AIM To establish a liver metastasis model of human colorectal carcinoma in nude mice.METHODS Orthotopic transplantation of histologically intact colorectal tissues from patients into colorectal mucosa of nude mice. Tu...AIM To establish a liver metastasis model of human colorectal carcinoma in nude mice.METHODS Orthotopic transplantation of histologically intact colorectal tissues from patients into colorectal mucosa of nude mice. Tumorgenicity, invasion, metastasis and morphological characteristics of the transplanted tumors were studied by light microscopy, electron microscopy and immunohistochemistry.RESULTS Liver metastasis models of human colon carcinoma (HCA-HMN-1) and human rectal carcinoma (HRA-HMN-2) were established after screening from 34 colorectal carcinomas. They had been passaged in vivo for 18 and 21 generations respectively. There were lymphatic, hemotogenous and implanting metastasesis. CEA secretion was maintained after transplantation. The primary and liver metastatic tumors were similar to the original human carcinoma in histopathological and ultrastructural features, DNA content and chromosomal karyotype.CONCLUSION The liver metastasis models provide useful tools for the study of mechanism of metastasis and its treatment of human colorectal cancer.INTRUDUCTIONSome models of nude mice that fresh human colorectal carcinoma tissue or cells were successfully transplanted subcuteneously have been reported at home and abroad[1,2]. But until now there has been no report on a liver metastasis model of human colorectal carcinoma established by orthotopic transplantation in nude mice in China. Based on our previous models of human liver and pancreas carcinoma by orthotopic transplantation[3,4], we established liver metastasis models of colon and rectum carcinoma with a spontaneous metastasis rate of 100%.展开更多
AIM To elucidate the effect of various solutions for small bowel graft preservation in pigs under hypothermic storage. METHODS The swine segmental small bowel graft was autotransplanted after it was preserved with ...AIM To elucidate the effect of various solutions for small bowel graft preservation in pigs under hypothermic storage. METHODS The swine segmental small bowel graft was autotransplanted after it was preserved with lactated Ringer′s (LR), Euro Collins (EC), hyperosmolarity citrate adenine (HC A) and WMO 1 solutions for 10, 18 and 24 hours, respectively. The recipient survival rate, morphological structure, graft mucosal energy substances and Na + K + ATPase activity were studied, and graft absorption was estimated with D xylose absorption test. RESULTS The morphological study of the grafts preserved with LR or HC A solution for 10 hours or with EC and WMO 1 solution for 18 hours was normal 6 days after operation. Mucosal ATP, total adenine nucleotides (TAN) contents and Na + K + ATPase activity of the graft preserved with EC or WMO solution were higher than that of the graft preserved with LR or HC A solution. Serum level of D xylose was higher in EC and WMO 1 groups than in LR and HC A groups when the graft was preserved for 24 hours. CONCLUSIONS EC and WMO 1 solutions can preserve the swine small bowel up to 18 hours, which are superior to LR and HC A solutions.展开更多
To investigate the effects of metallothionein （MT） on isolated rat heart, 16 Wistar rats were randomly divided into 2 groups. In control group （group C）, distilled water was injected intraperitoneally and 24 h lat...To investigate the effects of metallothionein （MT） on isolated rat heart, 16 Wistar rats were randomly divided into 2 groups. In control group （group C）, distilled water was injected intraperitoneally and 24 h later isolated hearts were perfused with Langendorff and stored at 4℃ for 3 h with histidine-tryptophan-ketoglutarate （HTK） solutions, and then isolated hearts were perfused for 2 h by Langendorff. In experimental group （group E）, 3.6% ZnSO4 was injected intraperitoneally, 24 h later isolated hearts were perfused by Langendorff and stored at 4℃ for 3 h with HTK solutions, and then the isolated hearts were perfused for 2 h with Langendorff. MT content, the recovery of hemodynamics, myocardial water content （MWC）, lactate dehydrogenase （LDH） and creatine kinase （CK） leakage, adenosine triphosphate （ATP） and malondialdehyde （MDA） content, superoxide dismutase （SOD） activity, myocardial cell Ca^2＋ content, Ca^2＋-ATPase activity of mitochondria （[Ca^2＋-ATPase]m） and its Ca^2＋ content （[Ca^2＋]m）, synthesizing ATP activity of mitochondria （[ATP]m）, and the ultrastructure of cells were examined. There were a significant increase in group E in hemodynamic recovery, ATP content, SOD activity, [Ca^2＋-ATPase]m activity, [ATP]m activity, and substantial reduction in MWC, LDH and CK leakage, MDA content, myocardial cell Ca^2＋ content, [Ca^2＋]m content, and the ultrastructural injury were obviously milder than that of group C. This study demonstrated that MT has protective effects on isolated rat heart.展开更多
文摘AIM To establish a liver metastasis model of human colorectal carcinoma in nude mice.METHODS Orthotopic transplantation of histologically intact colorectal tissues from patients into colorectal mucosa of nude mice. Tumorgenicity, invasion, metastasis and morphological characteristics of the transplanted tumors were studied by light microscopy, electron microscopy and immunohistochemistry.RESULTS Liver metastasis models of human colon carcinoma (HCA-HMN-1) and human rectal carcinoma (HRA-HMN-2) were established after screening from 34 colorectal carcinomas. They had been passaged in vivo for 18 and 21 generations respectively. There were lymphatic, hemotogenous and implanting metastasesis. CEA secretion was maintained after transplantation. The primary and liver metastatic tumors were similar to the original human carcinoma in histopathological and ultrastructural features, DNA content and chromosomal karyotype.CONCLUSION The liver metastasis models provide useful tools for the study of mechanism of metastasis and its treatment of human colorectal cancer.INTRUDUCTIONSome models of nude mice that fresh human colorectal carcinoma tissue or cells were successfully transplanted subcuteneously have been reported at home and abroad[1,2]. But until now there has been no report on a liver metastasis model of human colorectal carcinoma established by orthotopic transplantation in nude mice in China. Based on our previous models of human liver and pancreas carcinoma by orthotopic transplantation[3,4], we established liver metastasis models of colon and rectum carcinoma with a spontaneous metastasis rate of 100%.
文摘AIM To elucidate the effect of various solutions for small bowel graft preservation in pigs under hypothermic storage. METHODS The swine segmental small bowel graft was autotransplanted after it was preserved with lactated Ringer′s (LR), Euro Collins (EC), hyperosmolarity citrate adenine (HC A) and WMO 1 solutions for 10, 18 and 24 hours, respectively. The recipient survival rate, morphological structure, graft mucosal energy substances and Na + K + ATPase activity were studied, and graft absorption was estimated with D xylose absorption test. RESULTS The morphological study of the grafts preserved with LR or HC A solution for 10 hours or with EC and WMO 1 solution for 18 hours was normal 6 days after operation. Mucosal ATP, total adenine nucleotides (TAN) contents and Na + K + ATPase activity of the graft preserved with EC or WMO solution were higher than that of the graft preserved with LR or HC A solution. Serum level of D xylose was higher in EC and WMO 1 groups than in LR and HC A groups when the graft was preserved for 24 hours. CONCLUSIONS EC and WMO 1 solutions can preserve the swine small bowel up to 18 hours, which are superior to LR and HC A solutions.
基金This project was supported by a grant from the Qingdao Municipal Sciences and Technology Program (No KZD03-18)
文摘To investigate the effects of metallothionein （MT） on isolated rat heart, 16 Wistar rats were randomly divided into 2 groups. In control group （group C）, distilled water was injected intraperitoneally and 24 h later isolated hearts were perfused with Langendorff and stored at 4℃ for 3 h with histidine-tryptophan-ketoglutarate （HTK） solutions, and then isolated hearts were perfused for 2 h by Langendorff. In experimental group （group E）, 3.6% ZnSO4 was injected intraperitoneally, 24 h later isolated hearts were perfused by Langendorff and stored at 4℃ for 3 h with HTK solutions, and then the isolated hearts were perfused for 2 h with Langendorff. MT content, the recovery of hemodynamics, myocardial water content （MWC）, lactate dehydrogenase （LDH） and creatine kinase （CK） leakage, adenosine triphosphate （ATP） and malondialdehyde （MDA） content, superoxide dismutase （SOD） activity, myocardial cell Ca^2＋ content, Ca^2＋-ATPase activity of mitochondria （[Ca^2＋-ATPase]m） and its Ca^2＋ content （[Ca^2＋]m）, synthesizing ATP activity of mitochondria （[ATP]m）, and the ultrastructure of cells were examined. There were a significant increase in group E in hemodynamic recovery, ATP content, SOD activity, [Ca^2＋-ATPase]m activity, [ATP]m activity, and substantial reduction in MWC, LDH and CK leakage, MDA content, myocardial cell Ca^2＋ content, [Ca^2＋]m content, and the ultrastructural injury were obviously milder than that of group C. This study demonstrated that MT has protective effects on isolated rat heart.
