Triple-helix knowledge-based Sino-Nordic Arctic relationships for trust and sustainable development

This paper goes beyond Sino-Nordic Arctic science diplomacy and looks at Sino-Nordic Arctic triple-helix knowledge- based collaborations among academia, business, civil society （the inclusion of which moves beyond the original triple-helix concept）, and government. In light of the potential of science diplomacy for building Sino-Arctic trust under systemic international conditions of power transition and globalization, this is the natural next step toward exploring triple-helix collaborations. Knowledge-based collaborations between academia, business, civil society, and government also open up avenues for innovation and entrepreneurship by both Arctic societies and Chinese society in attempts to address major challenges to sustainable development in these societies. This paper discusses possible triple-helix knowledge-based collaborations with China by each of the five Nordic countries, and highlights the innovation and entrepreneurial talents of summer school students at the University of International Relations in Beijing in developing projects as part of a course entitled ＂The Global Arctic＂.

Label-free fluorescent strategy for sensitive detection of tetracycline based on triple-helix molecular switch and G-quadruplex

In this assay, a label-free fluorescent sensing platform based on triple-helix molecular switch（THMS） and G-quadruplex was developed for the detection of tetracycline. We demonstrated this approach by using THMS, which consists of a central section with a shortened 8-mer aptamer sequence with high affinity to tetracycline and flanked by two arm segments. G-rich oligonucleotide can specifically bind to thioflavin T（Th T） as a signal transduction probe（STP）. In the absence of tetracycline, THMS remains stable, the fluorescence of background is low. By the addition of target tetracycline, the aptamer-target binding results in the formation of a structured aptamer-target complex, which disassembles the THMS and releases the STP. The free STP self-assembles into G-quadruplex and specifically binds to Th T which generates a obvious fluorescence enhancement. Using the triple-helix molecular switch, the developed aptamer-based fluorescent sensing platform showed a linear relationship with the concentration of tetracycline ranging from 0.2 to 20.0 nmol/L. The detection limit of tetracycline was determined to be970.0 pmol/L. The assay avoids complicated modifications or chemical labeling, making it simple and cost-effective. So, it is expected that this aptamer-based fluorescent assay could be extensively applied in the field of food safety inspection.