We investigated the effects of graphene on the model herb Artemisia annua,which is renowned for produc-ing artemisinin,a widely used pharmacological compound.Seedling growth and biomass were promoted when A.annua was ...We investigated the effects of graphene on the model herb Artemisia annua,which is renowned for produc-ing artemisinin,a widely used pharmacological compound.Seedling growth and biomass were promoted when A.annua was cultivated with low concentrations of graphene,an effect which was attributed to a 1.4-fold increase in nitrogen uptake,a 15%–22%increase in chlorophyllfluorescence,and greater abun-dance of carbon cycling–related bacteria.Exposure to 10 or 20 mg/L graphene resulted in a�60%increase in H2O2,and graphene could act as a catalyst accelerator,leading to a 9-fold increase in catalase(CAT)ac-tivity in vitro and thereby maintaining reactive oxygen species(ROS)homeostasis.Importantly,graphene exposure led to an 80%increase in the density of glandular secreting trichomes(GSTs),in which artemisinin is biosynthesized and stored.This contributed to a 5%increase in artemisinin content inmature leaves.Inter-estingly,expression of miR828 was reduced by both graphene and H2O2 treatments,resulting in induction of its target gene AaMYB17,a positive regulator of GST initiation.Subsequent molecular and genetic assays showed that graphene-induced H2O2 inhibits micro-RNA(miRNA)biogenesis through Dicers and regulates the miR828–AaMYB17 module,thus affecting GST density.Our results suggest that graphene may contribute to yield improvement in A.annua via dynamic physiological processes together with miRNA regulation,and it may thus represent a new cultivation strategy for increasing yield capacity through nanobiotechnology.展开更多
基金supported by the National Natural Science Foundation of China (82274047 and 52071192)the National Key R&D Program of China (2018YFA0900600)+3 种基金the Engineering Research Center of Coal-Based Ecological Carbon Sequestration Technology of the Ministry of Education (MJST2023-3)the China Postdoctoral Science Foundation (2023M732232)SJTU Trans-med Awards Research (20190104)supported in part by the Bill&Melinda Gates Foundation (OPP1199872 and INV-027291).
文摘We investigated the effects of graphene on the model herb Artemisia annua,which is renowned for produc-ing artemisinin,a widely used pharmacological compound.Seedling growth and biomass were promoted when A.annua was cultivated with low concentrations of graphene,an effect which was attributed to a 1.4-fold increase in nitrogen uptake,a 15%–22%increase in chlorophyllfluorescence,and greater abun-dance of carbon cycling–related bacteria.Exposure to 10 or 20 mg/L graphene resulted in a�60%increase in H2O2,and graphene could act as a catalyst accelerator,leading to a 9-fold increase in catalase(CAT)ac-tivity in vitro and thereby maintaining reactive oxygen species(ROS)homeostasis.Importantly,graphene exposure led to an 80%increase in the density of glandular secreting trichomes(GSTs),in which artemisinin is biosynthesized and stored.This contributed to a 5%increase in artemisinin content inmature leaves.Inter-estingly,expression of miR828 was reduced by both graphene and H2O2 treatments,resulting in induction of its target gene AaMYB17,a positive regulator of GST initiation.Subsequent molecular and genetic assays showed that graphene-induced H2O2 inhibits micro-RNA(miRNA)biogenesis through Dicers and regulates the miR828–AaMYB17 module,thus affecting GST density.Our results suggest that graphene may contribute to yield improvement in A.annua via dynamic physiological processes together with miRNA regulation,and it may thus represent a new cultivation strategy for increasing yield capacity through nanobiotechnology.
