We present a novel efficient approach in calculating induced transmembrane voltage（ITV） on cells based on transformation optics. As cell membrane is much thinner than the dimension of a typical cell, discretizing th...We present a novel efficient approach in calculating induced transmembrane voltage（ITV） on cells based on transformation optics. As cell membrane is much thinner than the dimension of a typical cell, discretizing the membrane needs numerous meshes. Using an anisotropic medium based on transformation optics, the thickness of the membrane can be exaggerated by at least one order, which eliminates rigorous mesh refinement and reduces unknowns greatly. The accuracy and efficiency of the proposed method are verified by a cylindrical cell model. Moreover, the influence on ITV with bound water（BW） layers is also studied. The results show that when cells are exposed to nanosecond electric field, BW layers should be rigorously considered in calculating ITV.展开更多
Pulsed electric field(PEF)technology is a highly promising non-thermal processing technique,which holds great potential for sterilizing liquid food.The effectiveness of this treatment depends on two crucial factors:pu...Pulsed electric field(PEF)technology is a highly promising non-thermal processing technique,which holds great potential for sterilizing liquid food.The effectiveness of this treatment depends on two crucial factors:pulse parameters and the structure of the processor.In this study,we focus on optimizing the shape of insulators within the PEF processor to improve the sterilization effect.This research provides a reliable technical reference for designing industrial equipment.The numerical simulation of multi-physics fields was employed to analyze the temperature,electric field,and fluid field distributions for different insulators.Ultimately,the geometric structure of the insulator was optimized by embedding a 0.7-mm arc.Sterilization experiments were then conducted on Escherichia coli and Staphylococcus aureus suspensions with conductivities similar to those of real grapefruit juice.The sterilization effect of the processor with the optimized structure and the maximum applied voltage was examined at the same driving frequency.The results indicate that compared to the original structure,there is an increase in withstanding voltage by 5–10 kV while maintaining significantly improved bactericidal effects at the same applied voltage.Furthermore,we preliminarily discussed the sterilization mechanism by combining electroporation theory with the electroporation threshold of S.aureus.展开更多
基金Project supported by the National Key Basic Research Program of China(Grant Nos.2013CB328900 and 2013CB328905)
文摘We present a novel efficient approach in calculating induced transmembrane voltage（ITV） on cells based on transformation optics. As cell membrane is much thinner than the dimension of a typical cell, discretizing the membrane needs numerous meshes. Using an anisotropic medium based on transformation optics, the thickness of the membrane can be exaggerated by at least one order, which eliminates rigorous mesh refinement and reduces unknowns greatly. The accuracy and efficiency of the proposed method are verified by a cylindrical cell model. Moreover, the influence on ITV with bound water（BW） layers is also studied. The results show that when cells are exposed to nanosecond electric field, BW layers should be rigorously considered in calculating ITV.
基金the Key R&D Program of Guangdong Province(No.2018B020239001),China.
文摘Pulsed electric field(PEF)technology is a highly promising non-thermal processing technique,which holds great potential for sterilizing liquid food.The effectiveness of this treatment depends on two crucial factors:pulse parameters and the structure of the processor.In this study,we focus on optimizing the shape of insulators within the PEF processor to improve the sterilization effect.This research provides a reliable technical reference for designing industrial equipment.The numerical simulation of multi-physics fields was employed to analyze the temperature,electric field,and fluid field distributions for different insulators.Ultimately,the geometric structure of the insulator was optimized by embedding a 0.7-mm arc.Sterilization experiments were then conducted on Escherichia coli and Staphylococcus aureus suspensions with conductivities similar to those of real grapefruit juice.The sterilization effect of the processor with the optimized structure and the maximum applied voltage was examined at the same driving frequency.The results indicate that compared to the original structure,there is an increase in withstanding voltage by 5–10 kV while maintaining significantly improved bactericidal effects at the same applied voltage.Furthermore,we preliminarily discussed the sterilization mechanism by combining electroporation theory with the electroporation threshold of S.aureus.
