal transition-related factors.In addition,AcRoots affected the phosphorylation level of GSK3β(Ser9)in the Wnt pathway to promote the degradation ofβ-catenin,resulting in the downregulation of the downstream target genes c-myc,cyclin D1 and snail.All the experimental results were consistent with the network pharmacology results.Conclusion:This study combined network pharmacology with in vitro experiments to provide valid evidence for the clinical promotion of AcRoots.

中药及保健食品中违禁添加7种降压类化学药物的HPLC-DAD法测定

建立了HPLC法同时测定中药及保健食品中可能违禁添加的7种降压类化学药物。采用C18色谱柱,以0.2%磷酸溶液-乙腈为流动相,梯度洗脱,检测波长220nm。阿替洛尔、盐酸可乐定、氢氯噻嗪、卡托普利、盐酸哌唑嗪、利血平和硝苯地平的线性范围分别为0.0284～2.84、0.029～2.9、0.053～5.3、0.054～5.45、0.054～5.4、0.034～3.38和0.031～3.12μg,回收率分别为99.7%、91.2%、99.5%、107.6%、108.7%、97.8%和106.1%,RSD分别为2.1%、3.5%、2.5%、3.8%、1.0%、4.2%和5.0%。

HPLC-DAD法同时检测镇静安神类中成药及保健食品中非法添加的药物

目的建立HPLC-DAD法同时检测镇静安神类中成药及保健食品中非法添加的9种安眠药物。方法样品分析采用Agilent Zorbax SB-Aq色谱柱（4.6 mm×250 mm,5μm）;流动相为含0.1%三乙胺的0.05 mol/L磷酸二氢铵-甲醇溶液,梯度洗脱;体积流量为1.0 m L/min;柱温（35±5）℃;检测波长分别为210 nm（盐酸多塞平、苯妥英钠、司可巴比妥钠、盐酸羟嗪）、220 nm（阿普唑仑、艾司唑仑、劳拉西泮）、254 nm（氯氮卓、盐酸氯丙嗪）,DAD全波长扫描（190~400 nm）。结果在147批药物（镇静安神类中成药87批、保健食品60批）中,11批检出盐酸多塞平,3批检出艾司唑仑,各有1批检出劳拉西泮、阿普唑仑和氯氮卓。结论该方法简便、快速、准确、重复性好,适用于基层药检机构检测非法添加药物。

基于PCA的中药黄芩药效评价方法研究

为实现基于谱效相关模式的中药黄芩的质量综合评价,采用主成分分析法(principal component analysis,PCA)对采集到的色谱指纹图谱和药效指标(抑菌率)的大量数据进行降维处理,然后建立中药黄芩谱效结合评价系统,测试结果证明了该方法的先进性与可靠性。文中的降维方法可以为中药材质量评价研究提供一种新的、可借鉴的思路和方法。

紫草油治疗压疮效果的Meta分析

目的综合评价紫草油治疗压疮的效果。方法计算机检索PubMed、MEDLINE、Web of Knowledge、CINAHL、Springer、EBSCO、中国生物医学文献数据库、中国知网、万方数据库、中国科技期刊数据库中使用紫草油治疗压疮的随机对照试验,由2名研究人员筛选和提取资料,运用RevMan 5.3软件进行Meta分析。结果共纳入16篇随机对照试验(761例患者)。Meta分析结果显示,有效率存在统计学意义[OR=5.48,95%CI(3.47,8.66),P<0.01],有效时间存在统计学意义[MD=-5.53,95%CI(-6.10,-4.65),P<0.01]。结论紫草油治疗压疮具有良好的效果,值得临床推广。

UPLC-MRM-IDA-EPI法测定中成药及保健食品中非法添加的5种激素

目的建立中成药及保健食品中非法添加的5种激素的快速测定方法。方法采用ACQUITY UPLC BEN C18色谱柱(50 mm×2.1 mm,1.7μm),流动相为甲醇-0.1%甲酸水溶液(60∶40),电喷雾离子源(ESI),多反应监测(MRM)联合相关信息扫描(IDA)与增强离子扫描(EPI)的扫描方式,对中成药及保健食品中非法添加的5种激素成分进行快速定性、定量分析。结果 4种雌激素(雌二醇、雌三醇、雌酮和己烯雌酚)及黄体酮在1~100μg·mL^-1线性关系良好,相关系数均大于0.999。样品平均回收率在97.2%~104.5%,RSD在1.5%~7.6%。结论该方法操作简单,检验速度快,定性可靠,定量准确,灵敏度高,适用于中成药及保健食品中非法添加激素的快速检测。