As important geological data,a geological report contains rich expert and geological knowledge,but the challenge facing current research into geological knowledge extraction and mining is how to render accurate unders...As important geological data,a geological report contains rich expert and geological knowledge,but the challenge facing current research into geological knowledge extraction and mining is how to render accurate understanding of geological reports guided by domain knowledge.While generic named entity recognition models/tools can be utilized for the processing of geoscience reports/documents,their effectiveness is hampered by a dearth of domain-specific knowledge,which in turn leads to a pronounced decline in recognition accuracy.This study summarizes six types of typical geological entities,with reference to the ontological system of geological domains and builds a high quality corpus for the task of geological named entity recognition(GNER).In addition,Geo Wo BERT-adv BGP(Geological Word-base BERTadversarial training Bi-directional Long Short-Term Memory Global Pointer)is proposed to address the issues of ambiguity,diversity and nested entities for the geological entities.The model first uses the fine-tuned word granularitybased pre-training model Geo Wo BERT(Geological Word-base BERT)and combines the text features that are extracted using the Bi LSTM(Bi-directional Long Short-Term Memory),followed by an adversarial training algorithm to improve the robustness of the model and enhance its resistance to interference,the decoding finally being performed using a global association pointer algorithm.The experimental results show that the proposed model for the constructed dataset achieves high performance and is capable of mining the rich geological information.展开更多
The purpose of this study is to comprehensively evaluate the modern training model of rehabilitation therapy technology talents.Selecting the third-year students of the rehabilitation therapy technology program in Sch...The purpose of this study is to comprehensively evaluate the modern training model of rehabilitation therapy technology talents.Selecting the third-year students of the rehabilitation therapy technology program in School Y as the research subject,300 questionnaires were collected and the effective response rate was 92%.The strengths and weaknesses of the modern training model were analyzed through a mixed qualitative and quantitative research method.It was found that 68%of the students thought that the modern model had obvious advantages in practical teaching,but 42%of the students thought that it still needed to be improved in personalized teaching.This study provides an empirical basis and specific suggestions for optimizing the cultivation of rehabilitation therapy technology talents.展开更多
Objective:To explore and analyze the work process-based practical training teaching model for basic nursing skills in vocational colleges and its implementation effects.Methods:A total of 82 nursing students from our ...Objective:To explore and analyze the work process-based practical training teaching model for basic nursing skills in vocational colleges and its implementation effects.Methods:A total of 82 nursing students from our school were selected for the study,which was conducted from April 2023 to April 2024.Using a random number table method,the students were divided into an observation group and a control group,each with 41 students.The control group received conventional practical training teaching,while the observation group followed the work process-based practical training model for basic nursing skills.The assessment scores and teaching satisfaction of the two groups were compared.Results:The comparison of assessment scores showed that the observation group performed significantly better than the control group(P<0.05).The comparison of teaching satisfaction also indicated that the observation group had significantly higher satisfaction than the control group(P<0.05).Conclusion:The work process-based practical training teaching model for basic nursing skills in vocational colleges can improve students’assessment scores and enhance teaching satisfaction,demonstrating its value for wider application.展开更多
Leveraging the Baidu Qianfan model platform,this paper designs and implements a highly efficient and accurate scoring system for subjective questions,focusing primarily on questions in the field of computer network te...Leveraging the Baidu Qianfan model platform,this paper designs and implements a highly efficient and accurate scoring system for subjective questions,focusing primarily on questions in the field of computer network technology.The system enhances the foundational model by utilizing Qianfan’s training tools and integrating advanced techniques,such as supervised fine-tuning.In the data preparation phase,a comprehensive collection of subjective data related to computer network technology is gathered,cleaned,and labeled.During model training and evaluation,optimal hyperparameters and tuning strategies are applied,resulting in a model capable of scoring with high accuracy.Evaluation results demonstrate that the proposed model performs well across multiple dimensions-content,expression,and development scores-yielding results comparable to those of manual scoring.展开更多
This study proposes the use of the MERISE conceptual data model to create indicators for monitoring and evaluating the effectiveness of vocational training in the Republic of Congo. The importance of MERISE for struct...This study proposes the use of the MERISE conceptual data model to create indicators for monitoring and evaluating the effectiveness of vocational training in the Republic of Congo. The importance of MERISE for structuring and analyzing data is underlined, as it enables the measurement of the adequacy between training and the needs of the labor market. The innovation of the study lies in the adaptation of the MERISE model to the local context, the development of innovative indicators, and the integration of a participatory approach including all relevant stakeholders. Contextual adaptation and local innovation: The study suggests adapting MERISE to the specific context of the Republic of Congo, considering the local particularities of the labor market. Development of innovative indicators and new measurement tools: It proposes creating indicators to assess skills matching and employer satisfaction, which are crucial for evaluating the effectiveness of vocational training. Participatory approach and inclusion of stakeholders: The study emphasizes actively involving training centers, employers, and recruitment agencies in the evaluation process. This participatory approach ensures that the perspectives of all stakeholders are considered, leading to more relevant and practical outcomes. Using the MERISE model allows for: • Rigorous data structuring, organization, and standardization: Clearly defining entities and relationships facilitates data organization and standardization, crucial for effective data analysis. • Facilitation of monitoring, analysis, and relevant indicators: Developing both quantitative and qualitative indicators helps measure the effectiveness of training in relation to the labor market, allowing for a comprehensive evaluation. • Improved communication and common language: By providing a common language for different stakeholders, MERISE enhances communication and collaboration, ensuring that all parties have a shared understanding. The study’s approach and contribution to existing research lie in: • Structured theoretical and practical framework and holistic approach: The study offers a structured framework for data collection and analysis, covering both quantitative and qualitative aspects, thus providing a comprehensive view of the training system. • Reproducible methodology and international comparison: The proposed methodology can be replicated in other contexts, facilitating international comparison and the adoption of best practices. • Extension of knowledge and new perspective: By integrating a participatory approach and developing indicators adapted to local needs, the study extends existing research and offers new perspectives on vocational training evaluation.展开更多
With the acceleration of the social information process,information awareness and information skills have become the basic qualities of every citizen.The establishment of the training mechanism for scientific and tech...With the acceleration of the social information process,information awareness and information skills have become the basic qualities of every citizen.The establishment of the training mechanism for scientific and technological innovation talents from the beginning of higher education is insufficient to meet the needs of the development of the times.It is imperative to improve the training of information technology innovation talents and explore a new training model.This paper describes the general situation of the development of education in the field of information technology from a domestic and international perspective.It then analyzes the existing problems,explores new exploration models and implementation suggestions,and puts forward prospects at the end of the paper.展开更多
Latent variable models can effectively determine the condition of essential rotating machinery without needing labeled data.These models analyze vibration data via an unsupervised learning strategy.Temporal preservati...Latent variable models can effectively determine the condition of essential rotating machinery without needing labeled data.These models analyze vibration data via an unsupervised learning strategy.Temporal preservation is necessary to obtain an informative latent manifold for the fault diagnosis task.In a temporalpreserving context,two approaches exist to develop a condition-monitoring methodology:offline and online.For latent variable models,the available training modes are not different.While many traditional methods use offline training,online training can dynamically adjust the latent manifold,possibly leading to better fault signature extraction from the vibration data.This study explores online training using temporal-preserving latent variable models.Within online training,there are two main methods:one focuses on reconstructing data and the other on interpreting the data components.Both are considered to evaluate how they diagnose faults over time.Using two experimental datasets,the study confirms that models from both training modes can detect changes in machinery health and identify faults even under varying conditions.Importantly,the complementarity of offline and online models is emphasized,reassuring their versatility in fault diagnostics.Understanding the implications of the training approach and the available model formulations is crucial for further research in latent variable modelbased fault diagnostics.展开更多
Objective:To explore the effectiveness of the competency model for nursing managers in the training of newly appointed head nurses.Methods:Eighteen newly appointed head nurses from Yichang Central People’s Hospital,s...Objective:To explore the effectiveness of the competency model for nursing managers in the training of newly appointed head nurses.Methods:Eighteen newly appointed head nurses from Yichang Central People’s Hospital,selected between August 2023 and July 2024,were chosen as the research subjects.Based on the nursing manager competency model,corresponding training programs were developed,and these 18 head nurses were trained accordingly.Results:After the training,all newly appointed head nurses passed the theoretical assessments,with a pass rate of 100.00%.The pass rates for two rounds of assessment were 100.00%and 94.54%,respectively.There was no significant difference in the performance evaluation scores between new and senior head nurses in terms of standardized services,nursing quality,nursing research and teaching,and patient satisfaction(P>0.05).Conclusion:The competency model for nursing managers has a significant effect on the training of newly appointed head nurses.It effectively enhances management awareness,facilitates role transition,and,to some extent,improves the management and leadership capabilities of head nurses.It holds high value for broader implementation.展开更多
By analyzing the complementarity between smart agriculture and farming-reading education,and the advantages of organic combination of the two:addressing the challenges of agricultural modernization and cultivating new...By analyzing the complementarity between smart agriculture and farming-reading education,and the advantages of organic combination of the two:addressing the challenges of agricultural modernization and cultivating new talents;enhancing students interest in learning and improving their sense of responsibility and mission;promoting the inheritance of traditional agricultural culture and modern agricultural technology innovation,the organic combination mode of smart agriculture and farming-reading education in the cultivation of new agricultural talents is explored.The analysis shows that it has the following applications in the cultivation of new agricultural talents:in terms of the integration of educational concepts,it combines the modern concept of smart agriculture with the traditional value of farming-reading education;in terms of constructing a curriculum system and adding courses related to smart agriculture,it retains basic courses in farming-reading education,and promotes interdisciplinary integration;in terms of practical teaching platforms,it establishes smart agriculture training bases and farming-reading education bases,and adopts a combination of virtual and real teaching methods;in terms of guiding innovation and entrepreneurship,it encourages students to participate in innovation and entrepreneurship projects,and strengthens industry-university-research cooperation;in terms of teacher team construction,it improves the quality of the teacher team and integrates educational resources both inside and outside the school.Moreover,its evaluation system and incentive mechanism are proposed:constructing a multi-dimensional evaluation system,combining quantitative and qualitative,process and result evaluation,and introducing the third-party evaluation;establishing scholarships and grants,providing support for innovation and entrepreneurship,conducting honor recognition and publicity,and offering career development support.展开更多
The recent increase in the use of artificial intelligence has led to fundamental changes in the development of training and teaching methods for executive education. However, the success of artificial intelligence in ...The recent increase in the use of artificial intelligence has led to fundamental changes in the development of training and teaching methods for executive education. However, the success of artificial intelligence in regional centers for teaching and training professions will depend on the acceptance of this technology by young executive trainees. This article discusses the potential benefits of adopting AI in executive training institutions in Morocco, specifically focusing on CRMEF Casablanca Settat. Based on the Unified Theory of Acceptance and Use of Technology (UTAUT) (Venkatesh et al., 2003), this study proposes a model to identify the factors influencing the acceptance of artificial intelligence in regional centers for teaching professions and training in Morocco. To achieve this, a structural equation modeling approach was used to quantitatively describe the impact of each factor on AI adoption, utilizing data collected from 173 young executive trainees. The results indicate that perceived ease of use, perceived usefulness, trainer influence, and personal innovativeness influence the intention to use artificial intelligence. Our research provides managers of CRMEFs with a set of practical recommendations to enhance the implementation conditions of an artificial intelligence system. It aims to understand which factors should be considered in designing an artificial intelligence system within regional centers for teaching professions and training (CRMEFs).展开更多
At present,as China vigorously develops new energy automobiles,the social demand for related talents has increased significantly,so the relevant institutions need to optimize and adjust the talent training mode to hel...At present,as China vigorously develops new energy automobiles,the social demand for related talents has increased significantly,so the relevant institutions need to optimize and adjust the talent training mode to help the development of new energy automobiles.This paper summarizes the existing problems of China’s new energy automobile talent training mode,and analyzes the significance of the virtual simulation-based dual-creation talent training mode of new energy automobile and its specific implementation strategies,in order to provide references for the relevant personnel.展开更多
Against the backdrop of global climate change and China’s“dual carbon”goals,the green transformation of the construction industry is imperative,and completing the transformation requires many dual carbon talents to...Against the backdrop of global climate change and China’s“dual carbon”goals,the green transformation of the construction industry is imperative,and completing the transformation requires many dual carbon talents to support it.This article focuses on the construction engineering technology major in higher vocational education.It explores in depth the specific requirements for construction engineering technology talents in terms of professional knowledge,vocational skills,and literacy under the dual carbon mode.Based on this,corresponding dual carbon talent training courses are proposed,aiming to provide theoretical support and practical guidance for cultivating high-quality dual carbon talents that meet the needs of the new era.展开更多
The objective of this study is to examine the application effect of the Objective Structured Clinical Examination(OSCE)model in the comprehensive practical training course for higher vocational nursing.Based on the ev...The objective of this study is to examine the application effect of the Objective Structured Clinical Examination(OSCE)model in the comprehensive practical training course for higher vocational nursing.Based on the evaluation dimensions of core nursing competencies,the OSCE framework was constructed with the clinical nursing work process as the main focus and supported by a nursing case database.A questionnaire survey revealed that over 80%of students in the experimental group were satisfied with the application of OSCE in the nursing comprehensive practical training course,and more than 90%believed that OSCE assessment enhanced their theoretical knowledge and practical skills.The OSCE was also considered helpful in transforming learned knowledge and skills into professional competencies.展开更多
The current situation of European educational systems leads to the lack of historical precedents,considering that it implies the end of educational systems based on exclusion,configuring a new conception of educationa...The current situation of European educational systems leads to the lack of historical precedents,considering that it implies the end of educational systems based on exclusion,configuring a new conception of educational systems that we are still not able to appreciate at their true value,because we were educated by and in the previous educational system.Lacking other references,we inevitably tend to compare current problems with previous situations,without understanding that the generalization of education to 100%of the population implies a qualitative change that modifies the objectives,forms of work,and the very essence of the educational system.This stage change,marked by the acceleration of social changes,including the pandemic situation,explains why,despite the best educational system so far,teachers with the best levels of training and a financial and material endowment unimaginable until recently,try a sense of crisis and a general feeling of confusion is manifested among the teachers and parents of the children,seeing that education is no longer what it used to be(Esteve,1998).The key to defuse this apparent paradox is in the tendency to judge the new education system by the mentality and standards of the former selective education,in which most of the adults present were educated.Thus,although we continue to apply to the new general education situation the parameters of the previous situation,in which the selective system excluded every year the students who had behavior problems in the class or had difficulties regarding the mastery of certain educational subjects(Esteve,1998).In this way,we are currently criticizing the school failure of a percentage of our students,without realizing that their schooling itself is a success,because,although their level is low,they had no level before.One speaks of a low educational level,when,in reality,the educational level is increasing—the general level of the people and the individual level of the best students(Baudelot&Establet,1990).Indeed,it is true that in our education systems there are students with a very low educational level,although even so,it is a success that they have some education because they used to be on the streets;and,in any case,the current education system produces a higher percentage of students with higher levels than the best students of the 90s.The main element of change,the most substantial transformation,is the elimination of exclusion,today having access to secondary education,alongside students with an excellent level.Thousands of children who were previously expelled from it,thus comply with the European measures of access to education for all children and young people.The previous educational system can be compared to a hospital from which the sickest were kicked out,and those who created behavioral problems were eliminated through the selective mechanisms of the system.However,teaching today is qualitatively different from what it was 20-30 years ago.First of all,because working with a homogeneous group of children as a result of the selection or dealing with all the children in a country,with social and personal problems,is not the same thing.That is why many general education teachers are social workers rather than teachers;and the new configuration of our educational systems requires secondary teachers to assume diverse educational roles rather than the traditional one of intellectual training.History cannot go backwards;to have better quality educational systems,the solution is not to return to the exclusionary system.Society asks teachers to make an effort to integrate;but,during this time,our society must support and revalue the activity of teachers in order not to put them in front of impossible tasks.The transition from a selective to a general education was based on the increase/amplification of compulsory schooling.For the first time in history,European educational systems have assumed the role of declaring secondary education compulsory.This decision implies a new stage,a definitive break from the previous reality in which secondary education was reserved for those who later attend a university.展开更多
Neuromyelitis optica spectrum disorders are neuroinflammatory demyelinating disorders that lead to permanent visual loss and motor dysfunction.To date,no effective treatment exists as the exact causative mechanism rem...Neuromyelitis optica spectrum disorders are neuroinflammatory demyelinating disorders that lead to permanent visual loss and motor dysfunction.To date,no effective treatment exists as the exact causative mechanism remains unknown.Therefore,experimental models of neuromyelitis optica spectrum disorders are essential for exploring its pathogenesis and in screening for therapeutic targets.Since most patients with neuromyelitis optica spectrum disorders are seropositive for IgG autoantibodies against aquaporin-4,which is highly expressed on the membrane of astrocyte endfeet,most current experimental models are based on aquaporin-4-IgG that initially targets astrocytes.These experimental models have successfully simulated many pathological features of neuromyelitis optica spectrum disorders,such as aquaporin-4 loss,astrocytopathy,granulocyte and macrophage infiltration,complement activation,demyelination,and neuronal loss;however,they do not fully capture the pathological process of human neuromyelitis optica spectrum disorders.In this review,we summarize the currently known pathogenic mechanisms and the development of associated experimental models in vitro,ex vivo,and in vivo for neuromyelitis optica spectrum disorders,suggest potential pathogenic mechanisms for further investigation,and provide guidance on experimental model choices.In addition,this review summarizes the latest information on pathologies and therapies for neuromyelitis optica spectrum disorders based on experimental models of aquaporin-4-IgG-seropositive neuromyelitis optica spectrum disorders,offering further therapeutic targets and a theoretical basis for clinical trials.展开更多
This study was aimed to prepare landslide susceptibility maps for the Pithoragarh district in Uttarakhand,India,using advanced ensemble models that combined Radial Basis Function Networks(RBFN)with three ensemble lear...This study was aimed to prepare landslide susceptibility maps for the Pithoragarh district in Uttarakhand,India,using advanced ensemble models that combined Radial Basis Function Networks(RBFN)with three ensemble learning techniques:DAGGING(DG),MULTIBOOST(MB),and ADABOOST(AB).This combination resulted in three distinct ensemble models:DG-RBFN,MB-RBFN,and AB-RBFN.Additionally,a traditional weighted method,Information Value(IV),and a benchmark machine learning(ML)model,Multilayer Perceptron Neural Network(MLP),were employed for comparison and validation.The models were developed using ten landslide conditioning factors,which included slope,aspect,elevation,curvature,land cover,geomorphology,overburden depth,lithology,distance to rivers and distance to roads.These factors were instrumental in predicting the output variable,which was the probability of landslide occurrence.Statistical analysis of the models’performance indicated that the DG-RBFN model,with an Area Under ROC Curve(AUC)of 0.931,outperformed the other models.The AB-RBFN model achieved an AUC of 0.929,the MB-RBFN model had an AUC of 0.913,and the MLP model recorded an AUC of 0.926.These results suggest that the advanced ensemble ML model DG-RBFN was more accurate than traditional statistical model,single MLP model,and other ensemble models in preparing trustworthy landslide susceptibility maps,thereby enhancing land use planning and decision-making.展开更多
This study directs the discussion of HIV disease with a novel kind of complex dynamical generalized and piecewise operator in the sense of classical and Atangana Baleanu(AB)derivatives having arbitrary order.The HIV i...This study directs the discussion of HIV disease with a novel kind of complex dynamical generalized and piecewise operator in the sense of classical and Atangana Baleanu(AB)derivatives having arbitrary order.The HIV infection model has a susceptible class,a recovered class,along with a case of infection divided into three sub-different levels or categories and the recovered class.The total time interval is converted into two,which are further investigated for ordinary and fractional order operators of the AB derivative,respectively.The proposed model is tested separately for unique solutions and existence on bi intervals.The numerical solution of the proposed model is treated by the piece-wise numerical iterative scheme of Newtons Polynomial.The proposed method is established for piece-wise derivatives under natural order and non-singular Mittag-Leffler Law.The cross-over or bending characteristics in the dynamical system of HIV are easily examined by the aspect of this research having a memory effect for controlling the said disease.This study uses the neural network(NN)technique to obtain a better set of weights with low residual errors,and the epochs number is considered 1000.The obtained figures represent the approximate solution and absolute error which are tested with NN to train the data accurately.展开更多
BACKGROUND Rebleeding after recovery from esophagogastric variceal bleeding(EGVB)is a severe complication that is associated with high rates of both incidence and mortality.Despite its clinical importance,recognized p...BACKGROUND Rebleeding after recovery from esophagogastric variceal bleeding(EGVB)is a severe complication that is associated with high rates of both incidence and mortality.Despite its clinical importance,recognized prognostic models that can effectively predict esophagogastric variceal rebleeding in patients with liver cirrhosis are lacking.AIM To construct and externally validate a reliable prognostic model for predicting the occurrence of esophagogastric variceal rebleeding.METHODS This study included 477 EGVB patients across 2 cohorts:The derivation cohort(n=322)and the validation cohort(n=155).The primary outcome was rebleeding events within 1 year.The least absolute shrinkage and selection operator was applied for predictor selection,and multivariate Cox regression analysis was used to construct the prognostic model.Internal validation was performed with bootstrap resampling.We assessed the discrimination,calibration and accuracy of the model,and performed patient risk stratification.RESULTS Six predictors,including albumin and aspartate aminotransferase concentrations,white blood cell count,and the presence of ascites,portal vein thrombosis,and bleeding signs,were selected for the rebleeding event prediction following endoscopic treatment(REPET)model.In predicting rebleeding within 1 year,the REPET model ex-hibited a concordance index of 0.775 and a Brier score of 0.143 in the derivation cohort,alongside 0.862 and 0.127 in the validation cohort.Furthermore,the REPET model revealed a significant difference in rebleeding rates(P<0.01)between low-risk patients and intermediate-to high-risk patients in both cohorts.CONCLUSION We constructed and validated a new prognostic model for variceal rebleeding with excellent predictive per-formance,which will improve the clinical management of rebleeding in EGVB patients.展开更多
Rare neurological diseases,while individually are rare,collectively impact millions globally,leading to diverse and often severe neurological symptoms.Often attributed to genetic mutations that disrupt protein functio...Rare neurological diseases,while individually are rare,collectively impact millions globally,leading to diverse and often severe neurological symptoms.Often attributed to genetic mutations that disrupt protein function or structure,understanding their genetic basis is crucial for accurate diagnosis and targeted therapies.To investigate the underlying pathogenesis of these conditions,researchers often use non-mammalian model organisms,such as Drosophila(fruit flies),which is valued for their genetic manipulability,cost-efficiency,and preservation of genes and biological functions across evolutionary time.Genetic tools available in Drosophila,including CRISPR-Cas9,offer a means to manipulate gene expression,allowing for a deep exploration of the genetic underpinnings of rare neurological diseases.Drosophila boasts a versatile genetic toolkit,rapid generation turnover,and ease of large-scale experimentation,making it an invaluable resource for identifying potential drug candidates.Researchers can expose flies carrying disease-associated mutations to various compounds,rapidly pinpointing promising therapeutic agents for further investigation in mammalian models and,ultimately,clinical trials.In this comprehensive review,we explore rare neurological diseases where fly research has significantly contributed to our understanding of their genetic basis,pathophysiology,and potential therapeutic implications.We discuss rare diseases associated with both neuron-expressed and glial-expressed genes.Specific cases include mutations in CDK19 resulting in epilepsy and developmental delay,mutations in TIAM1 leading to a neurodevelopmental disorder with seizures and language delay,and mutations in IRF2BPL causing seizures,a neurodevelopmental disorder with regression,loss of speech,and abnormal movements.And we explore mutations in EMC1 related to cerebellar atrophy,visual impairment,psychomotor retardation,and gain-of-function mutations in ACOX1 causing Mitchell syndrome.Loss-of-function mutations in ACOX1 result in ACOX1 deficiency,characterized by very-long-chain fatty acid accumulation and glial degeneration.Notably,this review highlights how modeling these diseases in Drosophila has provided valuable insights into their pathophysiology,offering a platform for the rapid identification of potential therapeutic interventions.Rare neurological diseases involve a wide range of expression systems,and sometimes common phenotypes can be found among different genes that cause abnormalities in neurons or glia.Furthermore,mutations within the same gene may result in varying functional outcomes,such as complete loss of function,partial loss of function,or gain-of-function mutations.The phenotypes observed in patients can differ significantly,underscoring the complexity of these conditions.In conclusion,Drosophila represents an indispensable and cost-effective tool for investigating rare neurological diseases.By facilitating the modeling of these conditions,Drosophila contributes to a deeper understanding of their genetic basis,pathophysiology,and potential therapies.This approach accelerates the discovery of promising drug candidates,ultimately benefiting patients affected by these complex and understudied diseases.展开更多
To investigate the mechanisms underlying the onset and progression of ischemic stroke,some methods have been proposed that can simultaneously monitor and create embolisms in the animal cerebral cortex.However,these me...To investigate the mechanisms underlying the onset and progression of ischemic stroke,some methods have been proposed that can simultaneously monitor and create embolisms in the animal cerebral cortex.However,these methods often require complex systems and the effect of age on cerebral embolism has not been adequately studied,although ischemic stroke is strongly age-related.In this study,we propose an optical-resolution photoacoustic microscopy-based visualized photothrombosis methodology to create and monitor ischemic stroke in mice simultaneously using a 532 nm pulsed laser.We observed the molding process in mice of different ages and presented age-dependent vascular embolism differentiation.Moreover,we integrated optical coherence tomography angiography to investigate age-associated trends in cerebrovascular variability following a stroke.Our imaging data and quantitative analyses underscore the differential cerebrovascular responses to stroke in mice of different ages,thereby highlighting the technique's potential for evaluating cerebrovascular health and unraveling age-related mechanisms involved in ischemic strokes.展开更多
基金financially supported by the Natural Science Foundation of China(Grant No.42301492)the National Key R&D Program of China(Grant Nos.2022YFF0711600,2022YFF0801201,2022YFF0801200)+3 种基金the Major Special Project of Xinjiang(Grant No.2022A03009-3)the Open Fund of Key Laboratory of Urban Land Resources Monitoring and Simulation,Ministry of Natural Resources(Grant No.KF-2022-07014)the Opening Fund of the Key Laboratory of the Geological Survey and Evaluation of the Ministry of Education(Grant No.GLAB 2023ZR01)the Fundamental Research Funds for the Central Universities。
文摘As important geological data,a geological report contains rich expert and geological knowledge,but the challenge facing current research into geological knowledge extraction and mining is how to render accurate understanding of geological reports guided by domain knowledge.While generic named entity recognition models/tools can be utilized for the processing of geoscience reports/documents,their effectiveness is hampered by a dearth of domain-specific knowledge,which in turn leads to a pronounced decline in recognition accuracy.This study summarizes six types of typical geological entities,with reference to the ontological system of geological domains and builds a high quality corpus for the task of geological named entity recognition(GNER).In addition,Geo Wo BERT-adv BGP(Geological Word-base BERTadversarial training Bi-directional Long Short-Term Memory Global Pointer)is proposed to address the issues of ambiguity,diversity and nested entities for the geological entities.The model first uses the fine-tuned word granularitybased pre-training model Geo Wo BERT(Geological Word-base BERT)and combines the text features that are extracted using the Bi LSTM(Bi-directional Long Short-Term Memory),followed by an adversarial training algorithm to improve the robustness of the model and enhance its resistance to interference,the decoding finally being performed using a global association pointer algorithm.The experimental results show that the proposed model for the constructed dataset achieves high performance and is capable of mining the rich geological information.
基金Henan Provincial Medical Education Research Project“Research on the Innovation and Practice of Talent Cultivation Mode of Rehabilitation Therapy Technology Based on the Collaborative Education and Training”(Project number:WJLX2023208)。
文摘The purpose of this study is to comprehensively evaluate the modern training model of rehabilitation therapy technology talents.Selecting the third-year students of the rehabilitation therapy technology program in School Y as the research subject,300 questionnaires were collected and the effective response rate was 92%.The strengths and weaknesses of the modern training model were analyzed through a mixed qualitative and quantitative research method.It was found that 68%of the students thought that the modern model had obvious advantages in practical teaching,but 42%of the students thought that it still needed to be improved in personalized teaching.This study provides an empirical basis and specific suggestions for optimizing the cultivation of rehabilitation therapy technology talents.
文摘Objective:To explore and analyze the work process-based practical training teaching model for basic nursing skills in vocational colleges and its implementation effects.Methods:A total of 82 nursing students from our school were selected for the study,which was conducted from April 2023 to April 2024.Using a random number table method,the students were divided into an observation group and a control group,each with 41 students.The control group received conventional practical training teaching,while the observation group followed the work process-based practical training model for basic nursing skills.The assessment scores and teaching satisfaction of the two groups were compared.Results:The comparison of assessment scores showed that the observation group performed significantly better than the control group(P<0.05).The comparison of teaching satisfaction also indicated that the observation group had significantly higher satisfaction than the control group(P<0.05).Conclusion:The work process-based practical training teaching model for basic nursing skills in vocational colleges can improve students’assessment scores and enhance teaching satisfaction,demonstrating its value for wider application.
文摘Leveraging the Baidu Qianfan model platform,this paper designs and implements a highly efficient and accurate scoring system for subjective questions,focusing primarily on questions in the field of computer network technology.The system enhances the foundational model by utilizing Qianfan’s training tools and integrating advanced techniques,such as supervised fine-tuning.In the data preparation phase,a comprehensive collection of subjective data related to computer network technology is gathered,cleaned,and labeled.During model training and evaluation,optimal hyperparameters and tuning strategies are applied,resulting in a model capable of scoring with high accuracy.Evaluation results demonstrate that the proposed model performs well across multiple dimensions-content,expression,and development scores-yielding results comparable to those of manual scoring.
文摘This study proposes the use of the MERISE conceptual data model to create indicators for monitoring and evaluating the effectiveness of vocational training in the Republic of Congo. The importance of MERISE for structuring and analyzing data is underlined, as it enables the measurement of the adequacy between training and the needs of the labor market. The innovation of the study lies in the adaptation of the MERISE model to the local context, the development of innovative indicators, and the integration of a participatory approach including all relevant stakeholders. Contextual adaptation and local innovation: The study suggests adapting MERISE to the specific context of the Republic of Congo, considering the local particularities of the labor market. Development of innovative indicators and new measurement tools: It proposes creating indicators to assess skills matching and employer satisfaction, which are crucial for evaluating the effectiveness of vocational training. Participatory approach and inclusion of stakeholders: The study emphasizes actively involving training centers, employers, and recruitment agencies in the evaluation process. This participatory approach ensures that the perspectives of all stakeholders are considered, leading to more relevant and practical outcomes. Using the MERISE model allows for: • Rigorous data structuring, organization, and standardization: Clearly defining entities and relationships facilitates data organization and standardization, crucial for effective data analysis. • Facilitation of monitoring, analysis, and relevant indicators: Developing both quantitative and qualitative indicators helps measure the effectiveness of training in relation to the labor market, allowing for a comprehensive evaluation. • Improved communication and common language: By providing a common language for different stakeholders, MERISE enhances communication and collaboration, ensuring that all parties have a shared understanding. The study’s approach and contribution to existing research lie in: • Structured theoretical and practical framework and holistic approach: The study offers a structured framework for data collection and analysis, covering both quantitative and qualitative aspects, thus providing a comprehensive view of the training system. • Reproducible methodology and international comparison: The proposed methodology can be replicated in other contexts, facilitating international comparison and the adoption of best practices. • Extension of knowledge and new perspective: By integrating a participatory approach and developing indicators adapted to local needs, the study extends existing research and offers new perspectives on vocational training evaluation.
文摘With the acceleration of the social information process,information awareness and information skills have become the basic qualities of every citizen.The establishment of the training mechanism for scientific and technological innovation talents from the beginning of higher education is insufficient to meet the needs of the development of the times.It is imperative to improve the training of information technology innovation talents and explore a new training model.This paper describes the general situation of the development of education in the field of information technology from a domestic and international perspective.It then analyzes the existing problems,explores new exploration models and implementation suggestions,and puts forward prospects at the end of the paper.
文摘Latent variable models can effectively determine the condition of essential rotating machinery without needing labeled data.These models analyze vibration data via an unsupervised learning strategy.Temporal preservation is necessary to obtain an informative latent manifold for the fault diagnosis task.In a temporalpreserving context,two approaches exist to develop a condition-monitoring methodology:offline and online.For latent variable models,the available training modes are not different.While many traditional methods use offline training,online training can dynamically adjust the latent manifold,possibly leading to better fault signature extraction from the vibration data.This study explores online training using temporal-preserving latent variable models.Within online training,there are two main methods:one focuses on reconstructing data and the other on interpreting the data components.Both are considered to evaluate how they diagnose faults over time.Using two experimental datasets,the study confirms that models from both training modes can detect changes in machinery health and identify faults even under varying conditions.Importantly,the complementarity of offline and online models is emphasized,reassuring their versatility in fault diagnostics.Understanding the implications of the training approach and the available model formulations is crucial for further research in latent variable modelbased fault diagnostics.
文摘Objective:To explore the effectiveness of the competency model for nursing managers in the training of newly appointed head nurses.Methods:Eighteen newly appointed head nurses from Yichang Central People’s Hospital,selected between August 2023 and July 2024,were chosen as the research subjects.Based on the nursing manager competency model,corresponding training programs were developed,and these 18 head nurses were trained accordingly.Results:After the training,all newly appointed head nurses passed the theoretical assessments,with a pass rate of 100.00%.The pass rates for two rounds of assessment were 100.00%and 94.54%,respectively.There was no significant difference in the performance evaluation scores between new and senior head nurses in terms of standardized services,nursing quality,nursing research and teaching,and patient satisfaction(P>0.05).Conclusion:The competency model for nursing managers has a significant effect on the training of newly appointed head nurses.It effectively enhances management awareness,facilitates role transition,and,to some extent,improves the management and leadership capabilities of head nurses.It holds high value for broader implementation.
文摘By analyzing the complementarity between smart agriculture and farming-reading education,and the advantages of organic combination of the two:addressing the challenges of agricultural modernization and cultivating new talents;enhancing students interest in learning and improving their sense of responsibility and mission;promoting the inheritance of traditional agricultural culture and modern agricultural technology innovation,the organic combination mode of smart agriculture and farming-reading education in the cultivation of new agricultural talents is explored.The analysis shows that it has the following applications in the cultivation of new agricultural talents:in terms of the integration of educational concepts,it combines the modern concept of smart agriculture with the traditional value of farming-reading education;in terms of constructing a curriculum system and adding courses related to smart agriculture,it retains basic courses in farming-reading education,and promotes interdisciplinary integration;in terms of practical teaching platforms,it establishes smart agriculture training bases and farming-reading education bases,and adopts a combination of virtual and real teaching methods;in terms of guiding innovation and entrepreneurship,it encourages students to participate in innovation and entrepreneurship projects,and strengthens industry-university-research cooperation;in terms of teacher team construction,it improves the quality of the teacher team and integrates educational resources both inside and outside the school.Moreover,its evaluation system and incentive mechanism are proposed:constructing a multi-dimensional evaluation system,combining quantitative and qualitative,process and result evaluation,and introducing the third-party evaluation;establishing scholarships and grants,providing support for innovation and entrepreneurship,conducting honor recognition and publicity,and offering career development support.
文摘The recent increase in the use of artificial intelligence has led to fundamental changes in the development of training and teaching methods for executive education. However, the success of artificial intelligence in regional centers for teaching and training professions will depend on the acceptance of this technology by young executive trainees. This article discusses the potential benefits of adopting AI in executive training institutions in Morocco, specifically focusing on CRMEF Casablanca Settat. Based on the Unified Theory of Acceptance and Use of Technology (UTAUT) (Venkatesh et al., 2003), this study proposes a model to identify the factors influencing the acceptance of artificial intelligence in regional centers for teaching professions and training in Morocco. To achieve this, a structural equation modeling approach was used to quantitatively describe the impact of each factor on AI adoption, utilizing data collected from 173 young executive trainees. The results indicate that perceived ease of use, perceived usefulness, trainer influence, and personal innovativeness influence the intention to use artificial intelligence. Our research provides managers of CRMEFs with a set of practical recommendations to enhance the implementation conditions of an artificial intelligence system. It aims to understand which factors should be considered in designing an artificial intelligence system within regional centers for teaching professions and training (CRMEFs).
文摘At present,as China vigorously develops new energy automobiles,the social demand for related talents has increased significantly,so the relevant institutions need to optimize and adjust the talent training mode to help the development of new energy automobiles.This paper summarizes the existing problems of China’s new energy automobile talent training mode,and analyzes the significance of the virtual simulation-based dual-creation talent training mode of new energy automobile and its specific implementation strategies,in order to provide references for the relevant personnel.
基金Reform and Practice of Talent Training Mode for Construction Engineering Technology Majors Serving the National Dual Carbon Strategy(Project No.Z233314)。
文摘Against the backdrop of global climate change and China’s“dual carbon”goals,the green transformation of the construction industry is imperative,and completing the transformation requires many dual carbon talents to support it.This article focuses on the construction engineering technology major in higher vocational education.It explores in depth the specific requirements for construction engineering technology talents in terms of professional knowledge,vocational skills,and literacy under the dual carbon mode.Based on this,corresponding dual carbon talent training courses are proposed,aiming to provide theoretical support and practical guidance for cultivating high-quality dual carbon talents that meet the needs of the new era.
基金Education and Teaching Reform and Practice Project of Guangdong Province Vocational Colleges Steering Committee for Integration of Production and Education in 2022“Research on the Application of OSCE Mode in Vocational Nursing Training Teaching”(Project No.2022CJRH14)。
文摘The objective of this study is to examine the application effect of the Objective Structured Clinical Examination(OSCE)model in the comprehensive practical training course for higher vocational nursing.Based on the evaluation dimensions of core nursing competencies,the OSCE framework was constructed with the clinical nursing work process as the main focus and supported by a nursing case database.A questionnaire survey revealed that over 80%of students in the experimental group were satisfied with the application of OSCE in the nursing comprehensive practical training course,and more than 90%believed that OSCE assessment enhanced their theoretical knowledge and practical skills.The OSCE was also considered helpful in transforming learned knowledge and skills into professional competencies.
文摘The current situation of European educational systems leads to the lack of historical precedents,considering that it implies the end of educational systems based on exclusion,configuring a new conception of educational systems that we are still not able to appreciate at their true value,because we were educated by and in the previous educational system.Lacking other references,we inevitably tend to compare current problems with previous situations,without understanding that the generalization of education to 100%of the population implies a qualitative change that modifies the objectives,forms of work,and the very essence of the educational system.This stage change,marked by the acceleration of social changes,including the pandemic situation,explains why,despite the best educational system so far,teachers with the best levels of training and a financial and material endowment unimaginable until recently,try a sense of crisis and a general feeling of confusion is manifested among the teachers and parents of the children,seeing that education is no longer what it used to be(Esteve,1998).The key to defuse this apparent paradox is in the tendency to judge the new education system by the mentality and standards of the former selective education,in which most of the adults present were educated.Thus,although we continue to apply to the new general education situation the parameters of the previous situation,in which the selective system excluded every year the students who had behavior problems in the class or had difficulties regarding the mastery of certain educational subjects(Esteve,1998).In this way,we are currently criticizing the school failure of a percentage of our students,without realizing that their schooling itself is a success,because,although their level is low,they had no level before.One speaks of a low educational level,when,in reality,the educational level is increasing—the general level of the people and the individual level of the best students(Baudelot&Establet,1990).Indeed,it is true that in our education systems there are students with a very low educational level,although even so,it is a success that they have some education because they used to be on the streets;and,in any case,the current education system produces a higher percentage of students with higher levels than the best students of the 90s.The main element of change,the most substantial transformation,is the elimination of exclusion,today having access to secondary education,alongside students with an excellent level.Thousands of children who were previously expelled from it,thus comply with the European measures of access to education for all children and young people.The previous educational system can be compared to a hospital from which the sickest were kicked out,and those who created behavioral problems were eliminated through the selective mechanisms of the system.However,teaching today is qualitatively different from what it was 20-30 years ago.First of all,because working with a homogeneous group of children as a result of the selection or dealing with all the children in a country,with social and personal problems,is not the same thing.That is why many general education teachers are social workers rather than teachers;and the new configuration of our educational systems requires secondary teachers to assume diverse educational roles rather than the traditional one of intellectual training.History cannot go backwards;to have better quality educational systems,the solution is not to return to the exclusionary system.Society asks teachers to make an effort to integrate;but,during this time,our society must support and revalue the activity of teachers in order not to put them in front of impossible tasks.The transition from a selective to a general education was based on the increase/amplification of compulsory schooling.For the first time in history,European educational systems have assumed the role of declaring secondary education compulsory.This decision implies a new stage,a definitive break from the previous reality in which secondary education was reserved for those who later attend a university.
文摘Neuromyelitis optica spectrum disorders are neuroinflammatory demyelinating disorders that lead to permanent visual loss and motor dysfunction.To date,no effective treatment exists as the exact causative mechanism remains unknown.Therefore,experimental models of neuromyelitis optica spectrum disorders are essential for exploring its pathogenesis and in screening for therapeutic targets.Since most patients with neuromyelitis optica spectrum disorders are seropositive for IgG autoantibodies against aquaporin-4,which is highly expressed on the membrane of astrocyte endfeet,most current experimental models are based on aquaporin-4-IgG that initially targets astrocytes.These experimental models have successfully simulated many pathological features of neuromyelitis optica spectrum disorders,such as aquaporin-4 loss,astrocytopathy,granulocyte and macrophage infiltration,complement activation,demyelination,and neuronal loss;however,they do not fully capture the pathological process of human neuromyelitis optica spectrum disorders.In this review,we summarize the currently known pathogenic mechanisms and the development of associated experimental models in vitro,ex vivo,and in vivo for neuromyelitis optica spectrum disorders,suggest potential pathogenic mechanisms for further investigation,and provide guidance on experimental model choices.In addition,this review summarizes the latest information on pathologies and therapies for neuromyelitis optica spectrum disorders based on experimental models of aquaporin-4-IgG-seropositive neuromyelitis optica spectrum disorders,offering further therapeutic targets and a theoretical basis for clinical trials.
基金the University of Transport Technology under the project entitled“Application of Machine Learning Algorithms in Landslide Susceptibility Mapping in Mountainous Areas”with grant number DTTD2022-16.
文摘This study was aimed to prepare landslide susceptibility maps for the Pithoragarh district in Uttarakhand,India,using advanced ensemble models that combined Radial Basis Function Networks(RBFN)with three ensemble learning techniques:DAGGING(DG),MULTIBOOST(MB),and ADABOOST(AB).This combination resulted in three distinct ensemble models:DG-RBFN,MB-RBFN,and AB-RBFN.Additionally,a traditional weighted method,Information Value(IV),and a benchmark machine learning(ML)model,Multilayer Perceptron Neural Network(MLP),were employed for comparison and validation.The models were developed using ten landslide conditioning factors,which included slope,aspect,elevation,curvature,land cover,geomorphology,overburden depth,lithology,distance to rivers and distance to roads.These factors were instrumental in predicting the output variable,which was the probability of landslide occurrence.Statistical analysis of the models’performance indicated that the DG-RBFN model,with an Area Under ROC Curve(AUC)of 0.931,outperformed the other models.The AB-RBFN model achieved an AUC of 0.929,the MB-RBFN model had an AUC of 0.913,and the MLP model recorded an AUC of 0.926.These results suggest that the advanced ensemble ML model DG-RBFN was more accurate than traditional statistical model,single MLP model,and other ensemble models in preparing trustworthy landslide susceptibility maps,thereby enhancing land use planning and decision-making.
基金supported and funded by the Deanship of Scientific Research at Imam Mohammad Ibn Saud Islamic University(IMSIU)(grant number IMSIU-RP23066).
文摘This study directs the discussion of HIV disease with a novel kind of complex dynamical generalized and piecewise operator in the sense of classical and Atangana Baleanu(AB)derivatives having arbitrary order.The HIV infection model has a susceptible class,a recovered class,along with a case of infection divided into three sub-different levels or categories and the recovered class.The total time interval is converted into two,which are further investigated for ordinary and fractional order operators of the AB derivative,respectively.The proposed model is tested separately for unique solutions and existence on bi intervals.The numerical solution of the proposed model is treated by the piece-wise numerical iterative scheme of Newtons Polynomial.The proposed method is established for piece-wise derivatives under natural order and non-singular Mittag-Leffler Law.The cross-over or bending characteristics in the dynamical system of HIV are easily examined by the aspect of this research having a memory effect for controlling the said disease.This study uses the neural network(NN)technique to obtain a better set of weights with low residual errors,and the epochs number is considered 1000.The obtained figures represent the approximate solution and absolute error which are tested with NN to train the data accurately.
基金Supported by National Natural Science Foundation of China,No.81874390 and No.81573948Shanghai Natural Science Foundation,No.21ZR1464100+1 种基金Science and Technology Innovation Action Plan of Shanghai Science and Technology Commission,No.22S11901700the Shanghai Key Specialty of Traditional Chinese Clinical Medicine,No.shslczdzk01201.
文摘BACKGROUND Rebleeding after recovery from esophagogastric variceal bleeding(EGVB)is a severe complication that is associated with high rates of both incidence and mortality.Despite its clinical importance,recognized prognostic models that can effectively predict esophagogastric variceal rebleeding in patients with liver cirrhosis are lacking.AIM To construct and externally validate a reliable prognostic model for predicting the occurrence of esophagogastric variceal rebleeding.METHODS This study included 477 EGVB patients across 2 cohorts:The derivation cohort(n=322)and the validation cohort(n=155).The primary outcome was rebleeding events within 1 year.The least absolute shrinkage and selection operator was applied for predictor selection,and multivariate Cox regression analysis was used to construct the prognostic model.Internal validation was performed with bootstrap resampling.We assessed the discrimination,calibration and accuracy of the model,and performed patient risk stratification.RESULTS Six predictors,including albumin and aspartate aminotransferase concentrations,white blood cell count,and the presence of ascites,portal vein thrombosis,and bleeding signs,were selected for the rebleeding event prediction following endoscopic treatment(REPET)model.In predicting rebleeding within 1 year,the REPET model ex-hibited a concordance index of 0.775 and a Brier score of 0.143 in the derivation cohort,alongside 0.862 and 0.127 in the validation cohort.Furthermore,the REPET model revealed a significant difference in rebleeding rates(P<0.01)between low-risk patients and intermediate-to high-risk patients in both cohorts.CONCLUSION We constructed and validated a new prognostic model for variceal rebleeding with excellent predictive per-formance,which will improve the clinical management of rebleeding in EGVB patients.
基金supported by Warren Alpert Foundation and Houston Methodist Academic Institute Laboratory Operating Fund(to HLC).
文摘Rare neurological diseases,while individually are rare,collectively impact millions globally,leading to diverse and often severe neurological symptoms.Often attributed to genetic mutations that disrupt protein function or structure,understanding their genetic basis is crucial for accurate diagnosis and targeted therapies.To investigate the underlying pathogenesis of these conditions,researchers often use non-mammalian model organisms,such as Drosophila(fruit flies),which is valued for their genetic manipulability,cost-efficiency,and preservation of genes and biological functions across evolutionary time.Genetic tools available in Drosophila,including CRISPR-Cas9,offer a means to manipulate gene expression,allowing for a deep exploration of the genetic underpinnings of rare neurological diseases.Drosophila boasts a versatile genetic toolkit,rapid generation turnover,and ease of large-scale experimentation,making it an invaluable resource for identifying potential drug candidates.Researchers can expose flies carrying disease-associated mutations to various compounds,rapidly pinpointing promising therapeutic agents for further investigation in mammalian models and,ultimately,clinical trials.In this comprehensive review,we explore rare neurological diseases where fly research has significantly contributed to our understanding of their genetic basis,pathophysiology,and potential therapeutic implications.We discuss rare diseases associated with both neuron-expressed and glial-expressed genes.Specific cases include mutations in CDK19 resulting in epilepsy and developmental delay,mutations in TIAM1 leading to a neurodevelopmental disorder with seizures and language delay,and mutations in IRF2BPL causing seizures,a neurodevelopmental disorder with regression,loss of speech,and abnormal movements.And we explore mutations in EMC1 related to cerebellar atrophy,visual impairment,psychomotor retardation,and gain-of-function mutations in ACOX1 causing Mitchell syndrome.Loss-of-function mutations in ACOX1 result in ACOX1 deficiency,characterized by very-long-chain fatty acid accumulation and glial degeneration.Notably,this review highlights how modeling these diseases in Drosophila has provided valuable insights into their pathophysiology,offering a platform for the rapid identification of potential therapeutic interventions.Rare neurological diseases involve a wide range of expression systems,and sometimes common phenotypes can be found among different genes that cause abnormalities in neurons or glia.Furthermore,mutations within the same gene may result in varying functional outcomes,such as complete loss of function,partial loss of function,or gain-of-function mutations.The phenotypes observed in patients can differ significantly,underscoring the complexity of these conditions.In conclusion,Drosophila represents an indispensable and cost-effective tool for investigating rare neurological diseases.By facilitating the modeling of these conditions,Drosophila contributes to a deeper understanding of their genetic basis,pathophysiology,and potential therapies.This approach accelerates the discovery of promising drug candidates,ultimately benefiting patients affected by these complex and understudied diseases.
基金supported by University of Macao,China,Nos.MYRG2022-00054-FHS and MYRG-GRG2023-00038-FHS-UMDF(to ZY)the Macao Science and Technology Development Fund,China,Nos.FDCT0048/2021/AGJ and FDCT0020/2019/AMJ and FDCT 0011/2018/A1(to ZY)Natural Science Foundation of Guangdong Province of China,No.EF017/FHS-YZ/2021/GDSTC(to ZY)。
文摘To investigate the mechanisms underlying the onset and progression of ischemic stroke,some methods have been proposed that can simultaneously monitor and create embolisms in the animal cerebral cortex.However,these methods often require complex systems and the effect of age on cerebral embolism has not been adequately studied,although ischemic stroke is strongly age-related.In this study,we propose an optical-resolution photoacoustic microscopy-based visualized photothrombosis methodology to create and monitor ischemic stroke in mice simultaneously using a 532 nm pulsed laser.We observed the molding process in mice of different ages and presented age-dependent vascular embolism differentiation.Moreover,we integrated optical coherence tomography angiography to investigate age-associated trends in cerebrovascular variability following a stroke.Our imaging data and quantitative analyses underscore the differential cerebrovascular responses to stroke in mice of different ages,thereby highlighting the technique's potential for evaluating cerebrovascular health and unraveling age-related mechanisms involved in ischemic strokes.