It has been more than 20 years since the first batch of transgenic fish was produced. Five stable germ-line transmitted growth hormone (GH) transgenic fish lines have been generated. This paper reviews the mechanisms ...It has been more than 20 years since the first batch of transgenic fish was produced. Five stable germ-line transmitted growth hormone (GH) transgenic fish lines have been generated. This paper reviews the mechanisms of integration and gene targeting of the transgene, as well as the viability, reproduction and transgenic approaches for the reproductive containment of GH-transgenic fish. Further, we propose that it should be necessary to do the following studies, in particularly, of the breeding of transgenic fish: to assess the fitness of transgenic fish in an aqueous environment with a large space and a complex structure; and to develop a controllable on-off strategy of reproduction in transgenic fish.展开更多
There is growing demand for biotechniques in oceanobiological and hydrobiological studies.Immunohistochemistry(IHC)combines anatomical,immunological and biochemical techniques to identify specific antigen-antibody int...There is growing demand for biotechniques in oceanobiological and hydrobiological studies.Immunohistochemistry(IHC)combines anatomical,immunological and biochemical techniques to identify specific antigen-antibody interactions.Detecting the target antigen with labeled antibodies is a multi-step process that requires optimization at every level to maximize the signal detection.Here we use zebrafish samples and optimize each IHC step,particularly antigen retrieval,transgenic reporter double staining and background counterstaining.The resulting images with high signal intensity lead us to recommend the resulting protocols for experiments with fish.展开更多
基金supported by the Development Plan of the State Key Fun-damental Research of China (Grant Nos. 2007CB109205, 2007CB109206)the National Natural Science Foundation of China (Grant No. 30930069)
文摘It has been more than 20 years since the first batch of transgenic fish was produced. Five stable germ-line transmitted growth hormone (GH) transgenic fish lines have been generated. This paper reviews the mechanisms of integration and gene targeting of the transgene, as well as the viability, reproduction and transgenic approaches for the reproductive containment of GH-transgenic fish. Further, we propose that it should be necessary to do the following studies, in particularly, of the breeding of transgenic fish: to assess the fitness of transgenic fish in an aqueous environment with a large space and a complex structure; and to develop a controllable on-off strategy of reproduction in transgenic fish.
基金This work was supported in part by the International Cooperation Initiative Program of Shanghai Ocean University(A2302110002)to J.YanShanghai Universities Firstclass Disciplines Project of Fisheries.
文摘There is growing demand for biotechniques in oceanobiological and hydrobiological studies.Immunohistochemistry(IHC)combines anatomical,immunological and biochemical techniques to identify specific antigen-antibody interactions.Detecting the target antigen with labeled antibodies is a multi-step process that requires optimization at every level to maximize the signal detection.Here we use zebrafish samples and optimize each IHC step,particularly antigen retrieval,transgenic reporter double staining and background counterstaining.The resulting images with high signal intensity lead us to recommend the resulting protocols for experiments with fish.
