Skin photoaging is induced and sustained by UV-induced oxidative damage,and stimulating regeneration of the UV-induced aging has remained a great challenge due to high-level oxidative stress factor(ROS)-induced chroni...Skin photoaging is induced and sustained by UV-induced oxidative damage,and stimulating regeneration of the UV-induced aging has remained a great challenge due to high-level oxidative stress factor(ROS)-induced chronic oxidative damage and inactivation of bio-macromolecule-based regeneration in oxidative photoaging micro-environment.In this study,we designed a“seed and soil”strategy to pursue a safer and more efficient way to prevent and treat photoaging by simultaneously changing UV-induced ROS-rich micro-environment into a proregenerative one(the“soil”)and providing growth factor-rich platelet lysates(PL,the“seed”)using PL-impregnated,collagen-reinforce hydrogel(PL/Col).SD rats were used to establish photoaging model by 8 weeks of UV irradiation.The effectiveness of different treatments was evaluated by making pathological sections and detecting photoaging-related indicators.Rats treated with PL/Col demonstrated a significant acceleration in skin healing and enhancement in the quality of trauma repairing.After treated with PL/Col,the rats showed smooth yellowish appearance,integral structure of skin collagen fiber and epidermis,a decrease in inflammation and a reshaped active micro-environment with reduced levels of SOD enzyme activity,GSH enzyme activity and MDA toxic products.Treatment of PL/Col in skin photoaging has shown potential anti-oxidation and anti-aging effects and is worthy of further study in related field.展开更多
Traumatic spinal cord injury（SCI）,defined as physical trauma to the spinal column yielding altered motor,sensory,or autonomic function,is a devastating neurological disease causing major impact at both personal and ...Traumatic spinal cord injury（SCI）,defined as physical trauma to the spinal column yielding altered motor,sensory,or autonomic function,is a devastating neurological disease causing major impact at both personal and societal level.SCI is characterized by a primary insult（compression,contusion or laceration）followed by a secondary pathologicalcascade that propagates further injury disrupting motor,展开更多
Bone marrow, the vital organ which maintains lifelong hemopoiesis, currently receives considerable attention, as a source of multiple cell types which may play important roles in repair at distant sites. This emerging...Bone marrow, the vital organ which maintains lifelong hemopoiesis, currently receives considerable attention, as a source of multiple cell types which may play important roles in repair at distant sites. This emerging function, distinct from, but closely related to, bone marrow roles in innate immunity and inflammation, has been characterized through a number of strategies. However, the use of surgical models in this endeavour has hitherto been limited. Surgical strategies allow the experimenter to predetermine the site, timing, severity and invasiveness of injury; to add or remove aggravating factors(such as infection and defects in immunity) in controlled ways; and to manipulate the context of repair, including reconstitution with selected immune cell subpopulations. This endows surgical models overall with great potential for exploring bone marrow responses to injury, inflammation and infection, and its roles in repair and regeneration. We review three different murine surgical models, which variously combine trauma with infection, antigenic stimulation, or immune reconstitution, thereby illuminating different aspects of the bone marrow response to systemic injury in sepsis, trauma and allergy. They are:(1) cecal ligation and puncture, a versatile model of polymicrobial sepsis;(2) egg white implant, an intriguing model of eosinophilia induced by a combination of trauma and sensitization to insoluble allergen; and(3) ectopic lung tissue transplantation, which allows us to dissect afferent and efferent mechanisms leading to accumulation of hemopoietic cells in the lungs. These models highlight the gain in analytical power provided by the association of surgical and immunological strategies.展开更多
A large number of loose piles formed by mountain hazards are highly susceptible to hydraulic erosion under rainfall conditions.The use of ecological substrate materials for erosion control and ecological restoration o...A large number of loose piles formed by mountain hazards are highly susceptible to hydraulic erosion under rainfall conditions.The use of ecological substrate materials for erosion control and ecological restoration of gravel soil slopes has become a current research hotspot and the study difficulty.The post-earthquake slump accumulation gravel soil in Jiuzhaigou was selected as the research object,and the self-developed modified glutinous rice-based material was used to reinforce the gravel soil.The variable slope flume erosion test and rainfall simulation test were carried out to study the water erosion resistance of the material reconstructed soil under the influence of runoff erosion and raindrop splash erosion.The results show that:As the material content reached 12.5%,the reconstructed soil did not disintegrate after 24 hours of immersion,the internal friction angle was increased by 42.26%,and the cohesion was increased by 235.5%,which played a significant reinforcement effect.In the process of slope erosion,the soil rill erodibility parameter Kr was only 3‰ of the gravel soil control group,the critical shear force τ increased by 272%,and the soil erosion resistance was significantly improved.In the process of rainfall and rainfall on the slope,the runoff intensity of the reconstructed soil was stable,and the ability to resist runoff erosion and raindrop splash erosion was enhanced.The maximum value of soil loss rate on different slope slopes is 0.02-0.10 g·m^(-2)s^(-1),which is significantly lower than that of the control group and has better erosion reduction effect.展开更多
基金funding from the Discipline construction project of Guangdong Medical University(4SG21277P,2021ZDZX2039,2DK21003,4SG23060G)Our work described in the present manuscript was also supported by research grants from Guangdong Provincial Natural Science Foundation(2021A1515012437,2021KTSCX040),Guangdong,China。
文摘Skin photoaging is induced and sustained by UV-induced oxidative damage,and stimulating regeneration of the UV-induced aging has remained a great challenge due to high-level oxidative stress factor(ROS)-induced chronic oxidative damage and inactivation of bio-macromolecule-based regeneration in oxidative photoaging micro-environment.In this study,we designed a“seed and soil”strategy to pursue a safer and more efficient way to prevent and treat photoaging by simultaneously changing UV-induced ROS-rich micro-environment into a proregenerative one(the“soil”)and providing growth factor-rich platelet lysates(PL,the“seed”)using PL-impregnated,collagen-reinforce hydrogel(PL/Col).SD rats were used to establish photoaging model by 8 weeks of UV irradiation.The effectiveness of different treatments was evaluated by making pathological sections and detecting photoaging-related indicators.Rats treated with PL/Col demonstrated a significant acceleration in skin healing and enhancement in the quality of trauma repairing.After treated with PL/Col,the rats showed smooth yellowish appearance,integral structure of skin collagen fiber and epidermis,a decrease in inflammation and a reshaped active micro-environment with reduced levels of SOD enzyme activity,GSH enzyme activity and MDA toxic products.Treatment of PL/Col in skin photoaging has shown potential anti-oxidation and anti-aging effects and is worthy of further study in related field.
文摘Traumatic spinal cord injury（SCI）,defined as physical trauma to the spinal column yielding altered motor,sensory,or autonomic function,is a devastating neurological disease causing major impact at both personal and societal level.SCI is characterized by a primary insult（compression,contusion or laceration）followed by a secondary pathologicalcascade that propagates further injury disrupting motor,
文摘Bone marrow, the vital organ which maintains lifelong hemopoiesis, currently receives considerable attention, as a source of multiple cell types which may play important roles in repair at distant sites. This emerging function, distinct from, but closely related to, bone marrow roles in innate immunity and inflammation, has been characterized through a number of strategies. However, the use of surgical models in this endeavour has hitherto been limited. Surgical strategies allow the experimenter to predetermine the site, timing, severity and invasiveness of injury; to add or remove aggravating factors(such as infection and defects in immunity) in controlled ways; and to manipulate the context of repair, including reconstitution with selected immune cell subpopulations. This endows surgical models overall with great potential for exploring bone marrow responses to injury, inflammation and infection, and its roles in repair and regeneration. We review three different murine surgical models, which variously combine trauma with infection, antigenic stimulation, or immune reconstitution, thereby illuminating different aspects of the bone marrow response to systemic injury in sepsis, trauma and allergy. They are:(1) cecal ligation and puncture, a versatile model of polymicrobial sepsis;(2) egg white implant, an intriguing model of eosinophilia induced by a combination of trauma and sensitization to insoluble allergen; and(3) ectopic lung tissue transplantation, which allows us to dissect afferent and efferent mechanisms leading to accumulation of hemopoietic cells in the lungs. These models highlight the gain in analytical power provided by the association of surgical and immunological strategies.
基金jointly funded by the Sichuan Provincial Natural Science Foundation of China (Grant No.2023NSFSC0378)the Jiuzhaigou Lake Swamp and River Ecological Restoration Research Project (N5132112022000246)。
文摘A large number of loose piles formed by mountain hazards are highly susceptible to hydraulic erosion under rainfall conditions.The use of ecological substrate materials for erosion control and ecological restoration of gravel soil slopes has become a current research hotspot and the study difficulty.The post-earthquake slump accumulation gravel soil in Jiuzhaigou was selected as the research object,and the self-developed modified glutinous rice-based material was used to reinforce the gravel soil.The variable slope flume erosion test and rainfall simulation test were carried out to study the water erosion resistance of the material reconstructed soil under the influence of runoff erosion and raindrop splash erosion.The results show that:As the material content reached 12.5%,the reconstructed soil did not disintegrate after 24 hours of immersion,the internal friction angle was increased by 42.26%,and the cohesion was increased by 235.5%,which played a significant reinforcement effect.In the process of slope erosion,the soil rill erodibility parameter Kr was only 3‰ of the gravel soil control group,the critical shear force τ increased by 272%,and the soil erosion resistance was significantly improved.In the process of rainfall and rainfall on the slope,the runoff intensity of the reconstructed soil was stable,and the ability to resist runoff erosion and raindrop splash erosion was enhanced.The maximum value of soil loss rate on different slope slopes is 0.02-0.10 g·m^(-2)s^(-1),which is significantly lower than that of the control group and has better erosion reduction effect.
