Analysis of the relationship between postoperative ophthalmic complications and dialysis time of prekidney transplantation

AIM: To determine the influence of the dialysis time before kidney transplantation on postoperative ophthalmic complications. METHODS: One hundred and eighty three patients who were given the follow-up after kidney transplantation were selected, including 124 males and 59 females. The dialysis time before kidney transplantation was (2.9 +/- 2.1) years. Among them, there were 93 cases having cadaveric renal transplantation and 90 cases having living relative renal transplantation. The conditions of ophthalmic complications in all the patients after kidney transplantation were investigated and the incidence rate on ophthalmic complications having different dialysis time before kidney transplantation was given Chi-square test and Chi-square linear trend test. RESULTS: Among 183 patients with kidney transplantation, 95 patients (51.9%) had at least one ophthalmic complication and the rest 88 patients (48.1%) had no significant abnormality at the eye region. The most common ophthalmic complications were pinguecula/conjunctival degeneration (31 cases), the following was caligo lentis (24 cases). The main manifestations were grayish white granule and plaque turbidity occurred in posterior capsule at the posterior pole of crystaline lens. The angulus iridocornealis of 5 patients (5.3%) with cataract and glaucoma were all open-angle through the detection by gonioscope. Through visual field examination, there were 2 patients with paracentral scotoma, 2 patients with arcuate scotoma and one case with nasal step. CONCLUSION: The experiments verify that the incidence of glaucomawas relates to the dialysis time before kidney transplantation, and the incidence rate might be higher if the dialysis time is longer.

Evaluating the Optimum Transplanting Time for Different Coarse Rice Genotypes under Semi-Arid Conditions of Faisalabad

Continuously changing climate and availability of different rice genotypes make it necessary to find optimum time of sowing as well as suitable variety for cultivation to get maximum productivity under a specific set of climatic conditions. A field study was carried out to search out the suitable rice transplanting time for four different coarse genotypes under the semi-arid environment of Faisalabad. The experiment was conducted at Agronomic Research Area, University of Agriculture, Faisalabad and was laid out in randomized complete block design (RCBD) with split plot arrangement keeping transplanting time in main plots while rice genotypes in subplots. Variability among treatments was measured by Fisher’s ANOVA (P ≤ 5%) and LSD test was applied to compare the differences among treatments’ means. The ANOVA indicated statistically significant differences among genotypes as well as transplanting dates irrespective of all studied traits while interactive effects of both were found to be non-significant. NIBGE-1 performed best with maximum paddy yield of 6.05 t/ha while KSK-434 performed poor with paddy yield of 2.78 t/ha. Increased paddy yield and yield related parameters of all rice genotypes were recorded where transplantation was done on 25th of June. Generally, paddy yield decreased with delaying the transplanting time. The results suggested that NIBGE-1 can perform better under the semi-arid conditions of Faisalabad and last week of June might be the optimum time for nursery transplantation. It can also be further elucidated that late transplanting causes yield reduction which could not be recommended among farmers.

Impact of cirrhosis-related complications on posttransplant survival in patients with acute-on-chronic liver failure

Background:Acute-on-chronic liver failure(ACLF)is a life-threatening syndrome defined as acute decompensation in patients with chronic liver disease.Liver transplantation(LT)is the most effective treatment.We aimed to assess the impact of cirrhosis-related complications pre-LT on the posttransplant prognosis of patients with ACLF.Methods:This was an observational cohort study conducted between January 2018 and December 2020.Clinical characteristics,cirrhosis-related complications at LT and patient survival post-LT were collected.All liver recipients with ACLF were followed for 1 year post-LT.Results:A total of 212 LT recipients with ACLF were enrolled,including 75(35.4%)patients with ACLF-1,64(30.2%)with ACLF-2,and 73(34.4%)with ACLF-3.The median waiting time for LT was 11(4-24)days.The most prevalent cirrhosis-related complication was ascites(78.8%),followed by hepatic encephalopathy(57.1%),bacterial infections(48.1%),hepatorenal syndrome(22.2%)and gastrointestinal bleeding(11.3%).Survival analyses showed that patients with complications at LT had a significantly lower survival probability at both 3 months and 1 year after LT than those without complications(all P<0.05).A simplified model was developed by assigning one point to each complication:transplantation for ACLF with cirrhosis-related complication(TACC)model.Risk stratification of TACC model identified 3 strata(≥4,=3,and≤2)with high,median and low risk of death after LT(P<0.001).Moreover,the TACC model showed a comparable ability for predicting the outcome post-LT to the other four prognostic models(chronic liver failure-consortium ACLF score,Chinese Group on the Study of Severe Hepatitis B-ACLF score,model for end-stage liver disease score and Child-Turcotte-Pugh score).Conclusions:The presence of cirrhosis-related complications pre-LT increases the risk of death post-LT in patients with ACLF.The TACC model based on the number of cirrhosis-related complications pre-LT could stratify posttransplant survival,which might help to determine transplant timing for ACLF.

Effects of Different Sowing and Transplanting Time on Quality and Economic Benefit of Tobacco Variety Yunyan 105

In order to provide references for popularization and application of tobacco variety Yunyan 105,a field experiment was conducted to explore the effects of different sowing and transplanting time on agronomic traits,economic benefit and quality of Yunyan 105. Results showed that the agronomic characters,quality traits and economic benefit of the tobacco were relatively fine with sowing time February 4-14 and transplanting time April 1-10. Index scores of the physical properties of tobacco leaves were greater than 80 scores with sowing time February 14 and transplanting time April 10. The comprehensive index of conventional chemical compositions was 0. 39-0. 73. The harmony of conventional chemical compositions of lower leaves was the best with the comprehensive index of 0. 59 when the sowing time and transplanting time were February 4 and April 1 respectively. As for the middle and upper leaves the comprehensive indexes were respectively 0. 71 and 0. 73 with the sowing time February 14 and transplanting time April 10. The sensory evaluation of smoking quality and economic benefit were February 14 sowing and April 10 transplanting better,its taste of the tobacco leaves was pure and mild,the irritancy and the strength was less,the aftertaste was better,and the output value reached the highest 4 271. 17 yuan/667 m^2. The various sowing and transplanting time had no significant influence on appearance quality of tobacco leaves. The suitable sowing and transplanting time of Yunyan 105 was February 4-14 and April1-10 respectively in Xuanwei tobacco-growing area.

When is The Best Time of Stem Cell Transplantation for Treating Acute Myocardial Infarction──A Brief Meta-analysis of Current Clinic Trials

The best time of stem cells transplantation for treating acute myocardial infarction (AMI) is still to be followed with interest and a focus issue for clinical cardiologist. A brief meta-analysis of clinical trials about timing-window and therapeutic effects of stem cell transplantation for treating AMI will be made out in this article.

Optimal time point for the transplantation of neural stem cells induced to differentiate with retinoic acid

Previous studies have demonstrated that differentiated neural stem cells （NSCs） are more suitable for transplantation than non-differentiated NSCs. In this study, NSCs were expanded in vitro for two passages, induced with retinoic acid to differentiate, and harvested between 1 6 days later. They were subsequently cultured in artificial cerebrospinal fluid for an additional 3 days, dudng which their growth and morphology was monitored. NSCs induced for 4 days exhibited a peak rate of cells differentiating into neurons and robust growth. Our results indicate that the optimal time point for transplanting NSCs is following a 4-day period of induced differentiation.

Optimal time for subarachnoid transplantation of neural progenitor cells in the treatment of contusive spinal cord injury

This study aimed to identify the optimal neural progenitor cell transplantation time for spinal cord injury in rats via the subarachnoid space. Cultured neural progenitor cells from 14-day embryonic rats, constitutively expressing enhanced green fluorescence protein, or media alone, were injected into the subarachnoid space of adult rats at 1 hour （acute stage）, 7 days （subacute stage） and 28 days （chronic stage） after contusive spinal cord injury. Results showed that grafted neural progenitor cells migrated and aggregated around the blood vessels of the injured region, and infiltrated the spinal cord parenchyma along the tissue spaces in the acute stage transplantation group. However, this was not observed in subacute and chronic stage transplantation groups. 04- and glial fibrillary acidic protein-positive cells, representing oligodendrocytes and astrocytes respectively, were detected in the core of the grafted cluster attached to the cauda equina pia surface in the chronic stage transplantation group 8 weeks after transplantation. Both acute and subacute stage transplantation groups were negative for 04 and glial fibrillary acidic protein cells. Basso, Beattie and Bresnahan scale score comparisons indicated that rat hind limb locomotor activity showed better recovery after acute stage transplantation than after subacute and chronic transplantation. Our experimental findings suggest that the subarachnoid route could be useful for transplantation of neural progenitor cells at the acute stage of spinal cord injury. Although grafted cells survived only for a short time and did not differentiate into astrocytes or neurons, they were able to reach the parenchyma of the injured spinal cord and improve neurological function in rats. Transplantation efficacy was enhanced at the acute stage in comparison with subacute and chronic stages.

Biliary tract injury caused by different relative warm ischemia time in liver transplantation in rats

BACKGROUND: There is a controversy over the degree of liver and biliary injury caused by the period of secondary warm ischemia. A liver autotransplantation model was adopted because it excludes the effects of infection and immunological rejection on bile duct injury. This study was undertaken to assess biliary tract injury caused by relative warm ischemia (secondary warm ischemia time in the biliary tract) and reperfusion. METHODS: One hundred and two rats were randomly divided into 5 groups: group I (control); groups 11 to V, relative warm ischemia times of 0 minute, 30 minutes, I hour and 2 hours. In addition to the levels of serum alkaline phosphatase, and total bilirubin, pathomorphology assessment and TUNEL assay were performed to evaluate biliary tract damage. RESULTS: Under the conditions that there were no significant differences in warm ischemia time, cold perfusion time and anhepatic phase, group comparisons showed statistically significant differences. The least injury occurred in group H (portal vein and hepatic artery reperfused simultaneously) but the most severe injury occurred in group V (biliary tract relative warm ischemia time 2 hours). CONCLUSIONS: Relative warm ischemia is one of the factors that result in bile duct injury, and the relationship between relative warm ischemia time the bile injury degree is time-dependent. Simultaneous arterial and portal reperfusion is the best choice to avoid the bile duct injury caused by relative warm ischemia. (Hepatobiliary Pancreat Dis Int 2009; 8: 247-254)

Correlation of Surgical Times with Laparoscopic Live Donor Kidney Transplant Outcomes

Most studies revealed that ischemic time has substantial role in occurrence of delayed graft function (DGF) after deceased donor kidney transplantation. However, less is known about the potential impact of surgical times on early outcomes following live donor kidney transplantation. A retrospective cohort of 189 consecutive laparoscopic live donor kidney transplant (LDKT) recipients from January 2006 to August 2012 was analyzed to reveal the impact of pneumoperitoneum time (PT) and anastomosis time (AT) on donor and recipient length of hospital stay and early graft function (EGF). DGF was observed in 13 (6.8%) patients while slow graft function (SGF) was seen in 27 (14%) of the recipients. The median AT was 28 minutes (interquartile range 23, 35 minutes). AT was associated with DGF (Odds Ratio [OR] 1.044, per minute, 95% CI 1.007, 1.082, p = 0.018). Median recipient length of hospital stay was 8 (interquartile range 7, 11) days. Every 13.5 minutes of longer AT was associated with 1 extra day in hospital. The median PT was 180 minutes (interquartile range 144, 234 minutes). PT was associated with both DGF (OR 1.013 per minute, 95% CI 1.005, 1.021, p = 0.001) and SGF (OR 1.009 per minute, 95% CI 1.002, 1.016, p = 0.016). Every extra hour of PT was associated with 0.42 more days in hospital for the donor. Surgical times may be underestimated variables in dictating use of hospital resources. The effect of surgical times on long term hard outcomes entails further study.

茄衣中上部烟叶采收成熟度研究

立足湖北省丹江口市气候特征,以楚雪26号(CX-026)为研究材料,对不同生长时期(移栽后59~101 d)采收的中、上部烟叶的农艺性状、内在生理指标进行分析,探寻烟草成熟的变化规律,从而明晰最佳采收时间。农艺性状结果分析表明,随着采收时间的延后,中部和上部烟叶逐渐变长变宽、身份变厚至恒定水平;叶色由青渐深,后出现黄褐斑纹;茎叶夹角增大、拉力减小,说明烟叶成熟度逐步提高。生理指标分析表明,色素指标(SPAD、叶绿素a等)、光合特性(净光合速率、气孔导度等)随着生长而下降,从而使烟叶叶片色度值(LAB)提高。熵权分析揭示了光合指标和色素指标在烟叶成熟度评估中的重要作用,二者不仅权重高,而且相关性显著,为烟叶成熟度判别提供关键参考。根据光合指标以及色素指标分析结果,中部叶在移栽后66~73 d,上部叶在移栽后87~94 d变化趋于稳定,说明可能在此时间进入成熟期,可以选择作为适宜的采收时间。

心脏移植患者术后尿液管型阳性率及其影响因素

目的分析心脏移植术后患者尿液管型的阳性率及其影响因素。方法收集2023年3月1日—6月31日华中科技大学同济医学院附属协和医院门诊定期复查的143例心脏移植术后患者的尿液样本,行管型镜检,根据镜检结果分为管型阳性组和管型阴性组,比较2个组一般临床资料和相关实验室检测结果差异。采用Logistic回归分析评估心脏移植术后患者尿液管型阳性的影响因素。采用受试者工作特征(ROC)曲线评估相关指标预测心脏移植术后患者尿液管型阳性的效能。结果143例患者尿液管型阳性率为39.2%(56/143)、病理管型阳性率为42.9%(24/56);管型阳性样本中干化学尿蛋白阴性样本占75.0%(42/56)(即易漏检率为75.0%),移植<1年、1~3年、>3年的患者尿液管型阳性率分别为57.5%(23/40)、12.2%(5/41)、45.2%(28/62)。管型阳性组和管型阴性组移植时间、尿素氮、血清肌酐(SCr)、胱抑素C、肾小球滤过率和他克莫司使用情况差异有统计学意义(P<0.05);性别、年龄、糖尿病史、高血压病史、干化学尿蛋白、尿酸、直接胆红素、B型钠尿肽、射血分数、空腹血糖和糖化血红蛋白差异均无统计学意义(P>0.05)。移植时间<1年[比值比(OR)值为5.537,95%可信区间(CI)为1.669~18.372,P=0.001]、移植时间>3年[OR值为7.442,95%CI为2.307~24.009,P=0.005]和他克莫司≥8.3 ng·m L^(–1)[OR值为2.781,95%CI为1.164~6.640,P=0.021]是心脏移植术后患者尿液管型阳性的独立危险因素。多指标联合检测判断心脏移植患者术后尿液管型阳性的曲线下面积为0.810,敏感性为89.3%,特异性为64.4%。结论心脏移植术后患者尿液管型阳性率较高,且易漏检。临床应关注移植时间<1年、移植时间>3年和他克莫司≥8.3 ng·m L^(–1)的心脏移植术后患者尿液管型检出情况。

当归多糖促进骨髓移植重建受体小鼠造血功能

目的探讨当归多糖(ASP)调控骨髓基质细胞(BMSCs)促进供体骨髓移植(BMT)重建受体小鼠造血功能的机制。方法分离纯化8~10周龄雄性C57BL/6J小鼠骨髓单个核细胞(BMMNCs),移植给同龄同种雌性受体小鼠,第9天取受体鼠BMMNCs,再次移植给雌性受体小鼠。移植受体鼠分为对照组:假照射;辐射组:8.0 Gy X射线全身一次性照射;骨髓移植组:照射同辐射组,经尾静脉移植雄性供体BMMNCs(5×10^(6)个细胞);骨髓移植ASP组:同骨髓移植组,从移植的第1天起连续腹腔注射ASP[100 mg/(kg·d)×9]。模型构建期间记录小鼠体重与生存变化,模型构建结束后采集受体小鼠BMMNCs检测Y染色体性别决定区(SRY)基因,测定外周血血常规指标,计算股骨BMMNCs数,观察骨髓组织病理学;分离培养受体鼠BMSCs,观察细胞贴壁生长,5-乙炔基-2’-脱氧尿苷(EdU)法检测BMSCs增殖;分析细胞内活性氧(ROS)水平、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量;检测BMSCs培养上清液中粒细胞-巨噬细胞集落刺激因子(GM-CSF)、干细胞因子(SCF)和胰岛素样生长因子1(IGF-1)水平,检测BMMNCs与各组BMSCs共培养48 h,形成造血祖细胞混合集落(CFU-Mix)数量;Real-time PCR分析受体BMMNCs的Notch信号通路相关基因(Notch1、Jagged1、Hes1)表达。结果单纯辐射组小鼠全部死亡,骨髓移植ASP组受体鼠体重下降不明显;在受体雌鼠BMMNCs中检测到SRY基因;骨髓移植ASP组受体鼠外周血血常规和BMMNCs总数下降幅度不显著,骨髓组织病理学损伤减轻;ASP能促进BMSCs增殖,降低BMSCs中ROS、MDA含量,提高SOD活性;促进BMSCs分泌SCF、GM-CSF及IGF-1,提高BMMNCs与受体BMSCs共培养后的CFU-Mix产率;提高受体小鼠BMMNCs中Notch1、Jagged1、Hes1 mRNA表达。结论ASP促进受体小鼠造血功能重建机制与减轻造血微环境氧化应激损伤,提高BMSCs分泌造血生长因子,调控Notch信号通路有关。

2018—2023年某三甲医院心脏移植术后感染情况及相关危险因素分析

目的 调查2018—2023年某三甲医院心脏移植(HT)术后感染情况,并探讨其危险因素。方法 回顾性分析2018年1月—2023年7月行HT术的314例患者的临床资料,根据术后感染情况将其分为感染组和对照组,记录患者一般信息,对HT术后感染的可能影响因素进行单因素分析,并通过多因素Logistic回归分析筛查HT术后感染的危险因素。结果 91例(28.98%)患者术后发生感染,主要感染部位为呼吸道、血液。Logistic回归分析显示,HT术后感染发生的危险因素主要包括合并慢性肺部疾病、手术时间≥5 h、术后胸腔引流管留置时间长、术后尿管留置时间长、术后机械通气时间长、术前白蛋白(Alb)<35 g/L。结论 合并慢性肺部疾病、手术时间≥5 h、术后胸腔引流管留置时间长、术后尿管留置时间长、术后机械通气时间长、术前Alb<35 g/L与HT术后感染发生有关,故应对患者上述因素进行积极干预,以降低感染风险。

互助县黄芪高产高效栽培技术研究

为了研究适宜互助县推广应用的黄芪高产高效栽培技术,从移栽时间、移栽密度、种植方式、种苗类型4个方面开展了田间试验。结果表明:互助县黄芪最佳的移栽时间为4月中旬,移栽密度为300000株/hm^(2),种植方式为黑地膜全覆膜移栽,种苗类型为直径>0.5 cm、长度>30 cm、根条均匀光滑、无分叉的优质种苗。运用该栽培技术,黄芪的出苗率高、产量高、品质佳、经济效益好。本研究可为互助县大面积推广黄芪种植提供理论依据,促进当地中药材产业的高质量发展。

Real-Time PCR监测巨细胞病毒在肾移植中的应用

目的 :推荐一种快速、敏感、特异的可以早期诊断肾移植受者并发巨细胞病毒 (Cytomegalovirus,CMV)感染的检测技术。方法 :使用实时在线荧光定量PCR(Real-TimePCR)对39名肾移植受者的标本进行CMV— -DNA检测。结果 :39名肾移植受者有17名检出CMV -DNA ,阳性检出率为43 6 % ;116份血浆标本中检出5例阳性 ,中位数 (Md)为5 18×102 拷贝/ml,116份尿液标本中检出13例阳性 ,Md为6 93×102 拷贝/ml,P<0 05 ,未检出同一患者血浆和尿液标本同时阳性的病例。结论 :CMV是肾移植受者中常见的感染 ;尿液与血浆中CMV -DNA检出率的差异具有统计学意义 ;Real-TimePCR是监测肾移植受者CMV感染进程和监测抗病毒药物疗效的有效工具。

超声造影评估裸鼠食管癌移植瘤血管生成情况的实验研究

目的探讨超声造影(CEUS)对裸鼠食管癌移植瘤血管生成情况的评估价值。方法选取18只裸鼠,于其背部皮下注射人食管癌细胞株建立食管癌移植瘤模型,分别于移植后4、6、8周均随机选取6只裸鼠,先应用二维超声观察移植瘤回声、形态和边界,再行CEUS检查并获取时间-强度曲线(TIC)。然后处死所有裸鼠,使用HE染色观察移植瘤细胞结构和组织形态,免疫组化观察移植瘤组织血管内皮生长因子(VEGF)蛋白表达和微血管密度(MVD),比较移植后不同时间移植瘤CEUS表现、TIC定量参数及病理学检查结果的差异。Pearson相关分析法分析TIC定量参数与VEGF蛋白表达、MVD的相关性。结果所有裸鼠食管癌移植瘤模型均成功建立,未出现死亡。移植后4周,移植瘤二维超声表现为类椭圆形低回声肿块,边界清晰,内部回声不均匀,CEUS表现为移植瘤内部呈均匀增强;移植后6周,移植瘤二维超声表现为肿块边界欠清晰,内部回声不均匀,CEUS表现为移植瘤内部呈不均匀高增强;移植后8周,移植瘤二维超声表现为肿块边界不清晰,内部回声不均匀,CEUS表现为移植瘤内部出现灌注缺损。移植后4、6、8周移植瘤峰值强度(PI)、曲线下流入面积(AWI)及曲线下流出面积(AWO)比较,差异均有统计学意义(均P<0.05);与移植后4周比较,移植后6、8周移植瘤PI、AWI和AWO均升高(均P<0.05);与移植后6周比较,移植后8周移植瘤PI和AWO均升高(均P<0.05)。HE染色显示,移植后4周移植瘤见角化珠且细胞间见细胞间桥;移植后6周移植瘤角化珠和细胞间桥均稍减少,毛细血管增多;移植后8周,移植瘤角化珠和细胞间桥均明显减少,毛细血管增多,可见病理性核裂变。免疫组化显示,移植后4、6、8周MVD和VEGF蛋白表达比较,差异均有统计学意义(均P<0.05);与移植后4周比较,移植后6、8周移植瘤MVD均升高,移植后8周移植瘤VEGF蛋白表达升高,差异均有统计学意义(均P<0.05)。相关性分析显示,移植瘤PI、AWI、AWO与MVD、VEGF蛋白表达均呈正相关(均P<0.001)。结论CEUS能够有效观察裸鼠食管癌移植瘤内部血流灌注情况,TIC定量参数PI、AWI、AWO随着移植瘤的生长而逐渐升高,其与移植瘤MVD、VEGF蛋白表达均呈正相关,可以较好地评估移植瘤血管生成情况。

血液病患者异基因造血干细胞移植后Rh血型转变研究

目的检测血液病患者造血干细胞移植(HSCT)前供、受者及移植后患者的Rh血型抗原C、c、E、e,探究受者Rh血型抗原转变为供者Rh血型抗原C、c、E、e转变时间与过程。方法收集HSCT前供、受者以及移植后患者的抗凝全血标本,用微柱凝胶卡检测ABO血型、Rh血型,统计分析并比较ABO、Rh血型抗原转变与时间。结果排除红细胞输注的影响,58例HSCT患者Rh血型抗原C、c、E、e完全转变为供者的Rh血型抗原所需时间为(57.81±8.99)d,患者的年龄和血液病种类影响Rh血型抗原转换时间,性别、移植方式和供受者ABO血型相合性对Rh抗原转变时间无影响。移植后第3周部分患者开始出现少量供者红细胞,第4周开始检测到混和嵌合状态,第7~10周Rh血型抗原完全转变。此外,比较25例供、受者ABO血型和Rh血型均不相同的HSCT患者的Rh血型抗原转变时间和ABO血型转变时间,Rh血型抗原转变时间较ABO更短,差异具有统计学意义。结论定期检测HSCT患者移植后Rh血型抗原可以作为辅助判断移植效果的指标之一,对HSCT患者移植后输注Rh血型相容性的红细胞具有指导意义。

肝移植术后气管导管拔管时间的影响因素

目的分析肝移植术后气管导管延迟拔管的影响因素。方法回顾收集2020年9月1日至2023年3月31日期间在东部战区总医院全身麻醉下行肝移植手术患者的病历资料,根据拔管时间分成为3组,3 h内拔管为早期拔管组、3~24 h之间拔管为延迟拔管组、24 h仍未拔管为拔管困难组。比较3组肝移植受者的临床资料,采用单因素分析和多因素Cox回归分析肝移植术后拔管时间延长的危险因素。结果共纳入237例患者,早期拔管组82例(34.60%)、延迟拔管组119例(50.21%)、拔管困难组36例(15.19%)。单因素分析结果表明3组之间年龄、体重指数(body mass index,BMI)、Child-Pugh评分、MELD评分、无肝期时间、肌松药类型、术中平均体温、再灌注后最低体温具有统计学差异(P<0.05)。多因素Cox回归分析结果显示年龄增加(HR=0.980,95%CI=0.966~0.994,P=0.005)、高MELD评分(HR=0.966,95%CI=0.941~0.991,P=0.009)、术中泵注罗库溴铵是术后拔管时间延长的因素,顺式阿曲库铵与罗库溴铵相比(HR=1.834,95%CI=1.244~2.703,P=0.002)、米库溴铵与罗库溴铵相比(HR=1.660,95%CI=1.073~2.567,P=0.023),平均体温升高(HR=2.447,95%CI=1.449~4.132,P=0.001)和再灌注后最低体温较高(HR=1.288,95%CI=1.023~1.622,P=0.031)为有利于拔管时间缩短。平均体温升高(HR=2.447,95%CI=1.449~4.132,P=0.001)和再灌注后最低体温较高(HR=1.288,95%CI=1.023~1.622,P=0.031)为有利于拔管时间缩短。结论年龄增加、高MELD评分、术中泵注罗库溴铵是术后气管导管拔管时间延长的因素,术中使用顺式阿曲库铵、米库溴铵和积极体温保护有利于术后快速拔管。

宣粳糯7号选育及作麦茬稻种植的高产栽培技术

本文分析了宣粳糯7号的选育过程及其特征特性,并从机插秧和直播两种栽培模式总结其作为麦茬稻的高产栽培技术。选育方面宣粳糯7号是以宣粳糯1号为母本,粳糯86120-5为父本经过系统选育而成的早熟晚粳糯稻品种,具有耐迟播、抗逆性强和适应性广等品种特性,适宜机插秧和直播作麦茬稻种植。作麦茬稻高产栽培技术原则是在有足够的有效穗数基础上,促壮秆、成大穗和增粒重,配合病虫草害防治。具体措施包括适时适量播种、科学肥水管理、综合防治病虫草害和适时收获等,机插秧还要求匀栽密植、插足基本苗。目的在于为该品种的推广种植提供参考。

肺移植受者术后肺动脉高压对体外膜肺氧合时间的影响

目的通过对肺移植术后患者临床资料的回顾性分析,探索不同肺动脉高压患者对肺移植术后患者的机械通气时间和体外膜肺氧合(extracorporeal membrane oxygenation,ECMO)的影响。方法回顾性分析2018年6月2日至2020年5月5日南京医科大学附属无锡人民医院重症医学科纳入101例肺移植患者资料,根据肺移植患者术后不同程度的肺动脉高压,将肺移植术后患者分为62例轻度肺动脉高压组(≤49 mmHg,1 mmHg=0.133 kPa)和39例中重度肺动脉高压组(≥50 mmHg)。运用Cox比例风险模型进行单因素和多因素分析术后不同肺动脉高压组对肺移植术后机械通气时间和ECMO等因素的影响。运用30 d Kaplan-Meiers生存曲线分析不同肺动脉高压组对肺移植术后患者的ECMO时间和机械通气时间的影响。结果轻度、中重度肺动脉高压组的肺移植术后患者在年龄、原发病、肺动脉高压数值、ECMO方式、血红蛋白有显著的统计学差异(P<0.05)。根据术后30 d存活情况比较两组机械通气时间和ECMO时间有显著的统计学差异(P<0.05),单因素Cox回归分析显示,肺移植术后两组的机械通气时间和ECMO时间对生存时间的影响具有统计学意义。多因素Cox回归分析显示肺移植术后患者的机械通气时间对生存时间的影响具有统计学意义。结论肺移植术后中重度肺动脉高压患者需要更长的ECMO时间和机械通气时间。