Effects of acupuncture combined with Kaijingtongmai Decoction on ATP sensitive potassium channel related proteins Kir6.1 and Kir6.2 in myocardial infarction rats

Objective:To study the effect of combination of acupuncture and medicine on the expression of ATP sensitive potassium channel related proteins Kir6.1 and Kir6.2 in rats with myocardial infarction,and to study the possible mechanism of combination of acupuncture and medicine on the improvement of myocardial infarction,so as to provide experimental data basis for the development of new treatment methods for myocardial infarction.Methods:65 healthy male SD rats were randomly selected as the control group.The other rats were fed with high-fat food for three weeks.The rats in the control group were injected with normal saline subcutaneously,and the other rats were injected with isoproterenol hydrochloride in the same way.Through ECG comparison,40 successful Mi rats were randomly divided into model group,acupuncture group,western medicine group and acupuncture drug combination group,with 10 rats in each group.After the corresponding treatment,the ECG changes of rats in each group were observed,the pathological changes of rat cardiomyocytes were observed by HE staining,and the expression of ATP sensitive potassium channel(Kir6.1,Kir6.2)protein was detected by Western blot.Results:compared with the control group,40 experimental specimens in the experimental group showed significant changes in cardiomyocyte protein The expression of Kir6.1 and Kir6.2 increased,and the difference was statistically significant.After treatment,compared with the model group,the protein expression of Kir6.1 and Kir6.2 in cardiomyocytes of Western medicine group,acupuncture group and acupuncture drug combination group showed a downward trend,among which the decline degree of acupuncture drug combination group was the most obvious,and the difference was statistically significant.The decline degree of acupuncture group and Western medicine group was not significant,and there was no significant difference Conclusion:acupuncture combined with medicine has a significant effect on improving myocardial infarction in rats,which may be related to the expression of ATP sensitive potassium channel related proteins Kir6.1 and Kir6.2 in rat cardiomyocytes.

Mechanism of traditional Chinese medicine in the treatment of allergic rhinitis

Objective To review the major progress in mechanism of traditional Chinese medicine （TCM） in the treatment of allergic rhinitis （AR）. Methods Contents about the treatment mechanism of TCM in the therapy of AR in this article were obtained from 22 original articles and reviews published in Chinese- and English-language journals. All of the references were searched by use of Pubmed （1997-2012）. Results AR is one of the most common and most serious public health problems in children and young people. Many AR patients were worried about the possible adverse effects of synthetic drugs they were taking. Thus, they seek complementary and alternative therapy, such as TCM. TCM emphasized on the importance of holistic convalescence, not just the disease itself. The favorable safety profile of TCM makes well-acceptance by the general population. In the recent decade, more and more studies of TCM for AR are developed. These studies indicated that the treatment of allergic disorders with TCM therapy including herbal medicines and acupuncture are of safety and efficacy. The mechanism of TCM in the treatment of AR has been discussed. It has been reported that a number of the herbs in the Chinese herbal formulae used in the treatment possess anti-allergic, anti-inflammatory or immune modulation activity. Such function include the inhibition of the release or the activity of mast cell mediators （such as histamine）, inhibition the induction of inflammation reaction by chemical agents, and down regulation of serum （immunoglobulin E） IgE levels or the activity of lymphocyte and/or macrophage. Conclusions TCM are frequently used concurrently to improve the clinical efficacy. This review is focuses on the description of the actions mechanism of Chinese medicine＇s approach relevant to the treatment of AR.