Since the outbreak of coronavirus disease 2019(COVID-19),the author has been involved in the treatment of COVID-19 in the first line.It is found that some patients had a long viral nucleic acid negative time,and even ...Since the outbreak of coronavirus disease 2019(COVID-19),the author has been involved in the treatment of COVID-19 in the first line.It is found that some patients had a long viral nucleic acid negative time,and even the course of the disease lasted more than 40 days.After a thorough investigation of the root causes,it is found the four possible causes were:"Blood stasis syndrome"is difficult to remove;"Damp heat"sticky greasy;Stubborn basic diseases;weak constitution.All the patients treated with traditional Chinese medicine have turned negative,and now the typical cases were analyzed and summarized in order to provide reference for the traditional Chinese medicine treatment and scientific research of the patients with COVID-19 who were difficult to turn negative.展开更多
Objective:To use high-frequency ultrasound to evaluate the efficacy ofα-lipoic acid combined with traditional Chinese medicine in the treatment of diabetic peripheral neuropathy(DPN),so as to provide a basis for clin...Objective:To use high-frequency ultrasound to evaluate the efficacy ofα-lipoic acid combined with traditional Chinese medicine in the treatment of diabetic peripheral neuropathy(DPN),so as to provide a basis for clinical medication and evaluation of therapeutic effect.Methods:From December 2018 to December 2019,110 patients with DPN who met the inclusion and exclusion criteria were divided into observation group and control group according to different treatment plans.The control group was treated withα-lipoic acid,and the observation group was treated withα-lipoic acid combined with traditional Chinese medicine encapsulation treatment,and used high-frequency ultrasound,nerve conduction velocity and serological examination to comprehensively evaluate the effectiveness of the two treatment methods.Results:The mean amplitude of glycemic excursions(MAGE)value of the observation group and the control group after treatment was significantly lower than before treatment(P<0.05);After treatment,the SCV of the ulnar nerve,median nerve,and common peroneal nerve in the two groups was significantly faster than before treatment(P<0.05).Similar to SCV,the MCV of the three nerves measured after treatment in the two groups was significantly faster than before treatment(P<0.05).Compared between the groups after treatment,the three kinds of nerve SCV and MCV in the observation group were significantly faster than those in the control group(P<0.05).The cross-sectional area(CSA)value of the ulnar nerve,median nerve and common peroneal nerve in the observation group after treatment was significantly lower than before treatment(P<0.05).Compared with the control group after treatment,the three nerves CSA in the observation group was significantly lower than that in the control group(P<0.05).Abnormal ultrasound performance:The proportion of abnormal ultrasound performances of the ulnar nerve,median nerve,and common peroneal nerve in the observation group after treatment was significantly lower than before treatment(P<0.05).The composition ratio of internal echo reduction and ambiguity in the observation group after three nerve treatments was significantly lower than that in the control group after treatment(P<0.05).In the correlation analysis,the three kinds of nerve CSA before and after treatment were negatively correlated with SCV and amplitude(P<0.05),and positively correlated with latency(P<0.05).Conclusion:The combination ofα-lipoic acid and Chinese medicine encapsulation technology has a good effect on the treatment and repair of DPN nerve damage,and can be routinely applied in clinical treatment.High-frequency ultrasound can intuitively observe changes in peripheral nerves and can be used to evaluate the prognosis of DPN.展开更多
Disorder of energy metabolism is a major pathological change in the progression of heart failure.This process leads to insufficient myocardial energy and further aggravates cardiac dysfunction.Disorders of metabolic s...Disorder of energy metabolism is a major pathological change in the progression of heart failure.This process leads to insufficient myocardial energy and further aggravates cardiac dysfunction.Disorders of metabolic substrate utilization,mainly glucose and fatty acids,play an important role in this process.Research over the years has shown that some traditional Chinese medicines or compound prescriptions whose main role is to replenish qi and warm yang have good effects in regulating energy metabolism disorders.It has been found that some active ingredients in traditional Chinese medicine can regulate the uptake and utilization of myocardial cell metabolic substrate,so that the metabolism of myocardial cells can be adjusted in a direction that is beneficial to the body under hypoxic conditions,increasing the overall energy supply of the myocardium and improving heart function.This article reviewed the research of traditional Chinese medicine intervention on glucose and lipid metabolism of heart failure myocardial cells,and preliminarily summarizes the law and mechanism of traditional Chinese medicine intervention in heart failure myocardial glucose and fatty acid metabolism,hoping to provide clues for energy metabolism therapy research from the perspective of traditional Chinese medicine.展开更多
AIM:To assess the antacid effects of the tonic Chinese herbal prescriptions,Si-Jun-Zi-Tang(SJZT) and Shen-Ling-Bai-Zhu-San(SLBZS).METHODS:Decoctions of the tonic Chinese herbal prescriptions,SJZT and SLBZS,were prepar...AIM:To assess the antacid effects of the tonic Chinese herbal prescriptions,Si-Jun-Zi-Tang(SJZT) and Shen-Ling-Bai-Zhu-San(SLBZS).METHODS:Decoctions of the tonic Chinese herbal prescriptions,SJZT and SLBZS,were prepared according to Chinese original documents.The pH of the prescription decoctions and their neutralizing effects on artif icial gastric acids were determined and compared with water and the active controls,sodium bicarbonate and colloidal aluminum phosphate.A modified model of Vatier's artificial stomach was used to determine the duration of consistent neutralization effect on artificial gastric acids.The neutralization capacity in vitro was determined with the titration method of Fordtran's model.RESULTS:The results showed that both SJZT and SLBZS have antacid effects in vitro.Compared with the water group,SJZT and SLBZS were found to possess signif icant gastric acid neutralizing effects.The duration for consistent neutralization of SLBZS was signif icantly longer than that of water.Also,SLBZS and SJZT exhibited signif icant antacid capacities compared to water.CONCLUSION:SJZT and SLBZS were consistently active in the artificial stomach model and are suggested to have antacid effects similar to the active control drugs.展开更多
Wistar rats were intragastrical y perfused with Chinese medicines used for tonifying the kidney. These included 0.180 g/mL of Herba Epimedi （Epimedium）, Semen Cuscutae （Dodder Seed）, or Herba Cistanches （Desertli...Wistar rats were intragastrical y perfused with Chinese medicines used for tonifying the kidney. These included 0.180 g/mL of Herba Epimedi （Epimedium）, Semen Cuscutae （Dodder Seed）, or Herba Cistanches （Desertliving Cistanche）, 0.04 mg/mL monoamine oxidase-B inhibitor selegiline, or distil ed water for 14 consecutive days to prepare drug-containing serum or blank serum. MES23.5 cells in the logarithmic phase were cultured in media supplemented with 15%drug-containing serum for 24 hours, fol owed by incubation in culture solution containing 100μmol/L H2O2 for 3 hours. 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） and flow tometry results showed that al drug-containing serums improved the survival rate of H 2 O 2-injured MES23.5 cells, inhibited pro-apoptotic FasL and caspase-3 expression, promoted anti-apoptotic Bcl-2 expression. However, drug-containing serums had little influence on Fas expression in H 2 O 2-injured MES23.5 cells. Enzyme-linked immunosorbent assay results showed that serum containing Herba Cistanches or Herba Epimedi increased the expression of nerve growth factor, brain-derived neurotrophic factor, and glial cellline-derived neurotrophic factor in injured MES23.5 cells;serum containing Semen Cuscutae only increased brain-derived neurotrophic factor expres-sion; while expression of the above neurotrophic factors remained the same in cells treated with serum containing selegiline. These findings indicate that Chinese medicines used to tonify the kid-ney can protect nerve cells by regulating the expression of apoptosis-related factors and neuro-trophic factors in MES23.5 cells.展开更多
Objective Our objective was to screen drugs with good protective effects on gefitinib-induced hepatotoxicity.Methods Fifty-four specific pathogen-free-grade male mice of the Institute of Cancer Research were randomly ...Objective Our objective was to screen drugs with good protective effects on gefitinib-induced hepatotoxicity.Methods Fifty-four specific pathogen-free-grade male mice of the Institute of Cancer Research were randomly divided into a normal group,gefitinib group,glutathione group,ligustrazine group,silymarin group,glycyrrhizic acid group,baicalin group,paeoniflorin group,and matrine group,with six mice in each group.Except for the normal group,the remaining groups of mice were intragastrically administered 400 mg·kg^(-1)of gefitinib for 16 days to induce liver injury.Mice in each treatment group were intragastrically administered 100 mg·kg^(-1)of the corresponding drug 30 minutes after gefitinib administration each day.The normal group and model group mice were intragastrically administered with an equal volume of 0.5%carboxy-methylcellulose sodium(CMC-Na),and the administration volume was 10 mg·kg^(-1).Then,30 minutes after the last administration,blood was collected from the retro-orbital venous plexus,and the mice were killed by cervical dislocation to obtain liver weight and calculate the liver index.Liver pathological changes were observed by hematoxylin–eosin(HE)staining;the levels of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in serum were detected using biochemical kits.AML12 cells were cultured in a medium containing drugs for 30 minutes.Except for the normal group,the remaining groups were induced cell damage with 20μmoL·L1 gefitinib for 24 hours.Cell viability was detected using a cell counting kit-8,and the levels of ALT,AST,and lactate dehydrogenase(LDH)in the cell culture supernatant were measured using biochemical kits.Results Animal experiments showed that compared with the gefitinib group,glycyr-rhizic acid and baicalin significantly increased the body weight of mice(p<0.01)and decreased the liver index and levels of ALT and AST(p<0.05);ligustrazine and silymarin significantly increased the body weight of mice(p<0.01)and decreased the level of AST(p<0.05);paeoniflorin and matrine significantly decreased the levels of ALT and AST(p<0.01).HE staining showed that the liver tissue of mice in the gefitinib group presented a large number of inflammatory cell infiltrations and disordered arrange-ment of hepatic cords;glutathione,glycyrrhizic acid,baicalin,paeoniflorin,and matrine significantly alleviated pathological damage to mouse liver tissue.Cell experi-ments showed that all drugs could alleviate gefitinib-induced damage to AML12 cells to varying degrees,among which glycyrrhizic acid,baicalin,and paeoniflorin had better protective effects,with cell survival rates increased to 96.4,81.1,and 78.2%,respectively.Compared with the gefitinib group,glycyrrhizic acid significantly reduced the levels of ALT,AST,and LDH(p<0.05);silymarin,baicalin,and paeoniflorin significantly reduced the levels of ALT and LDH(p<0.05).Conclusion Glycyrrhizic acid,baicalin,and paeoniflorin all have good protective effects against gefitinib-induced hepatotoxicity,among which glycyrrhizic acid has the best effect.展开更多
基金Science and Technology Research Project of Jiangxi Provincial Department of Science and Technology(No.S2020ZPYFG0006)Training Project of National Traditional Chinese Medicine Innovative Key Talents National Chinese Medicine(2019)(No.128)
文摘Since the outbreak of coronavirus disease 2019(COVID-19),the author has been involved in the treatment of COVID-19 in the first line.It is found that some patients had a long viral nucleic acid negative time,and even the course of the disease lasted more than 40 days.After a thorough investigation of the root causes,it is found the four possible causes were:"Blood stasis syndrome"is difficult to remove;"Damp heat"sticky greasy;Stubborn basic diseases;weak constitution.All the patients treated with traditional Chinese medicine have turned negative,and now the typical cases were analyzed and summarized in order to provide reference for the traditional Chinese medicine treatment and scientific research of the patients with COVID-19 who were difficult to turn negative.
基金National natural science foundation of China(No.81701891)Guided scientific research project of Shiyan science and technology bureau(No.18Y63)。
文摘Objective:To use high-frequency ultrasound to evaluate the efficacy ofα-lipoic acid combined with traditional Chinese medicine in the treatment of diabetic peripheral neuropathy(DPN),so as to provide a basis for clinical medication and evaluation of therapeutic effect.Methods:From December 2018 to December 2019,110 patients with DPN who met the inclusion and exclusion criteria were divided into observation group and control group according to different treatment plans.The control group was treated withα-lipoic acid,and the observation group was treated withα-lipoic acid combined with traditional Chinese medicine encapsulation treatment,and used high-frequency ultrasound,nerve conduction velocity and serological examination to comprehensively evaluate the effectiveness of the two treatment methods.Results:The mean amplitude of glycemic excursions(MAGE)value of the observation group and the control group after treatment was significantly lower than before treatment(P<0.05);After treatment,the SCV of the ulnar nerve,median nerve,and common peroneal nerve in the two groups was significantly faster than before treatment(P<0.05).Similar to SCV,the MCV of the three nerves measured after treatment in the two groups was significantly faster than before treatment(P<0.05).Compared between the groups after treatment,the three kinds of nerve SCV and MCV in the observation group were significantly faster than those in the control group(P<0.05).The cross-sectional area(CSA)value of the ulnar nerve,median nerve and common peroneal nerve in the observation group after treatment was significantly lower than before treatment(P<0.05).Compared with the control group after treatment,the three nerves CSA in the observation group was significantly lower than that in the control group(P<0.05).Abnormal ultrasound performance:The proportion of abnormal ultrasound performances of the ulnar nerve,median nerve,and common peroneal nerve in the observation group after treatment was significantly lower than before treatment(P<0.05).The composition ratio of internal echo reduction and ambiguity in the observation group after three nerve treatments was significantly lower than that in the control group after treatment(P<0.05).In the correlation analysis,the three kinds of nerve CSA before and after treatment were negatively correlated with SCV and amplitude(P<0.05),and positively correlated with latency(P<0.05).Conclusion:The combination ofα-lipoic acid and Chinese medicine encapsulation technology has a good effect on the treatment and repair of DPN nerve damage,and can be routinely applied in clinical treatment.High-frequency ultrasound can intuitively observe changes in peripheral nerves and can be used to evaluate the prognosis of DPN.
基金National Natural Science Foundation of China(NO.81960820)The Natural Science Foundation of Jiangxi(NO.20202BABL206141)。
文摘Disorder of energy metabolism is a major pathological change in the progression of heart failure.This process leads to insufficient myocardial energy and further aggravates cardiac dysfunction.Disorders of metabolic substrate utilization,mainly glucose and fatty acids,play an important role in this process.Research over the years has shown that some traditional Chinese medicines or compound prescriptions whose main role is to replenish qi and warm yang have good effects in regulating energy metabolism disorders.It has been found that some active ingredients in traditional Chinese medicine can regulate the uptake and utilization of myocardial cell metabolic substrate,so that the metabolism of myocardial cells can be adjusted in a direction that is beneficial to the body under hypoxic conditions,increasing the overall energy supply of the myocardium and improving heart function.This article reviewed the research of traditional Chinese medicine intervention on glucose and lipid metabolism of heart failure myocardial cells,and preliminarily summarizes the law and mechanism of traditional Chinese medicine intervention in heart failure myocardial glucose and fatty acid metabolism,hoping to provide clues for energy metabolism therapy research from the perspective of traditional Chinese medicine.
基金Supported by A Research Grant from Yen Kun Yin Cultural and Educational Foundation, Taipei, Taiwan, China
文摘AIM:To assess the antacid effects of the tonic Chinese herbal prescriptions,Si-Jun-Zi-Tang(SJZT) and Shen-Ling-Bai-Zhu-San(SLBZS).METHODS:Decoctions of the tonic Chinese herbal prescriptions,SJZT and SLBZS,were prepared according to Chinese original documents.The pH of the prescription decoctions and their neutralizing effects on artif icial gastric acids were determined and compared with water and the active controls,sodium bicarbonate and colloidal aluminum phosphate.A modified model of Vatier's artificial stomach was used to determine the duration of consistent neutralization effect on artificial gastric acids.The neutralization capacity in vitro was determined with the titration method of Fordtran's model.RESULTS:The results showed that both SJZT and SLBZS have antacid effects in vitro.Compared with the water group,SJZT and SLBZS were found to possess signif icant gastric acid neutralizing effects.The duration for consistent neutralization of SLBZS was signif icantly longer than that of water.Also,SLBZS and SJZT exhibited signif icant antacid capacities compared to water.CONCLUSION:SJZT and SLBZS were consistently active in the artificial stomach model and are suggested to have antacid effects similar to the active control drugs.
基金supported by the Developmental Fund of Chen Keji Integrative Medicine,No.CKJ2010025the Key Foundation of Society Development in Fujian Province,No.2013Y0059
文摘Wistar rats were intragastrical y perfused with Chinese medicines used for tonifying the kidney. These included 0.180 g/mL of Herba Epimedi （Epimedium）, Semen Cuscutae （Dodder Seed）, or Herba Cistanches （Desertliving Cistanche）, 0.04 mg/mL monoamine oxidase-B inhibitor selegiline, or distil ed water for 14 consecutive days to prepare drug-containing serum or blank serum. MES23.5 cells in the logarithmic phase were cultured in media supplemented with 15%drug-containing serum for 24 hours, fol owed by incubation in culture solution containing 100μmol/L H2O2 for 3 hours. 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） and flow tometry results showed that al drug-containing serums improved the survival rate of H 2 O 2-injured MES23.5 cells, inhibited pro-apoptotic FasL and caspase-3 expression, promoted anti-apoptotic Bcl-2 expression. However, drug-containing serums had little influence on Fas expression in H 2 O 2-injured MES23.5 cells. Enzyme-linked immunosorbent assay results showed that serum containing Herba Cistanches or Herba Epimedi increased the expression of nerve growth factor, brain-derived neurotrophic factor, and glial cellline-derived neurotrophic factor in injured MES23.5 cells;serum containing Semen Cuscutae only increased brain-derived neurotrophic factor expres-sion; while expression of the above neurotrophic factors remained the same in cells treated with serum containing selegiline. These findings indicate that Chinese medicines used to tonify the kid-ney can protect nerve cells by regulating the expression of apoptosis-related factors and neuro-trophic factors in MES23.5 cells.
基金Henan Provincial Science and Technology Research and Development Project(232301420018)Henan Provincial Medical Science and Technology Research Project(LHGJ20230715)Henan Provincial Natural Science Foundation Project(202300410249).
文摘Objective Our objective was to screen drugs with good protective effects on gefitinib-induced hepatotoxicity.Methods Fifty-four specific pathogen-free-grade male mice of the Institute of Cancer Research were randomly divided into a normal group,gefitinib group,glutathione group,ligustrazine group,silymarin group,glycyrrhizic acid group,baicalin group,paeoniflorin group,and matrine group,with six mice in each group.Except for the normal group,the remaining groups of mice were intragastrically administered 400 mg·kg^(-1)of gefitinib for 16 days to induce liver injury.Mice in each treatment group were intragastrically administered 100 mg·kg^(-1)of the corresponding drug 30 minutes after gefitinib administration each day.The normal group and model group mice were intragastrically administered with an equal volume of 0.5%carboxy-methylcellulose sodium(CMC-Na),and the administration volume was 10 mg·kg^(-1).Then,30 minutes after the last administration,blood was collected from the retro-orbital venous plexus,and the mice were killed by cervical dislocation to obtain liver weight and calculate the liver index.Liver pathological changes were observed by hematoxylin–eosin(HE)staining;the levels of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in serum were detected using biochemical kits.AML12 cells were cultured in a medium containing drugs for 30 minutes.Except for the normal group,the remaining groups were induced cell damage with 20μmoL·L1 gefitinib for 24 hours.Cell viability was detected using a cell counting kit-8,and the levels of ALT,AST,and lactate dehydrogenase(LDH)in the cell culture supernatant were measured using biochemical kits.Results Animal experiments showed that compared with the gefitinib group,glycyr-rhizic acid and baicalin significantly increased the body weight of mice(p<0.01)and decreased the liver index and levels of ALT and AST(p<0.05);ligustrazine and silymarin significantly increased the body weight of mice(p<0.01)and decreased the level of AST(p<0.05);paeoniflorin and matrine significantly decreased the levels of ALT and AST(p<0.01).HE staining showed that the liver tissue of mice in the gefitinib group presented a large number of inflammatory cell infiltrations and disordered arrange-ment of hepatic cords;glutathione,glycyrrhizic acid,baicalin,paeoniflorin,and matrine significantly alleviated pathological damage to mouse liver tissue.Cell experi-ments showed that all drugs could alleviate gefitinib-induced damage to AML12 cells to varying degrees,among which glycyrrhizic acid,baicalin,and paeoniflorin had better protective effects,with cell survival rates increased to 96.4,81.1,and 78.2%,respectively.Compared with the gefitinib group,glycyrrhizic acid significantly reduced the levels of ALT,AST,and LDH(p<0.05);silymarin,baicalin,and paeoniflorin significantly reduced the levels of ALT and LDH(p<0.05).Conclusion Glycyrrhizic acid,baicalin,and paeoniflorin all have good protective effects against gefitinib-induced hepatotoxicity,among which glycyrrhizic acid has the best effect.
