The purpose of this study is to investigate the function of a novel potassium transporter gene (NrHAK1) isolated from Nicotiana rustica roots using yeast complement and real-time PCR technique. The complementary DNA (...The purpose of this study is to investigate the function of a novel potassium transporter gene (NrHAK1) isolated from Nicotiana rustica roots using yeast complement and real-time PCR technique. The complementary DNA (cDNA) of NrHAK1, 2 488 bp long, contains an open reading frame (ORF) of 2 334 bp encoding a protein of 777 amino acids (87.6 kDa) with 12 predicted transmembrane domains. The NrHAK1 protein shows a high sequence similarity to those of high-affinity potassium transporters in Mesembryanthemum, Phytolacca acinosa, Arabidopsis thaliana, and so on. We found that the NrHAK1 gene could complement the yeast-mutant defect in K+ uptake. Among several tissues surveyed, the expression level of NrHAK1 was most abundant in the root tip and was up-regulated when exposed to potassium starvation. Moreover, the transcript accumulation was significantly reduced by adding 5 mmol/L NH4+ to the solution. These results suggest that NrHAK1 plays an important role in potassium absorption in N. rustica.展开更多
Historical mining activities often lead to continuing wide spread contaminants in both groundwater and surface water in previously operational mine site areas. The contamination may continue for many years after closi...Historical mining activities often lead to continuing wide spread contaminants in both groundwater and surface water in previously operational mine site areas. The contamination may continue for many years after closing down the mining activities. The essential first step for sustainable management of groundwater and development of remediation strategies is the unknown contaminant source characterization. In a mining site, there are multiple species of contaminants involving complex geochemical processes. It is difficult to identify the potential sources and pathways incorporating the chemically reactive multiple species of contaminants making the source characterization process more challenging. To address this issue, a reactive transport simulation model PHT3D is linked to a Simulated Annealing based the optimum decision model. The numerical simulation model PHT3D is utilized for numerically simulating the reactive transport process involving multiple species in the former mine site area. The simulation results from the calibrated PHT3D model are illustrated, with and without incorporating the chemical reactions. These comparisons show the utility of using a reactive, geochemical transport process’ simulation model. Performance evaluation of the linked simulation optimization methodology is evaluated for a contamination scenario in a former mine site in Queensland, Australia. These performance evaluation results illustrate the applicability of linked simulation optimization model to identify the source characteristics while using PHT3D as a numerical reactive chemical species’ transport simulation model for the hydro-geochemically complex aquifer study area.展开更多
One of the most serious and important environmental issues related to the mining sector in Central Queensland is the contamination of abandoned mine sites. Representative of this issue is the abandoned Mount Morgan go...One of the most serious and important environmental issues related to the mining sector in Central Queensland is the contamination of abandoned mine sites. Representative of this issue is the abandoned Mount Morgan gold mine. The potential dispersal of acid mine drainage (AMD), a product of more than 100 million tons of sulphide-rich waste rock, into the surrounding environment, is the most challenging environmental problem currently facing this abandoned mine site. The abandoned Mount Morgan gold mine has multiple pollutant species that involve complex geochemical processes. The present study simulated the flow and transport processes founded on hydrological and geochemical conditions of the real-life field at the mine site. To assess the groundwater contamination risk and detect unknown pollution sources, few chemical species such as Iron and Sulphur were considered as the contaminants. The flow model was simulated using the computer code MODFLOW, and PHT3D was used for the simulation of advection, dispersion and chemical reactions of constituents dissolved in this groundwater system, and to mimic the reactive chemical transport processes in the polluted groundwater. To improve on results from other studies (Datta et al., 2017;Scotney, 2016;Doyle, 2016), a calibrated model was a main focus for this study. Field concentration measurements were matched with the flow simulation outcomes to calibrate the model. The results obtained showed a great potential to model transport of contaminants in the groundwater system using a real-world situation.展开更多
基金(No. 110200101008) supported by the State Tobacco Mo-nopoly Administration, China
文摘The purpose of this study is to investigate the function of a novel potassium transporter gene (NrHAK1) isolated from Nicotiana rustica roots using yeast complement and real-time PCR technique. The complementary DNA (cDNA) of NrHAK1, 2 488 bp long, contains an open reading frame (ORF) of 2 334 bp encoding a protein of 777 amino acids (87.6 kDa) with 12 predicted transmembrane domains. The NrHAK1 protein shows a high sequence similarity to those of high-affinity potassium transporters in Mesembryanthemum, Phytolacca acinosa, Arabidopsis thaliana, and so on. We found that the NrHAK1 gene could complement the yeast-mutant defect in K+ uptake. Among several tissues surveyed, the expression level of NrHAK1 was most abundant in the root tip and was up-regulated when exposed to potassium starvation. Moreover, the transcript accumulation was significantly reduced by adding 5 mmol/L NH4+ to the solution. These results suggest that NrHAK1 plays an important role in potassium absorption in N. rustica.
文摘Historical mining activities often lead to continuing wide spread contaminants in both groundwater and surface water in previously operational mine site areas. The contamination may continue for many years after closing down the mining activities. The essential first step for sustainable management of groundwater and development of remediation strategies is the unknown contaminant source characterization. In a mining site, there are multiple species of contaminants involving complex geochemical processes. It is difficult to identify the potential sources and pathways incorporating the chemically reactive multiple species of contaminants making the source characterization process more challenging. To address this issue, a reactive transport simulation model PHT3D is linked to a Simulated Annealing based the optimum decision model. The numerical simulation model PHT3D is utilized for numerically simulating the reactive transport process involving multiple species in the former mine site area. The simulation results from the calibrated PHT3D model are illustrated, with and without incorporating the chemical reactions. These comparisons show the utility of using a reactive, geochemical transport process’ simulation model. Performance evaluation of the linked simulation optimization methodology is evaluated for a contamination scenario in a former mine site in Queensland, Australia. These performance evaluation results illustrate the applicability of linked simulation optimization model to identify the source characteristics while using PHT3D as a numerical reactive chemical species’ transport simulation model for the hydro-geochemically complex aquifer study area.
文摘One of the most serious and important environmental issues related to the mining sector in Central Queensland is the contamination of abandoned mine sites. Representative of this issue is the abandoned Mount Morgan gold mine. The potential dispersal of acid mine drainage (AMD), a product of more than 100 million tons of sulphide-rich waste rock, into the surrounding environment, is the most challenging environmental problem currently facing this abandoned mine site. The abandoned Mount Morgan gold mine has multiple pollutant species that involve complex geochemical processes. The present study simulated the flow and transport processes founded on hydrological and geochemical conditions of the real-life field at the mine site. To assess the groundwater contamination risk and detect unknown pollution sources, few chemical species such as Iron and Sulphur were considered as the contaminants. The flow model was simulated using the computer code MODFLOW, and PHT3D was used for the simulation of advection, dispersion and chemical reactions of constituents dissolved in this groundwater system, and to mimic the reactive chemical transport processes in the polluted groundwater. To improve on results from other studies (Datta et al., 2017;Scotney, 2016;Doyle, 2016), a calibrated model was a main focus for this study. Field concentration measurements were matched with the flow simulation outcomes to calibrate the model. The results obtained showed a great potential to model transport of contaminants in the groundwater system using a real-world situation.
