Effects of Volsartan on Transmural Heterogeneous Changes of Transient Outward Potassium Currents in Hypertrophic Cardiomyocytes in Rabbits

The transmural heterogeneous changes of transient outward potassium currents (Ito) in rabbit hypertrophic cardiaomyocytes and the effects of long-term prophylactic treatment with volsartan were investigated. Rabbits were divided into hypertrophy group (left ventricular hypertrophy induced by partial ligation of abdominal aorta), vol-treated group (volsartan was administrated after the ligation), and control group (sham operated). Myocytes were isolated by a two-step enzymatical method. The sub-endocardial (Endo) and sub-epicardium (Epi) tissues were separated from midmyocardium (Mid) with a razor. Whole-cell patch-clamp technique was used to record potassium currents. The results showed that membrane capacitance was larger in hypertrophic cells than those in control and vol-treated cells (P<0.01 vs control cells, n=30). The densities of Ito in hypertrophic cells were reduced by sub-epicardium (Epi) (27.8±2.9) %, midmyocardium (Mid) (41.0±4.7) %, and sub-endocardium (Endo) (20.3±3.4) % compared with those in control cells. The decrease of Ito density was more pronounced in Mid than in Epi and Endo (P<0.01 vs Epi or Endo). There were no significant differences in Ito densities between vol-treated group and control group in three layers separately. In conclusion, volsartan can inhibit the transmural heterogeneous changes of Ito in left ventricular hypertrophic cardiomyocytes in rabbit.

Single-event-transient effect in nanotube tunnel field-effect transistor with bias-induced electron–hole bilayer

The single event transient(SET)effect in nanotube tunneling field-effect transistor with bias-induced electron–hole bilayer(EHBNT-TFET)is investigated by 3-D TCAD simulation for the first time.The effects of linear energy transfer(LET),characteristic radius,strike angle,electrode bias and hit location on SET response are evaluated in detail.The simulation results show that the peak value of transient drain current is up to 0.08 m A for heavy ion irradiation with characteristic radius of 50 nm and LET of 10 Me V·cm^(2)/mg,which is much higher than the on-state current of EHBNTTFET.The SET response of EHBNT-TFET presents an obvious dependence on LET,strike angle,drain bias and hit location.As LET increases from 2 Me V·cm^(2)/mg to 10 Me V·cm^(2)/mg,the peak drain current increases monotonically from 0.015 mA to 0.08 mA.The strike angle has an greater impact on peak drain current especially for the smaller characteristic radius.The peak drain current and collected charge increase by 0.014 mA and 0.06 fC,respectively,as the drain bias increases from 0.1 V to 0.9 V.Whether from the horizontal or the vertical direction,the most sensitive hit location is related to wt.The underlying physical mechanism is explored and discussed.

Effect of transient current bonding on interfacial reaction in Ag-coated graphene Sn-Ag-Cu composite solder joints

To address the problem of floating and aggregation of Ag-GNSs in the molten pool during the traditional reflow soldering process,Cu/SAC/Ag-GNSs/Cu sandwich joints were prepared under an applied current density(1.0×10^(4) A/cm^(2))for a few hundred milliseconds to produce Ag-coated graphene-reinforced Sn-Ag-Cu(SAC/AgGNSs)solder joints.The experimental results showed that Ag-GNSs were homogenously dispersed in the solder joints,providing more Cu6 Sn5 grain nucleation sites,which refined these grains and reduced the thickness difference at the anode and cathode.In addition,the Cu6 Sn5 morphology changed from rod-like to plate-shaped because of the uniform distribution of Ag-GNSs and constitutional supercooling.The significantly increased shear strength of the transient current bonding and the change in the fracture mechanism were due to the uniformly distributed Ag-GNSs and the microstructural changes.

Analytical solutions of transient pulsed eddy current problem due to elliptical electromagnetic concentrative coils

The electromagnetic concentrative coils are indispensable in the functional magnetic stimulation and have potential applications in nondestructive testing. In this paper, we propose a figure-8-shaped coil being composed of two arbitrary oblique elliptical coils, which can change the electromagnetic concentrative region and the magnitude of eddy current density by changing the elliptical shape and/or spread angle between two elliptical coils. Pulsed current is usually the excitation source in the functional magnetic stimulation, so in this paper we derive the analytical solutions of transient pulsed eddy current field in the time domain due to the elliptical concentrative coil placed in an arbitrary position over a half-infinite plane conductor by making use of the scale-transformation, the Laplace transform and the Fourier transform are used in our derivation. Calculation results of field distributions produced by the figure-8-shaped elliptical coil show some behaviours as follows： 1） the eddy currents are focused on the conductor under the geometric symmetric centre of figure-8-shaped coil; 2） the greater the scale factor of ellipse is, the higher the eddy current density is and the wider the concentrative area of eddy current along y axis is; 3） the maximum magnitude of eddy current density increases with the increase of spread angle. When spread angle is 180°, there are two additional reverse concentrative areas on both sides of x axis.

Characteristics of Transient Outward Potassium Channel Exposed to 3 mT Static Magnetic Field

Acutely isolated mouse hippocampal CA3 pyramidal neurons were exposed to 3 mT static magnetic field,and the characteristics of transient outward K+ channel were studied using the whole-cell patch-clamp technique.The experiment revealed that the amplitude of transient outward potassium channel current was reduced.The maximum activated current densities of control group and exposure group were 163.62±20.68 pA/pF and 98.74±16.57 pA/pF(n=12,P<0.01) respectively.The static magnetic field exposure affected the activation and inactivation process of transient outward potassium channel current.Due to the magnetic field exposure,the half-activation voltage of the activation curves changed from 5.59±1.96 mV to 27.87±7.24 mV(n=12,P<0.05) ,and the slope factor changed from 19.43±2.11 mV to 25.87±4.22 mV(n=12,P<0.05) .The half-inactivation voltage of the inactivation curves also changed from-56.09±0.89 mV to-57.16±1.10 mV(n=12,P>0.05) and the slope factor of the inactivation curves from 8.69±0.80 mV to 10.87±1.02 mV(n=12,P<0.05) .The results show that the static magnetic field can change the characteristics of transient outward K+ channel,and affect the physiological functions of neurons.

New Method for 3D Transient Eddy Current Field Calculation and Its Application in Magneto-Acoustic Tomography

A new method of 3D transient eddy current field calculation is proposed. The Maxwell equations with time component elimination （METCE） are derived under the assumption of magnetic quasi static approximation, especially for the sample of low conductivity. Based on METCE, we deduce a more efficient reconstruction algorithm of a 3D transient eddy current field. The computational burden is greatly reduced through the new algorithm, and the computational efficiency is improved. This new algorithm decompounds the space-time variables into two individual variables. The idea is to solve the spatial vector component firstly, and then multiply it by the corresponded time component. The iterative methods based on METCE are introduced to recover the distribution of conductivity in magneto-acoustic tomography. The reconstructed images of conductivity are consistent with the original distribution, which validate the new method.

Heavy Ion and Laser Microbeam Induced Current Transients in SiGe Heterojunction Bipolar Transistor

Silicon-germanium （SiGe） hereto-junction bipolar transistor current transients induced by pulse laser and heavy iron are measured using a real-time digital oscilloscope. These transients induced by pulse laser and heavy iron exhibit the same waveform and charge collection time except for the amplitude of peak current. Different laser energies and voltage biases under heavy ion irradiation also have impact on current transient, whereas the waveform remains unchanged. The position-correlated current transients suggest that the nature of the current transient is controlled by the behavior of the C/S junction.

Transient Current Behaviour for Iron in 3.5%NaCl and 3.5%NaCl+ 1%NaNO_2 Solutions during Corrosion Fatigue

The transient current behaviour for Iron in 3.5%NaCl and 3.5%NaCl +1%NaNO2 solutions during corrosion fatigue (CF) process has been investigated at different given strain amplitudes and strain rates. The results show that elastic strain has little contribution to material dissolution. The elastic tension strain results in the decrease in the transient current, while the elastic compression strain increases the transient current. Compared to the elastic deformation, plastic deformation affects material dissolution evidently For iron in 3.5%NaCl solution, the strain amplitude plays a dominant role in the dissolution process accelerated by the plastic strain, while in 3.5%NaCl+1%NaNO2 solution, both the strain amplitude and strain rate play an important role in this process. In this paper, the effect of the elastic deformation on the material dissolution and the relation between the tension and compression current peak values under the plastic cycle deformation are discussed

A novel transient rotor current control scheme of a doubly-fed induction generator equipped with superconducting magnetic energy storage for voltage and frequency support

A novel transient rotor current control scheme is proposed in this paper for a doubly-fed induction generator（DFIG）equipped with a superconducting magnetic energy storage（SMES） device to enhance its transient voltage and frequency support capacity during grid faults. The SMES connected to the DC-link capacitor of the DFIG is controlled to regulate the transient dc-link voltage so that the whole capacity of the grid side converter（GSC） is dedicated to injecting reactive power to the grid for the transient voltage support. However, the rotor-side converter（RSC） has different control tasks for different periods of the grid fault. Firstly, for Period I, the RSC injects the demagnetizing current to ensure the controllability of the rotor voltage. Then, since the dc stator flux degenerates rapidly in Period II, the required demagnetizing current is low in Period II and the RSC uses the spare capacity to additionally generate the reactive（priority） and active current so that the transient voltage capability is corroborated and the DFIG also positively responds to the system frequency dynamic at the earliest time. Finally, a small amount of demagnetizing current is provided after the fault clearance. Most of the RSC capacity is used to inject the active current to further support the frequency recovery of the system. Simulations are carried out on a simple power system with a wind farm. Comparisons with other commonly used control methods are performed to validate the proposed control method.

EFFECTS OF THE HOE 694 ON TRANSIENT INWARD CURRENT AND NA^+- CA^(2+) EXCHANGE IN GUINEA PIG CARDIOMYOCYTES

To determine the effects of HOE 694, a new and potent Na＋- H＋ exchanger blocker, on transient inward current (Iti) and Na＋- Ca2＋ exchange during hypoxia- reoxygenation in guinea pig cardiomyocytes. Methods. Cardiomyocytes were isolated from adult guinea pig ventricle. Experiment was performed in an experimental chamber that allowed the cells to be exposed to a sufficiently low O2 pressure. The cells were subjected to hypoxia and reoxygenation. The ionic currents were studied with patch clamp technique. Results. In the absence of HOE 694, hypoxia- reoxygenation induced Iti in 12 of 15 experiments; but in cardiomyocytes pretreated with HOE 694 (10～ 50μ mol/L), the incidence of Iti observed during reoxygenation was reduced to 5 of 11 experiments and 3 of 10 experiments, P Conclusions. Blockade of the Na＋- H＋ exchange by HOE 694 could reduce Ca2＋ overload upon hypoxia- reoxygenation, and inhibition of Na＋- H＋ exchange may also indirectly decrease Na＋- Ca2＋ exchange activity during hypoxia.

Different suppressive effect of lidocaine on persistent Na^+ current and transient Na^+ current in dorsal root ganglion neurons

Objective ：To investigate the different suppressive effect of lidocaine on persistent Na^＋ current and transient Na^＋ current in injured or uninjured dorsal root ganglion neurons. Methods： Totally 23 SD rats were randomly divided into 2 groups： control group （n： 10） and chronically compressed DRG （dorsal root ganglion） group （CCD group, n= 13）. Rats were anesthetized and DRG was isolated. Single DRG neuron was isolated by enzymatic disassociation method. Persistent Na^＋ current （INap） and transient Na^＋ current （INaT） were elicited in voltage clamp mode. Results： The presence of INap was testified in most DRG neurons （38/46 neurons in CCD group and 31/39 neurons in control group, P〉0. 05）. However, the cur- rent density of INap in CCD group （4. 6±0. 6 pA/pF, n=38 neurons） was greater than that in control group （2.5±0.4 pA/pF, n=31 neurons） （P〈0. 05）. The characteristics of INap was observed and found that INap could he blocked by 0.2 μmol/L tetrodotoxin easily. Furthermore, the does-effect relationship of lidocaine on INaP and IN.T were also examined. INaP and IN.T were suppressed by different concentrations of li- docaine, the range for INap was 5-20 μmol/L and for INaT was 0. 05-2 mmol/L. Conclusion： INap and INaT were suppressed by different concentrations of lidocaine. INap was suppressed by very low concentration of lidocaine （5-20 μmol/L）. However, INaT could only be blocked by high concentration of lidocaine （0.05-2 mmol/L）.

THE THEORY OF CURRENT TRANSIENTS AT TUBULAR ELECTRODES IN A FLOWING FLUID

A theoretical equation is developed which describes the response of the current transients to a constant potential at tubular electrodes for a reversible electrode reaction in the flowing fluid.

THE VERIFICATION OF THE CURRENT TRANSIENT EQUATION AT TUBULAR ELECTRODES IN A FLOWING FLUID UNDER POTENTIAL STEP

The equation derived for the response of the current transients in the flowing fluid is verified experimentally.

The simulation research for transient current variation in short circuit fault of urban rail transportation

Urban rail transit is one of the most important way for urban residents. However, frequent power failure, especially the short fault hinders the safe and stable operation of rail transit. The research of the transient variation of line electrical parameters in short circuit fault is the basis of researches for technology of line protection and short circuit fault location. Based on Matlab/Simulink, a 24-pulse rectifier circuit model is established, the resistance and inductance value of the catenary and rail network are calculated. The short Circuit fault simulation model of DC traction power supply system is established. The short-circuit fault of the traction network at close and distant points are simulated, the transient variation values of fault current with the different fault distance are analyzed. The simulation results show that the transient current peak of the nearby short circuit is oscillatory and convergent due to the nonlinear devices, which proves the accuracy of the model and provides a reference for the precise configuration of the line protection equipment.

Winding Function Theory Based Thrust Calculation on Nested-loop Secondary Linear Machine Adapted to Linear Metro

With advantages of strong drive capability,nested-loop secondary linear machine(NLS-LM)has great potentiality in linear metro.For its secondary structure with multiple loops,it is difficult to calculate the electromagnetic thrust of NLS-LM reasonably.Hence,in this paper,one thrust calculation method is proposed considering variable loop inductance and transient loop current.Firstly,to establish the secondary winding function,the modeling domain is confined to a limited range,and the equivalent loop span is employed by analyzing the coupling relationship between primary and secondary.Then,in order to obtain the secondary flux density,the transient secondary current is solved based on the loop impedance and induced voltage.Finally,the electromagnetic thrust can be calculated reasonably by the given primary current sheet and the calculated secondary flux density.Comprehensive simulations and experiments have demonstrated the effectiveness of the proposed method.

Study on Electromagnetic Force and Vibration of Turbogenerator End Windings under Impact Load(Ⅰ): Analysis of Electromagnetic Force of End Windings under Impact Load

In this paper, the boundary value problem (BVP) of 3 D transient eddy current field in the end region in the case that the generator is affected by impact load is specified. Besides, ways to implement discrete methods in both time domain and space domain during the solution of the problem are investigated. The Crank Nicolson scheme is utilized to attain the iterative format of time differential, after taking factors that can ensure both computation precision and stability into consideration. In this paper, the magnetic distribution in the end region of a turbogenerator in the case that the generator is affected by impact load is specified. As a result, it provides foundation for further study of electromagnetic force and electromagnetic vibration in the end region of the turbogenerator.

Application of fractal theory in detecting low current faults of power distribution system in coal mines

Single-phase low current grounding faults areoften seen in power distribution system of coal mines.These faults are difficult to reliably identify.We propose a new method of single-phase ground fault protection based upon a discernible matrix of the fractal dimension associated with line currents.The method builds on existing selective protection methods.Faulted feeders are distinguished using differences in the zero-sequence transient current fractal dimension.The current signals were first processed through a fast Fourier transform and then the characteristics of a faulted line were identified using a discernible matrix.The method of calculation is illustrated.The results show that the method involves simple calculations, is easy to do and is highly accurate.It is, therefore, suitable for distribution networks having different neutral grounding modes.

The advanced multi-functional carbon dots in photoelectrochemistry based energy conversion

Carbon dots(CDs),as a unique zero-dimensional member of carbon materials,have attracted numerous attentions for their potential applications in optoelectronic,biological,and energy related fields.Recently,CDs as catalysts for energy conversion reactions under multi-physical conditions such as light and/or electricity have grown into a research frontier due to their advantages of high visible light utilization,fast migration of charge carriers,efficient surface redox reactions and good electrical conductivity.In this review,we summarize the fabrication methods of CDs and corresponding CD nanocomposites,including the strategies of surface modification and heteroatom doping.The properties of CDs that concerned to the photo-and electro-catalysis are highlighted and detailed corresponding applications are listed.More importantly,as new non-contact detection technologies,transient photo-induced voltage/current have been developed to detect and study the charge transfer kinetics,which can sensitively reflect the complex electron separation and transfer behavior in photo-/electro-catalysts.The development and application of the techniques are reviewed.Finally,we discuss and outline the major challenges and opportunities for future CD-based catalysts,and the needs and expectations for the development of novel characterization technologies.

Role of calcium mobilization in the regulation of spontaneous transient outward currents in porcine coronary artery myocytes

The purpose of the present study was to further study the characteristics and regulation of spontaneous transient outward currents(STOCs)in freshly isolated porcine coronary artery smooth muscle cells(ASMCs).STOCs were recorded using the perforated whole-cell patch-clamp configuration.STOCs were voltage-dependent and superimposed stochastically onto whole-cell Ca^(2+)-activated-K+(BKCa)currents.Charybdotoxin(ChTX,200 nmol/L),a selective blocker of BKCa channels,completely inhibited STOCs within 10 min.STOCs activity was greatly suppressed when extracellular Ca^(2+)concentration decreased from 1.8 mmol/L to 200 nmol/L,further removal of Ca^(2+)abolished STOCs activity.Ca^(2+)ionophore A23187(10μmol/L)increased STOCs activity significantly.Verapamil(20μmol/L)and CdCl2(200μmol/L),two kinds of organic L-type voltage-dependent Ca^(2+)channels(L-VDCCs)antagonists,had little effect on STOCs.In addition,the ryanodine receptors(RyRs)agonist caffeine(5 mmol/L)significantly activated STOCs.Application of ryanodine(50μmol/L)to block RyRs abolished STOCs,subsequent washout of ryanodine or application of caffeine failed to reproduce STOCs activity.Inhibition of inositol 1,4,5-trisphosphate receptors(IP_(3)Rs)by 2APB(40μmol/L)greatly suppressed the activity of STOCs,application of caffeine(5 mmol/L)in the presence of 2APB caused a burst of outward currents followed by inhibition of STOCs.These results suggest that STOCs in porcine coronary ASMCs are mediated by BKCa channels.Extracellular Ca^(2+)is essential for STOCs activity,while Ca^(2+)entry through L-VDCCs has little effect on STOCs.Intracellular Ca^(2+)release induced by RyRs is responsible for the regulation of STOCs,whereas IP_(3)Rs might also be involved.

Two outward potassium current types are expressed during the neural differentiation of neural stem cells

The electrophysiological properties of potassium ion channels are regarded as a basic index for determining the functional differentiation of neural stem cells. In this study, neural stem cells from the hippocampus of newborn rats were induced to differentiate with neurotrophic growth factor, and the electrophysiological properties of the voltage-gated potassium ion channels were observed. Immunofluorescence staining showed that the rapidly proliferating neural stem cells formed spheres in vitro that expressed high levels of nestin. The differentiated neurons were shown to express neuron-specific enolase. Flow cytometric analysis revealed that the neural stem cells were actively dividing and the percentage of cells in the S ＋ G2/M phase was high. However, the ratio of cells in the S ＋ G2/M phase decreased obviously as differentiation proceeded. Whole-cell patch-clamp re- cordings revealed apparent changes in potassium ion currents as the neurons differentiated. The potassium ion currents consisted of one transient outward potassium ion current and one delayed rectifier potassium ion current, which were blocked by 4-aminopyridine and tetraethylammonium, respectively. The experimental findings indicate that neural stem cells from newborn rat hippo- campus could be cultured and induced to differentiate into functional neurons under defined condi- tions in vitro. The differentiated neurons expressed two types of outward potassium ion cur＇ents similar to those of mature neurons in vivo.