Objective:To explore the structural and functional characteristics of microbiota in oropharynx of subhealthy children with gastrointestinal heat retention syndrome(GHRS)differentiated by traditional Chinese medicine,a...Objective:To explore the structural and functional characteristics of microbiota in oropharynx of subhealthy children with gastrointestinal heat retention syndrome(GHRS)differentiated by traditional Chinese medicine,and screen the biometric operational taxonomic units(OTUs)to assist the clinical diagnosis.Methods:We recruited children according to the“GHRS diagnostic scale”,collected their oropharyngeal swabs,and sequenced the 16 SrDNA V4 region.We described the bacterial structure with alpha-indexes,beta-distances,and relative abundances;moreover,we screened the differential genera/OTUs with Wilcoxon rank-sum test,Metagenome Seq analysis,and linear discriminant analysis effect size(LEf Se)analysis,in which biometric OTUs were selected to construct the receiver operating characteristic curve to verify the diagnostic value.The bacterial function was predicted with Kyoto Encyclopedia of Genes and Genomes pathways according to 16S rDNA gene by using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States.Results:The study population was composed of 10 children with GHRS and 10 healthy control children.GHRS children were more likely to overeat(gluttony,P=.033).Alpha-indexes,such as Sobs,abundancebased coverage estimator,Bootstrap,and Qstat,were significantly higher in the GHRS group,while betadistances did not exhibit any significant intergroup differences.There were 9 differently distributed nonpredominant genera between the groups in Wilcoxon rank-sum test,as well as 13 non-predominant genera in Metagenome Seq analysis and 3 non-predominant OTUs in LEfSe analysis.OTU44 and OTU196 were used to construct the receiver operating characteristic curve,and the area under curve was 0.92.Predicted functions showed that pathways related to oxidative phosphorylation and carbon metabolism were enriched in healthy control samples,while the pathway related to renin secretion was remarkably enriched in GHRS samples.Conclusion:Unique oropharyngeal microbial structure and function were identified in GHRS children.OTU44 and OTU196 were specific OTUs,which could be used as biomarkers of GHRS to assist clinical diagnosis.展开更多
Games and songs can bring a lot of pleasure to children. They are useful tools in children's language learning. As English teachers, we should know how to make full use of them to stimulate children's interest...Games and songs can bring a lot of pleasure to children. They are useful tools in children's language learning. As English teachers, we should know how to make full use of them to stimulate children's interest and promote their learning. In this essay, I will take one primary English lesson as an example to demonstrate my point. First I am going to talk about the advantages of using a game and a song, and then analyze the teaching or learning context and the activities in the lesson. At last I am going to focus on how and why to use the game and the song in the classroom. In a word, I am going to justify my choice and use of a game and a song in a revision lesson.展开更多
OBJECTIVE:To develop Clinical practice s of Traditional Chinese Medicine(TCM) for acute upper respiratory tract infection(AURI) in children;TCM is usedalone or administered together with antibiotics.METHODS:Under the ...OBJECTIVE:To develop Clinical practice s of Traditional Chinese Medicine(TCM) for acute upper respiratory tract infection(AURI) in children;TCM is usedalone or administered together with antibiotics.METHODS:Under the guidance of evidence-based medicine concept,in strict accordance with the rules of international s development,as well as on the basis of evidence of clinical research of TCM,the s solicited opinions from clinical experts and methodologists in TCM and Western Medicine.GRADE standard was applied to form experts' consensus.RESULTS:The s standardized classification of TCM patterns and TCM treatments in children with AURI,including prescription,Chinese patent medicine,non-drug treatment and prevention.CONCLUSION:Follows the principle of "evidence based,consensus supplemented,and experience referred",these s were formulated,but the quality of evidence of included studies were relatively low.Further refinement of the s should be needed as deeper clinical studies as available in future.展开更多
Purpose-The purpose of this paper is to explore the experience of the weight of a backpack as represented in typical Chinese children’s songs through a kind of phenomenological approach.Design/Approach/Methods-The co...Purpose-The purpose of this paper is to explore the experience of the weight of a backpack as represented in typical Chinese children’s songs through a kind of phenomenological approach.Design/Approach/Methods-The core issue pertaining to Chinese children’s songs about backpacks is how the weight sense of backpacks is generated and developed in the field of song phenomenology.Findings-The“whatness”of a backpack in typical songs has varied over the past six decades,meanwhile,the backpack and its songs conceal not only the secrets of childhood but also those of China’s educational system.Originality/Value-The originality of this paper embodies the unique perspective to provide a new insight into the current scholarship of the micro-politics of song-singing and the weight sense of backpack.展开更多
In 1990, the Children’s and Women’s Activity Center of Hunan Province established a study office for children’s paintings. Ever since then, the center has offered painting courses for children in the province. The ...In 1990, the Children’s and Women’s Activity Center of Hunan Province established a study office for children’s paintings. Ever since then, the center has offered painting courses for children in the province. The teacher is展开更多
基金supported by the National Natural Science Foundation of China(81373769)the Scientific Research and Postgraduate Training Co-construction Project of Beijing Municipal Education Commission(1000062520115)the Province Natural Science Foundation of Beijing(7172131)。
文摘Objective:To explore the structural and functional characteristics of microbiota in oropharynx of subhealthy children with gastrointestinal heat retention syndrome(GHRS)differentiated by traditional Chinese medicine,and screen the biometric operational taxonomic units(OTUs)to assist the clinical diagnosis.Methods:We recruited children according to the“GHRS diagnostic scale”,collected their oropharyngeal swabs,and sequenced the 16 SrDNA V4 region.We described the bacterial structure with alpha-indexes,beta-distances,and relative abundances;moreover,we screened the differential genera/OTUs with Wilcoxon rank-sum test,Metagenome Seq analysis,and linear discriminant analysis effect size(LEf Se)analysis,in which biometric OTUs were selected to construct the receiver operating characteristic curve to verify the diagnostic value.The bacterial function was predicted with Kyoto Encyclopedia of Genes and Genomes pathways according to 16S rDNA gene by using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States.Results:The study population was composed of 10 children with GHRS and 10 healthy control children.GHRS children were more likely to overeat(gluttony,P=.033).Alpha-indexes,such as Sobs,abundancebased coverage estimator,Bootstrap,and Qstat,were significantly higher in the GHRS group,while betadistances did not exhibit any significant intergroup differences.There were 9 differently distributed nonpredominant genera between the groups in Wilcoxon rank-sum test,as well as 13 non-predominant genera in Metagenome Seq analysis and 3 non-predominant OTUs in LEfSe analysis.OTU44 and OTU196 were used to construct the receiver operating characteristic curve,and the area under curve was 0.92.Predicted functions showed that pathways related to oxidative phosphorylation and carbon metabolism were enriched in healthy control samples,while the pathway related to renin secretion was remarkably enriched in GHRS samples.Conclusion:Unique oropharyngeal microbial structure and function were identified in GHRS children.OTU44 and OTU196 were specific OTUs,which could be used as biomarkers of GHRS to assist clinical diagnosis.
文摘Games and songs can bring a lot of pleasure to children. They are useful tools in children's language learning. As English teachers, we should know how to make full use of them to stimulate children's interest and promote their learning. In this essay, I will take one primary English lesson as an example to demonstrate my point. First I am going to talk about the advantages of using a game and a song, and then analyze the teaching or learning context and the activities in the lesson. At last I am going to focus on how and why to use the game and the song in the classroom. In a word, I am going to justify my choice and use of a game and a song in a revision lesson.
文摘OBJECTIVE:To develop Clinical practice s of Traditional Chinese Medicine(TCM) for acute upper respiratory tract infection(AURI) in children;TCM is usedalone or administered together with antibiotics.METHODS:Under the guidance of evidence-based medicine concept,in strict accordance with the rules of international s development,as well as on the basis of evidence of clinical research of TCM,the s solicited opinions from clinical experts and methodologists in TCM and Western Medicine.GRADE standard was applied to form experts' consensus.RESULTS:The s standardized classification of TCM patterns and TCM treatments in children with AURI,including prescription,Chinese patent medicine,non-drug treatment and prevention.CONCLUSION:Follows the principle of "evidence based,consensus supplemented,and experience referred",these s were formulated,but the quality of evidence of included studies were relatively low.Further refinement of the s should be needed as deeper clinical studies as available in future.
文摘Purpose-The purpose of this paper is to explore the experience of the weight of a backpack as represented in typical Chinese children’s songs through a kind of phenomenological approach.Design/Approach/Methods-The core issue pertaining to Chinese children’s songs about backpacks is how the weight sense of backpacks is generated and developed in the field of song phenomenology.Findings-The“whatness”of a backpack in typical songs has varied over the past six decades,meanwhile,the backpack and its songs conceal not only the secrets of childhood but also those of China’s educational system.Originality/Value-The originality of this paper embodies the unique perspective to provide a new insight into the current scholarship of the micro-politics of song-singing and the weight sense of backpack.
文摘In 1990, the Children’s and Women’s Activity Center of Hunan Province established a study office for children’s paintings. Ever since then, the center has offered painting courses for children in the province. The teacher is
