[Objective] This study aimed to shorten the multiplication culture and root- ing culture periods of Rh. chrysanthum Pall. [Method] The Rh. chrysanthum Pall tis- sue culture plantlets collected from Changbai Mountain w...[Objective] This study aimed to shorten the multiplication culture and root- ing culture periods of Rh. chrysanthum Pall. [Method] The Rh. chrysanthum Pall tis- sue culture plantlets collected from Changbai Mountain were used as material, and the effects of different hormone combinations and coconut milk on the proliferation and differentiation of Rh. chrysanthum Pall tissue culture plantlets were investigated. In addition, the rooting medium and transplanting matrix for Rh. chrysanthum Pall tissue culture plantlets were explored. [Result] The medium composed of modified MS, iBA (3 mg/L) and ZT (1.5 mg/L) was the optimum medium for subculture mul- tiplication of Rh. chrysanthum Pall tissue culture plantlets. The multiplication multiple and average plant height were significantly improved by adding coconut milk into the medium (150 mg/L). [Conclusion] For Rh. chrysanthum Pall tissue culture plantlets, the optimum rooting culture medium was composed of modified MS (1/4) and IBA (5.0 mg/L), and the tissue culture plantlets began to root 8 d after the inoculation. The root induction treatment was carried out after a 15-d sand culture, and the suitable matrix was composed of tufty soil, humus soil and perlite (2:1:1) with a survival rate of 95.66%.展开更多
针对针式末端执行器夹持移栽后穴孔内基质残留较多的问题,设计了一种指铲式末端执行器,通过增大指铲与苗钵基质的接触面积,减少穴孔内基质的残留。通过静力学分析找出末端执行器提取苗钵后基质残留的原因:基质间的最大内聚力小于由基质...针对针式末端执行器夹持移栽后穴孔内基质残留较多的问题,设计了一种指铲式末端执行器,通过增大指铲与苗钵基质的接触面积,减少穴孔内基质的残留。通过静力学分析找出末端执行器提取苗钵后基质残留的原因:基质间的最大内聚力小于由基质与穴盘间的粘附力和苗钵重力合成的总阻力时,苗钵出现破裂,基质塌陷。基于离散单元法,通过EDEM(Enhanced discrete element method)仿真分析了指铲式末端执行器对土壤基质的抓取过程,发现随着土壤基质内聚力的提高基质残留的现象得到改善;将基质配比和含水率条件作为主要影响因素,通过组合测盘试验测量在不同基质配比和含水率试验条件下的粘附力和内聚力变化,寻找内聚力大于粘附力的基质配比和含水率条件。试验表明,当相对含水率为60%、基质配比为6∶3∶1(泥炭∶蛭石∶珍珠岩)时,内聚压强和粘附压强的差值最大,在该条件下,指铲式末端执行器对劣质苗钵穴孔基质的平均剔净率达到70.8%,优于其他作业条件,可剔除穴孔内大部分基质。展开更多
基金Supported by Students'Innovation and Entrepreneurship Training Program of Yanbian University in 2015(ydbksky2015252)~~
文摘[Objective] This study aimed to shorten the multiplication culture and root- ing culture periods of Rh. chrysanthum Pall. [Method] The Rh. chrysanthum Pall tis- sue culture plantlets collected from Changbai Mountain were used as material, and the effects of different hormone combinations and coconut milk on the proliferation and differentiation of Rh. chrysanthum Pall tissue culture plantlets were investigated. In addition, the rooting medium and transplanting matrix for Rh. chrysanthum Pall tissue culture plantlets were explored. [Result] The medium composed of modified MS, iBA (3 mg/L) and ZT (1.5 mg/L) was the optimum medium for subculture mul- tiplication of Rh. chrysanthum Pall tissue culture plantlets. The multiplication multiple and average plant height were significantly improved by adding coconut milk into the medium (150 mg/L). [Conclusion] For Rh. chrysanthum Pall tissue culture plantlets, the optimum rooting culture medium was composed of modified MS (1/4) and IBA (5.0 mg/L), and the tissue culture plantlets began to root 8 d after the inoculation. The root induction treatment was carried out after a 15-d sand culture, and the suitable matrix was composed of tufty soil, humus soil and perlite (2:1:1) with a survival rate of 95.66%.
文摘针对针式末端执行器夹持移栽后穴孔内基质残留较多的问题,设计了一种指铲式末端执行器,通过增大指铲与苗钵基质的接触面积,减少穴孔内基质的残留。通过静力学分析找出末端执行器提取苗钵后基质残留的原因:基质间的最大内聚力小于由基质与穴盘间的粘附力和苗钵重力合成的总阻力时,苗钵出现破裂,基质塌陷。基于离散单元法,通过EDEM(Enhanced discrete element method)仿真分析了指铲式末端执行器对土壤基质的抓取过程,发现随着土壤基质内聚力的提高基质残留的现象得到改善;将基质配比和含水率条件作为主要影响因素,通过组合测盘试验测量在不同基质配比和含水率试验条件下的粘附力和内聚力变化,寻找内聚力大于粘附力的基质配比和含水率条件。试验表明,当相对含水率为60%、基质配比为6∶3∶1(泥炭∶蛭石∶珍珠岩)时,内聚压强和粘附压强的差值最大,在该条件下,指铲式末端执行器对劣质苗钵穴孔基质的平均剔净率达到70.8%,优于其他作业条件,可剔除穴孔内大部分基质。