Polyamines play an important role in plant response to abiotic stress. S-adenosyl-1-methionine decarboxylase (SAMDC) is one of the key regulatory enzymes in the biosynthesis of polyamines. In order to better understan...Polyamines play an important role in plant response to abiotic stress. S-adenosyl-1-methionine decarboxylase (SAMDC) is one of the key regulatory enzymes in the biosynthesis of polyamines. In order to better understand the effect of regulation of polyamine biosynthesis on the shelf life improvement of litchi fruit, SAMDC cDNA isolated from Datura stramonium cloned in pBI121 was introduced into litchi genome by means of Agrobacterium tumefaciens through zygote disc transformation. Transgene and its expression are confirmed by Southern and Northern blot analyses, respectively. Transgenic plants expressing Datura SAMDC produced 1.7- to 2.4-fold higher levels of spermidine and spermine than wildtype plants under normal environmental condition, which indicated that the transgenic litchi presented an enhanced polyamines synthesis compared to wildtype plants. Our results demonstrated clearly that increasing polyamine biosynthesis in plants may be a means of creating improved fruit shelf life germplasm.展开更多
The interaction of regular quasi-monochromatic waves with a weakly submerged rectangular shelf is studied by means of CFD simulations. The fundamental incident wave frequency is kept constant for the full set of simul...The interaction of regular quasi-monochromatic waves with a weakly submerged rectangular shelf is studied by means of CFD simulations. The fundamental incident wave frequency is kept constant for the full set of simulated cases, while the incident wave amplitude is made increase progressively, so that the interaction with the shelf is dominated by almost inviscid non-linear flow for the smallest and by breaking for the highest incident waves. A parameter identification（PI） procedure is used to adapt a reduced model to the highly resolved time-space matrix of wave elevations obtained from the numerical simulations, on the weather and lee side respectively. In particular the wave number and the frequency of the component waves in the reduced model are left uncoupled, thus computed by the PI independently. The comparison of simulated data with experiments generally shows a very good agreement. Free/locked, incident/reflected, first/higher order wave components are quantified accurately by the PI and the energy transfer to super-harmonics is clearly evidenced. Moreover the results of the PI show clearly a very large increase in the phase speed of the higher order free waves on the lee side of the shelf, with increasing deviation from the linear behavior with increasing incident wave amplitude.展开更多
The effect of paleo-Pacific subduction on the geological evolution of the western Pacific and continental China is likely complex. Nevertheless, our analysis of the distribution of Mesozoic granitoids in the eastern c...The effect of paleo-Pacific subduction on the geological evolution of the western Pacific and continental China is likely complex. Nevertheless, our analysis of the distribution of Mesozoic granitoids in the eastern continental China in space and time has led us to an interesting conclusion： The basement of the continental shelf beneath East and South China Seas may actually be of exotic origin geologically unrelated to the continental lithosphere of eastern China. By accepting the notion that the Jurassic- Cretaceous granitoids in the region are genetically associated with western Pacific subduction and the concept that subduction may cease to continue only if the trench is being jammed, then the termination of the granitoid magmatism throughout the vast region at -88±2 Ma manifests the likelihood of ＂sudden＂, or shortly beforehand （- 100 Ma）, trench jam of the Mesozoic western Pacific subduction. Trench jam happens if the incoming ＂plate＂ or portion of the plate contains a sizeable mass that is too buoyant to subduct. The best candidate for such a buoyant and unsubductable mass is either an oceanic plateau or a micro-continent. We hypothesize that the basement of the Chinese continental shelf represents such an exotic, buoyant and unsubductable mass, rather than seaward extension of the continental lithosphere of eastern China. The locus of the jammed trench （i.e., the suture） is predictably located on the shelf in the vicinity of, and parallel to, the arc-curved coastal line of the southeast continental China. It is not straightforward to locate the locus in the northern section of the East China Sea shelf because of the more recent （〈20 Ma） tectonic re-organization associated with the opening of the Sea of Japan. We predict that the trench jam at - 100 Ma led to the re-orientation of the Pacific plate motion in the course of NNW direction as inferred from the age-progressive Emperor Seamount Chain of Hawaiian hotspot origin （its oldest unsubdued Meiji and Detroit seamounts are -82 Ma）, making the boundary between the Pacific plate and the newly accreted plate of eastern Asia transform fault at the location east of the continental shelf of exotic origin. This explains the apparent-40 Myr magmatic gap from - 88 to - 50 Ma prior to present-day western Pacific subduction initiation. We propose that basement penetration drilling on well-chosen sites is needed to test the hypothesis in order to reveal the true nature of the Chinese continental shelf basement. This testing becomes critical and cannot longer be neglected in order to genuinely understand the tectonic evolution of the western Pacific and its effect on the geology of eastern China since the Mesozoic, including the cratonic lithosphere thinning, related magmatism/mineralization, and the mechanism of the subsequent South China Sea opening, while also offering novel perspectives on aspects of the plate tectonics theory. We also suggest the importance of future plate tectonic reconstruction of the western Pacific to consider the nature and histories of the Chinese continental shelf of exotic origin as well as the probable transform plate boundary from - 100 to -50 Ma. Effort is needed to reveal the true nature and origin of the - 88 ± 2 Ma granitic gneisses in Taiwan and the 110-88 Ma granitoids on the Hainan Island.展开更多
文摘Polyamines play an important role in plant response to abiotic stress. S-adenosyl-1-methionine decarboxylase (SAMDC) is one of the key regulatory enzymes in the biosynthesis of polyamines. In order to better understand the effect of regulation of polyamine biosynthesis on the shelf life improvement of litchi fruit, SAMDC cDNA isolated from Datura stramonium cloned in pBI121 was introduced into litchi genome by means of Agrobacterium tumefaciens through zygote disc transformation. Transgene and its expression are confirmed by Southern and Northern blot analyses, respectively. Transgenic plants expressing Datura SAMDC produced 1.7- to 2.4-fold higher levels of spermidine and spermine than wildtype plants under normal environmental condition, which indicated that the transgenic litchi presented an enhanced polyamines synthesis compared to wildtype plants. Our results demonstrated clearly that increasing polyamine biosynthesis in plants may be a means of creating improved fruit shelf life germplasm.
基金The "Programma Attuativo Regionale del Fondo per lo Sviluppo e la Coesione (PAR FSC 2007-2013) Linea 3.1.2" is acknowledged for providing the support of the OpenViewSHIP Project
文摘The interaction of regular quasi-monochromatic waves with a weakly submerged rectangular shelf is studied by means of CFD simulations. The fundamental incident wave frequency is kept constant for the full set of simulated cases, while the incident wave amplitude is made increase progressively, so that the interaction with the shelf is dominated by almost inviscid non-linear flow for the smallest and by breaking for the highest incident waves. A parameter identification（PI） procedure is used to adapt a reduced model to the highly resolved time-space matrix of wave elevations obtained from the numerical simulations, on the weather and lee side respectively. In particular the wave number and the frequency of the component waves in the reduced model are left uncoupled, thus computed by the PI independently. The comparison of simulated data with experiments generally shows a very good agreement. Free/locked, incident/reflected, first/higher order wave components are quantified accurately by the PI and the energy transfer to super-harmonics is clearly evidenced. Moreover the results of the PI show clearly a very large increase in the phase speed of the higher order free waves on the lee side of the shelf, with increasing deviation from the linear behavior with increasing incident wave amplitude.
基金supported by the National Natural Science Foundation of China(41130314,91014003)Chinese Academy of Sciences Innovation(Y42217101L),grants from Regional and Local Authorities(Shandong Province and City of Qingdao)+1 种基金supported by National Oceanography Laboratory in Qingdaosupported by the National Natural Science Foundation of China(NSFC)
文摘The effect of paleo-Pacific subduction on the geological evolution of the western Pacific and continental China is likely complex. Nevertheless, our analysis of the distribution of Mesozoic granitoids in the eastern continental China in space and time has led us to an interesting conclusion： The basement of the continental shelf beneath East and South China Seas may actually be of exotic origin geologically unrelated to the continental lithosphere of eastern China. By accepting the notion that the Jurassic- Cretaceous granitoids in the region are genetically associated with western Pacific subduction and the concept that subduction may cease to continue only if the trench is being jammed, then the termination of the granitoid magmatism throughout the vast region at -88±2 Ma manifests the likelihood of ＂sudden＂, or shortly beforehand （- 100 Ma）, trench jam of the Mesozoic western Pacific subduction. Trench jam happens if the incoming ＂plate＂ or portion of the plate contains a sizeable mass that is too buoyant to subduct. The best candidate for such a buoyant and unsubductable mass is either an oceanic plateau or a micro-continent. We hypothesize that the basement of the Chinese continental shelf represents such an exotic, buoyant and unsubductable mass, rather than seaward extension of the continental lithosphere of eastern China. The locus of the jammed trench （i.e., the suture） is predictably located on the shelf in the vicinity of, and parallel to, the arc-curved coastal line of the southeast continental China. It is not straightforward to locate the locus in the northern section of the East China Sea shelf because of the more recent （〈20 Ma） tectonic re-organization associated with the opening of the Sea of Japan. We predict that the trench jam at - 100 Ma led to the re-orientation of the Pacific plate motion in the course of NNW direction as inferred from the age-progressive Emperor Seamount Chain of Hawaiian hotspot origin （its oldest unsubdued Meiji and Detroit seamounts are -82 Ma）, making the boundary between the Pacific plate and the newly accreted plate of eastern Asia transform fault at the location east of the continental shelf of exotic origin. This explains the apparent-40 Myr magmatic gap from - 88 to - 50 Ma prior to present-day western Pacific subduction initiation. We propose that basement penetration drilling on well-chosen sites is needed to test the hypothesis in order to reveal the true nature of the Chinese continental shelf basement. This testing becomes critical and cannot longer be neglected in order to genuinely understand the tectonic evolution of the western Pacific and its effect on the geology of eastern China since the Mesozoic, including the cratonic lithosphere thinning, related magmatism/mineralization, and the mechanism of the subsequent South China Sea opening, while also offering novel perspectives on aspects of the plate tectonics theory. We also suggest the importance of future plate tectonic reconstruction of the western Pacific to consider the nature and histories of the Chinese continental shelf of exotic origin as well as the probable transform plate boundary from - 100 to -50 Ma. Effort is needed to reveal the true nature and origin of the - 88 ± 2 Ma granitic gneisses in Taiwan and the 110-88 Ma granitoids on the Hainan Island.
